Re: [DIYbio] Re: Surface Plasmon Resonance

Here is a good paper on SPR as a biosensor: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4481982/

Contingent to my application with this device is the ability to test tens of things at once or more, meaning I can't use a single or even small set of probes. Plus as you may know, it is virtually impossible to attach probes to a specific protein in a raw lysate without also tagging a million other things. But SPR is a great way to find specific things in complex solutions label free using antibodies, etc.

On Saturday, October 20, 2018 at 2:56:21 AM UTC-4, Skyler Gordon wrote:
I've never heard of SPR being used on liquids.

Usually SPR uses Infra-Red (IR-SPR) and anything that IR will bounce off of should give you a signal that represents that material. If your sample is negative without bonding and positive with, it sounds possible.

That being said, if you're bonding something to a molecule that will result in IR-SPR signal, youre really just adding a probe anyways... I'd suggest just using some sort of probe.

-SG
On Fri, Oct 19, 2018 at 3:18 PM Jacob Palumbo <palumb...@gmail.com> wrote:
Sorry to necrobump a clearly dead thread, but I am actually specifically in need of this literally right now. In regards to Josiah's question, I'll run validation tests (can test lysozyme and various cancer biomarkers) in exchange for the spec and design files.

Let me know ASAP, thanks.

On Wednesday, January 28, 2015 at 2:01:23 PM UTC-5, Josiah Zayner wrote:
If Daniel responds to this thread I have a question about the Kickstarter.

Have you done any experiments to validate your instrument by showing it provides measurements similar to what is seen for commercial grade instruments in literature?




On Friday, January 23, 2015 at 12:14:23 PM UTC-8, Daniel Glenn wrote:

I was wondering if their would be interest in an affordable surface plasmon resonance bio-assay sensor?


Surface plasmon resonance can be used as a method to detect antibody-antigen (and other) reactions directly with an optoelectronic sensor. Biacore and others make very expensive instruments that read 96-well plates, but I'm talking about something that reads samples one at a time and doesn't cost $33,000+.


It can be used to perform the same type of assays that occur in Western blot and ELISA without attaching tags, because the probe molecule is immobilized chemically on the surface of the optics with chemistry. Would those type of assays be useful to the general DIYbio community?


Do you think that there would be interest in such a device for the more general citizen-science community if test kits for things like Salmonella or Dengue that anyone with reasonable skills and training could use were developed?


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Re: [DIYbio] Re: Surface Plasmon Resonance

I've never heard of SPR being used on liquids.

Usually SPR uses Infra-Red (IR-SPR) and anything that IR will bounce off of should give you a signal that represents that material. If your sample is negative without bonding and positive with, it sounds possible.

That being said, if you're bonding something to a molecule that will result in IR-SPR signal, youre really just adding a probe anyways... I'd suggest just using some sort of probe.

-SG

On Fri, Oct 19, 2018 at 3:18 PM Jacob Palumbo <palumbojacob@gmail.com> wrote:
Sorry to necrobump a clearly dead thread, but I am actually specifically in need of this literally right now. In regards to Josiah's question, I'll run validation tests (can test lysozyme and various cancer biomarkers) in exchange for the spec and design files.

Let me know ASAP, thanks.

On Wednesday, January 28, 2015 at 2:01:23 PM UTC-5, Josiah Zayner wrote:
If Daniel responds to this thread I have a question about the Kickstarter.

Have you done any experiments to validate your instrument by showing it provides measurements similar to what is seen for commercial grade instruments in literature?




On Friday, January 23, 2015 at 12:14:23 PM UTC-8, Daniel Glenn wrote:

I was wondering if their would be interest in an affordable surface plasmon resonance bio-assay sensor?


Surface plasmon resonance can be used as a method to detect antibody-antigen (and other) reactions directly with an optoelectronic sensor. Biacore and others make very expensive instruments that read 96-well plates, but I'm talking about something that reads samples one at a time and doesn't cost $33,000+.


It can be used to perform the same type of assays that occur in Western blot and ELISA without attaching tags, because the probe molecule is immobilized chemically on the surface of the optics with chemistry. Would those type of assays be useful to the general DIYbio community?


Do you think that there would be interest in such a device for the more general citizen-science community if test kits for things like Salmonella or Dengue that anyone with reasonable skills and training could use were developed?


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