Re: [DIYbio] low cost open electroporator

Now thinking back to those discussions, there was a lot of concern
with isolation. Would a wireless microcontroller obviate most/all
concerns? Obviously isolation would still be good to protect the
microcontroller... but assuming isolation development time cost more
than a few $2 replacement ESP8266... well, does that assumption hold
true?

On Fri, Apr 29, 2016 at 9:54 AM, John Griessen <john@industromatic.com> wrote:
> On 04/28/2016 02:00 AM, Patrik D'haeseleer wrote:
>> Given how dirt cheap flyback transformers and other high voltage gear are
>> on my favorite online discount site
>> <http://www.goldmine-elec-products.com/products.asp?dept=1480>, perhaps
>> that might be a better way to go.
>>
>
> I have a back burner project to develop that. There are so few asking for it
> though, so I work on another project
> to generate income to have enough momentum to develop anything at all first.
>
> On 04/28/2016 03:53 AM, Marc Juul wrote:
>> Add an ESP8266 and you'd be able to connect via wifi+web to download and
>> view the conductance measurement and discharge graph for
>> use in your scientific article.
>>
>
> I'll be making a multi pulser flyback version so it can stretch a pulse,
> (with many peaks), out to 5ms or cook for 300 ms
> if desired. And then the power flow and impedance can be adjusted for
> different conductivity test cells. I'm a backer of
> the micropython on esp8266 and could easily use that one as a controller for
> this project. Are you going to commit time, and
> will you license it open hardware compatible?
>
> On 04/28/2016 03:12 PM, Nathan McCorkle wrote:
>> Is electroporation preferable? From the first time I used it, my first
>> summer internship, I was hooked. I thought "screw chemical
>> transformation" for the, in my opinion, more elegant, tuneable,
>> eco-friendly/greener-chemistry, faster, wider species coverage (you
>> just vary the pulse shape/voltage, maybe the salt content of the
>> buffer)... and higher efficiency to boot.
>>
>> Why isn't it more prominent? Because no one has made a cheaper or
>> open-source version yet.
>
> OK, so will you help me promote it with twitter, instagram, snap chat videos
> when the time comes?
> Who to sell to besides DIYers?
>
> There were some discussions I kept "best of" emails from before 2014 when my
> list server crashed
> without a backup I can find anymore. We were talking with a retired Oak
> Ridge nuclear engineer
> developing RF induction heaters using a car induction coil. It's all a
> little heavy and expensive compared
> to the flyback parts used in laser printers -- I have a box full of those to
> test with...
>
> The list has been up again since just after that crash if anyone wants to
> use it.
> http://lists.cibolo.us/mailman/listinfo/open_electroporator
> I think here on diybio@googlegroups.com is good for worthwhile
> milestones, and there have not really been any. Not much was lost from that
> crash.
> For saving old crusty details, there is anew list serve besides googlegroups
> by an original Yahoo.com
> businessman called groups.io -- it will have ads.
>
> John Griessen
>
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--
-Nathan

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