Re: [DIYbio] Obtaining J. lividum or another non-pathogenic violacein producer

Hi Alan,

that is an awesome project. I have thought about producin violacein myself. I have done quite some research about it and have quite some literature. I can share more information here or privately... I also tried to get the strain from an Indonesian university, but it did not work.

What is your experience with biology? As far as I remember there are many different non-pathogenic organisms you can choose.

Cheers,
Claudio

On Friday, May 8, 2015 at 3:24:09 PM UTC+2, Alan WiIliams wrote:

I want to build a bioreactor for producing Violacein,

Alan

On May 8, 2015, at 5:48 AM, Claudio Tiecher <ctie...@gmail.com> wrote:

Hi Alan,

Officially to get the biobrick you have to be registered as a iGEM lab. To register the iGEM lab you need to affiliate to an institution/community/company.

Usually iGEM lab are really helpful. Find the closest iGEM lab and ask them to get the biobricks for you.

May I ask what is it your purpose?

Cheers,
Claudio

On Thursday, May 7, 2015 at 8:44:50 PM UTC+2, Alan WiIliams wrote:

Hi,

So I might play with the pathway later, but for right now I would like a working plasmid.  The biobrick looks fine for my purposes, does anyone know how to get those as an individual?

Thanks,

Alan

On May 7, 2015, at 1:53 PM, Brian Degger <brian....@gmail.com> wrote:

Nathan, 

Yes, you are right. It was bits for a pathway, not a plasmid as such.

However, I wasn't sure if Alan wanted to play with the pathway. I have also have also seen that http://parts.igem.org/Part:BBa_K274002?title=Part:BBa_K274002 produces violacein from l-tryptophane in E.coli  

On Thu, May 7, 2015 at 6:17 PM, Nathan McCorkle <nmz...@gmail.com> wrote:

On Thu, May 7, 2015 at 6:04 AM, Brian Degger <brian....@gmail.com> wrote:
> Last year, synbiota/genomicon was shipping the violacein pathway
> (https://synbiota.com/dna-market/8) for transforming into e. coli k12

Wasn't that more of a kit to try hacking the pathway into working,
rather than a plasmid with a working pathway?

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