Fwd: Urgent Client Need: Software Engineer; Newton, MA; 12+ Months Contract possible of Extension. (Locals Only)

Please send your resumes at  niranjan.kumar@vision3solutions.com

 

Hi

 

This is Niranjan from Vision3solutions; hope you are doing well.

Please go through below description and reply with your resume, contact details and current location, if you feel comfortable

 

Title: Software Engineer

Location: Newton, MA

Duration: 12+ Months Contract possible of Extension.

 

Interview: Telephonic And Face to Face

 

Locals Only

 

Candidates that Must have experience with Java/AWS/Cassandra.

MUST HAVES

·         6+ years of experience as a Java Developer

·         Java/AWS/Cassandra

·         2+ Experience working with REST & SOAP

·         Excellent communication skills.

 

 

Thanks& Regards

 

Niranjan Kumar

 

cid:image001.jpg@01D0705F.83E60BC0

 

Vision3 Solutions Inc

8155 Cavendish Place,Suwanee, GA 30024
Contact No:
(678) 554-4785
Email: niranjan.kumar@vision3solutions.com

                 niruk5503@gmail.com


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Urgent Client Need: Software Engineer; Newton, MA; 12+ Months Contract possible of Extension. (Locals Only)

Please send your resumes at  niranjan.kumar@vision3solutions.com

 

Hi

 

This is Niranjan from Vision3solutions; hope you are doing well.

Please go through below description and reply with your resume, contact details and current location, if you feel comfortable

 

Title: Software Engineer

Location: Newton, MA

Duration: 12+ Months Contract possible of Extension.

 

Interview: Telephonic And Face to Face

 

Locals Only

 

Candidates that Must have experience with Java/AWS/Cassandra.

MUST HAVES

·         6+ years of experience as a Java Developer

·         Java/AWS/Cassandra

·         2+ Experience working with REST & SOAP

·         Excellent communication skills.

 

 

Thanks& Regards

 

Niranjan Kumar

 

cid:image001.jpg@01D0705F.83E60BC0

 

Vision3 Solutions Inc

8155 Cavendish Place,Suwanee, GA 30024
Contact No:
(678) 554-4785
Email: niranjan.kumar@vision3solutions.com

                 niruk5503@gmail.com

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Re: [DIYbio] E. coli with pVIB not glowing, why?


On Feb 25, 2017 9:56 AM, "MC" <markyching@gmail.com> wrote:
>
> That's really interesting, I'm going to have to do some reading on recombination. Both my cultures were DH10B.

Oh, ok, I guess the-odin changed how the sell pVIB... their product page showed this week that they ship pVIB in BL21.

Hard to say what could've happened from my end, then.

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Re: [DIYbio] E. coli with pVIB not glowing, why?

I transformed it at university 4-5 years ago. Replated it on LB without Amp lots of times and it got less bright (plasmid loss), but even after 6 subcultures it was still possible to see with the naked eye. Then I left the Amp culture in the Uni fridge over the summer holidays, and after like 3 month put it on fresh LB Amp - glowed again.

So from my experience pVIB worked fine. I don't know which exact coli strain we had at Uni, they were commercial ones

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Re: [DIYbio] Re: Preferred cheap countertop surface?

On Saturday, February 25, 2017 at 1:47:51 AM UTC-8, cory....@gmail.com wrote:

For super durable workbenches I start with a single coat of an oil-based wood primer like Kilz, then two coats of black spray-on appliance epoxy.  This stuff is really really nasty (the solvent is a mixture of acetone, petroleum, xylene, and butyl acetate). You'll want to do it outside and with a respirator + organic vapor filter, goggles, gloves, disposable clothes etc.  If you do it inside you'll end up with a black residue on everything that will never come off.  But this surface is incredibly durable.  It doesn't scratch, ever.  You can hit it with a hammer and it won't leave any sort of impression.  I cut stuff with razor blades on it frequently and it doesn't leave any marks. Resistant to direct flames for a short period of time.  Also, its resistant to acids and bases.  I've tested it with glacial acetic, sulfuric, 12M HCl, 10M NaOH, and 10M KOH.  The only things I've found that will eat through it are Piranha solution and cesium hydroxide solutions (don't try this without a fume hood and chem lab training/experience).

