[DIYbio] Re: If you want to start a community DIYbio lab in your city...

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среда, 27 марта 2019 г., 10:48:10 UTC+9 пользователь Seth Donnelley написал:
To anyone interesting in starting a community lab in your city and you don't already have one: I help DIY biologists to start a sustainable community lab in less than a month, and get started on the projects they dream of completing, by helping them gather a team of exceptional, like-minded individuals, building an unshakable organizational structure, and drafting a roadmap to financial sustainability. Let me know if you're interested. Thanks!  

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Re: [DIYbio] Re: Looking for partner or group for DIY TPE

As far as whether proprietary tests of epigenetic changes are the way to go I think that for measuring whether "rejuvenation" has occurred we don't need to spend a bunch of money or get too fancy. Just look in the mirror, so to speak.  If I am being rejuvenated then that's going to be reflected in my blood pressure, exercise capacity, sleep patterns, LDL, bone density, GFR, etc. So the standard diagnostic lab tests will reflect those changes.


On Tuesday, June 30, 2020 at 2:56:56 PM UTC-4 Frank Garcia wrote:
I guess we need to define rejuvenation then. the biomarker tests in the experiment each measures one tiny piece of the physiological state which, depending on the direction in which the marker changed, indicates a physiological state that more closely resembles that of a younger organism. I'm not sure (maybe i'm wrong) there is a universally accepted definition of rejuvenation. and there certainly isn't any single market that proves rejuvenation. It's simply too complex for that to ever be the case. It's more like pick your basket of markers of aging and test them over a period of time and see which way they are heading.  I personally think any markers of rejuvenation has to include those that measure the physiological dirvers of age -related disease and deterioration such as insulin sensitivity, fibrotic markers, resting heart rate, muscle stem cells, mitochondrial markers, cholesterol trajectory, cardiac output efficiency, C-reactive protein, just to name a few out of a million.  In the study, muscle anabolism vs catabolism is in fact a good aging biomarker

On Tuesday, June 30, 2020 at 2:20:11 AM UTC-4 Raph N wrote:
The problem with the biomarkers used in the experiment is that they can't prove rejuvenation.  For example we know exercise improve lots of biomarkers, yet it does not rejuvenate you, or you could exercise your way to immortality. 

As far as we know epigenetic clock is the best marker of biological age. 


On Tue, Jun 30, 2020, 01:50 Frank Garcia <fgarc...@gmail.com> wrote:
I don't know too much about the epigenetic tests however it might be useful for you to take a look at the biomarker tests that were used in the experiment that I posted which started this discussion. They did pretty rigorous testing of multiple systems and tissues to determine efficacy.

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Re: [DIYbio] Re: Looking for partner or group for DIY TPE

I guess we need to define rejuvenation then. the biomarker tests in the experiment each measures one tiny piece of the physiological state which, depending on the direction in which the marker changed, indicates a physiological state that more closely resembles that of a younger organism. I'm not sure (maybe i'm wrong) there is a universally accepted definition of rejuvenation. and there certainly isn't any single market that proves rejuvenation. It's simply too complex for that to ever be the case. It's more like pick your basket of markers of aging and test them over a period of time and see which way they are heading.  I personally think any markers of rejuvenation has to include those that measure the physiological dirvers of age -related disease and deterioration such as insulin sensitivity, fibrotic markers, resting heart rate, muscle stem cells, mitochondrial markers, cholesterol trajectory, cardiac output efficiency, C-reactive protein, just to name a few out of a million.  In the study, muscle anabolism vs catabolism is in fact a good aging biomarker

On Tuesday, June 30, 2020 at 2:20:11 AM UTC-4 Raph N wrote:
The problem with the biomarkers used in the experiment is that they can't prove rejuvenation.  For example we know exercise improve lots of biomarkers, yet it does not rejuvenate you, or you could exercise your way to immortality. 

As far as we know epigenetic clock is the best marker of biological age. 


On Tue, Jun 30, 2020, 01:50 Frank Garcia <fgarc...@gmail.com> wrote:
I don't know too much about the epigenetic tests however it might be useful for you to take a look at the biomarker tests that were used in the experiment that I posted which started this discussion. They did pretty rigorous testing of multiple systems and tissues to determine efficacy.

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Re: [DIYbio] Re: Looking for partner or group for DIY TPE

That's true enough about commercial test, though there's an easy way to check their validity: Don't give the company your age, and see how accurate the results are.

However I still believe that in principle epigenetic age are the best marker for age. That it is hard to test doesn't change the results. In fact, if you want to be accurate, you can contact Steve Horvath and get his help for the testing. That's what I'm working on for stem cells and exosomes with a few test patients.

