Re: [DIYbio] Obtaining J. lividum or another non-pathogenic violacein producer

I want to build a bioreactor for producing Violacein,

Alan

On May 8, 2015, at 5:48 AM, Claudio Tiecher <ctie...@gmail.com> wrote:

Hi Alan,

Officially to get the biobrick you have to be registered as a iGEM lab. To register the iGEM lab you need to affiliate to an institution/community/company.

Usually iGEM lab are really helpful. Find the closest iGEM lab and ask them to get the biobricks for you.

May I ask what is it your purpose?

Cheers,
Claudio

On Thursday, May 7, 2015 at 8:44:50 PM UTC+2, Alan WiIliams wrote:

Hi,

So I might play with the pathway later, but for right now I would like a working plasmid.  The biobrick looks fine for my purposes, does anyone know how to get those as an individual?

Thanks,

Alan

On May 7, 2015, at 1:53 PM, Brian Degger <brian....@gmail.com> wrote:

Nathan, 

Yes, you are right. It was bits for a pathway, not a plasmid as such.

However, I wasn't sure if Alan wanted to play with the pathway. I have also have also seen that http://parts.igem.org/Part:BBa_K274002?title=Part:BBa_K274002 produces violacein from l-tryptophane in E.coli  

On Thu, May 7, 2015 at 6:17 PM, Nathan McCorkle <nmz...@gmail.com> wrote:

On Thu, May 7, 2015 at 6:04 AM, Brian Degger <brian....@gmail.com> wrote:
> Last year, synbiota/genomicon was shipping the violacein pathway
> (https://synbiota.com/dna-market/8) for transforming into e. coli k12

Wasn't that more of a kit to try hacking the pathway into working,
rather than a plasmid with a working pathway?

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diy...@googlegroups.com. To unsubscribe from this group, send email to diybio+un...@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+un...@googlegroups.com.
To post to this group, send email to diy...@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CA%2B82U9KLBo7Bfi%3DYad2HnjhsaCWrVz-QruMjsrpDptBWHiAJ2w%40mail.gmail.com.

For more options, visit https://groups.google.com/d/optout.

--

----------------------------------------
Brian Degger

twitter: @drbrian

http://makerspace.org.uk

http://transitlab.org

----------------------------------------

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diy...@googlegroups.com. To unsubscribe from this group, send email to diybio+un...@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+un...@googlegroups.com.
To post to this group, send email to diy...@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CACc%3DpRLvhG2w5puYnXsaKBFLwMKk7Hvpg%2BqbEZ%3DFENK-yn_KxA%40mail.gmail.com.
For more options, visit https://groups.google.com/d/optout.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diy...@googlegroups.com. To unsubscribe from this group, send email to diybio+un...@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+un...@googlegroups.com.
To post to this group, send email to diy...@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/84bf8896-6b5c-48ca-921b-784b6126db4f%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.