A pressure cooker may not be ideal for this situation because of the tight tolerances of pipette tips, but they do work very well as a home autoclave.
However, it's important to note that not all autoclaves are calibrated to the same pressure. That means they will reach different internal temperatures. I don't have the formulas in front of me but it should be pretty easy to calculate the max internal temperature based on the pressure your equipment can reach.
Also, someone correct me if I'm wrong, but I believe that you don't need to worry about altitude with a pressure cooker. Normally water would boil at a lower temperature as you go up in altitude. But pressure cookers work by setting a calibrated weight on top of the steam vent. That should eliminate the impact of atmospheric pressure, so us mountain dwellers don't need to worry that we're not getting adequate sterilization.
Again, please correct me if I'm wrong on that, I've never taken a physics class in my life.
Cheers,
Dan
On Tue, Mar 23, 2021, 12:42 AM M Marcus <maghimaimarcus@gmail.com> wrote:
Do you have pressure cooker at home. It's internal temperature reaches 121 deg celsius when it reaches the first whistle. So 20 minutes of those items inside a pressure cooker is autoclaving. It costs around 150 dollars. Or you can loan a used one from one of your indian friends for free .On Tue, Mar 23, 2021 at 10:52 AM S. Almo <suha.almohammadi@gmail.com> wrote:HelloI want to be able to reuse my tips, tubes and needles. as i am mainly using those to train for biological techniques at home using my bento lab. i do not have the money to buy more tips and tubes. is there a way i can autoclave at home or just deep wash them.Any suggestions?--
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