Re: [DIYbio] Capillary electrophoresis that I made but can be improved

Hi Gordana,

Thank you for your comments.

I feel more encouraged to start thinking about a capillary electrophoresis for analysis of water quality.

Best Regards,
Markos

Hi Markos,

I initially started with this HV - 2KV and that one discharges slowly. I got myself shocked couple of times before I made a parallel connection with a switch and a light bulb to discharge it. I thought that one can put in-line fuse with some limiting current to prevent large current going through you or equipment, or in my case I used batteries to supply input voltage (that limits how much energy it can discharge so I felt safer). Also, you can discharge it by touching electrodes but it does produces awful spark and it is not good in the long term for electrodes or circuits around. 

After I burned this one (left the discharge circuit on while it was working so the transistor on the board overheated), I got the HV -7 and 20 KV.    These are step up type (not sure about the previous one) and I think they should be good for CE since one does not require lots of current. They are also safer since overall energy is small. These discharge fast so I removed parallel discharge circuit. 

Regarding the measurements of polarity and voltage, they all have labeled input (red for +) and white for -, and output I checked with 15 1MOhms resistors and 0.2 MOhms  in series, and measured voltage on 200 KOhm  to check the polarity of electrodes. 

Faraday cage is to protect external electronics from the electric field. Detection circuit will register hand waving above it so that should also be Faraday cage-d but I just stay away during the measurements. If you are afraid of HV I suggest to buy smaller voltage one and play a bit. 

Cheers!  

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/417ace24-6d6c-4482-a163-6a6cc7d69f4d%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.