[DIYbio] low cost open electroporator

On 04/28/2016 02:00 AM, Patrik D'haeseleer wrote:
> Given how dirt cheap flyback transformers and other high voltage gear are on my favorite online discount site
> <http://www.goldmine-elec-products.com/products.asp?dept=1480>, perhaps that might be a better way to go.
>

I have a back burner project to develop that. There are so few asking for it though, so I work on another project
to generate income to have enough momentum to develop anything at all first.

On 04/28/2016 03:53 AM, Marc Juul wrote:
> Add an ESP8266 and you'd be able to connect via wifi+web to download and view the conductance measurement and discharge graph for
> use in your scientific article.
>

I'll be making a multi pulser flyback version so it can stretch a pulse, (with many peaks), out to 5ms or cook for 300 ms
if desired. And then the power flow and impedance can be adjusted for different conductivity test cells. I'm a backer of
the micropython on esp8266 and could easily use that one as a controller for this project. Are you going to commit time, and
will you license it open hardware compatible?

On 04/28/2016 03:12 PM, Nathan McCorkle wrote:
> Is electroporation preferable? From the first time I used it, my first
> summer internship, I was hooked. I thought "screw chemical
> transformation" for the, in my opinion, more elegant, tuneable,
> eco-friendly/greener-chemistry, faster, wider species coverage (you
> just vary the pulse shape/voltage, maybe the salt content of the
> buffer)... and higher efficiency to boot.
>
> Why isn't it more prominent? Because no one has made a cheaper or
> open-source version yet.

OK, so will you help me promote it with twitter, instagram, snap chat videos when the time comes?
Who to sell to besides DIYers?

There were some discussions I kept "best of" emails from before 2014 when my list server crashed
without a backup I can find anymore. We were talking with a retired Oak Ridge nuclear engineer
developing RF induction heaters using a car induction coil. It's all a little heavy and expensive compared
to the flyback parts used in laser printers -- I have a box full of those to test with...

The list has been up again since just after that crash if anyone wants to use it.
http://lists.cibolo.us/mailman/listinfo/open_electroporator
I think here on diybio@googlegroups.com is good for worthwhile
milestones, and there have not really been any. Not much was lost from that crash.
For saving old crusty details, there is anew list serve besides googlegroups by an original Yahoo.com
businessman called groups.io -- it will have ads.

John Griessen

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