[DIYbio] Iranian Engineers Develop $99 Open Source Ventilator

"This project is licensed under the MIT License"

STM32F407 Disco board Open Ventilator Presentation/Demo https://www.youtube.com/watch?v=p47dtsTrSBI

"Iranian Engineers Develop Open Source Ventilator, IEEE Senior Member Hadi Moradi is leading the group" https://spectrum.ieee.org/the-institute/ieee-member-news/engineers-iran-open-source-ventilator

"Hadi Moradi, an associate professor at the University of Tehran's School of Electrical & Computer Engineering, is working to develop an open source ventilator for patients who have COVID-19. The IEEE senior member and his team have designed a low-cost and easy-to-build ventilator and plan to make its specifications available to manufacturing companies in Iran.   "Due to the current healthcare situation and the fact that the initial design previously had been tested by the MIT team, the ministry allowed us to test our first version during the week of 5 April. It was important to put the device on the right class of equipment so the required standards can be met accordingly.  We hope to finish our initial testing the week of 12 April and start production locally the following week.  We are aiming to make the design open source and will let other IEEE regions know. Since building this machine involves a combination of electrical, mechanical, and bioengineering, it is important to have members from all these field work together to build it." "


"Download me here:
https://github.com/stm32ov/ov​
Our goal is to provide a simple solution to build a low cost yet effective emergency ventilator.
This design is appealing because of the redundancy of the servos, which combined with current monitoring as well as pressure and airflow control provides control similar to what is found in high end ventilators. Peek can be provided by using back-pressure on the output line.
Our main focus is manufacturing the shield and control unit box, while the other parts would be printed or sourced locally. However, if necessary, get in touch with us and we'll be able to provide the whole system fully assembled.
The parts have been chosen carefully in order to make sure that there will be no stock supply issues up to 1000 units.
We are looking for medical engineers, software engineers and medical doctors willing to contribute and make the project useful for their part of the world as well as participating to the code-base and mechanical design.
We are planning on providing the control unit at a price of max 99$ or equivalent + shipping, while the whole kit would be provided for a low price aswell in case you're not willing to 3d print and manufacture all the components with your own resources, while keeping the project fully open source.
And yes of course we realize that the system is missing a mixing chamber for proper O2 concentration control as well as a humidifier. The humidifer and warmer is an external module on some ventilators, and maybe this module can be aquired separately.
Before commenting and saying that it is incomplete please read our current development schedule on github."

"Hardware development

TODO Shield PCB
TODO air mixing chamber to be added and O2 sensors to measure and control input oxygen concentration.
TODO air warmer and humidifier.
TODO a proper back-pressure PEEP valve controllable by other servos on the output.
TODO air filter on the output
TODO battery backup
Software development

TODO plot flow
TODO finalize PRVC mode
TODO detect servo failures and create a certain alarm sound. Adapt strength to compensate
TODO display measured values on the upper side of the LCD display
TODO create an OpenGL display on the computer that can display parameters and plot graphs at high resolutions
Online Resources

https://www.github.com/stm32openv
https://www.twich.tv/stm32openv
https://www.youtube.com/watch?v=p47dtsTrSBI
Support us: bc1qvfh9pt93a0lfqc73k87yw9q587tgxdwmyswrv8
"



-- 
## Jonathan Cline
## jcline@ieee.org
## Mobile: +1-805-617-0223
########################


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Re: [DIYbio] Re: Agrobacterium strains

I pinged u just now at the email address U shared. Cheers!

On Sun, Apr 25, 2021 at 11:27 PM Josh McGinnis <josh@everymanbio.com> wrote:
Hi Tom,

I'm in Los Angeles and among other things, I'm an independent researcher working to define a diy-friendly protocol for agro transformation of filamentous fungi. Most of my research is published on Instagram (@everymanbio) and related website. AGL1 and LBA4404 have both been used in transforming Agaricus bisporus (the white button mushroom) in the research that I'm replicating. 

My understanding is that this organism requires a permit, which I do not mind at all pursing. I'm just not sure exactly how to go about it. Perhaps that's something you or someone else in the community can help me make happen. 

I have a business / commercial postage address. Please feel free to email me directly if you'd like to follow-up in more detail: josh @ everymanbio.com.

