before using the work station, i clean it, i wear gloves and worked with RNA before using animal cells and fish organs. for the positive control, in the lab they didn't extract RNA before, so there is no positive control for RNA. for the filter tips, we don't have them recuse of budget limitation.
On Thursday, January 10, 2019 at 1:24:47 AM UTC+9, John Ladasky wrote:
-- On Thursday, January 10, 2019 at 1:24:47 AM UTC+9, John Ladasky wrote:
Have you worked with RNA before? Do you have a sample which you have used before to extract RNA as a positive control?Is your work station clean? Do you wear gloves? Do you use filter tips on your pipets? RNAse is everywhere. A smear at the bottom the gel suggests degraded RNA.
On Wednesday, January 9, 2019 at 5:13:29 AM UTC-8, najib abdellaoui wrote:hello everyonei am working on chlorella vulgaris and trying to extract RNA from it using different methods:
- i tried to use liquid nitrogen combined with trizol,
- i tried to use plant kit from invitrogen,
however, i couldn't get good results when i run the RNA sample on gel. i couldn't get the 28 and 18S bands. i got just a smear at the bottom of the gel.can someone help me for the rna extractionthank you.
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