I tried out my homemade SLiCE mix. I put 100ng of both insert and vector in for a 2 piece assembly, for 30 minutes. I compared it to some homemade Gibson mix.
On my plates, I got 13 colonies on the SLiCE plate and 42 colonies on the Gibson. I am extremely impressed with 1/3 considering just the simple strain and simple lysis procedure.
-Koeng
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Re: [DIYbio] Re: The ODIN Needs Your Help - T4 DNA Ligase Testing - Free Ligase
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