Best,

- Josh

On Sunday, April 25, 2021 at 2:01:41 PM UTC-7 Tom De Medts wrote:
Where are you located? And what do you need the strains for?
Are you sure you would not benefit from any other strains?
I have with me the following 4 Agro strains:
  1. AGL1
  2. EHA105
  3. GV3101::pMP90
  4. LBA4404

I received these strains from an eminent university researcher at Purdue, to a shipping address of  a faculty member who knows me.

I am not sure it will be as easy to send you strains from my DIY / garage address to your address.
But I do not mind trying via FEDEX, USPS is almost guaranteed to be NOT delivered....



On Fri, Apr 23, 2021 at 5:47 AM everymanbio.com <jos...@mcginn.is> wrote:
I too am looking for LBA4404 or AGL1 and would be happy to make a donation for the trouble.

On Thursday, April 8, 2021 at 6:21:03 PM UTC-7 Tom De Medts wrote:
Dear all,

Would any of have any of these 4 Agro strains:
AGL1
EHA105
GV3101::pMP90
LBA4404

If yes, then would you be able to mail me a stab culture of the strain you can share in a sterile screw cap vial, please?

Thanks in advance.

Cheers!
tom

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[DIYbio] This technology had been used as a weapon



Without a doubt, there are a lot of errors in this testimonial since it was translated by software.

In 1990, I receive some technology in my body when two murders were blamed on me. I did not know the victims.

It is a rather special technology, today everyone can imagine, without difficulty, that one can introduce in the body of a human being a sophisticated system of surveillance.

This system allowed me to exonerate myself about these people who were suppressed, personally, I had chosen justice, I went to see several lawyers, I did not know this system. Canada is not participating alone in this type of investigation; the international community, i mean several countries, is involved.

I was contacted during the winter of 2018 to inform me that blood was flowing in the region of my heart. I was then told that I had been the victim of an attack. This technology had been used as a weapon to make me disappear as the investigation began to pin down those responsible for the summer 1990 murders. Contact involved a Canadian laboratory, as well as a Japanese team. Reports or tests would have been falsified to make the international community believe in a natural death. This crime, of great cowardice since one cannot defend oneself, obviously occurred in the winter of 2004, Canada was headed by Paul Martin.

Another step, I was offered $ 30,000 dollars to compensate me. I immediately refused it. Later, I suggested, trying to think as Canadians think, to ban for life, from these famous laboratories, those responsible for the crime. This time, it was they who refused and they went, or had already gone, paid the famous sum in another country, to an unidentified person.

I am therefore not bound by any agreement, which allows me to reveal here, to everyone, this sad story.

Steve Brown

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Re: [DIYbio] Re: Agrobacterium strains

Hi Tom,

I'm in Los Angeles and among other things, I'm an independent researcher working to define a diy-friendly protocol for agro transformation of filamentous fungi. Most of my research is published on Instagram (@everymanbio) and related website. AGL1 and LBA4404 have both been used in transforming Agaricus bisporus (the white button mushroom) in the research that I'm replicating. 

My understanding is that this organism requires a permit, which I do not mind at all pursing. I'm just not sure exactly how to go about it. Perhaps that's something you or someone else in the community can help me make happen. 

I have a business / commercial postage address. Please feel free to email me directly if you'd like to follow-up in more detail: josh @ everymanbio.com.


Best,

- Josh

On Sunday, April 25, 2021 at 2:01:41 PM UTC-7 Tom De Medts wrote:
Where are you located? And what do you need the strains for?
Are you sure you would not benefit from any other strains?
I have with me the following 4 Agro strains:
  1. AGL1
  2. EHA105
  3. GV3101::pMP90
  4. LBA4404

I received these strains from an eminent university researcher at Purdue, to a shipping address of  a faculty member who knows me.

I am not sure it will be as easy to send you strains from my DIY / garage address to your address.
But I do not mind trying via FEDEX, USPS is almost guaranteed to be NOT delivered....



On Fri, Apr 23, 2021 at 5:47 AM everymanbio.com <jos...@mcginn.is> wrote:
I too am looking for LBA4404 or AGL1 and would be happy to make a donation for the trouble.

On Thursday, April 8, 2021 at 6:21:03 PM UTC-7 Tom De Medts wrote:
Dear all,

Would any of have any of these 4 Agro strains:
AGL1
EHA105
GV3101::pMP90
LBA4404

If yes, then would you be able to mail me a stab culture of the strain you can share in a sterile screw cap vial, please?

Thanks in advance.

