D@nc1ng B3ar - P@rty Anim@ls

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B1g T1ts, R0und Ass3s - C@mila

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Re: [DIYbio] Re: Help for thesis: american law regulating research

See in-line responses below.

On Fri, Jan 31, 2014 at 2:26 PM, Eni Idea <eni.idea4@gmail.com> wrote:
> Okay, but still I don't really get it...;)
> So, you say you can almost do anyhting you want ..you mean at home?

Yes, almost anything, except the exceptions. Because the government
has a strong State level presence, meaning sometimes State laws can
supersede Federal law, laws and their enforcement vary throughout the
country. Then there are counties, towns, villages, cities. All can
have laws, but generally these cannot supersede State or Federal (i.e.
reduce restrictions), only add restrictions.

This article looks pretty good upon first glance, zoning and
home-business are keywords here (just because :
"Is Your Home Zoned for Business?"
http://www.entrepreneur.com/article/68844

"Types of Zoning"
http://realestate.findlaw.com/land-use-laws/types-of-zoning.html


> One of
> the most important things is: is there, e. g., a special agency that really
> has control over your work?

Only if you start breaking laws do agencies get specific, or a certain
locality has a law saying you need to have permission. There are
Federal laws about Animal welfare, but even this depends on the
Zoning, i.e. if you're technically a farm things can be different.
This would be handled by the USDA, who also handles GMO plant stuff:
http://www.aphis.usda.gov/about_aphis/



> And are all guidelines (NIH; CDC; BMBL;) only recommendet or required if you
> don't research at an institution?

They are recommendations, period. Institutions follow them because
they're generally good recommendations, and they'll get sued for
negligence if something were to go wrong and they weren't following
industry-accepted best-practices that might have prevented the
accident.

The same would apply to an individual who was within their rights of
the law to perform experiments, if they screw up and were found to be
lazy with some safety mechanism, they'll likely be sued/jailed. Some
biotech/chemical companies break the law year after year, but simply
pay the fine since their profit is large enough. Sometimes these
companies eventually get taken to court to face shutdown if their
practices don't change, or they jail the CEO or whoever blame falls to
legally.

OSHA would also be a good research topic for you, but I think this
might only apply if you're paying someone to work:
https://en.wikipedia.org/wiki/Occupational_Safety_and_Health_Administration#Health_and_safety_standards

> So let's say you want to establish a community lab, can you open a BSL 1 -
> lab without any registration and expertise or is there an agency for the
> autorisation process?

Maybe, a knowledgeable 'Agent' could be a local lawyer, or as the
entrepreneur.com article says "The first step is to call or visit your
local municipal building and ask to see copies of the ordinances that
apply to home occupations". (or other home/residential ordinances)

> Another question: you mentioned city laws, are there really special laws for
> each bigger city and if so, what are they mostly about?

Yes, they vary by city and by region. The range of topics varies too,
anti-cloning, anti-guns, anti-explosives, anti-GMO, anti-nuclear. If
some group of people can convince the local population and achieve
majority vote, most anything can become a law. Some places pay you to
install rain-water capture barrels, others make it illegal, and it
depends on the property's zoning and extent of development:
http://blog.heritage.org/2012/08/07/when-it-rains-in-oregon-the-state-owns-the-raindrops/
www.naturalnews.com/029286_rainwater_collection_water.html

"Oregon graciously allows Oregonians to collect water from artificial
impervious surfaces (rooftops, parking lots, etc.) and feed it
directly into rain barrels."
"But if that water touches the ground, it is the property of the state
of Oregon, and you cannot collect it without a permit. Since Oregon
owns it, using it without permission would be a form of stealing."




See this *great* overview by Raymond:
http://chimera.labs.oreilly.com/books/1234000001995/ch07.html

"
Even in the US, there is a patchwork of regulations dependent on where
you live, although this is the exception rather than the rule. In
California, at the state level, human reproductive cloning is banned,
with a fine of $1 million levied for violations, making it
prohibitively expensive to raise a large clone army in the Golden
State. (Protip: Nevada does not even prohibit the use of state funds
for human cloning, borders California, and the available huge tracts
of desert land may be more suitable for a secret underground lair.)
Note that most of these laws have monetary fines rather than criminal
penalties. A few federal regulations exist, but most of these also
lack criminal teeth. They use the carrot of federal funding to enforce
experimental standards or restrictions. Bans on funding for
reproductive human cloning and the reversed ban on using particular
cell lines for stem cell research are good examples of using funding
carrots to enforce limits on research.
"

