Re: [DIYbio] TN Plant TC project

Sounds very interesting...

As I am sure you know, Plant Tissue Culture (PTC), due to prolonged growth of plant material in media containing sugars, is very sensitive to contamination issues.  Therefore, I'm curious to know how you have dealt with it or plan to.

I built my own laminar flow, it was neither very easy nor inexpensive. However it worked out to be way cheaper than buying a class I or II hood.

And way simpler than buying one, shipping on a pallet, which would more often than not require a formally registered entity to buy shipping insurance and a formal delivery address that is not residential.

Other issues I could discuss, but I see the availability of a proper sterile working area as one of the major stumbling blocks for anyone interested in PTC.

So I'll wait for your response to this concern first....

And apart from your technical setup, there's the question about whether micropropagation of wild ginseng 

has a real and practical runway leading to sustainable rural development. Who are your identified partners,

and what synergy do you have with them? My PTC goals are commercial, so I don't think on these lines,

and therefore it's possible I may not even be able to relate to some aspects of your go-to-market strategy...

On Sat, Apr 3, 2021 at 9:55 PM trent smith <tnot...@gmail.com> wrote:

I've been researching a project thats ready as ever to go.  It's a social innovation/sustainable development adventure.  Micropropagation of wild local ginseng to engage public, private, academic community in processes and practices with the objective to transition to sustainable rural development.  I've started pulling together lab equipment. I've got the idea and know just enough about TC protocol to be dangerous.  Hit me up if your in Middle Tennessee and interested or if you have advice.

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