Re: [DIYbio] 0.2 um filters for sterile filtering/possibly viscous solutions

I called these folks last week looking for filters, they're pretty much the industry standard for this sort of thing in biotech, they were very helpful to me so maybe you can give a call and ask for higher volume items


On Mon, Dec 31, 2012 at 6:36 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
the pore size is what's going to determine the pressure requirement for forcing the fluid past the membrane, so you could just parallelize with the smaller filters if you can't find a larger diameter one.


On Mon, Dec 31, 2012 at 8:43 AM, Eugen Leitl <eugen@leitl.org> wrote:

Happy $holidays!

A quick question: I need to sterile-filter several 10 l of a
reasonably viscous solution into autoclavable borosilicate
lab bottles.

This is typically done with syringe filters,

http://www.gilsonuk.com/catalogue/product/itemNo/ALG422A

but that's on a too small scale, and for rather nonviscous
solutions.

I was thinking about repurposing household items like
http://www.amazon.de/Keramikfilter-Wasserfilter-NEU-Filter-Feinfilter/dp/B002RLKE7O/
or http://www.amazon.de/Polypropylene-Auswaschbar-UMKEHROSMOSE-WASSERFILTER-SEDIMENTFILTER/dp/B002RLZS7U/

Has any of you done that? How did you sterilize the assembly?
(Polypropylene is obviously only cold-sterilizable, and
I suspect the plastic parts of the ceramics one won't
survive autoclaving either).

At this point I'm thinking about overnight soaking the ceramics
filter/whole filtering assembly in hydrogen carbonate/hydrogen
peroxide, 70% isopropanol or bleach, and flushing out the assembly
for an hour with reverse osmosis water, possibly prefiltered.

Thanks and a nice 2013 to ye all.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
For more options, visit https://groups.google.com/groups/opt_out.





--
-Nathan



--
-Nathan

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
For more options, visit https://groups.google.com/groups/opt_out.
 
 

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

Re: [DIYbio] 0.2 um filters for sterile filtering/possibly viscous solutions

the pore size is what's going to determine the pressure requirement for forcing the fluid past the membrane, so you could just parallelize with the smaller filters if you can't find a larger diameter one.


On Mon, Dec 31, 2012 at 8:43 AM, Eugen Leitl <eugen@leitl.org> wrote:

Happy $holidays!

A quick question: I need to sterile-filter several 10 l of a
reasonably viscous solution into autoclavable borosilicate
lab bottles.

This is typically done with syringe filters,

http://www.gilsonuk.com/catalogue/product/itemNo/ALG422A

but that's on a too small scale, and for rather nonviscous
solutions.

I was thinking about repurposing household items like
http://www.amazon.de/Keramikfilter-Wasserfilter-NEU-Filter-Feinfilter/dp/B002RLKE7O/
or http://www.amazon.de/Polypropylene-Auswaschbar-UMKEHROSMOSE-WASSERFILTER-SEDIMENTFILTER/dp/B002RLZS7U/

Has any of you done that? How did you sterilize the assembly?
(Polypropylene is obviously only cold-sterilizable, and
I suspect the plastic parts of the ceramics one won't
survive autoclaving either).

At this point I'm thinking about overnight soaking the ceramics
filter/whole filtering assembly in hydrogen carbonate/hydrogen
peroxide, 70% isopropanol or bleach, and flushing out the assembly
for an hour with reverse osmosis water, possibly prefiltered.

Thanks and a nice 2013 to ye all.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
For more options, visit https://groups.google.com/groups/opt_out.





--
-Nathan

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
For more options, visit https://groups.google.com/groups/opt_out.
 
 

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

Re: [DIYbio] simple PCR methods

pretty much what lava-amp was going for, there was a lot of talk about this sort of thing a few years ago but it never picked up mainly because they were all focused on using circular capillaries that were either expensive/hard-to-get or weird/hard to work with (someone mentioned in a recent thread regarding annoying capillaries for an air thermocycler)

http://www.lava-amp.com/

On Mon, Dec 31, 2012 at 2:33 PM, John Griessen <john@industromatic.com> wrote:

http://www.biotechniques.com/news/The-ups-and-downs-of-convection-PCR/biotechniques-307800.html


They say, "As the sample entered the cool zone at the top of the tube, it underwent annealing and extension. Then, the DNA dropped to the bottom of the tube and was heated again. "

I think they are saying DNA expands to the point of being suspended when hot,
and sinks when cooled, or are they just saying some toroidal flow patterns happen
in the size tube they used?

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
--- You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
For more options, visit https://groups.google.com/groups/opt_out.





--
-Nathan

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
For more options, visit https://groups.google.com/groups/opt_out.
 
 

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

Re: [DIYbio] 0.2 um filters for sterile filtering/possibly viscous solutions

Something like this?
http://vgdusa.com/pall-capsule-filters.htm
Cheers
Brian

On Monday, December 31, 2012, Avery louie <inactive.e@gmail.com> wrote:
> They also make filters to go on bottles:
>
> http://fishersci.com/ecomm/servlet/fsproductdetail_10652_12704331_29104_-1_0?LBCID=26030796
>
> --A
>
> On Mon, Dec 31, 2012 at 12:59 PM, Eugen Leitl <eugen@leitl.org> wrote:
>>
>> On Mon, Dec 31, 2012 at 12:49:17PM -0500, Avery louie wrote:
>> > It might be possible to get pre sterilized filtration bottles.  Nalgene
>> > makes them pretty cheap.  No muss, no fuss, and they are vacuum assisted!
>>
>> We'll just flush
>> http://invitroshop.de/shop/laborbedarf/laborglas/laborflasche-simax-371.html
>> with ultrafiltered reverse osmosis water, and autoclave these prior
>> of sterile-filtering into them, then autoclave them prior to
>> fastening the cap.
>>
>> This is labor-intensive, but the result should have some pretty
>> good shelf half life.
>>
>>
>> --
>> -- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
>> Learn more at www.diybio.org
>> ---
>> You received this message because you are subscribed to the Google Groups "DIYbio" group.
>> To post to this group, send email to diybio@googlegroups.com.
>> To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
>> Visit this group at http://groups.google.com/group/diybio?hl=en.
>> For more options, visit https://groups.google.com/groups/opt_out.
>>
>>
>
> --
> -- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
> Learn more at www.diybio.org
> ---
> You received this message because you are subscribed to the Google Groups "DIYbio" group.
> To post to this group, send email to diybio@googlegroups.com.
> To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
> Visit this group at http://groups.google.com/group/diybio?hl=en.
> For more options, visit https://groups.google.com/groups/opt_out.
>  
>  
>

--
----------------------------------------
Brian Degger

twitter: @drbrian

----------------------------------------

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
For more options, visit https://groups.google.com/groups/opt_out.
 
 

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

[DIYbio] simple PCR methods

> http://www.biotechniques.com/news/The-ups-and-downs-of-convection-PCR/biotechniques-307800.html


They say, "As the sample entered the cool zone at the top of the tube, it underwent annealing and extension. Then, the DNA dropped
to the bottom of the tube and was heated again. "

I think they are saying DNA expands to the point of being suspended when hot,
and sinks when cooled, or are they just saying some toroidal flow patterns happen
in the size tube they used?

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
For more options, visit https://groups.google.com/groups/opt_out.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

Re: [DIYbio] First full DNA extraction, PCR, and gel

Found the old handout I had seen months ago!

http://dna.utah.edu/References/PDF_Public/RapidCyclist_V3_1995.pdf

In that one you can see the capillaries look like melting point ones,
and involve drawing it up with capillary action then flame sealing and
removing with that syringe thing I was talking about.

Those glass ones you posted look pretty nice, would probably improve
the efficiency with the setup I have now by ditching the thin walled
regular plastic PCR tubes.

http://www.biotechniques.com/news/The-ups-and-downs-of-convection-PCR/biotechniques-307800.html

That link applies to the ones you linked, they look identical.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
For more options, visit https://groups.google.com/groups/opt_out.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

Re: [DIYbio] First full DNA extraction, PCR, and gel

On 12/31/2012 12:49 PM, Dakota wrote:
> Yup heat transfer is the key, but you can avoid mineral oil with glass capillaries since you flame seal them into a closed
> environment. Getting the samples out of the capillaries requires some weird aspirator needle thing though, supposedly.

Glass is a fine material. You know how incandescent lamp bulbs are made in various controlled shapes...so,
is there a source of glass vials 6-8mm diameter that could be capped with plastic snap on lids?

Hmmm.... yes, here are 200/$36, or about 0.22 USD each:
http://www.sigmaaldrich.com/catalog/product/aldrich/z291994?lang=en&region=US 8 × 40mm, 1mL vol. Constructed of clear
borosilicate glass. Vials come with LDPE snap-plug caps.

These would be good to design a system around. I'd prefer a little shorter than 4cm though.

Here are shorter ones. http://www.biotechsolutions.org/vials.htm

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
For more options, visit https://groups.google.com/groups/opt_out.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

[DIYbio] Fwd: Open source bioplastic 2012 update

From: eric poliner <polinere@gmail.com>
Date: Mon, Dec 31, 2012 at 12:18 PM
Subject: Open source bioplastic 2012 update
To: Marcin Jakubowski <joseph.dolittle@gmail.com>,
marcin@opensourceecology.org, Joshua Pearce <pearce@mtu.edu>, Jerry
Anzalone <gcanzalo@mtu.edu>, Catarina Mota <catarinamfmota@gmail.com>,
Kerem Bora <kerembora@sabanciuniv.edu>, Jordan Miller
<jmil@hive76.org>, kanzure@gmail.com, cfharr@gmail.com


Hello,

I wanted to provide a year end update on the status of the OSE open
source bioplastic production project to all interested parties.

