[DIYbio] transcranial magnetic stimulation (TMS) circuit model

attached picture for the enjoyment of all.

approx 100 us pulse

RTFM

## Jonathan Cline
## jcline@ieee.org
## Mobile: +1-805-617-0223
########################


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[DIYbio] DIYbio events for the week of July 31

For last minute edits, please go to
https://diybio.org/2016/07/30/diybio-events-for-the-week-of-july-31/

Here are your DIYbio events for the week.

On Sunday, join Brooklyn as they process black-and-white film (remember that?) using beer and coffee, and Oakland continues their Biohack Academy series with a course on culturing, as well as continuing on their Open Insulin,  and outer space microfluidics projects, while Sunnyvale has teens showing other teens  some basics of biology and biotechnology

On Monday, Charlottesville begins a two-week synthetic biology summer camp, Oakland continues its Biohacking Academy with a course on plasmid design and online databases, as well as continuing their Real Vegan Cheese project in collaboration with Sunnyvale.

On Tuesday, Amsterdam changes the venue of their Open Wetlab to Red Light Pet Shop pop-up location to explore their bio bank,  Brooklyn has Oliver Medvedik, Ph.D. and Ellen Jorgensen Ph.D. teaching a course on designing guide RNAs for use in CRISPR, Buenos Aires, has a discussion by Gabriella Munguia on "bioimpresion" as an intersection of biology, 3-D printing, and art, Madison has a talk by Joshua Hyman on DNA sequencing, and Sunnyvale continues its plant bio research and quantum salinity detector projects.

On Wednesday, Amsterdam has a workshop exploring the superfood potential of their algae "pets",  Lausanne/Ranens has a various projects and organizations sharing their work,  Oakland continues with their fun fermentation and Open Insulin projects, and Sydney continues with their ethylene biosensor iGEM project.

On Thursday, Buenos Aires hosts Pablo de Soto to talk about all sorts of things involving international hactivism and drones, including citizen science radiation mapping, and Sunnyvale continues their bioprinter project.

On Friday Singapore meets to share some improvised lab equipment and experiments, and Sunnyvale continues discussions about biohacking and climate change.

Finally, on Saturday Baltimore has Lisa Schifele Ph.D.  teaching the Build-a-Gene series of courses, which include an introduction to CRISPR/ Cas9, Brooklyn has Oliver Medvedik, Ph.D.beginning a lab series on gene editing using CRISPR/ Cas9 (there is a prerequisite), and has Moira Williams  conducting a workshop on making art from mushrooms and lichen, Oakland continues their evolved bacterial sunscreen project, in collaboration with Sunnyvale.  Also on Saturday, Seattle has an introduction to light microscopy,  and Sunnyvale begins a 3- session in-depth lab and lecture series on CRISPR/ Cas9.

Sunday, July 31
Brooklyn, NY, USA
Oakland, CA, USA
Oakland, CA, USA
Oakland, CA, USA
Sunnyvale, CA, USA

Monday, August 1
Charlottesville, VA, USA
Oakland, CA, USA
Oakland, CA, USA
Sunnyvale, CA, USA

Tuesday, August 2
Amsterdam, NTL
Brooklyn, NY, USA
Buenos Aires, ARG
Madison, WI, USA
Sunnyvale, CA, USA
Sunnyvale, CA, USA

Wednesday, August 3
Amsterdam, NTL
Lausanne/Ranens, CH
Oakland, CA, USA
Oakland, CA, USA
Sydney, NSW, AUS

Thursday, August 4
Buenos Aires, ARG
Sunnyvale, CA, USA

Friday, August 5
Singapore
Sunnyvale, CA, USA

Saturday, August 6
Baltimore, MD, USA
Brooklyn, NY, USA
Brooklyn, NY, USA
Oakland, CA, USA
Seattle, WA, USA
Sunnyvale, CA, USA
Sunnyvale, CA, USA

Other events coming up

Sunday, August 7
Brooklyn, NY, USA
Melboune, VIC, AUS
Oakland, CA, USA
Oakland, CA, USA

Monday, August 8
Oakland, CA, USA

Tuesday, August 9
Amsterdam, NTL
Madison, WI, USA
Sunnyvale, CA, USA

Wednesday, August 10
Amsterdam, NTL
Brooklyn, NY, USA
Oakland, CA, USA
Oakland, CA, USA

