Re: [DIYbio] Does anyone know how to inexpensively obtain gluconacetobacter xylinus?

IIRC (it was a while ago) G.xylinus is a dark brown and has little
distinctive shape; flat and brownish sounds about right. The
glossy/clear partner sounds like it might be a yeast.

Take pickings from distinct, separate examples of both colonies and
streak them out again on separate agar, you'd expect the brown guys to
*prodigiously* clear the CaCO3 in the agar and make it
clear/see-through, and in late-stage culture (+7 days or even later) to
see chunks of cellulose forming near, though not necessarily *on*,
colonies. That was my experience, at least.

As for the white guys, check 'em under a microscope; if they're yeast,
they're probably much larger than bacteria and easy to observe, for
starters. You might even see nuclei without a stain, though try
methylene blue for a cheap, cheerful DNA stain.

On 01/07/15 20:47, Philipp Kollenz wrote:
> I have two different types of colonies:
> - Flat and brownish
> - "Bumpy", glossy, and almost clear
>
> They both aren't yeasts (as far as I could tell with my microscope) and
> grew after 5-6 days at 26°C
> Which of them is the one I'm looking for?
>
> Am Sonntag, 7. Juni 2015 19:33:09 UTC+2 schrieb Cathal (Phone):
>
> It's easy to isolate from Kombucha, and some literature suggests
> this strain gives the best cellulose, too. :)
>
> Just make up "Gluconobacter agar" (look it up on thelabrat.com
> <http://thelabrat.com>) and streak your kombucha culture over it
> thinly. When I isolated it in this way, my colonies were brown,
> cleared the CaCO3 quickly, and in *late* culture, white crystals (of
> cellulose?) appeared around, but rarely atop, colonies.
>
> On 7 June 2015 12:37:54 GMT+01:00, Philipp Kollenz
> <philipp...@googlemail.com <javascript:>> wrote:
>
> Hi there
>
> I am trying to produce nanocellulose and the cultures you can
> buy on websites like this one
> (https://www.dsmz.de/catalogues/details/culture/DSM-2325.html
> <https://www.dsmz.de/catalogues/details/culture/DSM-2325.html>)
> are too expensive. I heard it is part of a microbe mix that is
> used to make some kind of asiatic tea beverage called Kombucha.
> Is it possible to isolate it from there?
>
> best regards,
> Phil
>
>
> --
> Sent from my Android device with K-9 Mail. Please excuse my brevity.
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--
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