Ooh - that seems like a great find! I know we did a lot of soul searching for how to outfit CCL way back when as well. We wound up getting lucky with a donation of three very large lab benches, but we were initially looking for granite countertops, glass tables, stainless tables, etc - pretty much the story of this thread. If we had known about appliance Epoxy, I think we would likely have gone with that.

The original lab benches at BioCurious were DIY ones made from construction pipe with a top of this paneling with a whiteboard coating. Which was great in the beginning, because you could just write right on the bench with a dry-erase marker. But the whiteboard material wasn't terribly scratch resistant, so over time the benches developed scratches and holes through the top whiteboard layer, allowing liquids to seep in the fiberboard underneath - Not Good!

Patrik

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[DIYbio] Salicylate generator part

hi so i was wondering if we add this Part:BBa_J45320 to a e coli with a good promoter
could it yield enought methyl salicylate to be worthy instead of synthesising it.

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[DIYbio] chorismate to Salicylate

Hi
i was looking at this Part:BBa_J45320  
i was wondering this is a good odor signal but will it produce enought salicylate  to be useful in something else
like instead of synthetising methyl salicylate would not it better to just do a liquid culture of e coli that generate methyl salicylate
what kind of concentration could we expect in a liquid culture

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Re: [DIYbio] E. coli with pVIB not glowing, why?

That's really interesting, I'm going to have to do some reading on recombination. Both my cultures were DH10B. I have some BL21 I'm checking if are still alive from the fridge. It'll be fun to transform BL21 and see if it's any different. Thanks for the insight!

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Re: [DIYbio] Callus with Plant Tissue Culture

If this isn't a callus, what could it be?

Hmm, not sure.  All the callus I have experience with has been white or light green.  I'm not sure why it looks so red.  Honestly, if I didn't know I was looking for callus I would have guessed that was seed coat.

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Re: [DIYbio] Re: Preferred cheap countertop surface?

I'm a big fan of plywood workbench tops.  I usually use 3/4 inch thick plywood with a smooth surface (the cheap stuff will look terrible and you'll wish you'd spent the extra $20).  Sometimes it's called "cabinet grade" plywood.  I cover the edges with iron-on wood veneer tape so the sides are smooth.

For an ultra smooth mirror finish I put 2 coats of Varathane Ebony which makes the wood jet black. Then two coats of gloss polyurethane.  This looks really sharp and is fairly durable for general lab use.  But it *will* scratch easily with sharp objects.  I'm not sure how resistant it is to acids and bases.

For super durable workbenches I start with a single coat of an oil-based wood primer like Kilz, then two coats of black spray-on appliance epoxy.  This stuff is really really nasty (the solvent is a mixture of acetone, petroleum, xylene, and butyl acetate). You'll want to do it outside and with a respirator + organic vapor filter, goggles, gloves, disposable clothes etc.  If you do it inside you'll end up with a black residue on everything that will never come off.  But this surface is incredibly durable.  It doesn't scratch, ever.  You can hit it with a hammer and it won't leave any sort of impression.  I cut stuff with razor blades on it frequently and it doesn't leave any marks. Resistant to direct flames for a short period of time.  Also, its resistant to acids and bases.  I've tested it with glacial acetic, sulfuric, 12M HCl, 10M NaOH, and 10M KOH.  The only things I've found that will eat through it are Piranha solution and cesium hydroxide solutions (don't try this without a fume hood and chem lab training/experience).

Not quite as durable as a phenolic surface but for my purposes it's sufficient.  Plus it far less dense than phenolic so you can carry it yourself.


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[DIYbio] Re: Preferred cheap countertop surface?

For a completely off-the-wall idea, copper surfaces are strongly antibacterial. You can get a 2" x 4" sheet of 24ga copper for less than $150. Get yourself a free second hand table and some copper rivets and go to town.

Not terribly acid resistant obviously, but very bio-safe, and would make for a great steampunk lab. :-)

Patrik

PS: Make sure to accessorize with some cheapass DIY pipette holders made from copper pipe...

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Re: [DIYbio] Re: Preferred cheap countertop surface?

Thanks everyone!

Glass was recommended by another friend locally, and he also mentioned
looking for demolition sites.

Stainless isn't something I considered but momentarily... it indeed
would be a good choice, and I know I've seen stainless
commercial-kitchen tables with a big shelf underneath, for storage or
e.g. a microwave.