Overview:
Over past year the open source bioplastic production project has been
focused on collecting background information, proposing preliminary
approaches, and developing collaborations. Polylactic acid,
polyethylene, cellulose acetate, and moldable mycelium are four
different and complementary bioplastics under research and
development. A biorefinery approach to create multiple bioplastics
from a single feedstock has been proposed and appears feasible and in
line with the OSE guidelines. Collaborations with Dr Bora of Sabanci
University and Dr Pearce of Michigan Technological University are
strengthening the project and may allow material progress in the
coming year. A collaboration to produce polylactic acid with Michigan
Tech's Open Sustainability Technology Research Group led by Dr Pearce
will hopefully be the first project to be implemented.

Specific Objectives for 2013:
The primary objective for 2013 is to demonstrate polylactic acid
production over the summer in the laboratory of Dr Pearce. Polylactic
acid is a plastic of moderate complexity, compatible with 3D printing
and other fabrication techniques, and could demonstrate a significant
OS advancement. A production process that produces lactic acid by
microbial fermentation from agricultural waste, refinement to pure
lactic acid with cell and feedstock recycle by membrane separation,
and polymerization by catalytic dehydration is believed to be the most
feasible and efficient route for sustainable localized PLA production.
The proposed approach is believed to be able to eliminate the major
energy consuming step of feedstock sterilization while avoiding
contamination, by using a bacteria (Bacillus coagulans) that grows at
a temperature higher than most other bacteria. Purification of lactic
acid from the fermentation broth through size and charge selective
membranes which will allow continuous fermentation and purification of
pure lactic acid without salt waste. Polymerization via a condensation
reaction which will be conducted under a vacuum with an efficient
catalyst of tin chloride and p-toluenesulfonic acid. Developing the
necessary skills and hardware will take interdisciplinary knowledge
and collaborations but there appears to be the necessary willing
participants. The OSE wiki and appropedia wiki will be used to
coordinate the project demonstrating a new approach to distributed
applied science and open source development of highly technical
production methods. A PLA project worklog has been started to to track
and facilitate project progress.

Broader Impacts:
Creation of OS polylactic acid will demonstrate the productive power
of the open source approach to material progress and incorporate
"green chemistry" into the open source toolkit. Through polylactic
acid it is hoped that open source high-tech modular research/small
scale industrial hardware can be built and its use demonstrated in a
research and enterprise setting. With open source knowledge and
guidance of fermentor, purification, and chemical reactor technology a
new frontier of chemical engineering will be accessible to
experimenters and entrepreneurs. Furthermore, project participants are
interested in developing open source enterprise plans to sell and
profitably develop open source hardware, as well as an OS biorefinery
approaches to production of biofuels and biomaterials.

Future Directions:
Development of open source polylactic acid production will be followed
by refinement of other polymers. Other polymers will require a more
robust chemical reactor but a modular approach to hardware design
should facilitate flexible hardware that can be configured for any
variety of chemical reactions. With multiple polymers available in the
open source sphere copolymers (polylactic acid cellulose acetate,
polethylene vinyl acetate, cellulose acetate polyvinyl difluoride,
etc) can be created for a wide variety applications. This could
represent a significant economic impact that would allow
transformation of low value biomass to high value and high utility
materials and products in a localized market.

Thank you for your support over the last year, and I look forward to
continued progress next year,

Eric


--
- Bryan
http://heybryan.org/
1 512 203 0507

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
For more options, visit https://groups.google.com/groups/opt_out.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

Re: [DIYbio] Which Antibiotics can be autoclaved

I don't know if they will degrade but is there a special reason why they need to go through the autoclave?  Just add them to the media and swirl around before you pour the plates, or whatever it is you are pouring.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
For more options, visit https://groups.google.com/groups/opt_out.
 
 

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

Re: [DIYbio] First full DNA extraction, PCR, and gel

Yup heat transfer is the key, but you can avoid mineral oil with glass capillaries since you flame seal them into a closed environment.  Getting the samples out of the capillaries requires some weird aspirator needle thing though, supposedly.  



It does go to show though, that with the glass capillaries you can do a full 30-35 cycle runs in 10-30 minutes, but they were only amplifying a 250bp target.  


That's the chart that shows plastic vs glass, and below you can see how fast the program times are, and as you say, much higher lag with plastic in red.  Pretty nifty stuff.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
For more options, visit https://groups.google.com/groups/opt_out.
 
 

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

Re: [DIYbio] 0.2 um filters for sterile filtering/possibly viscous solutions

They also make filters to go on bottles:

http://fishersci.com/ecomm/servlet/fsproductdetail_10652_12704331_29104_-1_0?LBCID=26030796

--A

On Mon, Dec 31, 2012 at 12:59 PM, Eugen Leitl <eugen@leitl.org> wrote:
On Mon, Dec 31, 2012 at 12:49:17PM -0500, Avery louie wrote:
> It might be possible to get pre sterilized filtration bottles.  Nalgene
> makes them pretty cheap.  No muss, no fuss, and they are vacuum assisted!

We'll just flush
http://invitroshop.de/shop/laborbedarf/laborglas/laborflasche-simax-371.html
with ultrafiltered reverse osmosis water, and autoclave these prior
of sterile-filtering into them, then autoclave them prior to
fastening the cap.

This is labor-intensive, but the result should have some pretty
good shelf half life.


--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
For more options, visit https://groups.google.com/groups/opt_out.



--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
For more options, visit https://groups.google.com/groups/opt_out.
 
 

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

Re: [DIYbio] 0.2 um filters for sterile filtering/possibly viscous solutions

On Mon, Dec 31, 2012 at 12:49:17PM -0500, Avery louie wrote:
> It might be possible to get pre sterilized filtration bottles. Nalgene
> makes them pretty cheap. No muss, no fuss, and they are vacuum assisted!

We'll just flush
http://invitroshop.de/shop/laborbedarf/laborglas/laborflasche-simax-371.html
with ultrafiltered reverse osmosis water, and autoclave these prior
of sterile-filtering into them, then autoclave them prior to
fastening the cap.

This is labor-intensive, but the result should have some pretty
good shelf half life.


--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
For more options, visit https://groups.google.com/groups/opt_out.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

Re: [DIYbio] 0.2 um filters for sterile filtering/possibly viscous solutions

It might be possible to get pre sterilized filtration bottles.  Nalgene makes them pretty cheap.  No muss, no fuss, and they are vacuum assisted!

--A

On Dec 31, 2012 9:12 AM, "Eugen Leitl" <eugen@leitl.org> wrote:
On Mon, Dec 31, 2012 at 11:08:14AM -0600, John Griessen wrote:

> and flushing out the assembly
>> for an hour with reverse osmosis water, possibly prefiltered.
>
> How would you be sure that RO water stream is sterile?  Run through tubing
> past a UV light source?

The reverse osmosis plant arguably does UV-sterilization but there's
no accounting for biofilm colonization downstream. I will check using
a microscope, of course, but for time being I consider the reverse
osmosis water to be bacterially contaminated. I will make sure that
everything downstream of the filter will be sterile, and the filter
assembly itself will be sterilized by cold wet sterilization (abovementioned
soaking) prior to flushing it.

In case of success all the biocontaminants will be contained within
the ultrafilter. Residual ones will be killed during autoclaving.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
For more options, visit https://groups.google.com/groups/opt_out.


--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
For more options, visit https://groups.google.com/groups/opt_out.
 
 

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

Re: [DIYbio] 0.2 um filters for sterile filtering/possibly viscous solutions

On Mon, Dec 31, 2012 at 11:08:14AM -0600, John Griessen wrote:

> and flushing out the assembly
>> for an hour with reverse osmosis water, possibly prefiltered.
>
> How would you be sure that RO water stream is sterile? Run through tubing
> past a UV light source?

The reverse osmosis plant arguably does UV-sterilization but there's
no accounting for biofilm colonization downstream. I will check using
a microscope, of course, but for time being I consider the reverse
osmosis water to be bacterially contaminated. I will make sure that
everything downstream of the filter will be sterile, and the filter
assembly itself will be sterilized by cold wet sterilization (abovementioned
soaking) prior to flushing it.

In case of success all the biocontaminants will be contained within
the ultrafilter. Residual ones will be killed during autoclaving.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
For more options, visit https://groups.google.com/groups/opt_out.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

Re: [DIYbio] 0.2 um filters for sterile filtering/possibly viscous solutions

On 12/31/2012 10:43 AM, Eugen Leitl wrote:
> At this point I'm thinking about overnight soaking the ceramics
> filter/whole filtering assembly in hydrogen carbonate/hydrogen
> peroxide, 70% isopropanol or bleach,

Sounds good. No experience though.

and flushing out the assembly
> for an hour with reverse osmosis water, possibly prefiltered.

How would you be sure that RO water stream is sterile? Run through tubing
past a UV light source?

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
For more options, visit https://groups.google.com/groups/opt_out.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

Re: [DIYbio] 0.2 um filters for sterile filtering/possibly viscous solutions

On Mon, Dec 31, 2012 at 11:53:27AM -0500, Sebastian S. Cocioba wrote:
> Almost all PP is autoclavable but may risk slight deformation over a long period of time. At standard autoclave temp of 121 C it will be 9c below polyprop melting point. I sterilize my eppitubes, cutting sheets, etc if i see the PP 5 symbol on the plastic. Tips may deform if your autoclave is not steady at 121.

Thanks, but this is a 0.2 um PP filter. Not only will it warp to the point where
it might leak at the sames, it will likely ruin the pore size.

