[DIYbio] Re: PCR trouble

No I'm using Plasmid from a miniprep as my template.

I'm also using a premade Taq Master mix and 1pmol per uL of each primer in the final reaction. My reactions are 25uL and I'm doing them on a plate.

Also Tom, I did see those other sites in the section I want, I have a long convoluted plan to put everything back together, hopefully that works better than my PCR so far.

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