Thursday, August 11
Sunnyvale, CA, USA
Toronto, ON, CAN

Saturday, August 13
Baltimore, MD, USA
Denver, CO, USA

Sunday, August 14
Oakland, CA, USA
Oakland, CA, USA

Monday, August 15
Brooklyn, NY, USA
Cambridge, UK
Oakland, CA, USA
Oakland, Ca, USA

Tuesday, August 16
Madison, WI, USA
Sunnyvale, CA, USA

Wednesday, August 17
Oakland, CA, USA
Oakland, CA, USA

Thursday, August 18
Camden, NJ, USA
Sunnyvale, CA, USA
Sydney, AUS

Saturday, August 20
Baltimore, MD, USA
Oakland, CA, USA
Sunnyvale, CA, USA

Sunday, August 21
Durham, NC, USA
Oakland, CA, USA
Oakland, CA, USA

Monday, August 22
Oakland, CA, USA
Madison, WI, USA

Tuesday, August 23
Madison, WI, USA

Wednesday, August 24
Oakland, CA, USA
Oakland, CA, USA
Sunnyvale, CA, USA

Thursday, August 25
Oakland, CA, USA
Sunnyvale, CA, USA

Saturday, August 27
Baltimore, MD, USA

Sunday, August 28
Bethesda, MD, USA
Melbourne, VIC, AUS
Oakland, CA, USA

Tuesday, August 30
Minneapolis, MN, USA
Sunnyvale, CA, USA

Wednesday, August 31
Oakland, CA, USA
Oakland, CA, USA
Sunnyvale, CA, USA

Waag Societies recent Bio-Ink Workshop held at their pop-up Microbial  Pet Store in the Red Light District of Amsterdam at the Gallery Schatjes







To see when the next event is in your area, check the full list of usual suspects. Don't see one near you? Why not have your own and get it posted here? Tell us about it by emailing events@diybio.org. It would be great to post some photos of your event somewhere. We'd love to see them. We do have some guidelines for what is posted, and are not capturing every event within the community.


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[DIYbio] Re: Primer/Oligo Synthesis Companies that sell to individuals for personal use??

Yes, I've used oligos from Macrogen several times, and I have not had any problems with them. I have only used primers with basic purification (desalting) though, which may include more molecules with misplaced nucleotides, so if you are concerned about the quality in the sense of all the oligo molecules having the correct sequence, you may wish to order a higher purifcation level. Macrogen offers MOPS, PAGE and HPLC purification, but not for the smallest amount (0.025 umoles). My suggestion would be to start with ordering 0.025 umoles desalted to start with, and only upgrade if you have any problems.

-JP  

lørdag 30. juli 2016 06.25.22 UTC+2 skrev Al.K.J følgende:
Have you ever used oligos ordered from here? I'm just curious what the quality of the DNA is.

- A

On Friday, July 29, 2016 at 3:18:40 AM UTC-4, jarlemag wrote:
If you are willing to pay for shipping from Korea, I can reccomend Macrogen inc (http://macrogen.com/eng/). They accept payment by credit card. There is a field for "institute/company" in their online ordering form, but you can write anything there.

-JP

 

fredag 29. juli 2016 02.30.57 UTC+2 skrev Al.K.J følgende:


I recently tried to place an order through IDT, however, they kept insisting that they required some kind of institution affiliation and that they do not ship to "non-organizational entities".

Does anybody know of any oligo/gene synthesis company that will provide DNA for personal use?

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[DIYbio] Re: Labs doing research on bioelectronics and bionanowires?

There's Yuri Gorby, currently at Rensselaer.

Patrik

On Thursday, July 28, 2016 at 5:53:10 PM UTC-7, Biotech Ryan wrote:
Hi Biohackers,

Long time no speak! I had a hopefully quick question, does anyone know of any labs doing interesting research into bioelectronics and grown bionanowires outside of Derek Lovely's lab? I've started to dive deeper into this space of growing classical circuit boards and want to explore the concept a little, any suggestions?

Best,
Ryan (the Sudo Room, CCL, BBL, IndieBio and few other things bioguy)

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[DIYbio] Re: Primer/Oligo Synthesis Companies that sell to individuals for personal use??

Have you ever used oligos ordered from here? I'm just curious what the quality of the DNA is.