Cheers

Tel/Telegram/Whatsapp: +447399 108 586
Skype: raphfinex


On Tue, 30 Jun 2020 at 17:41, James Clement <clementlawyer@gmail.com> wrote:
Proprietary tests that haven't been validated independently (peer-reviewed and duplicated in other labs) are nice tools for doctors to convince their patients that whatever they're doing is working, but aren't useful scientifically. The same is true of cheap telomere tests, or other purported proprietary biomarkers, IMHO.

Cheers,
James

On Monday, June 29, 2020 at 7:55:58 PM UTC-4, Gary Dale wrote:
I am in my 60's and am considering donating plasma or blood also but want to try and verify if there is any rejuv. I am looking into epigenetic age tests that are available to run BEFORE donating and then after some number of donations.
This is my first day researching it but this kit seems interesting and not too expensive.


On Monday, June 29, 2020 at 2:10:05 PM UTC-5, Frank wrote:
James,

Thanks for the response.  I am actually close to 60. Unless I find a plasmapheresis machine for free I will likely not attempt this myself mainly due to the impracticality of extracting the amount of plasma needed manually/incrementally. I actually have an educational background in medicine though I am not a practicing physician. The avenue that I thought might be useful is donating plasma which amounts to a TPE treatment though the volume taken is still inadequate. I had bad luck trying that yesterday as I was turned away for not having an AB blood type. After looking into it I was told this was a mistake and that I should be able to donate so I will try again next week. If you are looking for trial subjects by all means sign me up.

On Monday, June 29, 2020 at 2:04:44 PM UTC-4 James Clement wrote:
Betterhumans has been looking at running an IRB-approved clinical trial using TPE for several years (we have run a half-dozen different antiaging clinical trials since 2016 and intend to run about eight in 2021). I was part of a small group that approached Dr Dobri Kiprov in 2014 to arrange for a TPE clinical trial, but he wanted $25,000 per patient for a single treatment. We have since done a lot more research on recurring TPE therapy and believe we can do far more cheaply than this, but there are lots of potential risks and side effects if you try to do it too frequently. Since Betterhumans is opening its own antiaging clinical trial medical office in the near future with our own medical staff, we have been waiting until it is operational to pursue this particular clinical trial further. Of course the latest Conboy paper only strengthened our desire to test this in elderly subjects, when we are set up to proceed. We also have all of the analytical instruments in our laboratory to determine whether it works (including an Illumina iScan sequencer to run Infinium 800k beadchips, the only validated method for the Horvath clocks, with whom we're collaborating).

I don't know you or the others who have responded to this personally, but assuming you are not in your mid 60s or older, I would highly recommend that you do not attempt to DIY this yourself. If you're under 50 then you have plenty of time to wait for multiple clinical trials to prove the real-life efficacy and safety of TPE for antiaging purposes, and work the kinks out. We are finding there are downsides and risks to all of the antiaging therapies we have tested so far, and at least for the time being they should be reserved for those who simply can't wait the 10 years or more for them to be properly tested and optimized.

Best regards,
James 

On Saturday, June 27, 2020 at 1:42:46 AM UTC-4, Frank wrote:
Recent published experiments like the one below from UC Berkeley, demonstrate multi-system rejuvenation from an existing widely available procedure, therapeutic plasma exchange TPE.  This is quite exciting and the paper is well worth reviewing. I am interested in begin able to perform TPE on myself with someone's help. If you are interested please let me know.





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[DIYbio] Re: Looking for partner or group for DIY TPE

Proprietary tests that haven't been validated independently (peer-reviewed and duplicated in other labs) are nice tools for doctors to convince their patients that whatever they're doing is working, but aren't useful scientifically. The same is true of cheap telomere tests, or other purported proprietary biomarkers, IMHO.

Cheers,
James

On Monday, June 29, 2020 at 7:55:58 PM UTC-4, Gary Dale wrote:
I am in my 60's and am considering donating plasma or blood also but want to try and verify if there is any rejuv. I am looking into epigenetic age tests that are available to run BEFORE donating and then after some number of donations.
This is my first day researching it but this kit seems interesting and not too expensive.


On Monday, June 29, 2020 at 2:10:05 PM UTC-5, Frank wrote:
James,

Thanks for the response.  I am actually close to 60. Unless I find a plasmapheresis machine for free I will likely not attempt this myself mainly due to the impracticality of extracting the amount of plasma needed manually/incrementally. I actually have an educational background in medicine though I am not a practicing physician. The avenue that I thought might be useful is donating plasma which amounts to a TPE treatment though the volume taken is still inadequate. I had bad luck trying that yesterday as I was turned away for not having an AB blood type. After looking into it I was told this was a mistake and that I should be able to donate so I will try again next week. If you are looking for trial subjects by all means sign me up.

