Isoprop is fine for washing minipreps. IIRC it has slightly different performance on RNA, and it will behave differently WRT salt also. But, for minipreps, it's not very important. You need a bit less of it than ethanol, it's a stronger precipitant.
Warning: DNA pellets washed with ethanol tend to become white and easily visible, whereas with isoprop they can be glassy and transparent.
On 22 July 2016 00:31:13 IST, Cory Tobin <cory.tobin@gmail.com> wrote:
I've used isopropanol for Qiagen minipreps before and it didn't make
any noticeable difference.
-cory
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Sent from my Android device with K-9 Mail. Please excuse my brevity.
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