- A

On Friday, July 29, 2016 at 3:18:40 AM UTC-4, jarlemag wrote:
If you are willing to pay for shipping from Korea, I can reccomend Macrogen inc (http://macrogen.com/eng/). They accept payment by credit card. There is a field for "institute/company" in their online ordering form, but you can write anything there.

-JP

 

fredag 29. juli 2016 02.30.57 UTC+2 skrev Al.K.J følgende:


I recently tried to place an order through IDT, however, they kept insisting that they required some kind of institution affiliation and that they do not ship to "non-organizational entities".

Does anybody know of any oligo/gene synthesis company that will provide DNA for personal use?

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Looking for Senior Software Engineer, Big Data / Kafka , WA (Local Preferred)

HI,

 

Please find the below position and respond back to me at praveen@sritechsolutions.com

 

Senior Software Engineer, Big Data / Kafka

Redmond, WA

Long term Contract

 

Local candidates are preferred

 

Need a Strong candidate with Big date Kafka

 

Job Description:

·         Build our big data platforms and infrastructure using your strong background in distributed systems and large scale storage systems.

·         Provide thought leadership for the MFS data platform team and collaborate with cross-functional engineering teams to streamline or improve adoption of our platform.

·         Create robust high-volume production applications, and develop prototypes quickly.

·         Manage individual projects priorities, deadlines and deliverables with your technical expertise.

·         Design, develop, test, deploy, maintain, and enhance software solutions.

·         Technical mentoring of other engineers within the software team.

 

Qualifications

·         Strong fundamentals: data structures, algorithms, databases.

·         5-8 years of programming experience in software programming using Java, SQL etc.

·         Industry experience with design and development of large scale distributed systems.

·         3+ years of experience in working with data: data management, data wrangling, data modeling and architecture.

·         Hands on experience with any Linux/Unix flavor.

·         Extensive knowledge and industry experience in storage and streaming technologies (HBase, Kafka, Storm, Spark etc.)

·         Ability to design and solve computational problems utilizing distributed Hadoop technologies (Map/Reduce, Yarn etc.)

·         Worked with technologies such as web servers (Tomcat/Apache/IIS), REST services, SOA.


Thanks and Best Regards,

 

Praveen Kumar

SRI Tech Solutions Inc.

praveen@sritechsolutions.com

(T) 1-813-423-6500 X Ext: 149 (M) 813-440-2778 (F) 813.423.6520

 

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Backfill position:-Backend Java developer with telecom :: Warren NJ & Alpharetta, GA || Face to Face

Dear Partner

Hope you are doing great!!

 Please go through the requirement and let me know if you have any consultant for the same position.

 

Title                 : Java Developer

Locations        : Warren NJ

Interview         : Immediate

 

Job Description:

Backend Java developers with Java and DevOPS tools. Agile, GIT, Continuous Integration Jenkins experience is required.

 

Roles and Responsibilities:

·         Responsible for defining, planning, and monitoring all application support activities for IT applications including trouble shooting, performance monitoring, recommendation of resolution, installation, training, and script/code writing.

·         Able to work independently and with a DevOps/Agile team as necessary.

·         Able to participate in all phases of software development from inception to implementation and support. Collaborates with developers and clients to develop precise business logic. Assist the programmer in interpreting and implementing departmental standards and guidelines. Explain logical and technical situations so that the most efficient approach is taken; also explain the reasoning being the specified solution. Develop new complex computer applications and enhance existing systems according to assigned project requests, in accordance with established departmental standards and guidelines.

·         Responsible for researching, analyzing, coding, and testing all changes, drawing on user and technical resources to successfully complete the requests.

·         B.S. or M.S. degree in Computer science or equivalent is a must.

·         5+ years of experience in programming with emphasis on industrial client/server applications is a must

·         Industrial thorough experience with the most of the following technologies is strongly required:

·         J2EE, EJB 3.0/3.1, JMS, XML

·         COBRA, Oracle DB, REST based web services

·         Scripting language such as BASH, Pearl, Awk, TCL

·         Agile development technology



Thanks & Regards,

Arpit Arora
Technology Resource Group Inc.
1700 Park St, Unit 212, and Naperville IL, 60563
Direct: 909-859-1312 | Office:408-709-1760 Ext : 9961
Fax: 408-884-2409
Arpit@tresourceinc.com | www.tresourceinc.com

 



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[DIYbio] Call for expression of interest for Marie Skłodowska-Curie Actions (IF) - 3D Bioprinting

Aloha,

we're looking for someone with a PhD or at least 4 years of research experience, who would apply for a MSCA fellowship as a post-doc student at irnas. We will be developing a DIY 3D Bioprinter and are looking for people with molecular/cell biology background. I know the MSCA is hard to get, but it's worth a try =) All info in the pdf =)

Boštjan

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[DIYbio] Re: Primer/Oligo Synthesis Companies that sell to individuals for personal use??

