I think David's suggestion is good. Radiation resistance would probably translate better between mammal species than from bacteria to mammals. You might want to look at tardigrades as a source. While not mammals, they are at least animals, so the mechanisms of radiation resistance would be much more likely to work in a mammal context than a bacterial mechanism. I've attached a paper about tardigrade radiation tolerance.
-- On Thu, Feb 4, 2016 at 3:12 AM David Weichselbaum <david.t.weichselbaum@gmail.com> wrote:
First you should know what you want to do in detail (on the level of base pairs) and then you can think about transforming mammals.If you know what you're doing however, you could use a pEPito mammalian vector, which is basically a small artificial chromosome that piggybacks on native chromosomes to be mitotically stable.Then you can gather rat sperm (maybe using a syringe and anesthetics is less stressful for both you and the rat (-: ) and use the sperm mediated gene transfer protocol. This is basically centrifuging the sperm, mixing it with the vector in the right buffer and injecting it into your rat mama (you really should read up on lab animal ethics and consult specialists to help you). The efficiency of getting transgenic offspring is 80% in pigs.Have you considered researching radiation resistance in mammals, not in bacteria? As everyone keeps saying, it's just the level of DNA repair you're after.Get a permit for the exclusion zone of Chernobyl, Ukraine und take some live traps with you. The red forest is one of the most radioactive places on earth and while you cannot enter it, you should be able to get some really radioactive rats in its vicinity.Take some blood and tissue samples and do an expression analysis or amplify and sequence known DNA-repair mechanisms. I bet after 30 years and hundreds of generations they are nicely adapted to their new home. After that you should be able to reverse-engineer their resistance or even amplify it.--
On Thursday, January 28, 2016 at 9:11:22 PM UTC+1, Finn Daffron wrote:I am trying to create a batch of thermococcus gammatolerans. This is because I want to use CRISPR to inject thermococcus gammatolerans into rats in order to create radiation resistance with possible implications being people working in nuclear power plants, astronauts, etc. Does anyone have any ideas on how it would be synthesized? Please get back to me.
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