Re: [DIYbio] Re: Electroporation vs Sonification vs CaCl Chlorella Vulgrais

On 12/17/2015 12:00 PM, Alex D wrote:
> i have seen papers where people have used 150 200 volts but i think for the longer time or smaller cuvette? I proposed to give it
> a shot for a minute or two in inside the gell in the electrophoretic unit and then check the results but

Longer as in minutes is probably just going to cook/kill most anything.
2kV is the high end of the range for non-salty suspensions, so safety is the hurdle.

I'm working on ways to get low volumes of plastic parts "molded by John" and that will lead
to some lab gear with safety interlocks in a year maybe.

You could try using a barbeque grill piezo spark lighter and various R and C to get different volt levels
to your cuvette, but you sound impatient and "try anything", so I have to say "Please don't -- so you don't
forget and grab the thing being electrocuted with one hand and the spark hot wire with the other and
give yourself an arm to arm shot that puts your heart in fibrillation leading to death."

Instead of getting in big trouble, buy the Biorad Gene pulser here: $175 http://www.ebay.com/itm/like/221303599697

$39 (needs work) http://www.ebay.com/itm/Bio-Rad-Gene-Pulser-Laboratory-High-Voltage-Electrophoresis-Unit-1652076-/361451197470

$85 untested http://www.ebay.com/itm/BIO-RAD-GENE-PULSER-/262178386007?hash=item3d0b0cb057

$139 extra huge cap bank: http://www.ebay.com/itm/Bio-Rad-Capacitance-Extender-1652087-Gene-Pulser-Electrophoresis/201094768798

Be sure to read the destructions even with the safety engineered Biorad machines. Those cables between can be deadly.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/5673FA08.7020305%40industromatic.com.
For more options, visit https://groups.google.com/d/optout.