Chances are that when you go to isolate the genetic material and look for your insertion site, there will be plenty of remaining vector. I'm not sure what method you were going to use to determine this (qPCR, gel validation, sequencing, ddPCR, etc.) but here is an interesting paper about how Eukaryotic cells retain their vectors for quite a while.
I hope this helps
-SG
On Wed, Jul 11, 2018 at 12:01 PM, Scott <synbiofablab@gmail.com> wrote:
Hello Najib,--
This reference might help you with designing your electroporation experiment. You will need to linearize your vector for stable integration.There are many protocols for looking at transgene integration sites, inverse PCR being the most popular. There are even kits but I'm old school.Compare your integration sequences with the Chlorella vulgaris assembly.Hope this helps and all the best with your experiments.Cheers,Scott
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com . For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com .
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio .
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/4612829d-d86d- .4aa3-aec7-8239f187d9ea% 40googlegroups.com
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CAGA%2B4LUWG_6HeWEuFHvuru0UyXkOVzpL_Atho84S-eq4NZAh-w%40mail.gmail.com.
For more options, visit https://groups.google.com/d/optout.
0 comments:
Post a Comment