On Monday, June 29, 2020 at 2:04:44 PM UTC-4 James Clement wrote:
Betterhumans has been looking at running an IRB-approved clinical trial using TPE for several years (we have run a half-dozen different antiaging clinical trials since 2016 and intend to run about eight in 2021). I was part of a small group that approached Dr Dobri Kiprov in 2014 to arrange for a TPE clinical trial, but he wanted $25,000 per patient for a single treatment. We have since done a lot more research on recurring TPE therapy and believe we can do far more cheaply than this, but there are lots of potential risks and side effects if you try to do it too frequently. Since Betterhumans is opening its own antiaging clinical trial medical office in the near future with our own medical staff, we have been waiting until it is operational to pursue this particular clinical trial further. Of course the latest Conboy paper only strengthened our desire to test this in elderly subjects, when we are set up to proceed. We also have all of the analytical instruments in our laboratory to determine whether it works (including an Illumina iScan sequencer to run Infinium 800k beadchips, the only validated method for the Horvath clocks, with whom we're collaborating).

I don't know you or the others who have responded to this personally, but assuming you are not in your mid 60s or older, I would highly recommend that you do not attempt to DIY this yourself. If you're under 50 then you have plenty of time to wait for multiple clinical trials to prove the real-life efficacy and safety of TPE for antiaging purposes, and work the kinks out. We are finding there are downsides and risks to all of the antiaging therapies we have tested so far, and at least for the time being they should be reserved for those who simply can't wait the 10 years or more for them to be properly tested and optimized.

Best regards,
James 

On Saturday, June 27, 2020 at 1:42:46 AM UTC-4, Frank wrote:
Recent published experiments like the one below from UC Berkeley, demonstrate multi-system rejuvenation from an existing widely available procedure, therapeutic plasma exchange TPE.  This is quite exciting and the paper is well worth reviewing. I am interested in begin able to perform TPE on myself with someone's help. If you are interested please let me know.





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Re: [DIYbio] Re: Looking for partner or group for DIY TPE

What are we looking at? What's the image saying?


On Tue, 30 Jun 2020 at 10:58, boj Ko <paulkov69@gmail.com> wrote:




It's a long way to go, if anything! - Are you ready for it?
First you need to go through a synergistic detoxification for about 5-6 months, then go through the enrichment of a number of organs with new cells through stimulation systems and only then begin invasive measures to influence long lengths in complex ways. (Whiskey and almost all delicious food and not only will leave your life for 7 years) Otherwise, the results will stagnate)))) Are you ready for this))))

вт, 30 июн. 2020 г. в 09:20, Raphael Nicolle <raphbot@gmail.com>:
The problem with the biomarkers used in the experiment is that they can't prove rejuvenation.  For example we know exercise improve lots of biomarkers, yet it does not rejuvenate you, or you could exercise your way to immortality. 

As far as we know epigenetic clock is the best marker of biological age. 


On Tue, Jun 30, 2020, 01:50 Frank Garcia <fgarcia0007@gmail.com> wrote:
I don't know too much about the epigenetic tests however it might be useful for you to take a look at the biomarker tests that were used in the experiment that I posted which started this discussion. They did pretty rigorous testing of multiple systems and tissues to determine efficacy.

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Re: [DIYbio] Re: Looking for partner or group for DIY TPE





It's a long way to go, if anything! - Are you ready for it?
First you need to go through a synergistic detoxification for about 5-6 months, then go through the enrichment of a number of organs with new cells through stimulation systems and only then begin invasive measures to influence long lengths in complex ways. (Whiskey and almost all delicious food and not only will leave your life for 7 years) Otherwise, the results will stagnate)))) Are you ready for this))))

вт, 30 июн. 2020 г. в 09:20, Raphael Nicolle <raphbot@gmail.com>:
The problem with the biomarkers used in the experiment is that they can't prove rejuvenation.  For example we know exercise improve lots of biomarkers, yet it does not rejuvenate you, or you could exercise your way to immortality. 

As far as we know epigenetic clock is the best marker of biological age. 


On Tue, Jun 30, 2020, 01:50 Frank Garcia <fgarcia0007@gmail.com> wrote:
I don't know too much about the epigenetic tests however it might be useful for you to take a look at the biomarker tests that were used in the experiment that I posted which started this discussion. They did pretty rigorous testing of multiple systems and tissues to determine efficacy.

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Re: [DIYbio] Re: Looking for partner or group for DIY TPE

The problem with the biomarkers used in the experiment is that they can't prove rejuvenation.  For example we know exercise improve lots of biomarkers, yet it does not rejuvenate you, or you could exercise your way to immortality. 

As far as we know epigenetic clock is the best marker of biological age. 


On Tue, Jun 30, 2020, 01:50 Frank Garcia <fgarcia0007@gmail.com> wrote:
I don't know too much about the epigenetic tests however it might be useful for you to take a look at the biomarker tests that were used in the experiment that I posted which started this discussion. They did pretty rigorous testing of multiple systems and tissues to determine efficacy.