If you are willing to pay for shipping from Korea, I can reccomend Macrogen inc (http://macrogen.com/eng/). They accept payment by credit card. There is a field for "institute/company" in their online ordering form, but you can write anything there.

-JP

 

fredag 29. juli 2016 02.30.57 UTC+2 skrev Al.K.J følgende:


I recently tried to place an order through IDT, however, they kept insisting that they required some kind of institution affiliation and that they do not ship to "non-organizational entities".

Does anybody know of any oligo/gene synthesis company that will provide DNA for personal use?

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Re: [DIYbio] Primer/Oligo Synthesis Companies that sell to individuals for personal use??

In IndieBio EU team just graduated yesterday offering €19/750bp dsDNA, Helix.works... and they're selling Gene Synth on Amazon to everyone! :)

On 29 July 2016 00:45:22 IST, "Al.K.J" <akruswick@gmail.com> wrote:


I recently tried to place an order through IDT, however, they kept insisting that they required some kind of institution affiliation and that they do not ship to "non-organizational entities".

Does anybody know of any oligo/gene synthesis company that will provide DNA for personal use?


--
Sent from my Android device with K-9 Mail. Please excuse my brevity.

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[DIYbio] Re: Labs doing research on bioelectronics and bionanowires?

Thanks Gordana, I was thinking of the growable type, made with bacteria like geobacter but I appreciate the suggestion!

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Re: [DIYbio] Help Fix an Invitrogen Blue Light Transilluminator

Those resistors have never been populated in the first place. It's not a mistake, but a common practice to have "optional" components on the PCB, and decide if you actually want to put the components at the time of PCB assembly.

Sorry to say, but this is not the source of the problem.

On Fri, Jul 29, 2016 at 8:18 AM, Cory Tobin <cory.tobin@gmail.com> wrote:
Hi everyone,

A non-functional blue light transilluminator was donated to my lab.  It's an Invitrogen Safe Imager Blue Light Transilluminator 2.0.  I cracked it open and found it was missing 3 surface mount resistors.  Otherwise the device seems to be in great shape.

See the images here:
http://imgur.com/a/kfkmU

If anyone has one of these transilluminators and wouldn't mind opening it up, I could use the values of the 3 missing resistors shown in the first picture.  It's really easy to open.  There's 9 screws on the back.  Once those are out it comes apart in two pieces and the top of the board is visible.

Thanks in advance if you can help me out.

-Cory

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[DIYbio] Re: Labs doing research on bioelectronics and bionanowires?

Not sure if this is along the same lines (are you looking only for growable wires?) but it might help http://science.sciencemag.org/content/337/6102/1640.full . John Rogers (Uic) is the guy.
 

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[DIYbio] Re: Perth (Australia) Group

Hi Leo,

Maybe it's too strong to call each a diyBio/makerspace, but
At UWA
I'm running a small group of students who are working on building an diy automated incubator+microscope with the goal of monitoring cell growth in developmental and synthetic bio. I'm also trying to pull off some diy tissue decellularisation + monitoring with an optical coherence microscopy system.
There is always SymbioticA - quite known in the bioart space (see Nathan above).
UWA has a makerspace: http://www.web.uwa.edu.au/engage/innovation-quarter/current-prototypes#new_div_2830652
They are running a few bio related projects.
UWA is also ramping up bioprinting (less diy, more professional research)
Another student group is working on a project similar to the stanford foldscope: http://www.foldscope.com/ , going around schools.

The problem I see is that there is a disconnect between higher research and students, and students and 'citizen' scientists.
I'm trying to help solve this to some extent. This is part of the agenda of the new uwa bioengineering program, so there is hope.
I think a lot has to do with lack of awareness through social / web media. (I've applied for both free uwa and externally funded web space a few months ago, but it's taking quite a while)
Many of these projects have started only this year, with the exception of SymbioticA, so it will take some time to establish a good presence.

