[DIYbio] Re: Non-Platinum Electrophoresis Electrodes That Don't Degrade

Hi all and sorry for resurrecting this old thread.

I have retrofitted some old electrophoresis units with stainless steel wire for electrodes and this works using TAE buffer for a while before the 
buffer gets an odd color (green).

However, I recently cam across a superior buffer containing LiAc and boric acid and this seems much more aggressive, the unit fills up with rust after one run.

Platinum wire is an obvious choice, but it is quite expensive. 

I came across something I think wasn't mentioned here: conductive brush wire (sold here).

This wire is probably used for brushes that can not be allowed to build up static electricity.

This wire seems to be made from some plastic with a carbon filler.

Could this be an option?

/bjorn




On Sunday, May 26, 2013 at 7:29:19 PM UTC+1, Josiah Zayner wrote:
I have been testing out a bunch of different metals and they either oxidize to death on the minutes timescale or in the case of stainless steel turn the buffer orange/brown.

Platinum wire is pretty expensive ~$1.00 cm^-1. Has anyone had luck with a cheaper metal at 100V or greater with TAE?

I have tried:
Nickel
Stainless Steel 430
Tinned Copper

Thinking of trying Titanium or Nickel Titanium (<= $4 meter^-1). Anyone ever tried these?


Thanks,
     Josiah Zayner


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[DIYbio] The thought emporium and plants

https://m.youtube.com/watch?v=JKz3t1I6MAk

The Thought emporium just released a new video on getting a cross section of plants for dissection.

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