I've been looking into in-vitro ttranscription. It looks like it could avoid GM regulation issues and be fine for some projects. These kits' prizes are horrible though. Does anyone know where to get the cheapest ones?
Also I was thinking about unifying the process. Nowadays you do:
T7-> RNA synthesis
5' capping
polyA tail addition
There are some viral RNA elements which substitute 5' cap and polyA tail, so maybe it would be xool to have a plasmid with this elements. All you need is to add T7 polymerase, and you get stable mRNA that can be translated (in vivo or in vitro). Any thoughts on that?
-- Also I was thinking about unifying the process. Nowadays you do:
T7-> RNA synthesis
5' capping
polyA tail addition
There are some viral RNA elements which substitute 5' cap and polyA tail, so maybe it would be xool to have a plasmid with this elements. All you need is to add T7 polymerase, and you get stable mRNA that can be translated (in vivo or in vitro). Any thoughts on that?
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