"
What will probably have more impact on you is where you interface with
your local utilities and services infrastructure. Most local
governments (at the city, county, or water district level) have
regulations about what's OK to dump down the sewer or throw in the
trash. What is hazardous, and how do you dispose of it? The short
answer is: if you'd be uncomfortable spilling it on your dining room
table, don't just flush it or throw it away. Check with your local
water and trash authorities about the best way to proceed. For
instance, most water authorities require that residential wastewater
result from basic activities like washing and not contain hazardous
waste. Specific restrictions may include things like allowable range
of pH, anything that can catch on fire, materials that might cause
obstructions, and even the color of the water.
"
"
Finally, in the US, the last resort when people are hurt or property
is damaged is often hiring a lawyer and suing someone. If you burn
down your apartment building, it really doesn't matter if it's from a
lamp you badly rewired or if you left an alcohol lamp
burning--something you could have prevented burnt several homes to the
ground, and you may be liable.
"

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Re: [DIYbio] Help creating expert system content for diybio

The demo Q and A thing seems like it would be better made with
something that evolved from HyperCard... this online 'story writer'
program seems like it would be perfect for actually getting biologists
to write some great info into one of these things.

http://writer.inklestudios.com/stories/4vp5
http://writer.inklestudios.com/

On Fri, Jan 31, 2014 at 3:16 PM, William Heath <wgheath@gmail.com> wrote:
> Hi All,
>
> I have created an asrynchronous backward chaining rules engine html5 rich
> internet application that interprets prolog rules. I am using to create an
> expert system for diybio. It is fully opensource and I have the beginnings
> of the expert system here:
>
> http://plnkr.co/edit/yuZkr6VoKWswAp7KM3mt?p=preview
>
> If your not familiar with plnkr, you can just fork it and then save it to
> make changes. Check it out and add to it!
>
> -Tim
>
> P.S.
>
> Please share your additions with the community.
>
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> -- You received this message because you are subscribed to the Google Groups
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Re: [DIYbio] Re: Dwarf tobacco?

On Fri, Jan 31, 2014 at 1:44 PM, Cathal Garvey
<cathalgarvey@cathalgarvey.me> wrote:
> I imagine a modified tobacco with pest or fungal resistance would be big
> money, but then you'd be one of the bad guys for helping make more
> cigarettes in the world. :)

'Better Cigarette' GMO? Throw some caffeine in there, pull in the
coffee market. Mellow Methyl Salicilate 'natural menthol' smokes, or
Intelligent Isoamyl Acetate (aka Bursting Banana) flavor. Some
biosynthetic combustion product that scrubs carcinogens from the
smoke.

This is fun!

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[DIYbio] Re: Plasmid miniprep kit

Syd Labs sells cheap spin columns for plasmid miniprep. http://www.sydlabs.com/spin-column-for-plasmid-miniprep-p110.htm

On Sunday, March 25, 2012 7:50:46 PM UTC-4, Tom Randall wrote:
I am putting together the reagants for a plasmid miniprep kit for myself and had a simple question if anybody out there has done this. Two of the solutions require guanidium HCl (http://www.roningenetics.org/Protocols.html, about 80% of the way down the page under the heading "Solutions for plasmid minipreps", buffer N3 and buffer PB, 4M and 5M guanidium HCl, respectively). Having always bought commercial kits before, I was wondering if these two solutions really needed autoclaving. I suspect not since guanidium is a strong protein denaturant so it seems highly unlikely anything, including DNases, would survive a 4 or 5M solution of this. I will test my kit without autoclaving first, but any suggestions would be welcome.

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[DIYbio] Help creating expert system content for diybio

Hi All,

I have created an asrynchronous backward chaining rules engine html5 rich internet application that interprets prolog rules.  I am using to create an expert system for diybio.  It is fully opensource and I have the beginnings of the expert system here:


If your not familiar with plnkr, you can just fork it and then save it to make changes.  Check it out and add to it!

-Tim

P.S.

Please share your additions with the community.  

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Re: [DIYbio] Re: Help for thesis: american law regulating research

> So let's say you want to establish a community lab, can you open a BSL 1 - lab without any registration and expertise or is there an agency for the autorisation process?

