Re: [DIYbio] Start up

1:29 PM |

In my experience, most pipette tips do not autoclave well - since the tips are generally compression-fit onto the pipette, even slight deformations on the plastic lead to inaccuracies in pipetting performance.

My suggestion would be to buy a cheap sonicator off eBay and do as follows:
1) pre-soak your tips for 20-30 mins in 10% bleach (which will kill any living organisms, and thoroughly oxidize both nucleic acids and proteins)
2) rinse in water a couple of times (it's important to get rid of all the bleach, which will otherwise interfere with downstream applications)
3) sonicate your tips for 10-20 mins in clean water
4) do a final rinse in 70% IPA / EtOH, rack while wet and dry under HEPA-filtered air flow (a standard laminar hood will do the trick)

If you have access to a 3D printer, this tool will make your life much easier: https://www.labonthecheap.com/3d-print-a-pipette-tip-sorter/

On Tue, Mar 23, 2021 at 4:53 AM 'Cathal Garvey' via DIYbio <diybio@googlegroups.com> wrote:
In this vein, using a dilute peroxide-based bleaching agent such as sodium percarbonate might be a nice pre-wash before autoclaving. Peroxide bleaching (followed by rinsing with cheap car-grade deionised water) will go a long way toward decontaminating and removing residues, and the peroxide will decompose entirely within the autoclave whereas chlorine bleaches might leave residues.

The deionising rinse may be necessary though because sodium percarbonate would perhaps otherwise leave a minor salt residue that could mess with some molecular work. 

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23 Mar 2021, 10:27 by sjamesparsonsjr@gmail.com:
Bleach is cheap, just make sure the tips don't melt 

Sent from my iPhone

On Mar 23, 2021, at 3:05 AM, Frank <fgarcia0007@gmail.com> wrote:

is the radiation  in a microwave oven not sufficient to sterilize?

------ Original Message ------
From: "M Marcus" <maghimaimarcus@gmail.com>
Sent: 3/23/2021 2:13:24 AM
Subject: Re: [DIYbio] Start up

Do you have pressure cooker at home. It's internal temperature reaches 121 deg celsius when it reaches the first whistle. So 20 minutes of those items  inside a pressure cooker is autoclaving. It costs around 150 dollars. Or you can loan  a used one from one of your indian friends for free . 

On Tue, Mar 23, 2021 at 10:52 AM S. Almo <suha.almohammadi@gmail.com> wrote:
Hello

I want to be able to reuse my tips, tubes and needles. as i am mainly using those to train for biological techniques at home using my bento lab. i do not have the money to buy more tips and tubes. is there a way i can autoclave at home or just deep wash them.

Any suggestions?


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Best
M Marcus
(91) 9884090469
Chennai


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