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[DIYbio] Re: Looking for partner or group for DIY TPE

I don't know too much about the epigenetic tests however it might be useful for you to take a look at the biomarker tests that were used in the experiment that I posted which started this discussion. They did pretty rigorous testing of multiple systems and tissues to determine efficacy.

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[DIYbio] Re: Looking for partner or group for DIY TPE

I am in my 60's and am considering donating plasma or blood also but want to try and verify if there is any rejuv. I am looking into epigenetic age tests that are available to run BEFORE donating and then after some number of donations.
This is my first day researching it but this kit seems interesting and not too expensive.
https://epiagingusa.com/product/epiaging-kit/


On Monday, June 29, 2020 at 2:10:05 PM UTC-5, Frank wrote:
James,

Thanks for the response.  I am actually close to 60. Unless I find a plasmapheresis machine for free I will likely not attempt this myself mainly due to the impracticality of extracting the amount of plasma needed manually/incrementally. I actually have an educational background in medicine though I am not a practicing physician. The avenue that I thought might be useful is donating plasma which amounts to a TPE treatment though the volume taken is still inadequate. I had bad luck trying that yesterday as I was turned away for not having an AB blood type. After looking into it I was told this was a mistake and that I should be able to donate so I will try again next week. If you are looking for trial subjects by all means sign me up.

On Monday, June 29, 2020 at 2:04:44 PM UTC-4 James Clement wrote:
Betterhumans has been looking at running an IRB-approved clinical trial using TPE for several years (we have run a half-dozen different antiaging clinical trials since 2016 and intend to run about eight in 2021). I was part of a small group that approached Dr Dobri Kiprov in 2014 to arrange for a TPE clinical trial, but he wanted $25,000 per patient for a single treatment. We have since done a lot more research on recurring TPE therapy and believe we can do far more cheaply than this, but there are lots of potential risks and side effects if you try to do it too frequently. Since Betterhumans is opening its own antiaging clinical trial medical office in the near future with our own medical staff, we have been waiting until it is operational to pursue this particular clinical trial further. Of course the latest Conboy paper only strengthened our desire to test this in elderly subjects, when we are set up to proceed. We also have all of the analytical instruments in our laboratory to determine whether it works (including an Illumina iScan sequencer to run Infinium 800k beadchips, the only validated method for the Horvath clocks, with whom we're collaborating).

I don't know you or the others who have responded to this personally, but assuming you are not in your mid 60s or older, I would highly recommend that you do not attempt to DIY this yourself. If you're under 50 then you have plenty of time to wait for multiple clinical trials to prove the real-life efficacy and safety of TPE for antiaging purposes, and work the kinks out. We are finding there are downsides and risks to all of the antiaging therapies we have tested so far, and at least for the time being they should be reserved for those who simply can't wait the 10 years or more for them to be properly tested and optimized.

Best regards,
James 

On Saturday, June 27, 2020 at 1:42:46 AM UTC-4, Frank wrote:
Recent published experiments like the one below from UC Berkeley, demonstrate multi-system rejuvenation from an existing widely available procedure, therapeutic plasma exchange TPE.  This is quite exciting and the paper is well worth reviewing. I am interested in begin able to perform TPE on myself with someone's help. If you are interested please let me know.





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[DIYbio] Re: Looking for partner or group for DIY TPE

James,

Thanks for the response.  I am actually close to 60. Unless I find a plasmapheresis machine for free I will likely not attempt this myself mainly due to the impracticality of extracting the amount of plasma needed manually/incrementally. I actually have an educational background in medicine though I am not a practicing physician. The avenue that I thought might be useful is donating plasma which amounts to a TPE treatment though the volume taken is still inadequate. I had bad luck trying that yesterday as I was turned away for not having an AB blood type. After looking into it I was told this was a mistake and that I should be able to donate so I will try again next week. If you are looking for trial subjects by all means sign me up.

On Monday, June 29, 2020 at 2:04:44 PM UTC-4 James Clement wrote:
Betterhumans has been looking at running an IRB-approved clinical trial using TPE for several years (we have run a half-dozen different antiaging clinical trials since 2016 and intend to run about eight in 2021). I was part of a small group that approached Dr Dobri Kiprov in 2014 to arrange for a TPE clinical trial, but he wanted $25,000 per patient for a single treatment. We have since done a lot more research on recurring TPE therapy and believe we can do far more cheaply than this, but there are lots of potential risks and side effects if you try to do it too frequently. Since Betterhumans is opening its own antiaging clinical trial medical office in the near future with our own medical staff, we have been waiting until it is operational to pursue this particular clinical trial further. Of course the latest Conboy paper only strengthened our desire to test this in elderly subjects, when we are set up to proceed. We also have all of the analytical instruments in our laboratory to determine whether it works (including an Illumina iScan sequencer to run Infinium 800k beadchips, the only validated method for the Horvath clocks, with whom we're collaborating).