Cheers!
tom

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Re: [DIYbio] Re: Agrobacterium strains

Where are you located? And what do you need the strains for?
Are you sure you would not benefit from any other strains?
I have with me the following 4 Agro strains:
  1. AGL1
  2. EHA105
  3. GV3101::pMP90
  4. LBA4404

I received these strains from an eminent university researcher at Purdue, to a shipping address of  a faculty member who knows me.

I am not sure it will be as easy to send you strains from my DIY / garage address to your address.
But I do not mind trying via FEDEX, USPS is almost guaranteed to be NOT delivered....



On Fri, Apr 23, 2021 at 5:47 AM everymanbio.com <joshua@mcginn.is> wrote:
I too am looking for LBA4404 or AGL1 and would be happy to make a donation for the trouble.

On Thursday, April 8, 2021 at 6:21:03 PM UTC-7 Tom De Medts wrote:
Dear all,

Would any of have any of these 4 Agro strains:
AGL1
EHA105
GV3101::pMP90
LBA4404

If yes, then would you be able to mail me a stab culture of the strain you can share in a sterile screw cap vial, please?

Thanks in advance.

Cheers!
tom

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Re[4]: [DIYbio] Substitute for calf serum?

So far my understanding is that for culturing stem cells the medium consists of  a base containing nutrients and antibiotics such as DMEM/F12. Then typically 20% FBS is added for the growth factors. This is the part that is now commonly substituted by more precise or human derived component. Platelet lysate seems to be the recommended substitute.  Since one of the goals of this experiment is to make it as cheap as possible, I would like to make my own platelet lysate  rather than buy it (https://www.stemcell.com/human-platelet-lysate.html). I've made PRP and PRF many times. What i don't know how to do is figure out how much to make and add to the base medium.

------ Original Message ------
From: "Bryan Jones" <bryanjjones@gmail.com>
Sent: 4/14/2021 11:44:07 AM
Subject: Re: Re[2]: [DIYbio] Substitute for calf serum?

Here's a pretty good summary article on different alternatives to FBS. https://pharma.lonza.com/-/media/Lonza/knowledge/CGT/A_consensus_introduction_to_serum_replacements_CGT.pdf
There is also a database of articles that have reported non-FBS growth media for various cell types: https://fcs-free.org/fcs-database
I think what S James Parsons is talking about is a defined media (where each protein, growth-factor, salt, and other component is explicitly defined) . There are companies that make defined media, but I'm not sure if any are as good or affordable as FBS-based media. It is very desirable to use something defined and animal-free for any GMP product or product to be used in the clinic.
--Bryan Jones


On Mon, Apr 12, 2021 at 1:20 PM Frank <fgarcia0007@gmail.com> wrote:
I did a little more reading https://www.frontiersin.org/articles/10.3389/fcell.2016.00115/full and it turns out that human serum works even better than calf serum. derivatives of this that are  serum, plasma, platelet rich plasma, platelet poor plasma, and platelet lysate. I can easily make these myself from my own blood for the price of blood collection tubes or $0 with zero cruelty compared to $1000 for a liter of FBS which is obtained in a hideously cruel way. This article and others ouitline the many advantages to abandoning calf serum.

Yay!


------ Original Message ------
From: "S James Parsons Jr" <sjamesparsonsjr@gmail.com>
Sent: 4/12/2021 10:09:31 AM
Subject: Re: [DIYbio] Substitute for calf serum?

Human serum would work, the problem is cost and amount of material required.  

My idea is to create a profile of FBS, salt, proteins, the the like. Then find the minimum of each protein, salt, and growth factor needed for tissue culturing.   Once identified, create a transmutated organism (yeast, bacteria) to produce said proteins.  Then use that elixir as a replacement for FBS.

Boom, free hamburgers for life! 




On Apr 12, 2021, at 12:33 AM, Frank <fgarcia0007@gmail.com> wrote:

Thanks James or responding to my question. Your answer made me curious as I find it hard to believe that there could only be one product on the market for anything .  I found what appears to be a bunch of products marketed as substitutes for calf serum with some interesting advantages. Here are a couple. What do you think of these? Are they calf serum alternatives

Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media.  C Rauch, E Feifel, EM Amann, HP Spötl… - ALTEX-Alternatives to …, 2011 - altex.org

 

------ Original Message ------
From: "S James Parsons Jr" <sjamesparsonsjr@gmail.com>
Sent: 4/11/2021 3:07:16 PM
Subject: Re: [DIYbio] Substitute for calf serum?

That is the holy grail of tissue culturing.  If you find a magic elixir with growth hormones, salts, proteins, enzymes, and a desired pH you would be a wealthy person! 

