In my experience, saliva swabs just work fine. You just need to avoid high temperatures and store at 4ºC (normal fridge) or lower.
-- https://www.amazon.com/Whatman-WB100035-Omni-Buccal-Sterile/dp/B00394FIQA
I bought cheaper, just take a look for Omniswab (general electric)
If you can, avoid Oragene and similars. They give you nice amount of quality DNAm, but the chepeast is about 15USD/unit.
In order to extract the DNA from the cells, I would recommend some cheap brand like Tiangen or similar (aliexpress/ebay). You just need a centrifuge and a micropipiete.
So, you can send the swab with a small zip bag. Then, the user collects the sample and send it back to you. I know about contamination problems blablabla... but it works
El lunes, 20 de junio de 2016, 15:49:24 (UTC+2), Bryan Daniels escribió:
I am looking for a nice manageable project to do with my kids (ages 12 - 16). I've decided to use a kit (http://www.minipcr.com/product/minipcr-genotype-to- ). Nice example of Mendelian inheritance. Compare genotype with phenotype and see if it actually works in practicephenotype-ptc-taster-lab/ I would like to do this not just for immediate family, but also for extended family. (All in US.) Any suggestions on how to get check samples and ship them back to us for analysis. I have seen some rather expensive commercial kits, but wonder about a DIY approach to this. I had intended to send each relative PTC paper, swab, vial for completed samples and a return envelopevpacks. Not sure about preserving DNA and shipping regulations. Any thoughts or advice would be appreciated.
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/3f997c11-f918-4be5-ad2e-408e8e88817d%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.
0 comments:
Post a Comment