I know diyBio is supposed to be somewhat decentralised from university, but so far it's been much easier finding space, money and participants here.

BTW, there is an open day on the 14th Aug, if you want to check out all of these.

With biohack academy, it looks like it is quite intense and quite pricey (~4k euro + 500 per person). And it is held right in the middle of when I am supposed to submit my phd. 
I will apply for funding, hopefully to waive most of out of pocket expenses. Or alternatively, I'll see if we can do a 'lite' version.

Cheers,
Philip


On Thursday, 28 July 2016 19:17:14 UTC+8, Leo Mason wrote:
Hi philip.
I am definitely interested in a meetup soon and would also be super keen on a biohack academy workshop if we can get it happening in perth.

Interesting you say there are a few groups here as I've only found one at the artifactory and been to a single meeting.
Like you say it would be a good idea to all team up/collaborate as i dont think its very organised here yet.
Could you post links to the other diybio/maker spaces so i can check it out?
Cheers

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Re: [DIYbio] Help Fix an Invitrogen Blue Light Transilluminator

It doesn't appear to me that those resistors had ever been
placed/soldered in. This is quite common, for selling variable
products with the same circuit board... or for test/calibration
points, or for backup in case there is a hiccup in parts supply and an
alternative circuit needs to be enabled so products can keep shipping
regardless of supply fluctuations. These are usually called
'BOM_IGNORE' or 'DNI' (do not inventory). We do this a lot with
capacitors, because the parts we test are so new the exact
requirements aren't know (though simulations/modelling gets us close).

I'd check all the solder joints with a jewelers loupe or a microscope
if you can fit the board under it... or a magnifying glass. Definitely
check that IC near the resistors you mentioned... and the transistor
with the heatsink. Mechanically stressed components (metal leads were
not in a relaxed state when soldered) can lift off of solder pads or
cause cracks, heat gets things close to the melting point, so one bad
hot day with heavy use could have lifted a single pin or cracked some
joint because of thermal cycling and dissimilar temperature
coefficiencts.

Second easy thing to check would be the response of the capacitors...
a simple signal generator (just a square wave of half a volt will
often do for in-circuit testing) and oscilloscope can diagnose bad
caps in a second or two.
https://en.wikipedia.org/wiki/ESR_meter#Methods_of_ESR_measurement

pic of electrical setup
http://meettechniek.info/passive/cap-images/circuit-c-squarewave.gif

write-up
http://fullnet.com/~tomg/esrscope.htm

some folks get away using a multimeter instead of an oscilloscope...
but I like 'seeing' the 'truth'.




I picked this up for testing bad lab equipment power supplies,
specifically because it was battery-powered and thus would minimize
risk of damaging through some weird short-circuit with
high-voltage/high-power:
https://www.amazon.com/Seeedstudio-DSO-Nano-v3/dp/B00UJOA10G/

it did need some modification to work well (desoldered the internal
flash chip, soldered in a micro SD card, then re-programmed with the
BenF firmware which upgrades the signal trigger SIGNIFICANTLY), pics
here of hardware mods:
http://seeedstudio.com/forum/viewtopic.php?f=22&t=4678&sid=49b9dc37e79d270e93051a041406c271


On Thu, Jul 28, 2016 at 5:18 PM, Cory Tobin <cory.tobin@gmail.com> wrote:
> Hi everyone,
>
> A non-functional blue light transilluminator was donated to my lab. It's an
> Invitrogen Safe Imager Blue Light Transilluminator 2.0. I cracked it open
> and found it was missing 3 surface mount resistors. Otherwise the device
> seems to be in great shape.
>
> See the images here:
> http://imgur.com/a/kfkmU
>
> If anyone has one of these transilluminators and wouldn't mind opening it
> up, I could use the values of the 3 missing resistors shown in the first
> picture. It's really easy to open. There's 9 screws on the back. Once
> those are out it comes apart in two pieces and the top of the board is
> visible.
>
> Thanks in advance if you can help me out.
>
> -Cory
>
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[DIYbio] Freeside Atlanta - Getting rid of equipment we don't have room for, (liquid handling machine)

Hey All,

I'm from Freeside Atlanta,
We are trying to put together a biolab, we got a bunch of stuff donated and a lot of it doesn't fit in our lab area.