As far as I understood, yes you can. S1 means "genes and organisms that do not pose a risk to human health and the environment". Thus, why should it be forbidden?

We sholud de-regulate S1 too here in Europe.


On Fri, Jan 31, 2014 at 11:26 PM, Eni Idea <eni.idea4@gmail.com> wrote:
Okay, but still I don't really get it...;)
So, you say you can almost do anyhting you want ..you mean at home? One of the most important things is: is there, e. g., a special agency that really has control over your work?
And are all guidelines (NIH; CDC; BMBL;) only recommendet or required if you don't research at an institution?
So let's say you want to establish a community lab, can you open a BSL 1 - lab without any registration and expertise or is there an agency for the autorisation process?
Another question: you mentioned city laws, are there really special laws for each bigger city and if so, what are they mostly about?

Thanks again, it's not that easy to understand the American system, because in Austria, as already mentioned, you got really strict regulations. So here it's clear that you need to register somewhere if you want to organize community research...
Greetz, Eni

Am Samstag, 25. Januar 2014 22:01:12 UTC+1 schrieb Jacob:
In the US BS levels are guidelines not specific regulations. 

So generally you can do anything, provided you don't fall afoul of another agencies specific regulations: 
  • OSHA(if you have employees)
  • EPA(environmental release)
  • CDC(when dealing with select agents)
  • NIH/NSF(only when receiving funding from those sources)
  • City Laws(Zoning issues if you don't live in Houston).
  • FDA/HHS(if you are doing something that requires an IRB ie human studies)
I much prefer this method, because adding a restriction across the board would just add a cost to independents and school labs, while not actually increasing safety. Since everyone mostly follows the CDCs guidelines even if they don't have to. And it's not the creation of a GMO in a lab that's potentially harmful but releasing said GMO which is already regulated by the EPA and/or the FDA.



On Sat, Jan 25, 2014 at 1:45 PM, Mega [Andreas Stuermer] <masters...@gmail.com> wrote:
So, my question: Are there any regulations in America or do you have to abide by any law? 

I just know that S1 is unregulated. So it seems to be an absence of regulation in this particular case. Rüdiger from Berlin mentioned there even is a German law granting freedom of doing science, Recht auf freie Wissenschaft (or something like that). 


As you sure know, this right  stops with genetic modification here in Austria as well as in Germany. 

 suppose that it might be important if you want to establish a community lab or if you need materials for experiments. Or even if you tinker at home, isn't there any limiting rule??

AFAIK, you can do anything as long as it is not S2  regulated. 


The total American law system is totally diferent from ours. while we have the precautionary principle - everything is forbidden that is not allowed, in the US anything is allowed as long as not proven dangerous (evidence based) 


Greetings from Oberösterreich,
Andreas

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Re: [DIYbio] Re: Help for thesis: american law regulating research

Okay, but still I don't really get it...;)
So, you say you can almost do anyhting you want ..you mean at home? One of the most important things is: is there, e. g., a special agency that really has control over your work?
And are all guidelines (NIH; CDC; BMBL;) only recommendet or required if you don't research at an institution?
So let's say you want to establish a community lab, can you open a BSL 1 - lab without any registration and expertise or is there an agency for the autorisation process?
Another question: you mentioned city laws, are there really special laws for each bigger city and if so, what are they mostly about?

Thanks again, it's not that easy to understand the American system, because in Austria, as already mentioned, you got really strict regulations. So here it's clear that you need to register somewhere if you want to organize community research...
Greetz, Eni

Am Samstag, 25. Januar 2014 22:01:12 UTC+1 schrieb Jacob:
In the US BS levels are guidelines not specific regulations. 

So generally you can do anything, provided you don't fall afoul of another agencies specific regulations: 
  • OSHA(if you have employees)
  • EPA(environmental release)
  • CDC(when dealing with select agents)
  • NIH/NSF(only when receiving funding from those sources)
  • City Laws(Zoning issues if you don't live in Houston).
  • FDA/HHS(if you are doing something that requires an IRB ie human studies)
I much prefer this method, because adding a restriction across the board would just add a cost to independents and school labs, while not actually increasing safety. Since everyone mostly follows the CDCs guidelines even if they don't have to. And it's not the creation of a GMO in a lab that's potentially harmful but releasing said GMO which is already regulated by the EPA and/or the FDA.



