in layman's terms, would be great to have the means to harvest and prepare adipose derived stem cells for reinjection.
------ Original Message ------
From: "Patrik D'haeseleer" <patrikd@gmail.com>
To: "DIYbio" <diybio@googlegroups.com>
Sent: 6/28/2020 3:51:37 AM
Subject: [DIYbio] Re: Establishing a New Haven Community Bio Space - Equipment List
$20K is a real luxury, but please don't blow it all on buying brand new equipment if you can get functionally the same gear for as little as a tenth of the price second hand on eBay, CraigsList, or auction sites.--- Definitely do yourself a favor and get a real biosafety cabinet instead of the laminar flow- The incubator you have listed seems tiny. Get an undercounter one. You might need to store 50 or more plates if you want to run Agar Art workshops. Maybe get the tiny one as well, in case you need to incubate at two different temperatures - e.g. yeast vs E. coli- No need for a plasmapheresis machine - you should not be working with blood in a BSL-1 lab.- That Formlabs printer is 1/3 of your entire budget - are you really, really sure you need an expensive high-end resin printer like this? As someone who helps run the bioprinter group at BioCurious, I wouldn't dream of getting this one, unless you knew *exactly* how you will be using it and why it needs to be this particular model, and you're planning for this to be a major feature of what you do at your community lab. If you just want a resin printer, there are several good ones under $1000 now.- A large centrifuge like that can be quite dangerous. Also not really that useful unless you start growing large volumes of cells, but then you should invest in bioreactors as well. You will likely get a lot more mileage out of the microcentrifuge.- Need a -20 lab freezer as well (NOT a kitchen freezer - don't want that auto-defrost feature). Undercounter fridge and freezer can typically be had second hand for $100-200 each.Best of luck! And remember, this is a marathon, not a sprint...Patrik
On Saturday, June 27, 2020 at 1:26:32 PM UTC-7, J.R. Logan wrote:We have been very fortunate to get a state innovation grant approved to fund the establishment of a community/DIY bio space in our makerspace. Informed by the various online equipment lists and my own understanding from talking to bio space organizers at the Community Bio Summit I came up with a proposal for a proposal of $21,700 ($6,000 of Infrastructure, $2,000 of Program expense and $13,700 of Equipment). In the proposal I noted it as a minimum viable budget, and a more full featured lab would cost at another $20,000 or more. The funding committee not only approved the plan but asked to see the list of equipment we would acquire if we received the additional $20,000.To meet their request I have compiled a list of items and general expected expenses. The items on the list are representative and we will research the best we can get for the price as we order specific models. I know the specific expenses will work out somewhat differently but that is OK with this funding as long as the overall total equipment expense is close.I would appreciate it if anyone with experience running a community bio lab would review and comment on my list and proposal. What are my blind spots on this? Anything really important I am missing? Are there certain items where I should buy the best we can afford? Others where it is wasteful to buy, as getting donations is common? If you think it looks good, let me know. It will be helpful to tell the review committee I have validated my proposal with the wider community of bio space leaders.
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