On Apr 11, 2021, at 2:52 PM, Frank <fgarcia0007@gmail.com> wrote:

Can anyone please tell me if I can use a substitute when a cell culturing protocol calls for calf serum? specifically, a substitute that does not involve cruelty to animals. is it possible to use my own blood serum?


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[DIYbio] Re: Agrobacterium strains

I too am looking for LBA4404 or AGL1 and would be happy to make a donation for the trouble.

On Thursday, April 8, 2021 at 6:21:03 PM UTC-7 Tom De Medts wrote:
Dear all,

Would any of have any of these 4 Agro strains:
AGL1
EHA105
GV3101::pMP90
LBA4404

If yes, then would you be able to mail me a stab culture of the strain you can share in a sterile screw cap vial, please?

Thanks in advance.

Cheers!
tom

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Re[2]: [DIYbio] used cell incubator

BWB closed up and is currently formulating a plan to reopen in the future (not immediate). Genspace told me they are not equipped to do my experiment. 

------ Original Message ------
From: "Nathan McCorkle" <nmz787@gmail.com>
Sent: 4/20/2021 11:20:15 PM
Subject: Re: [DIYbio] used cell incubator

I thought there were two community biolabs in Brooklyn (genspace, biotech without borders), and also a decent DIY BIO community.

On Wed, Apr 14, 2021, 1:23 PM Frank <fgarcia0007@gmail.com> wrote:
Hi everyone
A while back I requested help defining a protocol for a therapeutic adipose derived stem cell procedure that I would like to perform. Unfortunately I didn't get as much feedback as I had hoped so I had to go ahead and choose an approach myself. I am now working on the protocol . I think I know all the materials I need except of course a cell culture hood or a proper laboratory to work in given that,amazingly, in New York City there isn't a biospace available . So I'm going ahead with it anyway and I think the only significant expense will be one piece of equipment. I'm not using Calf serum, substituting autologous platelet lysate. The missing piece I think is a cell incubator . I know there are many for sale used. But I have no idea what type, size, etc.  
I would love it if someone can tell me the minimum features I need for that incubator . All the information about what I'm doing and the materials list alright here on this working document that I posted in Google Docs. 

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Re: [DIYbio] used cell incubator

I thought there were two community biolabs in Brooklyn (genspace, biotech without borders), and also a decent DIY BIO community.

On Wed, Apr 14, 2021, 1:23 PM Frank <fgarcia0007@gmail.com> wrote:
Hi everyone
A while back I requested help defining a protocol for a therapeutic adipose derived stem cell procedure that I would like to perform. Unfortunately I didn't get as much feedback as I had hoped so I had to go ahead and choose an approach myself. I am now working on the protocol . I think I know all the materials I need except of course a cell culture hood or a proper laboratory to work in given that,amazingly, in New York City there isn't a biospace available . So I'm going ahead with it anyway and I think the only significant expense will be one piece of equipment. I'm not using Calf serum, substituting autologous platelet lysate. The missing piece I think is a cell incubator . I know there are many for sale used. But I have no idea what type, size, etc.  
I would love it if someone can tell me the minimum features I need for that incubator . All the information about what I'm doing and the materials list alright here on this working document that I posted in Google Docs. 

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Re: [DIYbio] It only takes 10 min: Take part in the DIY Bio Community Survey 2021 and win $150 gift card!

I'm interested to know what changes people think have happened regarding "the changes individuals and the community has gone through in the past decade".

My biggest view, at least from the activity on this list, is that the hype has died off and activity reduced. However  I'm also on a facebook group or two, and see there's still lots of interest, but on Facebook in general (not specific to the bio groups) I feel people's attention span is much more limited and fleeting. 

The other major change I see is The Odin becoming a mainstay supplier, both of materials and knowledge/training.

What do others think?

On Thu, Apr 15, 2021, 4:44 PM Anna Verena Eireiner <verena.eireiner@gmail.com> wrote:
It only takes 10 minutes: DIY Bio Community Survey 2021


Take this short survey and tell us about your experience in DIY bio. You can win one of three gift cards to a merchant of your choice valued $150, $50 and $30!

We'd appreciate it if you could circulate the link via twitter, mailing lists etc. The more DIY biologists take part, the better!

