Re: [DIYbio] Promoter Testing

Can you post the data? How does the data look when you sort by
emission counts then plot it?

On Thu, May 14, 2015 at 4:02 PM, Linden <ff.lindenberg@gmail.com> wrote:
> Hi guys!
>
> I would like to rank in strength a few promoters I have. The promoters are
> cloned upstream GFP, so I used a spectrophotometer (485 nm excitation 525 nm
> emission) to measure the GFP in E. coli, just like the paper "Measuring the
> activity of BioBrick promoters using an in vivo reference standard". I did
> everything, and now I have a some numbers, but I don't know how to turn it
> in something useful. Anyone know which formula should I use to get a
> estimate of the GFP or mRNA concentration inside the cell?
>
> Thanks a lot!
>
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--
-Nathan

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