I'm trying to culture persister borrelia on peripheral nerve tissue so that I can submit the bacteria cells for proteomics analysis.
On Monday, January 25, 2016 at 8:48:22 AM UTC-5, Reshma Bhatnagar wrote:
-- On Monday, January 25, 2016 at 8:48:22 AM UTC-5, Reshma Bhatnagar wrote:
"I'm trying to adapt an existing procedure for culturing bacteria to instead culture the bacteria on nerve tissue"
Why are you trying to grow bacteria on nerve tissue? Seems wasteful and expensive. If culturing bacteria is your concern, there are several media that can be used and are widely available. At the end of the day, the nutritional requirements of the bacteria need to be met in order to culture them, and I don't see any special reason to use nerve tissue to culture any bacteria.
What is the goal for culturing your bacteria?
If you are attempting to do so to explore treatments of bacterial infections that affect the brain by culturing bacteria on nerve tissue and using drugs to kill the bacteria, the attempt would be meaningless because:
1. Both need to pass the blood brain barrier in living organisms
2. Antibiotics have been well studied anyway
3. Organisms may be resistant to the antibiotics you test
If you want to go ahead anyway, I suggest buying offal/animal brains from a butcher. I will however, reiterate that it seems pointless. Perhaps, if you could outline what you propose to do, I can come up with a more helpful reply.
On Monday, January 25, 2016 at 6:11:57 AM UTC+5:30, coolcash2004 wrote:Anyone have experience working with nerve tissue? Where can you buy it? Any major challenges I should be aware of? I'm trying to adapt an existing procedure for culturing bacteria to instead culture the bacteria on nerve tissue. Thanks again,
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