Given the experiences and the changes individuals and the community has gone through in the past decade, I would greatly appreciate it if you would take part in this 2021 DIY Biology Community Survey. Since the last survey of the DIYBio community was done in 2013, the purpose of this study is to help address issues of sensationalism and negative portrayals of DIYbio by updating and building a more holistic and empirically-based understanding of the community.

The results will be online as soon as the survey closes but will also be presented at the Global DIY bio Summit 2021. The more DIY bio people take part, the better!
Everyone who identifies as a member or participant of the DIYBio community can take part. If you have any questions, please do let me know! 

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Re: [DIYbio] Blood clot risk due to Corona vaccine

Hi, in addition to our conversation I found a new study that shines some light on the question whether mainly the astrazeneca vaccine might cause these problems:
https://www.researchsquare.com/article/rs-440461/v1


Mike Petersen schrieb am Mittwoch, 7. April 2021 um 19:53:14 UTC+2:
Hi Matt,
thank you for that information. I also think that the adenovirus is mainly responsible for the unwanted effect, even though it's unclear how the exaxt pathological mechanism may look like.
Personally I have a much higher tendency towards the mRNA vaccines due to the reasons you've described, since their mechanism means much less interference with the cellular genetics.


matt.e...@gmail.com schrieb am Montag, 5. April 2021 um 00:37:18 UTC+2:
Mike,

This is definitely outside my area of expertise but I suspect the thrombosis is a result of some other protein associated with the AstraZeneca vaccine, which is an adenovirus edited with the SARS-CoV2 spike protein gene ( ChAdOx1 NCoV-19), while the Moderna and Pfizer-BioNtech vaccines contain a much simpler genetic construct containing only part of the mRNA transcribed from the SARS-CoV2 spike protein gene.

This article describes the other transcription activity, aside from the SARS-CoV2 spike protein, associated with the AstraZeneca vaccine:


From what I've read briefly, the rest of the ingredients in the AstraZeneca vaccine have been used in prior vaccines.  The adenovirus used is a new thing, so I suspect that is the culprit.

My take away is that mRNA vaccines will be the vaccines of the future if their immune boost lasts as well as prior vaccines.  People can make them really fast, run huge amounts of trials, and lab technicians will not handle live virus except for those doing the initial harvesting and sequencing.  I imagine it'll be a lot cheaper, too.

Matt


On Apr 4, 2021, at 4:53 PM, 'Mike Petersen' via DIYbio <diy...@googlegroups.com> wrote:


Thank you for your answer drllau,
I think we are just on the threshold of personalized medicine. I did a 23andme test many years ago and am thinking about doing full genome sequencing.
With the 23andme raw data alone, I have an overview of tens of thousands of SNPs. Maybe this will give me some knowledge about whether I might fall into a risk class regarding the occurrence of adverse vaccine reactions.
For the risk of getting a severe corona disease, there are already numerous studies that determine which genetic variants lead to a higher risk on the basis of molecular genetic data.
For example this one:


drllau schrieb am Sonntag, 4. April 2021 um 05:59:50 UTC+2:
I've heard there's 6-7K "rare" defects (or tendencies to disease) that collectively affect 1 in 17 westerners. It sucks but when the genetic lottery is against you there's not much you can do unless you're a billionaire boomer willing to have your lineage tested as part of any drug trial. There's  still a long long way to go before personalised medicine, organ on a chip and computational metabolics can become a phase IV trial to eliminate such risks.

On Saturday, 3 April 2021 at 08:11:57 UTC+8 matt.e...@gmail.com wrote:
Mike,

Perhaps this article might reveal some effort on this front, and the names of those involved.


Matt

On Apr 2, 2021, at 7:18 PM, 'Mike Petersen' via DIYbio <diy...@googlegroups.com> wrote:

Hello everyone, if you follow the current situation in Europe, you will notice what a mess it is with the Astrazenica vaccine. The cases of sinus vein thrombosis and fatal blood clots have caused it to be partially withdrawn and then reintroduced with restrictions.

There is great debate about not overstating the risk, but of course it is tragic for any individual to die from a fatal vaccine side effect.
Wouldn't it be much more scientific to investigate what factors came together in those affected and led to these effects. What do I gain by referring to the low probability that it could occur in me and relying only on statistics. Wouldn't it be much safer and more scientific to determine the individual risk based on biomarkers (blood values, genetic features, etc.). The question is whether information is available, for example, studies that show commonalities of those affected. Are any of you aware of anything on this?
Thank you,
Mike

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[DIYbio] It only takes 10 min: Take part in the DIY Bio Community Survey 2021 and win $150 gift card!