We have a liquid handling machine and a couple incubators we need to get rid of.

I put them on craigslist, but really we just need need the space one way or another.

Is there any place we should look to find a place where this extra stuff could be useful to someone.
Or is this machine too old to be useful?

Here is a link to the liquid handling machine.
https://atlanta.craigslist.org/atl/bfs/5677628287.html

Thanks,
Nathan

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Re: [DIYbio] Primer/Oligo Synthesis Companies that sell to individuals for personal use??

I actually don't know for a fact whether we do, but it's surely worth a shot. Try shooting an email to kevin(dot)holden(at)synthego(dot)com, tell him Rikke sent you.

Good luck!

On Jul 28, 2016 5:30 PM, "Al.K.J" <akruswick@gmail.com> wrote:


I recently tried to place an order through IDT, however, they kept insisting that they required some kind of institution affiliation and that they do not ship to "non-organizational entities".

Does anybody know of any oligo/gene synthesis company that will provide DNA for personal use?

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[DIYbio] Labs doing research on bioelectronics and bionanowires?

Hi Biohackers,

Long time no speak! I had a hopefully quick question, does anyone know of any labs doing interesting research into bioelectronics and grown bionanowires outside of Derek Lovely's lab? I've started to dive deeper into this space of growing classical circuit boards and want to explore the concept a little, any suggestions?

Best,
Ryan (the Sudo Room, CCL, BBL, IndieBio and few other things bioguy)

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[DIYbio] Help Fix an Invitrogen Blue Light Transilluminator

Hi everyone,

A non-functional blue light transilluminator was donated to my lab.  It's an Invitrogen Safe Imager Blue Light Transilluminator 2.0.  I cracked it open and found it was missing 3 surface mount resistors.  Otherwise the device seems to be in great shape.

See the images here:
http://imgur.com/a/kfkmU

If anyone has one of these transilluminators and wouldn't mind opening it up, I could use the values of the 3 missing resistors shown in the first picture.  It's really easy to open.  There's 9 screws on the back.  Once those are out it comes apart in two pieces and the top of the board is visible.

Thanks in advance if you can help me out.

-Cory

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[DIYbio] Primer/Oligo Synthesis Companies that sell to individuals for personal use??



I recently tried to place an order through IDT, however, they kept insisting that they required some kind of institution affiliation and that they do not ship to "non-organizational entities".

Does anybody know of any oligo/gene synthesis company that will provide DNA for personal use?

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Backfill position:-Backend Java developer with telecom :: Warren NJ & Alpharetta, GA || Face to Face

Dear Partner

Hope you are doing great!!

 Please go through the requirement and let me know if you have any consultant for the same position.

 

Title                 : Java Developer

Locations        : Warren NJ

Interview         : Immediate

 

Job Description:

Backend Java developers with Java and DevOPS tools. Agile, GIT, Continuous Integration Jenkins experience is required.

 

Roles and Responsibilities:

·         Responsible for defining, planning, and monitoring all application support activities for IT applications including trouble shooting, performance monitoring, recommendation of resolution, installation, training, and script/code writing.

·         Able to work independently and with a DevOps/Agile team as necessary.

·         Able to participate in all phases of software development from inception to implementation and support. Collaborates with developers and clients to develop precise business logic. Assist the programmer in interpreting and implementing departmental standards and guidelines. Explain logical and technical situations so that the most efficient approach is taken; also explain the reasoning being the specified solution. Develop new complex computer applications and enhance existing systems according to assigned project requests, in accordance with established departmental standards and guidelines.

·         Responsible for researching, analyzing, coding, and testing all changes, drawing on user and technical resources to successfully complete the requests.

·         B.S. or M.S. degree in Computer science or equivalent is a must.

·         5+ years of experience in programming with emphasis on industrial client/server applications is a must

·         Industrial thorough experience with the most of the following technologies is strongly required:

·         J2EE, EJB 3.0/3.1, JMS, XML

·         COBRA, Oracle DB, REST based web services

·         Scripting language such as BASH, Pearl, Awk, TCL

·         Agile development technology

 

Regards

 

Ajit Singh

Technology Resource Group INC.

1700 Park St, Unit 212,

Naperville IL, 60563

Phone: 408-709-1760 x 9863

Email : ajit.singh@tresourceinc.com

 

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