I found a piece of granite today for $5 per sq ft... there was an ad
on craigslist for free scrap pieces, I searched their free pile and
came up empty, so sent them an email asking if they had any larger
remnants or scrap, and voila!
http://imgur.com/lLjgREq

I still have to add some cross-bracing on the legs, and a shelf
underneath (I haven't decided if I want to try adding doors to the
lower area... but maybe some sliding plexiglass to keep dust out)

Thanks all!

On Fri, Feb 24, 2017 at 11:47 PM, Patrik D'haeseleer <patrikd@gmail.com> wrote:
> I'm seeing a good number of glass tabletops and stainless steel tables on
> Craigslist. With some patience, you can probably find a 2" x 4" stainless
> table for less than $100. That's not dirt cheap, but a worthwhile investment
> for something you'll be using daily for the next couple of years. Of course,
> the main drawback of stainless is that you can't use bleach on it, or it
> will rust.
>
> We actually bought a glass top table off Craigslist for CCL, just so we can
> tape some LED strip lights to it and take some CSI style lab pictures ;-)
>
> Patrik
>
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[DIYbio] Re: Preferred cheap countertop surface?

I'm seeing a good number of glass tabletops and stainless steel tables on Craigslist. With some patience, you can probably find a 2" x 4" stainless table for less than $100. That's not dirt cheap, but a worthwhile investment for something you'll be using daily for the next couple of years. Of course, the main drawback of stainless is that you can't use bleach on it, or it will rust.

We actually bought a glass top table off Craigslist for CCL, just so we can tape some LED strip lights to it and take some CSI style lab pictures ;-)

Patrik

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Re: [DIYbio] E. coli with pVIB not glowing, why?

When I was reading about the strain, a lot of folks complained about
recombination being possible (at least for long term maintenance of
the plasmid, or for any kind of sequence-sensitive work, like storing
a DNA library prior to sequencing)... I was actually thinking of
getting that strain and trying to select for reversions back to the
parent plasmid, using tetracycline...
https://en.wikipedia.org/wiki/PBR322

If you still have some culture around, you might try picking up some
tetracycline from a pet store and making amp+tet plates and seeing if
anything will grow.

Hard to say what happened, as I'm not too familiar with the
bioluminescent pathway and conditions for optimal light production...
but that is what comes to mind from my recent reading on pVIB and
BL21... waiting on an order myself :)

On Fri, Feb 24, 2017 at 11:24 PM, MC <markyching@gmail.com> wrote:
>> Did you grow with ampicillin?
>
>
> Yup, it came with amp media. Couldn't have been easier.
>
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Re: [DIYbio] E. coli with pVIB not glowing, why?

Did you grow with ampicillin?

Yup, it came with amp media. Couldn't have been easier. 

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Re: [DIYbio] E. coli with pVIB not glowing, why?

Did you grow with ampicillin?

On Fri, Feb 24, 2017 at 11:01 PM, MC <markyching@gmail.com> wrote:
> My failed pVIB E. coli experiment has been bugging me and I'm wondering if
> anyone has any ideas as to why. I got the pre-transformed kit from The Odin
> which was simple enough. My initial kit did not glow out of the box so
> Josiah was kind enough to send another one which I verified was glowing. I
> made additional LB plates and streaked them using the original glowing
> plate.
>
> Easy enough right? Except none of my plates glowed. Zero. I tried different
> temps, giving them more time to grow, etc. There was also no visible
> contamination and the cultures appeared to be growing fine. What could be
> the cause of the lack of glow?
>
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[DIYbio] E. coli with pVIB not glowing, why?

My failed pVIB E. coli experiment has been bugging me and I'm wondering if anyone has any ideas as to why. I got the pre-transformed kit from The Odin which was simple enough. My initial kit did not glow out of the box so Josiah was kind enough to send another one which I verified was glowing. I made additional LB plates and streaked them using the original glowing plate.

Easy enough right? Except none of my plates glowed. Zero. I tried different temps, giving them more time to grow, etc. There was also no visible contamination and the cultures appeared to be growing fine. What could be the cause of the lack of glow?

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Re: [DIYbio] Preferred cheap countertop surface?

Keep an eye out for school demolition, I got a truckload of those epoxy counters, plumbing, and sinks from a chem classroom a few years ago. They're all installed at the local hackspace.