I'm not sure how gentle the local autoclaves are, as this is a shared
facility. It will certainly reach 121 deg C, but is arguably likely to
exceed it.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
For more options, visit https://groups.google.com/groups/opt_out.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

Re: [DIYbio] 0.2 um filters for sterile filtering/possibly viscous solutions

Almost all PP is autoclavable but may risk slight deformation over a long period of time. At standard autoclave temp of 121 C it will be 9c below polyprop melting point. I sterilize my eppitubes, cutting sheets, etc if i see the PP 5 symbol on the plastic. Tips may deform if your autoclave is not steady at 121.

Sebastian S Cocioba
CEO & Founder
New York Botanics, LLC

Sent via Mobile E-Mail

On Dec 31, 2012, at 11:43 AM, Eugen Leitl <eugen@leitl.org> wrote:

>
> Happy $holidays!
>
> A quick question: I need to sterile-filter several 10 l of a
> reasonably viscous solution into autoclavable borosilicate
> lab bottles.
>
> This is typically done with syringe filters,
>
> http://www.gilsonuk.com/catalogue/product/itemNo/ALG422A
>
> but that's on a too small scale, and for rather nonviscous
> solutions.
>
> I was thinking about repurposing household items like
> http://www.amazon.de/Keramikfilter-Wasserfilter-NEU-Filter-Feinfilter/dp/B002RLKE7O/
> or http://www.amazon.de/Polypropylene-Auswaschbar-UMKEHROSMOSE-WASSERFILTER-SEDIMENTFILTER/dp/B002RLZS7U/
>
> Has any of you done that? How did you sterilize the assembly?
> (Polypropylene is obviously only cold-sterilizable, and
> I suspect the plastic parts of the ceramics one won't
> survive autoclaving either).
>
> At this point I'm thinking about overnight soaking the ceramics
> filter/whole filtering assembly in hydrogen carbonate/hydrogen
> peroxide, 70% isopropanol or bleach, and flushing out the assembly
> for an hour with reverse osmosis water, possibly prefiltered.
>
> Thanks and a nice 2013 to ye all.
>
> --
> -- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
> Learn more at www.diybio.org
> ---
> You received this message because you are subscribed to the Google Groups "DIYbio" group.
> To post to this group, send email to diybio@googlegroups.com.
> To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
> Visit this group at http://groups.google.com/group/diybio?hl=en.
> For more options, visit https://groups.google.com/groups/opt_out.
>
>

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
For more options, visit https://groups.google.com/groups/opt_out.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

[DIYbio] 0.2 um filters for sterile filtering/possibly viscous solutions

Happy $holidays!

A quick question: I need to sterile-filter several 10 l of a
reasonably viscous solution into autoclavable borosilicate
lab bottles.

This is typically done with syringe filters,

http://www.gilsonuk.com/catalogue/product/itemNo/ALG422A

but that's on a too small scale, and for rather nonviscous
solutions.

I was thinking about repurposing household items like
http://www.amazon.de/Keramikfilter-Wasserfilter-NEU-Filter-Feinfilter/dp/B002RLKE7O/
or http://www.amazon.de/Polypropylene-Auswaschbar-UMKEHROSMOSE-WASSERFILTER-SEDIMENTFILTER/dp/B002RLZS7U/

Has any of you done that? How did you sterilize the assembly?
(Polypropylene is obviously only cold-sterilizable, and
I suspect the plastic parts of the ceramics one won't
survive autoclaving either).

At this point I'm thinking about overnight soaking the ceramics
filter/whole filtering assembly in hydrogen carbonate/hydrogen
peroxide, 70% isopropanol or bleach, and flushing out the assembly
for an hour with reverse osmosis water, possibly prefiltered.

Thanks and a nice 2013 to ye all.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
For more options, visit https://groups.google.com/groups/opt_out.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

Re: [DIYbio] First full DNA extraction, PCR, and gel

Sounds like the main bottleneck with thethe Rapidcycler is the heat transfer from and to the sample - hence the use of glass capillaries and mineral oil. If you're using plastic PCR tubes, you should count on a much longer ramp time. That's probably why the Peltier was more efficient.

Patrik

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/XkeeEEXQ_WUJ.
For more options, visit https://groups.google.com/groups/opt_out.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

[DIYbio] Smallest aliquot for dinos?

Two issues to worry about would be the low cell counts, and any tendency to stick to the side walls. I'd say give it a try, but you might encounter more variability than is acceptable to you.

Patrik

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/aRNXLTSoaJoJ.
For more options, visit https://groups.google.com/groups/opt_out.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

[DIYbio] Smallest aliquot for dinos?

Anyone ever try to grow dinos in small ~1ml aliquots?  It seems like they would be ok, provided they didnt dry up.  Just curious.

--A

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
For more options, visit https://groups.google.com/groups/opt_out.
 
 

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

[DIYbio] Which Antibiotics can be autoclaved

Hi everybody, 


for our GFP - plant project at the AEC we will need Kanamycin, Tetracyclin and Streptomycin. 


AFAIK, you can autoclave Kanamycin (but never ever ever Ampicillin! ;) ). What about Tetracyclin and Streptomycin, will they degrade @ 121°C and 2 bars? 


Best, 
Andreas

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/KpCEmx2c9s4J.
For more options, visit https://groups.google.com/groups/opt_out.
 
 

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

[Tue 25 Dec to Mon 31 Dec] - This week Jobs on all Newspapers

Tue 25 Dec to Mon 31 Dec - Pakistan Newspaper Jobs on Jang, Nawa e Waqt, Express, Dawn, The Nation, The News Newspapers for this week.

Note: If you use Digest Email Option then HTML formatting will not work well, browse jobs manually from our webiste directly: http://www.paperparho.com/

Lecturers, Accountants, Libraians and Receptionist Jobs in Suffa Institute of Medical Sciences Peshawar
Published on Daily Aaj at 31 December 2012

Librarians, Junior Clerks and Electricians Jobs in Government Organization Khyber Pakhtunkhwa
Published on Daily Aaj at 31 December 2012

Builders and Developers Jobs in Lahore
Published on The News at 31 December 2012

Different Jobs 1 in In Different Cities Nationwide
Published on Daily Khabrain at 31 December 2012

Security Staff Jobs in Lahore
Published on Daily Nawa e Waqt at 31 December 2012

Different Jobs 1 in Karachi and Adjacent Cities
Published on Daily Express at 31 December 2012

Sales Officers, Sales Supervisor and Sales Man Jobs in Diamond Trading Company (DTC) Lahore
Published on Daily Express at 31 December 2012

Builders and Developers Jobs in Lahore
Published on Daily Express at 31 December 2012

Sales Officers Jobs in Faisalabad
Published on Daily Express at 31 December 2012

Technical and Other Jobs 3 in Saudia Arabia
Published on Daily Express at 31 December 2012

Technical and Other Jobs 6 in UAE
Published on Daily Express at 31 December 2012

Technical and Other Jobs 2 in Abu Dhabi
Published on Daily Express at 31 December 2012

Technical and Other Jobs 1 in Malaysia
Published on Daily Express at 31 December 2012

Technical and Other Jobs 2 in Saudia Arabia
Published on Daily Express at 31 December 2012

Technical and Other Jobs 5 in UAE
Published on Daily Express at 31 December 2012

Technical and Other Jobs 2 in Oman
Published on Daily Express at 31 December 2012

Technical and Other Jobs 4 in UAE
Published on Daily Express at 31 December 2012

Technical and Other Jobs 3 in UAE
Published on Daily Express at 31 December 2012

Technical and Other Jobs 1 in Abu Dhabi
Published on Daily Express at 31 December 2012

Technical and Other Jobs 1 in Oman
Published on Daily Express at 31 December 2012

Technical and Other Jobs 2 in UAE
Published on Daily Express at 31 December 2012

Technical and Other Jobs 1 in UAE
Published on Daily Express at 31 December 2012

Technical and Other Jobs 1 in Saudia Arabia
Published on Daily Express at 31 December 2012

Different Jobs 1 in Karachi and Adjacent Cities
Published on Daily Jang at 31 December 2012

Different Jobs 2 in Lahore and Adjacent Cities
Published on Daily Jang at 31 December 2012

Different Jobs 1 in Lahore and Adjacent Cities
Published on Daily Jang at 31 December 2012

Instructors Leather Garments and Instructors Leather Good and Gloves Jobs in TEVTA Institute of Leather Technology Gujranwala
Published on Daily Jang at 31 December 2012

Different Jobs 3 in Islamabad and Adjacent Cities
Published on Daily Jang at 31 December 2012

Different Jobs 2 in Islamabad and Adjacent Cities
Published on Daily Jang at 31 December 2012

Different Jobs 1 in Islamabad and Adjacent Cities
Published on Daily Jang at 31 December 2012

Medical Officers Jobs in Gyne Hospital Islamabad
Published on Daily Jang at 31 December 2012

Technical and Other Jobs 3 in Abu Dhabi
Published on Daily Aaj at 30 December 2012

Data Analyst and Investigators Jobs in Khyber Pakhtunkhwa
Published on Daily Aaj at 30 December 2012

FCPS II Trainee and Senior Registrars Jobs in Fatima Memorial Hospital Lahore
Published on Daily Dawn at 30 December 2012

Lineman Training Coordinators and Facilitators Jobs in Nation Wide
Published on Daily Dawn at 30 December 2012

Finanace, Marketing and Junior Investment Advisors Jobs in Board of Investment (BOI) Islamabad
Published on Daily Dawn at 30 December 2012

Engineers and General Managers Jobs in National Transmission and Despatch Comapny Limited Lahore
Published on Daily Dawn at 30 December 2012

