Re: [DIYbio] Using isopropanol in place of ethanol in DNA purification kits?

From the mini-prep folks:
https://www.qiagen.com/us/resources/molecular-biology-methods/dna/#Isopropanol
precipitation of DNA

Mentions better precip at warmer temps, better salt solubility (which
leads to harder to see glassy pellets rather than clouded-with-salt
pellets)... but the main point I remember: downstream processes could
be affected -- because isopropanol is less volatile it dries more
slowly or not completely if you are too fast... slower drying gives
more time for energy-state minimization, thus when it is dry it is
harder to redissolve (it must be denser and more closely-packed I
guess).

On Thu, Jul 21, 2016 at 5:07 PM, Cathal (Phone)
<cathalgarvey@cathalgarvey.me> wrote:
> Isoprop is fine for washing minipreps. IIRC it has slightly different
> performance on RNA, and it will behave differently WRT salt also. But, for
> minipreps, it's not very important. You need a bit less of it than ethanol,
> it's a stronger precipitant.
>
> Warning: DNA pellets washed with ethanol tend to become white and easily
> visible, whereas with isoprop they can be glassy and transparent.
>
> On 22 July 2016 00:31:13 IST, Cory Tobin <cory.tobin@gmail.com> wrote:
>>
>> I've used isopropanol for Qiagen minipreps before and it didn't make
>> any noticeable difference.
>>
>> -cory
>
>
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--
-Nathan

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