Have you worked with RNA before? Do you have a sample which you have used before to extract RNA as a positive control?
-- Is your work station clean? Do you wear gloves? Do you use filter tips on your pipets? RNAse is everywhere. A smear at the bottom the gel suggests degraded RNA.
On Wednesday, January 9, 2019 at 5:13:29 AM UTC-8, najib abdellaoui wrote:
On Wednesday, January 9, 2019 at 5:13:29 AM UTC-8, najib abdellaoui wrote:
hello everyonei am working on chlorella vulgaris and trying to extract RNA from it using different methods:
- i tried to use liquid nitrogen combined with trizol,
- i tried to use plant kit from invitrogen,
however, i couldn't get good results when i run the RNA sample on gel. i couldn't get the 28 and 18S bands. i got just a smear at the bottom of the gel.can someone help me for the rna extractionthank you.
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