Manager Admin, Manager Security and Assistant Chef Jobs in Karachi
Published on Daily Dawn at 30 December 2012

Pakistan Army Captain Jobs in Multan
Published on The News at 30 December 2012

Consultants, Managers, Research Officers and Other Jobs in Awaz Foundation Pakistan
Published on The News at 30 December 2012

Finanace Officers, Aducators Officers, Coordinators and Other Jobs in United Nations Childrens Fund (UNICEF)
Published on The News at 30 December 2012

Directors, IT Managers, HR Administration, Officers, Scientists, Finanace, Accountants and Lot of Other Jobs in Pakistan Central Cotton Committee Karachi
Published on The News at 30 December 2012

Program Officers Jobs in Micronutrient Initiative Islamabad
Published on The News at 30 December 2012

Office Assistant Jobs in Ministry of Information Technology
Published on The Nation at 30 December 2012

Pakistan Army Captain Jobs in Multan
Published on The Nation at 30 December 2012

Data Analyst and Investigators Jobs in Khyber Pakhtunkhwa
Published on Daily Mashriq at 30 December 2012

Caretakers Jobs in Khyber Pakhtunkhwa
Published on Daily Mashriq at 30 December 2012

Stenotypists and Other Jobs in District and Session Judge Hangu
Published on Daily Mashriq at 30 December 2012

ICU, NICU, CCU and ER Nurses Staff Jobs in Shifa International Hospital Limited
Published on Daily Mashriq at 30 December 2012

Union Council Polio Workers (UCPWs) Jobs in World Health Organization (WHO) Khyber Pakhtunkhwa
Published on Daily Mashriq at 30 December 2012

Finance Officers, Education Officers, Coordinators and Other Jobs in United Nations Childrens Fund (UNICEF)
Published on Daily Mashriq at 30 December 2012

Technical and Other Jobs 1 in UAE
Published on Daily Mashriq at 30 December 2012

GIS Surveyors and GIS Technicians Jobs in PITCO Lahore
Published on Daily Mashriq at 30 December 2012

Technical and Other Jobs 1 in Malaysia
Published on Daily Mashriq at 30 December 2012

Computer Operators, Accountants, Security Guards and Other Jobs in Adil International (Pvt) Limited Nowshera
Published on Daily Mashriq at 30 December 2012

Different Jobs 1 in In Different Cities Nationwide
Published on Daily Khabrain at 30 December 2012

Drivers, Naib Qasid and Other Jobs in Lahore Development Authority
Published on Daily Nawa e Waqt at 30 December 2012

Nurses, Accountants, UDC, LDC, Stenotypists, Teachers, Security Staff and Lot of Other Jobs in Militry College Sui
Published on Daily Nawa e Waqt at 30 December 2012

Computer Operators, Assistant and Other Jobs in CMH Medical Lahore College
Published on Daily Nawa e Waqt at 30 December 2012

Assistant Professors, Lecturers, Admin Officers, Accountants, Security Staff and Other Jobs in Muhammad Nawaz Sharif University Multan
Published on Daily Nawa e Waqt at 30 December 2012

Different Jobs 2 in Karachi and Adjacent Cities
Published on Daily Express at 30 December 2012

Different Jobs 1 in Karachi and Adjacent Cities
Published on Daily Express at 30 December 2012

Junior Programmer and Office Supervisors Jobs in Karachi Shipyard and Engineering Works Limited
Published on Daily Express at 30 December 2012

Office Assistant Jobs in Ministry of Information Technology
Published on Daily Express at 30 December 2012

Van Helpers and Loaders Jobs in Karachi
Published on Daily Express at 30 December 2012

Principal, Teachers, ICT Officers and Coordintors Jobs in City Campus Thatta
Published on Daily Express at 30 December 2012

Technical and Other Jobs 6 in UAE
Published on Daily Express at 30 December 2012

Engineers and General Managers Jobs in National Transmission and Despatch Comapny Limited Lahore
Published on Daily Express at 30 December 2012

Technical and Other Jobs 5 in Saudia Arabia
Published on Daily Express at 30 December 2012

Technical and Other Jobs 4 in Saudia Arabia
Published on Daily Express at 30 December 2012

Technical and Other Jobs 5 in UAE
Published on Daily Express at 30 December 2012

Technical and Other Jobs 3 in Saudia Arabia
Published on Daily Express at 30 December 2012

Technical and Other Jobs 1 in Malaysia
Published on Daily Express at 30 December 2012

Sales Officer and Sales Man Jobs in Lahore
Published on Daily Express at 30 December 2012

Technical and Other Jobs 2 in Saudia Arabia
Published on Daily Express at 30 December 2012

Sales Managers, Area Managers and Other Jobs in Lahoe
Published on Daily Express at 30 December 2012

Different Jobs 1 in In Different Cities Nationwide
Published on Daily Express at 30 December 2012

Different Jobs 1 in Islamabad and Adjacent Cities
Published on Daily Express at 30 December 2012

Technical and Other Jobs 4 in UAE
Published on Daily Express at 30 December 2012

Technical and Other Jobs 3 in UAE
Published on Daily Express at 30 December 2012

Technical and Other Jobs 1 in Oman
Published on Daily Express at 30 December 2012

Male and Female Information Officers Jobs in Rawalpindi
Published on Daily Express at 30 December 2012

Technical and Other Jobs 1 in Libya
Published on Daily Express at 30 December 2012

Technical and Other Jobs 2 in UAE
Published on Daily Express at 30 December 2012

Technical and Other Jobs 1 in UAE
Published on Daily Express at 30 December 2012

Technical and Other Jobs 1 in Saudia Arabia
Published on Daily Express at 30 December 2012

Mechanical Transport Incharge Jobs in Lahore
Published on Daily Express at 30 December 2012

Office Assistant Jobs in Ministry of Information Technology
Published on Daily Express at 30 December 2012

Technical and Other Jobs 13 in Saudia Arabia
Published on Daily Jang at 30 December 2012

Engineers, Foreman and Other Jobs in Saudi Arabia
Published on Daily Jang at 30 December 2012

Different Jobs in Malir Cantt
Published on Daily Jang at 30 December 2012

Different Jobs 18 in Karachi and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 17 in Karachi and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 16 in Karachi and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 15 in Karachi and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 14 in Karachi and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 13 in Karachi and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 12 in Karachi and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 17 in Lahore and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 11 in Karachi and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 10 in Karachi and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 9 in Karachi and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 8 in Karachi and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 7 in Karachi and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 6 in Karachi and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 5 in Karachi and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 4 in Karachi and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 3 in Karachi and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 2 in Karachi and Adjacent Cities
Published on Daily Jang at 30 December 2012

Junior Programmer and Office Supervisors Jobs in Karachi Shipyard and Engineering Works Limited
Published on Daily Jang at 30 December 2012

Technical and Other Jobs 12 in Saudia Arabia
Published on Daily Jang at 30 December 2012

Managers, Accountants and Other Jobs in Multinational Company Karachi
Published on Daily Jang at 30 December 2012

Legal Advisors Jobs in Karachi Shipyard and Engineering Works Limited
Published on Daily Jang at 30 December 2012

Accountants Jobs in Karachi
Published on Daily Jang at 30 December 2012

Radiology, Obs Gynecologigy, ENT, Urology, Nurses and Lot of Other Healthcare Jobs in Saudi Arabia
Published on Daily Jang at 30 December 2012

Principal and Female Teachers (All Subjects) Jobs in TCF Schools Karachi
Published on Daily Jang at 30 December 2012

Principal, Teachers, ICT Officers and Coordintors Jobs in City Campus Thatta
Published on Daily Jang at 30 December 2012

Speech Therapists Jobs in PAF Special Children School Karachi
Published on Daily Jang at 30 December 2012

Different Jobs 1 in Karachi and Adjacent Cities
Published on Daily Jang at 30 December 2012

Nurses, Physics, Chemistry, Maths, Biology, Computer Teachers and Other Different Subjects Teachers Jobs in Fazaia Inter College Karachi
Published on Daily Jang at 30 December 2012

Technical and Other Jobs 3 in UAE
Published on Daily Jang at 30 December 2012

Managers, Engineers, Designers, Marketing, Sales Staff and Lot of Other Jobs in M Tech Multi Technology (Pvt) Limited Lahore
Published on Daily Jang at 30 December 2012

Cheif Accountants, Accountants and Sales Executives Jobs in Karachi
Published on Daily Jang at 30 December 2012

Technical and Other Jobs 11 in Saudia Arabia
Published on Daily Jang at 30 December 2012

Stenotypists, Lower Division Clerk (LDC), Drivers and Other Jobs in Karachi Logistics Area
Published on Daily Jang at 30 December 2012

Doctors, Security Staff and Other Jobs in LRBT Karachi
Published on Daily Jang at 30 December 2012

Computer Operators, Computer Technicians, Data Entry Operators, Technicians, Security Staff and Other Jobs in Karachi
Published on Daily Jang at 30 December 2012

Technical and Other Jobs 10 in Saudia Arabia
Published on Daily Jang at 30 December 2012

Different Jobs 16 in Lahore and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 15 in Lahore and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 14 in Lahore and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 13 in Lahore and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 12 in Lahore and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 11 in Lahore and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 10 in Lahore and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 9 in Lahore and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 8 in Lahore and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 7 in Lahore and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 6 in Lahore and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 5 in Lahore and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 4 in Lahore and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 3 in Lahore and Adjacent Cities
Published on Daily Jang at 30 December 2012

Website Developers, Website Designers and Tele Marketing Jobs in School Of Advance Business and Commerce (SABAC) Faisalabad
Published on Daily Jang at 30 December 2012

