I'd recommend that you unplug the capacitance extender and pulse controller and put them in the closet until you think you need them. The base unit is fine for almost all work. If you are doing bacterial samples, aim for a time constant of 5 ms with samples loaded. The base unit puts a 200 ohm resistor in parallel with the sample chamber, so you can try this out with no sample loaded. Adjust the capacitor to give a 5 ms pulse. If you samples are high resistance, you should not need to change anything. For E. coli, try 1.8 KV with a 2 mm cuvette, possibly up to 2.2 KV. For 1 mm cuvette, try 1.2 KV or so.
On Mar 15, 2020, at 3:46 PM, Tom De Medts <tdmedts@gmail.com> wrote:Hi folks,
The BIO Rad gene Pulser I electroporator arrived from Ebay today.Can someone share a wiring diagram (even if hand drawn) for a setup containing the modules of the BIO RAD Gene Pulser I Electroporator, consisting of
- Gene Pulser (bottom most)
- Pulse Modulator (mid tier)
- Capacitance Extender (top most module)
as shown from 4 annotated pics below of the 4 different sides of the stacked modules, please?I am not entirely sure how the wires from each module goes to the next one, hence this request and the accompanying pics. Thank you, in advance.Do I even need the pulse modulator and the capacitance extender, if all that I intend to use this for, is electoporation of E. coli or Agrobacterium?Cheers,TomPS. You should be able to download and zoom into the pics, if you wish. Or open in a new tab, and expand...<Front_setup.jpg> <Right_setup.jpg> <Back_setup.jpg> <Left_setup.jpg>On Tue, Mar 10, 2020 at 2:36 PM Nathan McCorkle <nmz787@gmail.com> wrote:Please note that electronic lighter piezoelectric elements can vary significantly in voltage output, by at least 10x depending on a particular given lighter source. It just means you need more testing to classify a piezo element before one of those lighter based circuits will work effectively.--
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