Personally, I've got an ikea workbench with a few foot wide sheets of tempered glass laid across it. When they get really messy, I just run them in the dishwasher.

On Thu, Feb 23, 2017 at 8:08 PM, John Griessen <john@industromatic.com> wrote:
On 02/23/2017 04:27 PM, Nathan McCorkle wrote:
I'm going to scan craigslist again for some cheap granite too.

That and keep an eye open for construction cast offs.
A $20 cheap harbor freight 4 inch grinder plus a $7 diamond blade will
cut through granite when you spray water on it as you go.
Also the top edges of the cuts can be beveled that way.

Also consider remodeling trash that is "cultured marble" counter tops.
My real lab benches are made of black "cultured marble" that is dense,
fire resistant, acid proof, strong, smooth, cleanable, polishes well.
They are made of some kind of 2 part resin and rock dust in Taylor Texas.

Too bad you can't ship a 130 lb lab bench top with backsplash -- I have extra.

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Urgent need of SAP PO Consultant /Sunnyvale CA

Hi Greetings,

 

I have an Urgent requirement SAP PO Consultant with one of our direct client, in location Sunnyvale CA If you are interested in this requirement, please revert me back with updated resume.

 

Role: SAP PO Consultant

Location: Sunnyvale CA

Type of JOB:  contract (6 MONTHS) Extendable

(Onsite Role)

 

 

Rate:52/hr on C2C MAX

Responsibilities:

 

·         In depth knowledge of supporting SAP PI/PO environment 

·         At least 3 years of experience in implementation of integration solution using SAP PO (PI, BRM and NW BPM) 

·         Experience in Design and Configuration of integration scenarios and flows 

·         Should be able to troubleshoot the performance issues in a SAP PO implementation 

·         Experience in Odata and Java

Thanks & Regards

Sapna Dixit

Pyramid Consulting, Inc.

Executive Resourcing


Direct no: 415-943-9385 Sapna.dixit@pyramidci.com  Web: www.pyramidci.com

downloadhttps://www.linkedin.com/in/sapna-dixit-691b99b5

cid:image009.jpg@01D02C05.4FFC5560

 

WE FIND HIDDEN TALENT

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Re: [DIYbio] Preferred cheap countertop surface?

On 02/23/2017 04:27 PM, Nathan McCorkle wrote:
> I'm going to scan craigslist again for some cheap granite too.

That and keep an eye open for construction cast offs.
A $20 cheap harbor freight 4 inch grinder plus a $7 diamond blade will
cut through granite when you spray water on it as you go.
Also the top edges of the cuts can be beveled that way.

Also consider remodeling trash that is "cultured marble" counter tops.
My real lab benches are made of black "cultured marble" that is dense,
fire resistant, acid proof, strong, smooth, cleanable, polishes well.
They are made of some kind of 2 part resin and rock dust in Taylor Texas.

Too bad you can't ship a 130 lb lab bench top with backsplash -- I have extra.

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[DIYbio] Re: Preferred cheap countertop surface?

I use a plastic table with a large piece of plexiglass taped on top of it. The plexiglass is easy to clean and I can write on it with sharpies (and then erase with ethanol I use for sterilization). It works for me, and is pretty cheap, but I would much rather have a nice granite workbench. 

-Koeng

On Thursday, February 23, 2017 at 2:28:08 PM UTC-8, Nathan McCorkle wrote:
I am building a small workbench to compliment my second-kitchen
countertop that is a bit smaller than I need.

I tried finding some granite or other polished rock surfaces, but they
were asking $20-$50 per square foot. I'm wondering if a finish-grade
plywood with floor polyurethane would be OK for a few months/years of
casual use.

Obviously it sucks for fire prevention (if a bunsen burner fell over,
or an alcohol lamp), as well it seems less resistant to solvents and
strong chemicals, not that I'd care if it got ugly... but it might
change the hardness and be i.e. forever sticky and messy. My main
concern is microabrasions and being harder to sterilize. I think
flooring polyurethane will probably hold up pretty well to most
equipment and labware, except maybe my metal ring-stand. I'd guess I
might see some (micro) scratching even after dragging a dusty
cardboard box across the surface, due to the rocky components of dust
(at least my garage's dust). I'd bet bleach/alcohol/lysol would be
able to combat this for only so long.

