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Posted By Sheroo to **Virtual University Of Pakistan**Student Cafe at 7/31/2012 11:41:00 PM

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[DIYbio] Re: Bacteria Lava-Lamp

Im sorry, I do have the plasmid map. It seems that he was referring to the attached document he had sent in the email but I never saw it. I have attached the plasmid map to my post. 

On Tuesday, July 31, 2012 11:19:26 PM UTC-4, Chowe wrote:

That is great to hear! I have also plated some glowing cells on amp plates (my first at home experiment) and I finally got them to glow. It was a great feeling when I first saw them. Now If I were to put these plates in the freezer to keep for a while, how long do you think I could save them for later use?

Also, I got an email back from the supplier of the gram-positive glowing plasmid and they said that there is information on the plasmid map on the site, he wasn't too clear about it. He also added that it is about $561 US for the plasmid, not very cheap.  

On Monday, July 30, 2012 11:18:48 AM UTC-4, Mega wrote:
Just wanted to tell,

I plated the glowing colis (from my bio fridge) on new plates, and they were much brighter than the original ones. Maybe because of less satellite colonies because of fresh amp ?
They were even so bright that you could see their glowing in a semi-dark room (10 o clock PM, streetlights illuminating the room weakly) without adapting your eyes to it.




Am Sonntag, 22. Juli 2012 01:15:33 UTC+2 schrieb Chowe:
Hello! Im very new to DIYbio and I am looking to do an exciting first project. I work in a genetics lab so I have experience with most techniques. I have had many ideas for projects, that I happily found out most people have had the same ones too (glowing plants, glowing yogurt) but I thought a lava lamp would be the simplest way. I am wondering how you guys would go about completing this. I saw that cambridge igem team made a lava-lamp, seen here http://www.youtube.com/watch?v=tUFscEVK5Ks I would like to make something just like that. I would prefer if I didn't have to induce it or if I did it would be through oxygen like the cambridge lava-lamp. It would be cool to have in my room to light it up at night. As with the glowing plants and yogurt they seem to be a lot harder and it seems like not many people have been successful so I wanted to choose a project that I would be more likely to succed. Thanks!

Corey

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[DIYbio] Re: Bacteria Lava-Lamp

That is great to hear! I have also plated some glowing cells on amp plates (my first at home experiment) and I finally got them to glow. It was a great feeling when I first saw them. Now If I were to put these plates in the freezer to keep for a while, how long do you think I could save them for later use?


Also, I got an email back from the supplier of the gram-positive glowing plasmid and they said that there is information on the plasmid map on the site, he wasn't too clear about it. He also added that it is about $561 US for the plasmid, not very cheap.  

On Monday, July 30, 2012 11:18:48 AM UTC-4, Mega wrote:
Just wanted to tell,

I plated the glowing colis (from my bio fridge) on new plates, and they were much brighter than the original ones. Maybe because of less satellite colonies because of fresh amp ?
They were even so bright that you could see their glowing in a semi-dark room (10 o clock PM, streetlights illuminating the room weakly) without adapting your eyes to it.




Am Sonntag, 22. Juli 2012 01:15:33 UTC+2 schrieb Chowe:
Hello! Im very new to DIYbio and I am looking to do an exciting first project. I work in a genetics lab so I have experience with most techniques. I have had many ideas for projects, that I happily found out most people have had the same ones too (glowing plants, glowing yogurt) but I thought a lava lamp would be the simplest way. I am wondering how you guys would go about completing this. I saw that cambridge igem team made a lava-lamp, seen here http://www.youtube.com/watch?v=tUFscEVK5Ks I would like to make something just like that. I would prefer if I didn't have to induce it or if I did it would be through oxygen like the cambridge lava-lamp. It would be cool to have in my room to light it up at night. As with the glowing plants and yogurt they seem to be a lot harder and it seems like not many people have been successful so I wanted to choose a project that I would be more likely to succed. Thanks!

