I haven't tried with the PEG. I recently did another side by side comparison with SLICE made from DH5a cells without pkD46, with pkD46 uninduced and with pkD46 induced. This was just a single shot, so not significant results, but the pkD46 made a big difference, but it didn't matter a much whether it was induced or not. Both SLiCE mixes made with pkD46 gave me about half as many colonies as Gibson Assembly. The reaction was a single gene insert into a plasmid (~1kb and ~5kb) with 25bp overlap on either end.
On Thu, Oct 20, 2016 at 2:36 AM Cathal Garvey <cathalgarvey@cathalgarvey.me> wrote:
Very fair point, yes! This is outside my comfort zone, in terms of calculating the impacts. It's an observation I've made that many protocols allow substitution, but honestly I can't remember whether they specify different molarities.It would seem to me that yes; longer polymers would mean more soupy buffers. But the difference between PEG50 and PEG3350 is probably orders of magnitude larger than the difference between PEG3350 and PEG8000?Anyone know good back-of-envelope ways to calculate the relative effects of polymers by chain length? :)--
On Wed, Oct 19, 2016 at 7:21 PM, John Griessen <john@industromatic.com> wrote:
On 10/19/2016 12:55 PM, Cathal Garvey wrote:Just an FYI: you can often substitute PEG3350 for PEG8000 in protocols. Just watch for molarity, substituting it gram-for-gram is probably unwise.Isn't the number after the PEG acronym for length or molecular weight? Its property of interest is absorbing/adsorbing water, so the much bigger molecule at the same number of moles would turn a broth into a pudding. Right?---- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en Learn more at www.diybio.org --- You received this message because you are subscribed to the Google Groups "DIYbio" group. To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com. To post to this group, send email to diybio@googlegroups.com. Visit this group at https://groups.google.com/group/diybio. To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/7f7b1f9e-1cab-b5f5-5b43-25a9d460e1d7%40industromatic.com. For more options, visit https://groups.google.com/d/optout.
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