I've also considered a few pieces of large/very-large non-porous
tile... but the grout joints concern me, though I imagine I might be
able to seal them somehow and they'd be much more fire-proof, but
still would be prone to scratching since the sealant would probably be
polyurethane or epoxy.

Am I overthinking it? What have other people found
works/doesn't-work/works-for-a-while?

I'm going to scan craigslist again for some cheap granite too.

--
-Nathan

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[DIYbio] Preferred cheap countertop surface?

I am building a small workbench to compliment my second-kitchen
countertop that is a bit smaller than I need.

I tried finding some granite or other polished rock surfaces, but they
were asking $20-$50 per square foot. I'm wondering if a finish-grade
plywood with floor polyurethane would be OK for a few months/years of
casual use.

Obviously it sucks for fire prevention (if a bunsen burner fell over,
or an alcohol lamp), as well it seems less resistant to solvents and
strong chemicals, not that I'd care if it got ugly... but it might
change the hardness and be i.e. forever sticky and messy. My main
concern is microabrasions and being harder to sterilize. I think
flooring polyurethane will probably hold up pretty well to most
equipment and labware, except maybe my metal ring-stand. I'd guess I
might see some (micro) scratching even after dragging a dusty
cardboard box across the surface, due to the rocky components of dust
(at least my garage's dust). I'd bet bleach/alcohol/lysol would be
able to combat this for only so long.

I've also considered a few pieces of large/very-large non-porous
tile... but the grout joints concern me, though I imagine I might be
able to seal them somehow and they'd be much more fire-proof, but
still would be prone to scratching since the sealant would probably be
polyurethane or epoxy.

Am I overthinking it? What have other people found
works/doesn't-work/works-for-a-while?

I'm going to scan craigslist again for some cheap granite too.

--
-Nathan

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Re: [DIYbio] Callus with Plant Tissue Culture

Just to confirm I'm identifying things correctly, this is a Drosera aliciae seedling with what I believe is a callus. I had sprouted these as well as a few other common species on a variety of media with different combinations of BAP and NAA for experimentation. If this isn't a callus, what could it be?

My plan is to let it grow out a little more before chopping it up to experiment with induction media.


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Urgent Client Need: Software Engineer; Newton, MA; 12+ Months Contract possible of Extension. (Locals Only)

Please send your resumes at  niranjan.kumar@vision3solutions.com

 

Hi

 

This is Niranjan from Vision3solutions; hope you are doing well.

Please go through below description and reply with your resume, contact details and current location, if you feel comfortable

 

Title: Software Engineer

Location: Newton, MA

Duration: 12+ Months Contract possible of Extension.

 

Interview: Telephonic And Face to Face

 

Locals Only

 

Candidates that Must have experience with Java/AWS/Cassandra.

MUST HAVES

·         6+ years of experience as a Java Developer

·         Java/AWS/Cassandra

·         2+ Experience working with REST & SOAP

·         Excellent communication skills.

Thanks& Regards

 

Niranjan Kumar

 

 

Vision3 Solutions Inc

8155 Cavendish Place,Suwanee, GA 30024
Contact No:
(678) 554-4785
Email: niranjan.kumar@vision3solutions.com

                 niruk5503@gmail.com


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Urgent Client Need: Software Engineer; Newton, MA; 12+ Months Contract possible of Extension. (Locals Only)

Please send your resumes at  niranjan.kumar@vision3solutions.com

 

Hi

 

This is Niranjan from Vision3solutions; hope you are doing well.

Please go through below description and reply with your resume, contact details and current location, if you feel comfortable

 

Title: Software Engineer

Location: Newton, MA

Duration: 12+ Months Contract possible of Extension.

 

Interview: Telephonic And Face to Face

 

Locals Only

 

Candidates that Must have experience with Java/AWS/Cassandra.

MUST HAVES

·         6+ years of experience as a Java Developer

·         Java/AWS/Cassandra

·         2+ Experience working with REST & SOAP

·         Excellent communication skills.

Thanks& Regards

 

Niranjan Kumar

 

 

Vision3 Solutions Inc

8155 Cavendish Place,Suwanee, GA 30024
Contact No:
(678) 554-4785
Email: niranjan.kumar@vision3solutions.com

                 niruk5503@gmail.com

 


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