Nurses, Admin Officers, Receptionists and Other Jobs in Lahore
Published on Daily Jang at 30 December 2012

Technical and Other Jobs 2 in Abu Dhabi
Published on Daily Jang at 30 December 2012

Directors Sales Agents Jobs in UAE
Published on Daily Jang at 30 December 2012

HVAC Engineers, Supervisors, Technicians and Other Jobs in Green Technologies Lahore
Published on Daily Jang at 30 December 2012

Administration Officers, Security Staff, Sales Staff and Labours Jobs in Lahore
Published on Daily Jang at 30 December 2012

Telephone Operators and Receptionists Jobs in Chanar Sugar Mills Limited Lahore
Published on Daily Jang at 30 December 2012

Tele Sales Representatives Jobs in Icon Marketing Faisalabad
Published on Daily Jang at 30 December 2012

Field Sales Staff Jobs in Smart Shops Pakistan Limited Lahore
Published on Daily Jang at 30 December 2012

Principal, Vice Principal, Teacgers (All Subjects), Admin Officers and Other Jobs in Campus Plus Coaching Institute Lahore
Published on Daily Jang at 30 December 2012

Technical and Other Jobs 9 in Saudia Arabia
Published on Daily Jang at 30 December 2012

Engineers and Shuttering Foreman Jobs in Construction Company Lahore
Published on Daily Jang at 30 December 2012

Programmer Analyst and IT Staff Jobs in Smart Shops Pakistan Limited Lahore
Published on Daily Jang at 30 December 2012

Sales Oficers Jobs in Lahore
Published on Daily Jang at 30 December 2012

Town Planners, GIS Experts and GIS Digitizers Jobs in Master Consulting Engineers (Pvt) Limited Lahore
Published on Daily Jang at 30 December 2012

Accountants, Admin, Sales / Sevices Executives and Other Jobs in Medical Trade Organization Lahore
Published on Daily Jang at 30 December 2012

Medical Specialists, Physicians, Cardiologists, Orthopedic Surgeon, ENT Surgeon, Medical Officers and Lot of Other Jobs in Hijaz Teaching Hospital Lahore
Published on Daily Jang at 30 December 2012

Junior Investment Advisors Jobs in Board of Investment (BOI) Islamabad
Published on Daily Jang at 30 December 2012

Subject Specialists (All Subjects), Finanace Managers, Customers Relation Officers and Front Desk Officers Jobs in The Future Schools (Pvt) Limited Lahore
Published on Daily Jang at 30 December 2012

Security Officers Jobs in Multan
Published on Daily Jang at 30 December 2012

Computer Operators, Assistants and Other Jobs in CMH Medical Lahore College
Published on Daily Jang at 30 December 2012

Cosmetic Specialists, Pet Food Specialists, Supervisors, Cashiers and Other Jobs in Bahria Town (Pvt) Limited Lahore
Published on Daily Jang at 30 December 2012

MBBS Doctors, Managers, Executives and Female Laser Procedure Trainees Jobs in Laser Skin Clinic Lahore
Published on Daily Jang at 30 December 2012

Professors, Associate Professors and Assistant Professors Jobs in GC University Faisalabad
Published on Daily Jang at 30 December 2012

Senior Specialists, Specialists and Management Jobs in The Urban Unit Lahore
Published on Daily Jang at 30 December 2012

Sr Software Engineers, Graphic Designers, Architects and Surveyors Jobs in Bahria Town (Pvt) Limited Rawalpindi
Published on Daily Jang at 30 December 2012

Lecturers, Web Designers, Web Developers, Managers and Administrators Jobs in Tourism Development Corporation of Punjab Lahore
Published on Daily Jang at 30 December 2012

Graphic Designers and Sales Officers Jobs in Lahore and Peshawar
Published on Daily Jang at 30 December 2012

Professors Jobs in GC University Faisalabad
Published on Daily Jang at 30 December 2012

Different Jobs 2 in Lahore and Adjacent Cities
Published on Daily Jang at 30 December 2012

Medical Officers, Sales Staff, Security Staff, Depty Manager Operation (Imports) and Lot of Other Jobs in Lahore
Published on Daily Jang at 30 December 2012

Different Jobs 1 in Lahore and Adjacent Cities
Published on Daily Jang at 30 December 2012

Principal Jobs in Divisional Public School Pakpattan
Published on Daily Jang at 30 December 2012

Medical Officers, Nurses, Computer Hardware / IT Experts, Accountant and Lot of Other Jobs in Omar Hospital and Cardiac Center Lahore
Published on Daily Jang at 30 December 2012

Branch Managers, Supervisors and Cashiers Jobs in Lahore
Published on Daily Jang at 30 December 2012

Graphic Designers, Sales Officers, Superviors, Security Staff and Other Jobs in Lahore
Published on Daily Jang at 30 December 2012

Technical and Other Jobs 1 in Abu Dhabi
Published on Daily Jang at 30 December 2012

Sales Executives and Textile Designers Jobs in Lahore
Published on Daily Jang at 30 December 2012

Audit Managers and HR Managers Jobs in Afzal Group Of Electronics Lahore
Published on Daily Jang at 30 December 2012

Stenotypists, Lower Division Clerk (LDC), Drivers and Other Jobs in Karachi Logistics Area
Published on Daily Jang at 30 December 2012

Technical and Other Jobs 8 in Saudia Arabia
Published on Daily Jang at 30 December 2012

Managers, Hostess, Trainee Officers and Other Jobs in Freddys Cafe Lahore
Published on Daily Jang at 30 December 2012

Technical Sales Officers and Trainee Sales Offiers Jobs in Swat Agro Chemical Lahore
Published on Daily Jang at 30 December 2012

Marketing Executives, Sales Executives, Technical Staff and Secretary Jobs in Lahore
Published on Daily Jang at 30 December 2012

Project Managers ERP and IT Jobs in Public Utility Company Islamabad
Published on Daily Jang at 30 December 2012

Bio Medical Enineers Jobs in Fauji Foundation Hospital Lahore
Published on Daily Jang at 30 December 2012

Managers, Engineers, Office Assistants, Receptionist and Other Jobs
Published on Daily Jang at 30 December 2012

Call Center Agents Jobs in Lahore
Published on Daily Jang at 30 December 2012

Technical and Other Jobs 7 in Saudia Arabia
Published on Daily Jang at 30 December 2012

Consultant Anaesthetist and FCPS II Trainee Jobs in Ittefaq Hospital Trust Lahore
Published on Daily Jang at 30 December 2012

Subject Specialists (All Subjects) Jobs in Lahore
Published on Daily Jang at 30 December 2012

National Sales Managers Jobs in Tarobina Corporation Lahore
Published on Daily Jang at 30 December 2012

Sales Managers, Accountants, Sales Supervisors, Sales Officers and Store Incharge Jobs in Lahore
Published on Daily Jang at 30 December 2012

Supervisors, Assistant Operators and Trainee Operators Jobs in Corrobox Industries Lahore
Published on Daily Jang at 30 December 2012

Branch Managers, Cashiers and Field Salesman Jobs in Lahore
Published on Daily Jang at 30 December 2012

Medical Specialists and Gynecologists Jobs in Lahore
Published on Daily Jang at 30 December 2012

Marketing Officers, Client Counsellors, Front Desk Officers and Receptionists Jobs in Restore International Hair Transplant Surgery Center Lahore
Published on Daily Jang at 30 December 2012

Security Supervisors, Security Assistant Supervisors and Other Jobs in Paragon City (Pvt) Limited Lahore
Published on Daily Jang at 30 December 2012

Office Staff, Sales Executives, Sub Distributers and Other Jobs in Lahore
Published on Daily Jang at 30 December 2012

Dental Surgeons, Dental Technicians and Dental Assistants Jobs in Hamza Foundation Lahore
Published on Daily Jang at 30 December 2012

Technical and Other Jobs 6 in Saudia Arabia
Published on Daily Jang at 30 December 2012

Sales Executives, Accounts Officers, Managers, Security Staff and Other Jobs in Nizami Brothers Lahore
Published on Daily Jang at 30 December 2012

Sales Managers, Engineers, Credit Controllers, Finance Staff, Supervisors and Other Jobs in Ghani Gases Limited Lahore
Published on Daily Jang at 30 December 2012

Development Managers, Farm Managers and Other Jobs in Muzaffargarh
Published on Daily Jang at 30 December 2012

Managers, HR Officers, Microbiologists and Chemists Jobs in Medipak Limited Lahore
Published on Daily Jang at 30 December 2012

Marketing and Sales Jobs in Nation Wide
Published on Daily Jang at 30 December 2012

Technical and Other Jobs 2 in UAE
Published on Daily Jang at 30 December 2012

Engineers, Managers, Technicians and Lot of Other Jobs in Saudi Arabia
Published on Daily Jang at 30 December 2012

Managers, Engineers, Designers, Marketing, Sales and Lot of Other Jobs in M Tech Multi Technology (Pvt) Limited Lahore
Published on Daily Jang at 30 December 2012

Sub Inspectors and ASI Jobs in Federal Investigation Agency (FIA) Islamabad
Published on Daily Jang at 30 December 2012

Engineers, Inspectors, Supervisors and Lot of Other Jobs in Saudi Arabia
Published on Daily Jang at 30 December 2012

Engineers, Mechanics, Electricians, Sales Managers and Lot of Other Jobs in Saudi Arabia
Published on Daily Jang at 30 December 2012

Directors, IT Managers, HR Administration, Officers, Scientists, Accountants and Lot of Other Jobs in Pakistan Central Cotton Committee Karachi
Published on Daily Jang at 30 December 2012

