Re: [DIYbio] Re: Is there interest in lower cost restriction enzyme varieties?

I can has NcoI? o.O

These prices are awesome! I'm a huge fan of traditional cloning and wish more people would cut the whole expensive gibson, splice, goldengate bs and just learn using reliable dual cutter cloning. Its like saying "I want to learn to drive a car but first I must purchase a Bentley". Surprised you don't carry EcoRI and the other biobrick standards as they are also super handy for diagnostic cutting post mini-prep and should be cheap as all hell. I use EcoRI daily for routine diagnostic cuts of my supposed clones and NEB has me by the...belt...with costs. I would be happy to review the parts for you for some kind of discount if you would like. I literally only do restriction cloning in my work. It may be slow but its reliable as hell and that's very hard to come by nowadays...

On Thu, Dec 17, 2015 at 2:35 PM, lareine <OAde...@gmail.com> wrote:

We sell high quality restriction enzymes at the fraction of the NEB price: http://www.aasinc.co/store-and-promotions.html#!/c/0/offset=0&sort=normal

On Thursday, November 13, 2014 at 7:06:58 PM UTC-5, Justin wrote:

Hi everyone, I'm just trying to get a feel for who out there in DIYBio land might be interested in something like this. As it is right now the cost of restriction enzymes is anywhere between $55 - $200 per 10,000 units. I was thinking if your an independent scientist or working in a community lab 10,000 units might be a bit of overkill and would last an extremely long time. So I'm trying to see if there is interest out there for an option to purchase 500 - 1000 units of restriction enzymes for about $10? My thinking is that while it would be higher cost/unit the cost of having a variety of restriction enzymes would be immensely reduced.

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