This is pretty cool. If people try and repeat these experiments can they post detailed details(heh) of what they did so others can follow suite.
Also, it doesn't seem anyone is running the control of everything but the SLiCE mixture. If you are running an experiment can you do that and let us know how that goes?On Thu, Oct 29, 2015 at 2:44 PM, Koeng <koeng101@gmail.com> wrote:
I tried out my homemade SLiCE mix. I put 100ng of both insert and vector in for a 2 piece assembly, for 30 minutes. I compared it to some homemade Gibson mix.
On my plates, I got 13 colonies on the SLiCE plate and 42 colonies on the Gibson. I am extremely impressed with 1/3 considering just the simple strain and simple lysis procedure.
-Koeng
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