hello everyone
-- i am currently working on a project for production of vaccine based on stable transformation of chlorella vulgaris. the plasmid size is around 6 Kb and i wanted to transformed into chlorella vulgaris using electroporation to get a stable expression of my protein of interest and also i wanna analyze the site of integration into genome. we have bio-rad gene pulser II in our lab.
so can someone help me for the design of protocol for the transformation.
thanks in advance.
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