Directors, Assistants, Photographers, Lower Division Clerk (LDC) Jobs in International Islamic University Islamabad (IIUI)
Published on Daily Jang at 30 December 2012

Engineers, Technicians and Lot of Other Jobs in Saudi Arabia
Published on Daily Jang at 30 December 2012

Technical and Other Jobs 5 in Saudia Arabia
Published on Daily Jang at 30 December 2012

Security Staff Jobs in Karakoram Security Services Rawalpindi
Published on Daily Jang at 30 December 2012

Technical and Other Jobs 4 in Saudia Arabia
Published on Daily Jang at 30 December 2012

Technical and Other Jobs 3 in Saudia Arabia
Published on Daily Jang at 30 December 2012

Technical and Other Jobs 2 in Saudia Arabia
Published on Daily Jang at 30 December 2012

Managers, Site Engineers, and Protection Engineers Jobs in Saudi Arabia
Published on Daily Jang at 30 December 2012

Technical and Other Jobs 1 in UAE
Published on Daily Jang at 30 December 2012

Technical and Other Jobs 1 in Saudia Arabia
Published on Daily Jang at 30 December 2012

Salesman and Salesman Assistants Jobs in Saudi Arabia
Published on Daily Jang at 30 December 2012

CPO, Officers and Other Jobs in Multinational Company Islamabad
Published on Daily Jang at 30 December 2012

Female Staff Nurses Jobs in Rawalpindi
Published on Daily Jang at 30 December 2012

Call Center Representatives Jobs in Rawalpindi
Published on Daily Jang at 30 December 2012

Stenotypists, Officers, Computer Assistants, Security Staff and Other Jobs in National Saving Department Islamabad
Published on Daily Jang at 30 December 2012

Electronics System Design Engineers Jobs in EST (Pvt) Limited Rawalpindi
Published on Daily Jang at 30 December 2012

Professors, Associate Professors, Assistant Professors, Lecturers and Lot of Other Jobs in Institute of Business Administration (IBA)
Published on Daily Jang at 30 December 2012

HR, Administration, Finance Officers and Managers Jobs in Water and Sanitation Agency (WASA) Peshawar
Published on Daily Jang at 30 December 2012

Site Engineers Jobs in Construction Company Rawalpindi
Published on Daily Jang at 30 December 2012

SM Group Students, Drivers, Pointsman and Lot of Other Jobs in Pakistan Railways Rawalpindi
Published on Daily Jang at 30 December 2012

Urdu, English, Biology, Physics, Maths, Computer Teachers, Lab Assistants and Other Jobs in Askari Cement Ltd Wah, Rawalpindi
Published on Daily Jang at 30 December 2012

CSRs Jobs in UK
Published on Daily Jang at 30 December 2012

Salesman and Supervisors Jobs in Rawalpindi
Published on Daily Jang at 30 December 2012

Stenotypists, Nurses, Teachers, UDC, LDC, Security Staff, Supervisors and Other Jobs in Militry College Sui
Published on Daily Jang at 30 December 2012

Electrical Engineering, Software Engineering, Business Studies, Accounting and Finanace Programmes Faculty Jobs in Army Public College of Management and Siences (APCOMS) Lahore
Published on Daily Jang at 30 December 2012

Project Engineers and Site Engineers Jobs in Construction Company Rawalpindi
Published on Daily Jang at 30 December 2012

Junior Investment Advisors Jobs in Board of Investment (BOI) Islamabad
Published on Daily Jang at 30 December 2012

Design Engineering, Manufacturing Engineering, Solid Mechanics, Computational Mechanics Faculty and Other Faculty Jobs in Air University Islamabad
Published on Daily Jang at 30 December 2012

Production Managers, QC Assistant Managers Jobs in PIC Technologies Pvt Limited Haripur
Published on Daily Jang at 30 December 2012

Stenotypists, UDC, LDC, Operators, Conductors and Lot Of Other Jobs in National College of Arts Rawalpindi
Published on Daily Jang at 30 December 2012

Different Jobs 11 in Islamabad and Adjacent Cities
Published on Daily Jang at 30 December 2012

Trainee Engineers and Trainee Supervisors Jobs in Askari Cement Nizampur Rawalpindi
Published on Daily Jang at 30 December 2012

Assistant Managers and Officers Jobs in Askari Cement Nizampur Rawalpindi
Published on Daily Jang at 30 December 2012

Different Jobs 10 in Islamabad and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 9 in Islamabad and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 8 in Islamabad and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 7 in Islamabad and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 6 in Islamabad and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 5 in Islamabad and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 4 in Islamabad and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 3 in Islamabad and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 2 in Islamabad and Adjacent Cities
Published on Daily Jang at 30 December 2012

Different Jobs 1 in Islamabad and Adjacent Cities
Published on Daily Jang at 30 December 2012

Managers, Computer Operators, Supervisors, Cashiers and Sales Staff Jobs in Mezban Stores Islamabad
Published on Daily Jang at 30 December 2012

Technical and Other Jobs 2 in Abu Dhabi
Published on Daily Aaj at 29 December 2012

Civil, Electrical, Mechanical, Materials and Geology Jobs in Frontier Works Organization (FWO) Rawalpindi (NTS)
Published on Daily Dawn at 29 December 2012

SM Group Students, Drivers, Pointsman and Lot of Other Jobs in Pakistan Railways Rawalpindi
Published on The Nation at 29 December 2012

Specialists, Finanace, Procurement Management, Research Associate, Research Assistant and Other Jobs in The Urban Unit Lahore
Published on The Nation at 29 December 2012

Different Jobs 1 in In Different Cities Nationwide
Published on Daily Khabrain at 29 December 2012

Different Jobs in Punjab Metrobus Authority Lahore
Published on Daily Nawa e Waqt at 29 December 2012

Different Jobs (Interview Schedule) in Lahore
Published on Daily Nawa e Waqt at 29 December 2012

Specialists (Finance and Administration) Jobs in Pakistan Railways Lahore
Published on Daily Nawa e Waqt at 29 December 2012

Medical Staff Jobs in Lahore
Published on Daily Nawa e Waqt at 29 December 2012

Technical and Other Jobs 6 in UAE
Published on Daily Express at 29 December 2012

Different Jobs 1 in Lahore and Adjacent Cities
Published on Daily Express at 29 December 2012

Lecturers, Web Designers, Web Developers, Managers and Administrators Jobs in Tourism Development Corporation of Punjab Lahore
Published on Daily Express at 29 December 2012

Technical and Other Jobs 5 in UAE
Published on Daily Express at 29 December 2012

Technical and Other Jobs 4 in Saudia Arabia
Published on Daily Express at 29 December 2012

Revenue Assistants Jobs in Punjab Industrial Estates Development and Management Company Lahore
Published on Daily Express at 29 December 2012

Different Jobs 1 in Islamabad and Adjacent Cities
Published on Daily Express at 29 December 2012

Technical and Other Jobs 3 in Saudia Arabia
Published on Daily Express at 29 December 2012

Revenue Assistants Jobs in Punjab Industrial Estates Development and Management Company Lahore
Published on Daily Express at 29 December 2012

Technical and Other Jobs 4 in UAE
Published on Daily Express at 29 December 2012

Technical and Other Jobs 2 in Saudia Arabia
Published on Daily Express at 29 December 2012

Technical and Other Jobs 3 in Abu Dhabi
Published on Daily Express at 29 December 2012

Technical and Other Jobs 1 in Saudia Arabia
Published on Daily Express at 29 December 2012

Technical and Other Jobs 2 in Abu Dhabi
Published on Daily Express at 29 December 2012

Technical and Other Jobs 3 in UAE
Published on Daily Express at 29 December 2012

Technical and Other Jobs 2 in UAE
Published on Daily Express at 29 December 2012

Technical and Other Jobs 1 in UAE
Published on Daily Express at 29 December 2012

Technical and Other Jobs 1 in Abu Dhabi
Published on Daily Express at 29 December 2012

Offices, Computer Assistant, Stenotypists, Library Assistant, Drivers, Naib Qasid and Other Jobs in National Saving Department Islamabad
Published on Daily Express at 29 December 2012

Directors, Engineers, Photographers, UDC, LDC, Cashiers, Accounts Clerk, Supervisors and Lot of Other Jobs in Government of Sindh Culture Department
Published on Daily Jang at 29 December 2012

Different Jobs 1 in Karachi and Adjacent Cities
Published on Daily Jang at 29 December 2012

Different Jobs 2 in Lahore and Adjacent Cities
Published on Daily Jang at 29 December 2012

Different Jobs 1 in Lahore and Adjacent Cities
Published on Daily Jang at 29 December 2012

Junior Assistants (Admin) Jobs in Public Sector Organization
Published on Daily Jang at 29 December 2012

Different Jobs 1 in Islamabad and Adjacent Cities
Published on Daily Jang at 29 December 2012

Principal, Admin Officers and Maintenane Supervisors Jobs in Leeds College Peshwar
Published on Daily Aaj at 28 December 2012

Computer Operators, Vocational Teachers, Security Staff and Lot of ther Jobs in Peshawar
Published on Daily Aaj at 28 December 2012

Technical and Other Jobs 1 in Malaysia
Published on Daily Aaj at 28 December 2012

Healthcare Staff, Junior Clerk, Junior Clinical Technicians and Lot of Other Jobs (Interview Schedule) in Executive District Officer Health Peshawar
Published on Daily Aaj at 28 December 2012

Junior Clerk and Security Staff Jobs in Swat Forest Division
Published on Daily Aaj at 28 December 2012

Directors, Engineers, Accountants, Computer Operators and Lot of Other Jobs in Government of Sindh Culture Department
Published on Daily Dawn at 28 December 2012