On Sat, Jan 25, 2014 at 1:45 PM, Mega [Andreas Stuermer] <masters...@gmail.com> wrote:
So, my question: Are there any regulations in America or do you have to abide by any law? 

I just know that S1 is unregulated. So it seems to be an absence of regulation in this particular case. Rüdiger from Berlin mentioned there even is a German law granting freedom of doing science, Recht auf freie Wissenschaft (or something like that). 


As you sure know, this right  stops with genetic modification here in Austria as well as in Germany. 

 suppose that it might be important if you want to establish a community lab or if you need materials for experiments. Or even if you tinker at home, isn't there any limiting rule??

AFAIK, you can do anything as long as it is not S2  regulated. 


The total American law system is totally diferent from ours. while we have the precautionary principle - everything is forbidden that is not allowed, in the US anything is allowed as long as not proven dangerous (evidence based) 


Greetings from Oberösterreich,
Andreas

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DIRECT CLIENT REQUIREMENT FOR ETL DEVELOPER IN NY

Hello,

Hope you are doing well
Please go through the following requirement and please let me know on
recruiter@tcagroupmail.com if you have any available candidates

 

Role : ETL Developer
Location : Brooklyn, NY

 

SCOPE OF SERVICES

 

The scope of services to be performed by the selected individual includes:

·         Work closely under the direction of the Project Manager with stakeholders from Housing Recovery Office, OMB, DoITT, the Mayor’s Office and other stakeholders including managers, line employees, and IT staff.

·         Responsible for designing and maintain ETL code using Informatica

·         Work with the Team Lead to ensure that the right solution is put in place as per design

·         Should be able to understand and follow the technical design documents and update them, if needed, during the development phase.

·         Execute development plans and revise as appropriate to meet changing needs and requirements.

·         Modify existing programs to confirm to system changes or to make improvements in the existing program.

·         Write and maintain documentation to describe program development, logic, coding, testing changes and corrections.

 

MANDATORY SKILLS / EXPERIENCE:

 

·         At least 4 years of overall development experience

·         Must have 3+ years of ETL experience and Business Intelligence experience.

·         Must have Informatica PowerCenter development experience

·         Must have Informatica B2B Data Transformation development experience

·         Ability to Design, Develop and Deploy the ETL processes and performance tune ETL        programs/scripts

·         Strong knowledge of XML, XML Schema, XSD, XSLT/XPath and BI and Data Warehousing concepts

·         Strong experience in Oracle, PL/SQL

·         Unix shell scripting

·         Must be able to see tasks through to completion without significant guidance.

·         Must be able to provide documentation on their work.

 

DESIRABLE SKILLS/EXPERIENCE :

 

·         A Bachelors or Masters degree in MIS, Computer Science, or equivalent experience is desired;

·         Knowledge of Java/J2EE is a plus

·         Knowledge of Jasper Reports, OBIEE

·         Experience with formal SDLCs and business analysis methodologies;

·         Working knowledge of business analysis documentation standards;

Thanks,
Shalin
TCA CONSULTING GROUP
recruiter@tcagroupmail.com
908-448-0874

 

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Re: [DIYbio] Re: Dwarf tobacco?

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I imagine a modified tobacco with pest or fungal resistance would be big
money, but then you'd be one of the bad guys for helping make more
cigarettes in the world. :)

On 31/01/14 21:35, Nathan McCorkle wrote:
> On Fri, Jan 31, 2014 at 1:21 PM, Koeng <koeng101@gmail.com> wrote:
>> Any particular reason you like tobacco over arabidopis? Sorry, I am not
>> really into plant biotech so I don't know a lot of the differences
>
> Maybe because it's a commercial crop more data exists? More commercial
> value if the inventor comes up with some awesome GMO?
>

--
Please help support my crowdfunding campaign, IndieBB: Currently at
18.3% of funding goal, with 41 days left:
http://igg.me/at/yourfirstgmo/x/4252296
T: @onetruecathal, @IndieBBDNA
P: +3538763663185
W: http://indiebiotech.com

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Re: [DIYbio] Re: Dwarf tobacco?

On Fri, Jan 31, 2014 at 1:21 PM, Koeng <koeng101@gmail.com> wrote:
> Any particular reason you like tobacco over arabidopis? Sorry, I am not
> really into plant biotech so I don't know a lot of the differences

Maybe because it's a commercial crop more data exists? More commercial
value if the inventor comes up with some awesome GMO?