It only takes 10 minutes: DIY Bio Community Survey 2021


Take this short survey and tell us about your experience in DIY bio. You can win one of three gift cards to a merchant of your choice valued $150, $50 and $30!

We'd appreciate it if you could circulate the link via twitter, mailing lists etc. The more DIY biologists take part, the better!

Given the experiences and the changes individuals and the community has gone through in the past decade, I would greatly appreciate it if you would take part in this 2021 DIY Biology Community Survey. Since the last survey of the DIYBio community was done in 2013, the purpose of this study is to help address issues of sensationalism and negative portrayals of DIYbio by updating and building a more holistic and empirically-based understanding of the community.

The results will be online as soon as the survey closes but will also be presented at the Global DIY bio Summit 2021. The more DIY bio people take part, the better!
Everyone who identifies as a member or participant of the DIYBio community can take part. If you have any questions, please do let me know! 

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Re: Re[4]: [DIYbio] Substitute for calf serum?

You're gonna give yourself cancer if you keep messing around with your own tissue, man.

On Wed, Apr 14, 2021, 2:24 PM Frank <fgarcia0007@gmail.com> wrote:
Thanks Bryan,
I had just come across that paper. Very useful. Seems like the thing to use is platelet lysate from my own blood.

------ Original Message ------
From: "Bryan Jones" <bryanjjones@gmail.com>
Sent: 4/14/2021 11:44:07 AM
Subject: Re: Re[2]: [DIYbio] Substitute for calf serum?

Here's a pretty good summary article on different alternatives to FBS. https://pharma.lonza.com/-/media/Lonza/knowledge/CGT/A_consensus_introduction_to_serum_replacements_CGT.pdf
There is also a database of articles that have reported non-FBS growth media for various cell types: https://fcs-free.org/fcs-database
I think what S James Parsons is talking about is a defined media (where each protein, growth-factor, salt, and other component is explicitly defined) . There are companies that make defined media, but I'm not sure if any are as good or affordable as FBS-based media. It is very desirable to use something defined and animal-free for any GMP product or product to be used in the clinic.
--Bryan Jones


On Mon, Apr 12, 2021 at 1:20 PM Frank <fgarcia0007@gmail.com> wrote:
I did a little more reading https://www.frontiersin.org/articles/10.3389/fcell.2016.00115/full and it turns out that human serum works even better than calf serum. derivatives of this that are  serum, plasma, platelet rich plasma, platelet poor plasma, and platelet lysate. I can easily make these myself from my own blood for the price of blood collection tubes or $0 with zero cruelty compared to $1000 for a liter of FBS which is obtained in a hideously cruel way. This article and others ouitline the many advantages to abandoning calf serum.

Yay!


------ Original Message ------
From: "S James Parsons Jr" <sjamesparsonsjr@gmail.com>
Sent: 4/12/2021 10:09:31 AM
Subject: Re: [DIYbio] Substitute for calf serum?

Human serum would work, the problem is cost and amount of material required.  

My idea is to create a profile of FBS, salt, proteins, the the like. Then find the minimum of each protein, salt, and growth factor needed for tissue culturing.   Once identified, create a transmutated organism (yeast, bacteria) to produce said proteins.  Then use that elixir as a replacement for FBS.

Boom, free hamburgers for life! 




On Apr 12, 2021, at 12:33 AM, Frank <fgarcia0007@gmail.com> wrote:

Thanks James or responding to my question. Your answer made me curious as I find it hard to believe that there could only be one product on the market for anything .  I found what appears to be a bunch of products marketed as substitutes for calf serum with some interesting advantages. Here are a couple. What do you think of these? Are they calf serum alternatives

Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media.  C Rauch, E Feifel, EM Amann, HP Spötl… - ALTEX-Alternatives to …, 2011 - altex.org

 

------ Original Message ------
From: "S James Parsons Jr" <sjamesparsonsjr@gmail.com>
Sent: 4/11/2021 3:07:16 PM
Subject: Re: [DIYbio] Substitute for calf serum?

That is the holy grail of tissue culturing.  If you find a magic elixir with growth hormones, salts, proteins, enzymes, and a desired pH you would be a wealthy person! 

On Apr 11, 2021, at 2:52 PM, Frank <fgarcia0007@gmail.com> wrote:

Can anyone please tell me if I can use a substitute when a cell culturing protocol calls for calf serum? specifically, a substitute that does not involve cruelty to animals. is it possible to use my own blood serum?


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