Drivers Jobs in Karachi Shipyard and Engineering Works Limited
Published on Daily Dawn at 28 December 2012

Security Staff Jobs in Public Sector Organization
Published on Daily Dawn at 28 December 2012

Scientists, Principal Scientific Officers, Junior Executives and Lot of Other Jobs in Global Change Impact Studies Centre (GCISC) Islamabad
Published on The News at 28 December 2012

Revenue Assistants Jobs in Punjab Industrial Estates Development and Management Company Lahore
Published on The News at 28 December 2012

Field Engineers, Social Organizers and Other Jobs in United Nations Development programme (UNDP) Khyber Pakhtunkhwa
Published on The News at 28 December 2012

Civil Engineers Jobs in Nishat Hotels and Propeties Limited Lahore
Published on The Nation at 28 December 2012

Security and Lot of Other Jobs (Interview Schedule) in Allama Iqbal Medical College Lahore
Published on The Nation at 28 December 2012

Junior Clerk and Security Staff Jobs in Swat Forest Division
Published on Daily Mashriq at 28 December 2012

Computer Operators, Vocational Teachers, Security Staff and Lot of ther Jobs in Khyber Pakhtunkhwa
Published on Daily Mashriq at 28 December 2012

Engineers, Geologists, Analytical Chemists and Lot of Other Jobs in Directorate General Mines and Minerals Peshawar
Published on Daily Mashriq at 28 December 2012

Professors, Associate Professors, Assistant Professors, Lecturers of Administration, Engineering, Computer Science, Marketing and Lot of Other Jobs in Iqra National University (INU) Peshawar
Published on Daily Mashriq at 28 December 2012

Different Jobs 1 in In Different Cities Nationwide
Published on Daily Khabrain at 28 December 2012

Managers IT, Administrators, Admin and HR Managers, Security Staff and Lot of Other Jobs in Punjab Metrobus Authority Lahore
Published on Daily Nawa e Waqt at 28 December 2012

Drivers Jobs in Karachi Shipyard and Engineering Works Limited
Published on Daily Express at 28 December 2012

Different Jobs 1 in Lahore and Adjacent Cities
Published on Daily Express at 28 December 2012

Technical and Other Jobs 4 in Saudia Arabia
Published on Daily Express at 28 December 2012

Technical and Other Jobs 4 in UAE
Published on Daily Express at 28 December 2012

Technical and Other Jobs 3 in Saudia Arabia
Published on Daily Express at 28 December 2012

Technical and Other Jobs 2 in Abu Dhabi
Published on Daily Express at 28 December 2012

Civil Engineers Jobs in Nishat Hotels and Propeties Limited Lahore
Published on Daily Express at 28 December 2012

Different Jobs 1 in Islamabad and Adjacent Cities
Published on Daily Express at 28 December 2012

Technical and Other Jobs 3 in UAE
Published on Daily Express at 28 December 2012

Technical and Other Jobs 2 in Saudia Arabia
Published on Daily Express at 28 December 2012

Technical and Other Jobs 1 in Abu Dhabi
Published on Daily Express at 28 December 2012

Academic Head, Teachers, Sales Directors, Marketing Managers, oordinators and Lot of Other Jobs in Dr A Q School System Islamabad
Published on Daily Express at 28 December 2012

Technical and Other Jobs 2 in UAE
Published on Daily Express at 28 December 2012

Technical and Other Jobs 2 in Libya
Published on Daily Express at 28 December 2012

Technical and Other Jobs 2 in Malaysia
Published on Daily Express at 28 December 2012

Technical and Other Jobs 1 in Saudia Arabia
Published on Daily Express at 28 December 2012

Technical and Other Jobs 1 in UAE
Published on Daily Express at 28 December 2012

Technical and Other Jobs 1 in Libya
Published on Daily Express at 28 December 2012

Technical and Other Jobs 1 in Malaysia
Published on Daily Express at 28 December 2012

Excise and Taxation Officers Jobs in Excise and Taxation Department Karachi
Published on Daily Jang at 28 December 2012

Accountants (Walk in Interview) Jobs in Government of Sindh Auqaf, Religious Affairs, Zakat and Ushr Department Karachi
Published on Daily Jang at 28 December 2012

Different Jobs 1 in Karachi and Adjacent Cities
Published on Daily Jang at 28 December 2012

Different Jobs 3 in Lahore and Adjacent Cities
Published on Daily Jang at 28 December 2012

Different Jobs 2 in Lahore and Adjacent Cities
Published on Daily Jang at 28 December 2012

Different Jobs 1 in Lahore and Adjacent Cities
Published on Daily Jang at 28 December 2012

Drivers MT and Sanitary Workers Jobs in Army Veterinary School Sargodha
Published on Daily Jang at 28 December 2012

Technical and Other Jobs 2 in UAE
Published on Daily Jang at 28 December 2012

Civil Engineers Jobs in Nishat Hotels and Propeties Limited Lahore
Published on Daily Jang at 28 December 2012

Different Jobs 1 in Islamabad and Adjacent Cities
Published on Daily Jang at 28 December 2012

Technical and Other Jobs 1 in UAE
Published on Daily Jang at 28 December 2012

Academic Head, Teachers, Sales Directors, Marketing Managers, oordinators and Lot of Other Jobs in Dr A Q School System Islamabad
Published on Daily Jang at 28 December 2012

Computer Opeators, Lab Technicians, ECG Technicians, OT Technicians and Lot of Other Jobs in Kashmor
Published on Daily Dawn at 27 December 2012

Electrical Engineers, Trainee Engineers, HVAC Engineers, Electronic Engineers and Other Jobs in International Center for Chemical and Biological Sciences University of Karachi
Published on Daily Dawn at 27 December 2012

Accountants Jobs in Government of Sindh Auqaf, Religious Affairs, Zakat and Ushr Department Karachi
Published on Daily Dawn at 27 December 2012

Electricians, Plumbers, Cook, Mali, Security Staff and Other Jobs in The High Court of Sindh, Karachi
Published on Daily Dawn at 27 December 2012

Drillers, Rig Mechanics, Assistants, Helpers and Other Jobs in UCG Project Thar
Published on The News at 27 December 2012

Provincial Coordinators Jobs in Balochistan
Published on The News at 27 December 2012

Monitoring, Evaluation and Research Coordinatos Jobs in Concern World Wide
Published on The News at 27 December 2012

Different Jobs 1 in Karachi and Adjacent Cities
Published on Daily Express at 27 December 2012

Excise and Taxation Officers Jobs in Excise and Taxation Department Karachi
Published on Daily Express at 27 December 2012

Different Jobs 1 in Lahore and Adjacent Cities
Published on Daily Express at 27 December 2012

Technical and Other Jobs 6 in UAE
Published on Daily Express at 27 December 2012

Technical and Other Jobs 5 in UAE
Published on Daily Express at 27 December 2012

Technical and Other Jobs 6 in Saudia Arabia
Published on Daily Express at 27 December 2012

Technical and Other Jobs 4 in UAE
Published on Daily Express at 27 December 2012

Technical and Other Jobs 5 in Saudia Arabia
Published on Daily Express at 27 December 2012

Technical and Other Jobs 3 in UAE
Published on Daily Express at 27 December 2012

Technical and Other Jobs 4 in Saudia Arabia
Published on Daily Express at 27 December 2012

Technical and Other Jobs 4 in Abu Dhabi
Published on Daily Express at 27 December 2012

Technical and Other Jobs 1 in Libya
Published on Daily Express at 27 December 2012

Technical and Other Jobs 2 in UAE
Published on Daily Express at 27 December 2012

Technical and Other Jobs 3 in Saudia Arabia
Published on Daily Express at 27 December 2012

Technical and Other Jobs 3 in Abu Dhabi
Published on Daily Express at 27 December 2012

Technical and Other Jobs 1 in UAE
Published on Daily Express at 27 December 2012

Technical and Other Jobs 2 in Saudia Arabia
Published on Daily Express at 27 December 2012

Technical and Other Jobs 2 in Abu Dhabi
Published on Daily Express at 27 December 2012

Technical and Other Jobs 1 in Abu Dhabi
Published on Daily Express at 27 December 2012

Technical and Other Jobs 1 in Saudia Arabia
Published on Daily Express at 27 December 2012

Scientists, Principal Scientific Officers, Junior Executives and Lot of Other Jobs in Global Change Impact Studies Centre (GCISC) Islamabad
Published on Daily Express at 27 December 2012

Electrical Engineers, Trainee Engineers, HVAC Engineers, Electronic Engineers and Other Jobs in International Center for Chemical and Biological Sciences University of Karachi
Published on Daily Jang at 27 December 2012

Assistant, Helpers and Other Jobs in UCG Project Sindh
Published on Daily Jang at 27 December 2012

Different Jobs 1 in Karachi and Adjacent Cities
Published on Daily Jang at 27 December 2012

Administrative Officers, Accountants, Computer Operators, Teaching Assistants, Security Assistants and Lot of Other Jobs in University of Sindh Jamshoro
Published on Daily Jang at 27 December 2012

Different Jobs 3 in Lahore and Adjacent Cities
Published on Daily Jang at 27 December 2012

Different Jobs 2 in Lahore and Adjacent Cities
Published on Daily Jang at 27 December 2012

Different Jobs 1 in Lahore and Adjacent Cities
Published on Daily Jang at 27 December 2012

Different Jobs 2 in Islamabad and Adjacent Cities
Published on Daily Jang at 27 December 2012

Different Jobs 1 in Islamabad and Adjacent Cities
Published on Daily Jang at 27 December 2012

Provicial Coordinators Jobs in Balochistan
Published on Daily Jang at 27 December 2012