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Re: [DIYbio] Re: Primer Software?

Invitrogen value oligos ftw

I think its true that snapgene is trying to be the jack of all trades... I wish there was opensource software (hint hint) just like theirs so you could program your own plugins. So far all the free ones i have tried don't even marginally compare to it. On the topic of primers, I wish they added more support in for primers... last time I asked about it they gave this, which was pretty good explanation

-Koeng 

 


On Friday, January 31, 2014 8:37:58 AM UTC-8, Sebastian wrote:
I have the licensed version for my company but I really don't trust the primer Tm calculation since it does not use the "latest" calculation methods. If you take your primer made via snapgene and plug it into a more sophisticated program like Primer 3 Plus, which checks for hairpins, self annealing, and a ton of other user editable parameters, the Tm will vary from program to program. I've had issues designing primers via snapgene's minimalistic calculation approach. A few of my longer primers ~40bp failed. Later analysis with Primer 3 Plus showed a high chance of hairpin. When I redesigned it using only primer 3 it worked like a charm. I absolutely LOVE SnapGene but its trying to be jack of all trades. Even though I get my primers for a flat $5 a pop, its still a waste to have them fail due to silly avoidable design flaws...partly my own fault :P

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D

From: Koeng
Sent: 1/31/2014 11:27 AM
To: diy...@googlegroups.com
Subject: [DIYbio] Re: Primer Software?

I use snapgenes built in software. Just outline dna and it gives TM. I highly recommend the trail version

On Friday, January 31, 2014 5:24:52 AM UTC-8, Sebastian wrote:
I use Primer 3 Plus. Its nice and simple. What are your favorites? Anyone ever try premier biosoft's program? Did anyone ever get Tm that's way off predicted? Just trying to get an idea of what everyone uses to see if im not missing out on some open source goodness. Thanks!

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D

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[DIYbio] Re: Dwarf tobacco?

Any particular reason you like tobacco over arabidopis? Sorry, I am not really into plant biotech so I don't know a lot of the differences

-Koeng

On Thursday, January 30, 2014 9:36:21 PM UTC-8, Sebastian wrote:
Does anyone have any dwarf tobacco seeds? Im growing some petite Havana but they are actually not so petite...my lab space is limited so any dwarf mutant if the Nicotiana genus would be quite welcome. Thanks!

PS I hate arabidopsis.

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D

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Re: [DIYbio] "Scientists in Japan showed stem cells can now be made quickly just by dipping blood cells into acid

This, if confirmed, it the news of the year!

It simply means stem cells for everyone!

On 31 Jan 2014, at 20:24, Cory Geesaman <cory@geesaman.com> wrote:


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[DIYbio] "Scientists in Japan showed stem cells can now be made quickly just by dipping blood cells into acid

That sounds feasible (and legal) to do in your basement.
Some time ago we talked that you shouldn't inject yourself your own stem cells. But would they really develop into cancer? their p53 and ras was not mutated so they should just remain stem cells. Or differentiate (more likely)

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[DIYbio] "Scientists in Japan showed stem cells can now be made quickly just by dipping blood cells into acid

http://www.bbc.co.uk/news/health-25917270

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RE: [DIYbio] Re: Primer Software?

I have the licensed version for my company but I really don't trust the primer Tm calculation since it does not use the "latest" calculation methods. If you take your primer made via snapgene and plug it into a more sophisticated program like Primer 3 Plus, which checks for hairpins, self annealing, and a ton of other user editable parameters, the Tm will vary from program to program. I've had issues designing primers via snapgene's minimalistic calculation approach. A few of my longer primers ~40bp failed. Later analysis with Primer 3 Plus showed a high chance of hairpin. When I redesigned it using only primer 3 it worked like a charm. I absolutely LOVE SnapGene but its trying to be jack of all trades. Even though I get my primers for a flat $5 a pop, its still a waste to have them fail due to silly avoidable design flaws...partly my own fault :P

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D

From: Koeng
Sent: 1/31/2014 11:27 AM
To: diybio@googlegroups.com
Subject: [DIYbio] Re: Primer Software?

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[DIYbio] Re: Primer Software?