I don't know you or the others who have responded to this personally, but assuming you are not in your mid 60s or older, I would highly recommend that you do not attempt to DIY this yourself. If you're under 50 then you have plenty of time to wait for multiple clinical trials to prove the real-life efficacy and safety of TPE for antiaging purposes, and work the kinks out. We are finding there are downsides and risks to all of the antiaging therapies we have tested so far, and at least for the time being they should be reserved for those who simply can't wait the 10 years or more for them to be properly tested and optimized.

Best regards,
James 

On Saturday, June 27, 2020 at 1:42:46 AM UTC-4, Frank wrote:
Recent published experiments like the one below from UC Berkeley, demonstrate multi-system rejuvenation from an existing widely available procedure, therapeutic plasma exchange TPE.  This is quite exciting and the paper is well worth reviewing. I am interested in begin able to perform TPE on myself with someone's help. If you are interested please let me know.





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[DIYbio] Re: Looking for partner or group for DIY TPE

Betterhumans has been looking at running an IRB-approved clinical trial using TPE for several years (we have run a half-dozen different antiaging clinical trials since 2016 and intend to run about eight in 2021). I was part of a small group that approached Dr Dobri Kiprov in 2014 to arrange for a TPE clinical trial, but he wanted $25,000 per patient for a single treatment. We have since done a lot more research on recurring TPE therapy and believe we can do far more cheaply than this, but there are lots of potential risks and side effects if you try to do it too frequently. Since Betterhumans is opening its own antiaging clinical trial medical office in the near future with our own medical staff, we have been waiting until it is operational to pursue this particular clinical trial further. Of course the latest Conboy paper only strengthened our desire to test this in elderly subjects, when we are set up to proceed. We also have all of the analytical instruments in our laboratory to determine whether it works (including an Illumina iScan sequencer to run Infinium 800k beadchips, the only validated method for the Horvath clocks, with whom we're collaborating).

I don't know you or the others who have responded to this personally, but assuming you are not in your mid 60s or older, I would highly recommend that you do not attempt to DIY this yourself. If you're under 50 then you have plenty of time to wait for multiple clinical trials to prove the real-life efficacy and safety of TPE for antiaging purposes, and work the kinks out. We are finding there are downsides and risks to all of the antiaging therapies we have tested so far, and at least for the time being they should be reserved for those who simply can't wait the 10 years or more for them to be properly tested and optimized.

Best regards,
James 

On Saturday, June 27, 2020 at 1:42:46 AM UTC-4, Frank wrote:
Recent published experiments like the one below from UC Berkeley, demonstrate multi-system rejuvenation from an existing widely available procedure, therapeutic plasma exchange TPE.  This is quite exciting and the paper is well worth reviewing. I am interested in begin able to perform TPE on myself with someone's help. If you are interested please let me know.





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Re: [DIYbio] Re: Looking for partner or group for DIY TPE

There may be a kind of threshold effect to get the expected benefits.

It may also be that there will not be that much rejuvenation going on, see for example this article: https://joshmitteldorf.scienceblog.com/2020/06/29/human-trials-of-plasma-exchange/

Cheers

On Mon, 29 Jun 2020 at 12:05, Frank <fgarcia0007@gmail.com> wrote:
oh yeah those numbers are for me based on a total blood volume of 5.73 liters

On Monday, June 29, 2020 at 6:23:03 AM UTC-4 Frank wrote:
So far here is what i have surmised. The amount of blood taken during a donation varies by donation type, your height and weight, and the donation center. Donations are not supposed to exceed 15% of total blood volume but can be twice that for plasma depending on the individual donor for plasma. when you donate whole blood, the 1 pint or about 1/2 liter taken includes all components. if 60% is plasma that's  262 ML 7.8% of your plasma. When you donate plasma, the pint taken is all plasma so that's 15% of your plasma becasue the other blood components are re-infused.  One reason more total volume can be taken with plasma vs whole blood is that with whole blood there is no volume replacement whereas with plasmapheresis the volume of plasma taken is replaced with saline. So depending on the blood collection center you can ask to donate up to a liter of plasma which is about 30% of your plasma. that starts to approach the promised land.
But wait there's more! Whole blood donations can't be more frequent than once every 4-6 weeks or even longer for some places. But you can donate plasma once every 7-10 days. So in 7 days with two donations you could get very close to the mice in the experiment. The only thing that sucks is getting so much anticoagulant  infused.


On Sunday, June 28, 2020 at 5:36:57 PM UTC-4 Gary Dale wrote:

So, how is the Conboy NBE or TPE any different than just donateing blood or just plasma? Seems to me it's exactly the same except the Conboy's have discovered a NEW Anti Aging benefit. That of flushing out years of accumulated garbage (maybe broken components that the liver/kidneys can't filter out?). You don't replace 50% of your plasma at once but donating 1 pint of blood with 8 weeks between donations should add up :-)


On Saturday, June 27, 2020 at 12:42:46 AM UTC-5, Frank wrote:
Recent published experiments like the one below from UC Berkeley, demonstrate multi-system rejuvenation from an existing widely available procedure, therapeutic plasma exchange TPE.  This is quite exciting and the paper is well worth reviewing. I am interested in begin able to perform TPE on myself with someone's help. If you are interested please let me know.





