My PCR conditions are
95 for 5 minutes
95 for 30 seconds
52 for 45 seconds
68 for 6 minutes and 30 seconds
30 cycles of that
68 for 5 more minutes
Thanks for the correction about the restriction sites. I'll add some junk to the 5' end.
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Re: [DIYbio] PCR trouble
5:06 PM |
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