I use snapgenes built in software. Just outline dna and it gives TM. I highly recommend the trail version

On Friday, January 31, 2014 5:24:52 AM UTC-8, Sebastian wrote:
I use Primer 3 Plus. Its nice and simple. What are your favorites? Anyone ever try premier biosoft's program? Did anyone ever get Tm that's way off predicted? Just trying to get an idea of what everyone uses to see if im not missing out on some open source goodness. Thanks!

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D

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[[K2S]]+[[UPS]] B@rely L3ga1 # 139

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[DIYbio] Primer Software?

I use Primer 3 Plus. Its nice and simple. What are your favorites? Anyone ever try premier biosoft's program? Did anyone ever get Tm that's way off predicted? Just trying to get an idea of what everyone uses to see if im not missing out on some open source goodness. Thanks!

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D

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[[K2S]]+[[UPS]] Everyth!ng 6utt - @na1 Expl0rin9 S3xy S1ut Gai@

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[[K2S]]+[[UPS]] TeenF!d3lity - Jilli@n Jan$on

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[DIYbio] Re: DIYBio crowdfunding campaign to conduct algae research for bioremediation and biopolymers

Hi,

I think this is an excellent project and I am happy to help if I can, but I also feel that currently it is not a good target for crowdfunding or DIYBio. Since its specific for the Salton sea, anyone not located there would have little interest in contributing to it.

However, I am certain that you could attract much attention locally: in LA or the bay area. Both capital and talented minds should be available there, but I am also certain that you are campaigning there already.

Maybe if you made a location independent, fun sideproject out of it, like the rainbow colored nannos or similar, that could get more support internationally.

On Monday, 9 December 2013 06:17:45 UTC+1, cameron wrote:
Can you help with a crowdfunding campaign for DIYbio research on algae bioremediation of the Salton Sea?

Friends,
As a member of the DIYBio community for a few years and an active member at Biocurious for the last two, I've been focusing part of my time on the development of opensource tools and protocols for algae research.  We are making slow but steady progress at Biocurious and we are publishing our information on our WIKI (also links to Google Group and other resources where we share information publicly).

Recently, a friend and I started a non-profit called Blue Nomad Foundation, which was spawned from the work at Biocurious.  Our mission is to fund research to develop technologies to use algae to bioremediate polluted water sources and use the biomass for biopolymer development and educate the public. Our plan is to fund the development of these technologies and make the IP freely available. 

We are putting together a crowdfunding campaign to raise money to fund initial research in DIYBio spaces.  We hope to provide resources for memberships, equipment and reagents to facilities which can define projects consistent with our mission.  As a passionate member of this community with a lot of time invested in it, I believe that demonstrating that we can work towards solving real global problems will go a long way towards allaying some of the public fears about our work.  Our goal is to raise $40k and gain visibility in the press and alert the public to this approach.   We also intend to raise more significant money from foundations down the road to fund projects with academic algae researchers.  At Biocurious, I've been working to integrate other projects with institutional labs and I think this effort with BNF can do the same. I'm very keen to open the pipes between institutions and DIYBio to created blended research projects (if you care to learn more, feel free to write me separately).

So, I'm seeking opinions from this community on ways that our non-profit can craft our crowdfunding campaign to be consistent with the community interests and help DIYBio achieve our goals.  FYI, we were thinking of ways that we can use the campaign to contribute more to the community.  One thought which Patrik D suggested was to hold a DIYBio camp down at the Salton Sea where we could go on site to do some extremophile bioprospecting and hold talks on bioremediation and research there.  We could also discuss our work at Biocurious on algae genetic engineering, the protocols we are using, and what we've learned to date.  FYI, the Salton Sea is where BNF will focus our initial algae bioremediation efforts and has a lot of interesting environmental challenges. 

Finally, I want to ensure that we reach our $40k threshold.  Do any of you have thoughts on how BNF could reach this goal so that we can give back to the community?  I'm open to all ideas!

Thanks!
Cameron 

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[k2s][ups] Bl@ck G1rlfri3nd S3x T@pes # 2 ++

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[k2s][ups] Eur0S3xP@rties - Marina Visc0nti ++

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Re: [DIYbio] Dwarf tobacco?

How big is too big? I have some tobacco that gets about 1 to 1.5 feet
tall. I'm not sure if it's actually a dwarf variety or if I'm just
stunting the growth by planting in small pots. But even if I am just
stunting it, it's able to flower and produce viable seeds.

-cory

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