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[DIYbio] Re: Looking for partner or group for DIY TPE

oh yeah those numbers are for me based on a total blood volume of 5.73 liters

On Monday, June 29, 2020 at 6:23:03 AM UTC-4 Frank wrote:
So far here is what i have surmised. The amount of blood taken during a donation varies by donation type, your height and weight, and the donation center. Donations are not supposed to exceed 15% of total blood volume but can be twice that for plasma depending on the individual donor for plasma. when you donate whole blood, the 1 pint or about 1/2 liter taken includes all components. if 60% is plasma that's  262 ML 7.8% of your plasma. When you donate plasma, the pint taken is all plasma so that's 15% of your plasma becasue the other blood components are re-infused.  One reason more total volume can be taken with plasma vs whole blood is that with whole blood there is no volume replacement whereas with plasmapheresis the volume of plasma taken is replaced with saline. So depending on the blood collection center you can ask to donate up to a liter of plasma which is about 30% of your plasma. that starts to approach the promised land.
But wait there's more! Whole blood donations can't be more frequent than once every 4-6 weeks or even longer for some places. But you can donate plasma once every 7-10 days. So in 7 days with two donations you could get very close to the mice in the experiment. The only thing that sucks is getting so much anticoagulant  infused.


On Sunday, June 28, 2020 at 5:36:57 PM UTC-4 Gary Dale wrote:

So, how is the Conboy NBE or TPE any different than just donateing blood or just plasma? Seems to me it's exactly the same except the Conboy's have discovered a NEW Anti Aging benefit. That of flushing out years of accumulated garbage (maybe broken components that the liver/kidneys can't filter out?). You don't replace 50% of your plasma at once but donating 1 pint of blood with 8 weeks between donations should add up :-)


On Saturday, June 27, 2020 at 12:42:46 AM UTC-5, Frank wrote:
Recent published experiments like the one below from UC Berkeley, demonstrate multi-system rejuvenation from an existing widely available procedure, therapeutic plasma exchange TPE.  This is quite exciting and the paper is well worth reviewing. I am interested in begin able to perform TPE on myself with someone's help. If you are interested please let me know.





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[DIYbio] Re: Looking for partner or group for DIY TPE

So far here is what i have surmised. The amount of blood taken during a donation varies by donation type, your height and weight, and the donation center. Donations are not supposed to exceed 15% of total blood volume but can be twice that for plasma depending on the individual donor for plasma. when you donate whole blood, the 1 pint or about 1/2 liter taken includes all components. if 60% is plasma that's  262 ML 7.8% of your plasma. When you donate plasma, the pint taken is all plasma so that's 15% of your plasma becasue the other blood components are re-infused.  One reason more total volume can be taken with plasma vs whole blood is that with whole blood there is no volume replacement whereas with plasmapheresis the volume of plasma taken is replaced with saline. So depending on the blood collection center you can ask to donate up to a liter of plasma which is about 30% of your plasma. that starts to approach the promised land.

But wait there's more! Whole blood donations can't be more frequent than once every 4-6 weeks or even longer for some places. But you can donate plasma once every 7-10 days. So in 7 days with two donations you could get very close to the mice in the experiment. The only thing that sucks is getting so much anticoagulant  infused.


On Sunday, June 28, 2020 at 5:36:57 PM UTC-4 Gary Dale wrote:

So, how is the Conboy NBE or TPE any different than just donateing blood or just plasma? Seems to me it's exactly the same except the Conboy's have discovered a NEW Anti Aging benefit. That of flushing out years of accumulated garbage (maybe broken components that the liver/kidneys can't filter out?). You don't replace 50% of your plasma at once but donating 1 pint of blood with 8 weeks between donations should add up :-)


On Saturday, June 27, 2020 at 12:42:46 AM UTC-5, Frank wrote:
Recent published experiments like the one below from UC Berkeley, demonstrate multi-system rejuvenation from an existing widely available procedure, therapeutic plasma exchange TPE.  This is quite exciting and the paper is well worth reviewing. I am interested in begin able to perform TPE on myself with someone's help. If you are interested please let me know.





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[DIYbio] JOGL Live #1 with Quantified Flu

Whether COVID-19, the flu or the common cold: Can wearables warn us when we're getting sick?

This new event is one of the many ways that JOGL supports open research and innovation. During a one-hour live call, we choose a project on JOGL and bring it into the spotlight.

