Re: [DIYbio] Re: Where to buy nerve tissue?

I'd look into co-culturing with something like one of these
devices(SIMpore cytoVu, produced by ibidi):
http://www.sigmaaldrich.com/catalog/product/sigma/smp0005?lang=en&region=US

On Mon, Jan 25, 2016 at 4:28 PM, coolcash2004 <davidconwayj@gmail.com> wrote:
> I'm trying to culture persister borrelia on peripheral nerve tissue so that
> I can submit the bacteria cells for proteomics analysis.
>
> On Monday, January 25, 2016 at 8:48:22 AM UTC-5, Reshma Bhatnagar wrote:
>>
>> "I'm trying to adapt an existing procedure for culturing bacteria to
>> instead culture the bacteria on nerve tissue"
>>
>> Why are you trying to grow bacteria on nerve tissue? Seems wasteful and
>> expensive. If culturing bacteria is your concern, there are several media
>> that can be used and are widely available. At the end of the day, the
>> nutritional requirements of the bacteria need to be met in order to culture
>> them, and I don't see any special reason to use nerve tissue to culture any
>> bacteria.
>>
>> What is the goal for culturing your bacteria?
>>
>> If you are attempting to do so to explore treatments of bacterial
>> infections that affect the brain by culturing bacteria on nerve tissue and
>> using drugs to kill the bacteria, the attempt would be meaningless because:
>>
>> 1. Both need to pass the blood brain barrier in living organisms
>> 2. Antibiotics have been well studied anyway
>> 3. Organisms may be resistant to the antibiotics you test
>>
>> If you want to go ahead anyway, I suggest buying offal/animal brains from
>> a butcher. I will however, reiterate that it seems pointless. Perhaps, if
>> you could outline what you propose to do, I can come up with a more helpful
>> reply.
>>
>> On Monday, January 25, 2016 at 6:11:57 AM UTC+5:30, coolcash2004 wrote:
>>>
>>> Anyone have experience working with nerve tissue? Where can you buy it?
>>> Any major challenges I should be aware of? I'm trying to adapt an existing
>>> procedure for culturing bacteria to instead culture the bacteria on nerve
>>> tissue. Thanks again,
>
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-Nathan

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