Hello Najib,
This reference might help you with designing your electroporation experiment. You will need to linearize your vector for stable integration.
-- This reference might help you with designing your electroporation experiment. You will need to linearize your vector for stable integration.
https://link.springer.com/article/10.1007/s002990050660
There are many protocols for looking at transgene integration sites, inverse PCR being the most popular. There are even kits but I'm old school.
https://www.ncbi.nlm.nih.gov/pubmed/9893713
https://genome.cshlp.org/content/1/2/129.full.pdf
Compare your integration sequences with the Chlorella vulgaris assembly.
https://www.ncbi.nlm.nih.gov/assembly/GCA_001021125.1/
Hope this helps and all the best with your experiments.
Cheers,
Scott
https://opensciencenet.org
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