We will talk with Bastian Greshake Tzovaras, Mad Price Ball and Gary Wolf about the Quantified Flu project. Can physiological parameters tracked by our wearables predict when we're getting sick? By launching a collaborative citizen science project around this, the 'Quantified Flu' team hopes to harness the collective intelligence of all participants to quickly get a better understanding of our collective data!

The team is looking for data analysts, students or experts. This is a great opportunity to create new collaborations!
Register here: https://www.eventbrite.com/e/jogl-live-1-with-quantified-flu-tickets-111136586522

More about JOGL:

Just One Giant Lab (JOGL) is the first research and innovation laboratory operating as a distributed, open and massive mobilisation platform for collaborative task solving. JOGL helps humanity to sync onto fixing our most urgent and important problems using Open Science, Responsible Innovation and Continuous Learning. Join us! https://jogl.io/

Best, 

Marianna Limas

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[DIYbio] One year of Open Science with JOGL!

JOGL (Just One Giant Lab) is turning one year old!

JOGL (https://jogl.io/) is a platform where users can launch their projects and collaborate with others to solve problems using open science, responsible innovation, and continuous learning! We partner with academic labs, community labs, companies, startups, foundations, NGOs, and public services to create participatory research programs for understanding and solving health, environmental, social, and humanitarian issues.
Celebrate 1 year of open science on JOGL with us by tuning in on Wednesday July 8th at 4pm UTC/6 pm CET/12 pm EDT. 🎂
Register here: https://www.eventbrite.com/e/one-year-of-open-science-with-jogl-tickets-110758579894

  • Hear from project teams about how JOGL has helped them achieve their goals
  • Meet organisations who work with us on advancing openness and collaboration in science and innovation.
  • Mingle with the community and share your ideas on what the future of Open Science and JOGL should be!

Detailed agenda coming soon!

Best, 

Marianna Limas

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RE: [DIYbio] Re: If you want to start a community DIYbio lab in your city...

Yes! I need your help for this!

 

From: Alexandra P
Sent: Sunday, June 28, 2020 10:28 AM
To: DIYbio
Subject: [DIYbio] Re: If you want to start a community DIYbio lab in your city...

 

Hi Seth! How can I contact you?

среда, 27 марта 2019 г., 10:48:10 UTC+9 пользователь Seth Donnelley написал:

To anyone interesting in starting a community lab in your city and you don't already have one: I help DIY biologists to start a sustainable community lab in less than a month, and get started on the projects they dream of completing, by helping them gather a team of exceptional, like-minded individuals, building an unshakable organizational structure, and drafting a roadmap to financial sustainability. Let me know if you're interested. Thanks!  

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[DIYbio] Re: Looking for partner or group for DIY TPE


So, how is the Conboy NBE or TPE any different than just donateing blood or just plasma? Seems to me it's exactly the same except the Conboy's have discovered a NEW Anti Aging benefit. That of flushing out years of accumulated garbage (maybe broken components that the liver/kidneys can't filter out?). You don't replace 50% of your plasma at once but donating 1 pint of blood with 8 weeks between donations should add up :-)


On Saturday, June 27, 2020 at 12:42:46 AM UTC-5, Frank wrote:
Recent published experiments like the one below from UC Berkeley, demonstrate multi-system rejuvenation from an existing widely available procedure, therapeutic plasma exchange TPE.  This is quite exciting and the paper is well worth reviewing. I am interested in begin able to perform TPE on myself with someone's help. If you are interested please let me know.





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[DIYbio] Re: If you want to start a community DIYbio lab in your city...

Hi Seth! How can I contact you?

среда, 27 марта 2019 г., 10:48:10 UTC+9 пользователь Seth Donnelley написал:
To anyone interesting in starting a community lab in your city and you don't already have one: I help DIY biologists to start a sustainable community lab in less than a month, and get started on the projects they dream of completing, by helping them gather a team of exceptional, like-minded individuals, building an unshakable organizational structure, and drafting a roadmap to financial sustainability. Let me know if you're interested. Thanks!  

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Re: [DIYbio] Re: Establishing a New Haven Community Bio Space - Equipment List

Hi JR,

I completely agree in principle with all of Patrik's comments.

I have a few more things to add based on my experience working in two very contrasting environments - 
PhD and postdoc research in tier 1 universities, versus setting up and working in my garage lab.

