Are you linearizing the plasmid with an enzyme that cut outside of your target before you amplify? The backbone of pVIB is pBR322. You can get more details on how it is put together here.
Cheers,
Scott
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/8f4b432e-424a-4e4b-9d25-846b7d796160%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.
0 comments:
Post a Comment