Re[4]: [DIYbio] Substitute for calf serum?

1:24 PM |

Thanks Bryan,
I had just come across that paper. Very useful. Seems like the thing to use is platelet lysate from my own blood.

------ Original Message ------
From: "Bryan Jones" <bryanjjones@gmail.com>
Sent: 4/14/2021 11:44:07 AM
Subject: Re: Re[2]: [DIYbio] Substitute for calf serum?

Here's a pretty good summary article on different alternatives to FBS. https://pharma.lonza.com/-/media/Lonza/knowledge/CGT/A_consensus_introduction_to_serum_replacements_CGT.pdf
There is also a database of articles that have reported non-FBS growth media for various cell types: https://fcs-free.org/fcs-database
I think what S James Parsons is talking about is a defined media (where each protein, growth-factor, salt, and other component is explicitly defined) . There are companies that make defined media, but I'm not sure if any are as good or affordable as FBS-based media. It is very desirable to use something defined and animal-free for any GMP product or product to be used in the clinic.
--Bryan Jones


On Mon, Apr 12, 2021 at 1:20 PM Frank <fgarcia0007@gmail.com> wrote:
I did a little more reading https://www.frontiersin.org/articles/10.3389/fcell.2016.00115/full and it turns out that human serum works even better than calf serum. derivatives of this that are  serum, plasma, platelet rich plasma, platelet poor plasma, and platelet lysate. I can easily make these myself from my own blood for the price of blood collection tubes or $0 with zero cruelty compared to $1000 for a liter of FBS which is obtained in a hideously cruel way. This article and others ouitline the many advantages to abandoning calf serum.

Yay!


------ Original Message ------
From: "S James Parsons Jr" <sjamesparsonsjr@gmail.com>
Sent: 4/12/2021 10:09:31 AM
Subject: Re: [DIYbio] Substitute for calf serum?

Human serum would work, the problem is cost and amount of material required.  

My idea is to create a profile of FBS, salt, proteins, the the like. Then find the minimum of each protein, salt, and growth factor needed for tissue culturing.   Once identified, create a transmutated organism (yeast, bacteria) to produce said proteins.  Then use that elixir as a replacement for FBS.

Boom, free hamburgers for life! 




On Apr 12, 2021, at 12:33 AM, Frank <fgarcia0007@gmail.com> wrote:

Thanks James or responding to my question. Your answer made me curious as I find it hard to believe that there could only be one product on the market for anything .  I found what appears to be a bunch of products marketed as substitutes for calf serum with some interesting advantages. Here are a couple. What do you think of these? Are they calf serum alternatives

Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media.  C Rauch, E Feifel, EM Amann, HP Spötl… - ALTEX-Alternatives to …, 2011 - altex.org

 

------ Original Message ------
From: "S James Parsons Jr" <sjamesparsonsjr@gmail.com>
Sent: 4/11/2021 3:07:16 PM
Subject: Re: [DIYbio] Substitute for calf serum?

That is the holy grail of tissue culturing.  If you find a magic elixir with growth hormones, salts, proteins, enzymes, and a desired pH you would be a wealthy person! 

On Apr 11, 2021, at 2:52 PM, Frank <fgarcia0007@gmail.com> wrote:

Can anyone please tell me if I can use a substitute when a cell culturing protocol calls for calf serum? specifically, a substitute that does not involve cruelty to animals. is it possible to use my own blood serum?


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