Here are my personal experiences:
1. You can buy items off of E-bay at a 10-50% discount compared to brand new items
2. The equipment you will need will depend on the list of experiments you wish to perform (I cannot see that description in your email or Google file)
3. The list of experiments in turn will depend on the 'learning goals' of your community lab  (I cannot see that description in your email or Google file)
4. The 'learning goals' in turn will depend on the target audience's intellectual capacity (HS vs. college vs DIYers Vs. pros), 
time investment by stakeholders (weekend vs evenings),  and your business model vis-a-vis funding - self-sufficient vs. grant-based being approximately on the opposite ends of this spectrum.  (I cannot see such details in your email or Google file)
5. If you can reduce capital expense, but still retain the same pot of gold, you would have more for running expenses
- and depending on the type of experiments, these can be non-trivial. This point has been made by others in other posts here as well,
and it is an oft under-appreciated aspect of any research.
6. How much of your lab will be community-based, and how much will be DIY-based? Allow me to explain.
If you go hardcore into DIY, you can build you own laminar flow - I built mine for < $600, and it has an adjustable flow rate of > 100fpm and is more that sufficient for BSL-1. But this will take time for procuring the right items, for the right price. SO it's a trade-off and for such DIY sub-projects to not end up being distractions, it would be best to source experienced personnel to help out. Alternatively, if folks in your own community can build a DIY laminar flow (as in this example), then you would start off with a sense of community and participation already, and perhaps folks in your neighborhood would be already invested?
7. Finally, pardon me for being so blunt in my assessment, this is by NO MEANS an attack on you - in fact, I appreciate that you reached out to this community for advice [ From looking you up. I suspect you may not a(n extensive) background in science ] - which means you are open-minded, hence this point - An important aspect of community/DIY is innovation - if one cannot be imaginative in reducing up front costs and time required for design, build and procurement of equipment, budgeting for consumables etc., I think there'd be very little hope that such a venture would stay afloat. Frugality without compromising on the quality of science is IMHO a large part of innovation. A lot of well-intentioned community labs and DIY projects fail because they do not factor in all of the moving parts...

If you want to discuss specifics, I can connect you to a colleague in my garage lab who can better share all the gory details of equipment choice, procurement, design/build etc. 

But it would help if you can provide some of the missing context (2,3,4,6 above), so our suggestions may be better tailored.

Good luck!
Tom


On Sun, Jun 28, 2020 at 3:51 AM Patrik D'haeseleer <patrikd@gmail.com> wrote:
$20K is a real luxury, but please don't blow it all on buying brand new equipment if you can get functionally the same gear for as little as a tenth of the price second hand on eBay, CraigsList, or auction sites. 

- Definitely do yourself a favor and get a real biosafety cabinet instead of the laminar flow
- The incubator you have listed seems tiny. Get an undercounter one. You might need to store 50 or more plates if you want to run Agar Art workshops. Maybe get the tiny one as well, in case you need to incubate at two different temperatures - e.g. yeast vs E. coli
- No need for a plasmapheresis machine - you should not be working with blood in a BSL-1 lab. 
- That Formlabs printer is 1/3 of your entire budget - are you really, really sure you need an expensive high-end resin printer like this? As someone who helps run the bioprinter group at BioCurious, I wouldn't dream of getting this one, unless you knew *exactly* how you will be using it and why it needs to be this particular model, and you're planning for this to be a major feature of what you do at your community lab. If you just want a resin printer, there are several good ones under $1000 now.
- A large centrifuge like that can be quite dangerous. Also not really that useful unless you start growing large volumes of cells, but then you should invest in bioreactors as well. You will likely get a lot more mileage out of the microcentrifuge.
- Need a -20 lab freezer as well (NOT a kitchen freezer - don't want that auto-defrost feature). Undercounter fridge and freezer can typically be had second hand for $100-200 each.
 

Best of luck! And remember, this is a marathon, not a sprint...

Patrik

On Saturday, June 27, 2020 at 1:26:32 PM UTC-7, J.R. Logan wrote:
We have been very fortunate to get a state innovation grant approved to fund the establishment of a community/DIY bio space in our makerspace. Informed by the various online equipment lists and my own understanding from talking to bio space organizers at the Community Bio Summit I came up with a proposal for a proposal of $21,700 ($6,000 of Infrastructure, $2,000 of Program expense and $13,700 of Equipment). In the proposal I noted it as a minimum viable budget, and a more full featured lab would cost at another $20,000 or more. The funding committee not only approved the plan but asked to see the list of equipment we would acquire if we received the additional $20,000. 

To meet their request I have compiled a list of items and general expected expenses. The items on the list are representative and we will research the best we can get for the price as we order specific models. I know the specific expenses will work out somewhat differently but that is OK with this funding as long as the overall total equipment expense is close.


I would appreciate it if anyone with experience running a community bio lab would review and comment on my list and proposal. What are my blind spots on this? Anything really important I am missing? Are there certain items where I should buy the best we can afford? Others where it is wasteful to buy, as getting donations is common?  If you think it looks good, let me know. It will be helpful to tell the review committee I have validated my proposal with the wider community of bio space leaders.

J.R. Logan
Executive Director
MakeHaven

JR: 203-676-3414 | LinkedIn | Facebook | Meeting
MakeHaven:  TourNewsletter | Facebook | YouTube | Instagram | SlackTwitter | Pintrest | Github Website

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