Maid Jobs in Rawalpindi
Published on Daily Jang at 27 December 2012

Sales and Marketing Jobs in Rawalpindi
Published on Daily Jang at 27 December 2012

Principal Jobs in Cheema Trust Public School Rawalpindi
Published on Daily Jang at 27 December 2012

Primary Teachers Jobs in Cheema Trust Public School Rawalpindi
Published on Daily Jang at 27 December 2012

Chief Regulatory Officers Jobs in Karachi Stock Exchange Engineering Limited Karachi
Published on Daily Dawn at 26 December 2012

Different Jobs 1 in In Different Cities Nationwide
Published on The News at 26 December 2012

Security Guards Jobs in Phoenix Amour (Pvt) Limited Peshawar
Published on Daily Mashriq at 26 December 2012

Different Jobs 1 in Lahore and Adjacent Cities
Published on Daily Express at 26 December 2012

Technical and Other Jobs 4 in Saudia Arabia
Published on Daily Express at 26 December 2012

Technical and Other Jobs 10 in UAE
Published on Daily Express at 26 December 2012

Technical and Other Jobs 9 in UAE
Published on Daily Express at 26 December 2012

Technical and Other Jobs 2 in Oman
Published on Daily Express at 26 December 2012

Technical and Other Jobs 8 in UAE
Published on Daily Express at 26 December 2012

Technical and Other Jobs 3 in Saudia Arabia
Published on Daily Express at 26 December 2012

Technical and Other Jobs 1 in Abu Dhabi
Published on Daily Express at 26 December 2012

Technical and Other Jobs 7 in UAE
Published on Daily Express at 26 December 2012

Technical and Other Jobs 6 in UAE
Published on Daily Express at 26 December 2012

Technical and Other Jobs 5 in UAE
Published on Daily Express at 26 December 2012

Technical and Other Jobs 4 in UAE
Published on Daily Express at 26 December 2012

Technical and Other Jobs 2 in Saudia Arabia
Published on Daily Express at 26 December 2012

Technical and Other Jobs 1 in Saudia Arabia
Published on Daily Express at 26 December 2012

Technical and Other Jobs 3 in UAE
Published on Daily Express at 26 December 2012

Sales Managers, Sales Supervisors and Sales Man Jobs in Abroad / Middle East
Published on Daily Express at 26 December 2012

Technical and Other Jobs 2 in UAE
Published on Daily Express at 26 December 2012

Technical and Other Jobs 1 in UAE
Published on Daily Express at 26 December 2012

Technical and Other Jobs 1 in Oman
Published on Daily Express at 26 December 2012

Electricians, Plumbers, Cook, Mali, Security Staff and Other Jobs in The High Court of Sindh, Karachi
Published on Daily Jang at 26 December 2012

Superintendent Engineers and Other Different Jobs Jobs Walk in Interview in Public Health Engineering Department Karachi
Published on Daily Jang at 26 December 2012

Nurses Staff Jobs in Sindh Public Service Commision (SPSC) Karachi
Published on Daily Jang at 26 December 2012

Different Jobs 2 in Karachi and Adjacent Cities
Published on Daily Jang at 26 December 2012

Different Jobs 1 in Karachi and Adjacent Cities
Published on Daily Jang at 26 December 2012

Different Jobs 2 in Lahore and Adjacent Cities
Published on Daily Jang at 26 December 2012

Different Jobs 1 in Lahore and Adjacent Cities
Published on Daily Jang at 26 December 2012

Finance, Accounts, Sales and Marketing Managers and Executives Jobs in Oulin Pakistan (Pvt) Limited Lahore
Published on Daily Jang at 26 December 2012

Engineers, Site Inspectors, Stenographers, Surveyors and Other Jobs in Republic Engineering Corporation (Pvt) Limited Lahore
Published on Daily Jang at 26 December 2012

Different Jobs 2 in Islamabad and Adjacent Cities
Published on Daily Jang at 26 December 2012

Different Jobs 1 in Islamabad and Adjacent Cities
Published on Daily Jang at 26 December 2012

Senior Information Security Specialists and Information Security Experts Jobs in Department of The Auditor General of Pakistan Islamabad
Published on Daily Dawn at 25 December 2012

Software Developers, Web Designers, and Share Point Designers Jobs in Nexus Technologies Karachi and Islamabad Office
Published on The News at 25 December 2012

Managers and Medical Staff Jobs in Public Sector Organization
Published on The News at 25 December 2012

Superintending Engineers and Other Jobs in Public Health Engineering Department Sindh
Published on The News at 25 December 2012

Software Developers, Web Designers, and Share Point Designers Jobs in Nexus Technologies Karachi and Islamabad Office
Published on The News at 25 December 2012

Managers and Medical Staff Jobs in Public Sector Organization
Published on The News at 25 December 2012

Vice President (Operations) Jobs in Zarai Taraqiati Bank Limited (ZTBL) Islamabad
Published on The Nation at 25 December 2012

Different Jobs 1 in In Different Cities Nationwide
Published on Daily Khabrain at 25 December 2012

Computer Operators, Lab Technicians, OT Techncians, ECG Technicians and Lot of Other Jobs in District Health Officers Kashmore
Published on Daily Express at 25 December 2012

Vice President (Operations) Jobs in Zarai Taraqiati Bank Limited (ZTBL) Islamabad
Published on Daily Nawa e Waqt at 25 December 2012

Different Jobs 1 in Lahore and Adjacent Cities
Published on Daily Express at 25 December 2012

Technical and Other Jobs 9 in UAE
Published on Daily Express at 25 December 2012

Technical and Other Jobs 9 in Saudia Arabia
Published on Daily Express at 25 December 2012

Technical and Other Jobs 8 in Saudia Arabia
Published on Daily Express at 25 December 2012

Technical and Other Jobs 8 in UAE
Published on Daily Express at 25 December 2012

Senior Information Security Specialists and Information Security Experts Jobs in Department of The Auditor General of Pakistan Islamabad
Published on Daily Express at 25 December 2012

Managers and Medical Staff Jobs in Public Sector Organization
Published on Daily Express at 25 December 2012

Technical and Other Jobs 7 in UAE
Published on Daily Express at 25 December 2012

Technical and Other Jobs 6 in UAE
Published on Daily Express at 25 December 2012

Technical and Other Jobs 7 in Saudia Arabia
Published on Daily Express at 25 December 2012

Technical and Other Jobs 3 in Abu Dhabi
Published on Daily Express at 25 December 2012

Technical and Other Jobs 5 in UAE
Published on Daily Express at 25 December 2012

Technical and Other Jobs 2 in Libya
Published on Daily Express at 25 December 2012

Technical and Other Jobs 4 in UAE
Published on Daily Express at 25 December 2012

Technical and Other Jobs 3 in UAE
Published on Daily Express at 25 December 2012

Technical and Other Jobs 2 in Abu Dhabi
Published on Daily Express at 25 December 2012

Technical and Other Jobs 2 in UAE
Published on Daily Express at 25 December 2012

Technical and Other Jobs 1 in Libya
Published on Daily Express at 25 December 2012

Technical and Other Jobs 1 in UAE
Published on Daily Express at 25 December 2012

Technical and Other Jobs 1 in Abu Dhabi
Published on Daily Express at 25 December 2012

Technical and Other Jobs 6 in Saudia Arabia
Published on Daily Express at 25 December 2012

Technical and Other Jobs 5 in Saudia Arabia
Published on Daily Express at 25 December 2012

Technical and Other Jobs 4 in Saudia Arabia
Published on Daily Express at 25 December 2012

Sales Officers and Salesman Jobs in Top Star International Comapany Lahore
Published on Daily Express at 25 December 2012

Technical and Other Jobs 3 in Saudia Arabia
Published on Daily Express at 25 December 2012

Technical and Other Jobs 2 in Saudia Arabia
Published on Daily Express at 25 December 2012

Technical and Other Jobs 1 in Saudia Arabia
Published on Daily Express at 25 December 2012

Different Jobs 2 in Karachi and Adjacent Cities
Published on Daily Jang at 25 December 2012

Different Jobs 1 in Karachi and Adjacent Cities
Published on Daily Jang at 25 December 2012

Accountant, UDC, IT, Management Sciences Faculty and Lot of Other Jobs in Nation Wide
Published on Daily Jang at 25 December 2012

Different Jobs 3 in Lahore and Adjacent Cities
Published on Daily Jang at 25 December 2012

Different Jobs 2 in Lahore and Adjacent Cities
Published on Daily Jang at 25 December 2012

Different Jobs 1 in Lahore and Adjacent Cities
Published on Daily Jang at 25 December 2012

Call Centre Agents Jobs in Telecom Organization Lahore
Published on Daily Jang at 25 December 2012

Foremen Plumbers, Foremen Electricians and Labours Jobs in Foric Consultants and Education Lahore
Published on Daily Jang at 25 December 2012

Engineers Jobs in Lahore
Published on Daily Jang at 25 December 2012

Healthcare and Other Jobs in Federal Government
Published on Daily Jang at 25 December 2012

Different Jobs 1 in Islamabad and Adjacent Cities
Published on Daily Jang at 25 December 2012

Montessori and Primary Classes Teachers Jobs in Islamabad
Published on Daily Jang at 25 December 2012


You can browse jobs from previous dates from all newspapers by selecting your required date and newspaper:
Click here to View All Old Jobs

Published on All Newspaper


--
You received this message because you are subscribed to the Google Groups "VU Computer Science" group.
To post to this group, send email to vu-computer-science@googlegroups.com.
To unsubscribe from this group, send email to vu-computer-science+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/vu-computer-science?hl=en.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS