Re: Re[4]: [DIYbio] Substitute for calf serum?

Thanks Bryan,

I had just come across that paper. Very useful. Seems like the thing to use is platelet lysate from my own blood.

------ Original Message ------

From: "Bryan Jones" <bryanjjones@gmail.com>

To: "DIYbio" <diybio@googlegroups.com>

Sent: 4/14/2021 11:44:07 AM

Subject: Re: Re[2]: [DIYbio] Substitute for calf serum?

Here's a pretty good summary article on different alternatives to FBS. https://pharma.lonza.com/-/media/Lonza/knowledge/CGT/A_consensus_introduction_to_serum_replacements_CGT.pdf

There is also a database of articles that have reported non-FBS growth media for various cell types: https://fcs-free.org/fcs-database

I think what S James Parsons is talking about is a defined media (where each protein, growth-factor, salt, and other component is explicitly defined) . There are companies that make defined media, but I'm not sure if any are as good or affordable as FBS-based media. It is very desirable to use something defined and animal-free for any GMP product or product to be used in the clinic.

--Bryan Jones

On Mon, Apr 12, 2021 at 1:20 PM Frank <fgarcia0007@gmail.com> wrote:

I did a little more reading https://www.frontiersin.org/articles/10.3389/fcell.2016.00115/full and it turns out that human serum works even better than calf serum. derivatives of this that are  serum, plasma, platelet rich plasma, platelet poor plasma, and platelet lysate. I can easily make these myself from my own blood for the price of blood collection tubes or $0 with zero cruelty compared to $1000 for a liter of FBS which is obtained in a hideously cruel way. This article and others ouitline the many advantages to abandoning calf serum.

Yay!

------ Original Message ------

From: "S James Parsons Jr" <sjamesparsonsjr@gmail.com>

To: diybio@googlegroups.com

Sent: 4/12/2021 10:09:31 AM

Subject: Re: [DIYbio] Substitute for calf serum?

Human serum would work, the problem is cost and amount of material required.  

My idea is to create a profile of FBS, salt, proteins, the the like. Then find the minimum of each protein, salt, and growth factor needed for tissue culturing.   Once identified, create a transmutated organism (yeast, bacteria) to produce said proteins.  Then use that elixir as a replacement for FBS.

Boom, free hamburgers for life! 

On Apr 12, 2021, at 12:33 AM, Frank <fgarcia0007@gmail.com> wrote:

Thanks James or responding to my question. Your answer made me curious as I find it hard to believe that there could only be one product on the market for anything .  I found what appears to be a bunch of products marketed as substitutes for calf serum with some interesting advantages. Here are a couple. What do you think of these? Are they calf serum alternatives

Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media.  C Rauch, E Feifel, EM Amann, HP Spötl… - ALTEX-Alternatives to …, 2011 - altex.org

Human autologous serum obtained using a completely closed bag system as a substitute for foetal calf serum in human mesenchymal stem cell cultures.  N Mizuno, H Shiba, Y Ozeki, Y Mouri… - Cell biology …, 2006 - Wiley Online Library

… AB serum and thrombin‐activated platelet‐rich plasma are suitable alternatives to fetal calf serum for the expansion of mesenchymal stem cells from adipose tissue.  A Kocaoemer, S Kern, H Klüter, K Bieback - Stem cells, 2007 - Wiley Online Library

 

------ Original Message ------

From: "S James Parsons Jr" <sjamesparsonsjr@gmail.com>

To: diybio@googlegroups.com

Sent: 4/11/2021 3:07:16 PM

Subject: Re: [DIYbio] Substitute for calf serum?

That is the holy grail of tissue culturing.  If you find a magic elixir with growth hormones, salts, proteins, enzymes, and a desired pH you would be a wealthy person! 

On Apr 11, 2021, at 2:52 PM, Frank <fgarcia0007@gmail.com> wrote:

Can anyone please tell me if I can use a substitute when a cell culturing protocol calls for calf serum? specifically, a substitute that does not involve cruelty to animals. is it possible to use my own blood serum?

-- 
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
--- 
You received this message because you are subscribed to a topic in the Google Groups "DIYbio" group.
To unsubscribe from this topic, visit https://groups.google.com/d/topic/diybio/qVcVf5ZnPp4/unsubscribe.
To unsubscribe from this group and all its topics, send an email to diybio+unsubscribe@googlegroups.com.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/732C4289-C0F5-47FF-B3B5-B6FE01B23FBA%40gmail.com.

-- 
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
--- 
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/em9297f14b-d05a-408b-96c6-2248711c6451%40cabron.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/7CF94DB7-D99D-48C9-8F2F-531710F01FAA%40gmail.com.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/em1da3954a-aceb-4bca-8410-9b3b3adeb3bc%40cabron.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CAKw3Q70OmLKu0AR1mhAi4S%3Da%2BJQUmgcKD_udYPXaMOHhROzxUg%40mail.gmail.com.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/em8a8003d4-e20f-46c7-967e-510835107551%40cabron.