Corey

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Re: [DIYbio] Re: Artificial Jellyfish

Oh, that is *very* clever. My apologies, I was only running off what I
saw in the email thread! :)

On 31/07/12 22:04, Nathan McCorkle wrote:
> On Tue, Jul 31, 2012 at 4:59 PM, Cathal Garvey <cathalgarvey@gmail.com> wrote:
>> IMHO, Sugar would make a poor substrate for cell culture. The osmotic
>> pressure of growing on sugar would be huge; that's one reason why it's
>> traditional to add so much sugar to preserves like jam.
>>
>
> Cathal, they don't use the sugar to grow on, they print a mold with
> the sugar, then add gelatin + cells and pour it over the mold. Once
> the gelatin hardens, the mass is rinsed in an orbital shaker with
> media or buffer (not sure which) to dissolve the sugar, then that
> liquid is replaced with media. In Jordan's case, the mold looks like
> vasculature to aid perfusion later (he perfuses with orbital shaking,
> but I think he's also working on using a pump driven system).
>

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Re: [DIYbio] Re: Artificial Jellyfish

On Tue, Jul 31, 2012 at 4:59 PM, Cathal Garvey <cathalgarvey@gmail.com> wrote:
> IMHO, Sugar would make a poor substrate for cell culture. The osmotic
> pressure of growing on sugar would be huge; that's one reason why it's
> traditional to add so much sugar to preserves like jam.
>

Cathal, they don't use the sugar to grow on, they print a mold with
the sugar, then add gelatin + cells and pour it over the mold. Once
the gelatin hardens, the mass is rinsed in an orbital shaker with
media or buffer (not sure which) to dissolve the sugar, then that
liquid is replaced with media. In Jordan's case, the mold looks like
vasculature to aid perfusion later (he perfuses with orbital shaking,
but I think he's also working on using a pump driven system).

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Re: [DIYbio] Re: Artificial Jellyfish

IMHO, Sugar would make a poor substrate for cell culture. The osmotic
pressure of growing on sugar would be huge; that's one reason why it's
traditional to add so much sugar to preserves like jam.

On 30/07/12 14:07, Jordan Miller wrote:
> here's our sugar extruder:
> http://blog.reprap.org/2012/07/on-challenge-of-3d-printing-sugar-for.html
>
> cheers,
> jordan
>
>
>
> On Jul 30, 2012, at 1:08 AM, "Jeremy[biohak.net]" <jeremya11z@gmail.com>
> wrote:
>
> No I do not. I wish I did. I don't really have access to the type of
> equipment necessary to accomplish that. I am interested though in maybe
> converting a rep rap to do sugar extrusions. Are the home cnc techniques
> that could work for something like this?
>
> On Thursday, July 26, 2012 8:50:59 AM UTC-5, Petfixer71 wrote:
>>
>> Do you have Any experience with cell culture on extracellular matrix??
>>
>>
>>
>> On Monday, July 23, 2012 3:07:40 AM UTC-4, Jeremy[biohak.net] wrote:
>>>
>>> Here's <http://www.biohak.net/node/12>here's an article I posted up on
>>> my blog. Apparently researchers were able to produce this jellyfish by
>>> grown muscle cells along the outer surface of a polymer mold.
>>>
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Re: [DIYbio] Re: Needing some things

For *lab strain* E.coli, my understanding is that they're generally
unregulated unless they're GMO.

At least in Ireland for example, there is a legal framework regulating
Class 2+ bacteria for health and safety reasons. E.coli is in the list,
but with a footnote stating that domesticated lab strains are exempt.

To send E.coli in the post, send a "stab": create a little test tube of
slanted agar, then stab an inoculating loop of E.coli into the agar. You
can cap the tube (E.coli are facultative anaerobes, they won't need air)
and send it in a padded envelope. For added surety, alcohol-spray a
thick plastic ziploc bag, let it dry, put the tube in that before
mailing though. That way, if the tube breaks, the fragments will
possibly still be in a sanitised environment for recovery on the other end.

Label the letter "Non-hazardous biological sample": it's good manners
and could avoid trouble if customs at either end take exception to test
tubes of unidentified bacteria. Ideally, include a little letter slip in
the envelope describing the strain in case customs decide it's worth
opening. I've never heard of one being opened though; labelling it is
pretty "transparent". Postal service workers in most countries haven't
caught the "OMG BIOTERRORISTS" bug yet, it seems. :)

On 31/07/12 16:54, Andreas Sturm wrote:
> Maybe I can try to send you a lab strain from our university....
>
> But I fear, without them frozen they won't survive until the letter'd have
> arrived... Anyway, is it legal to send (genetically unmodified) Colis via
> mail??
>

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[DIYbio] An Engineering Approach to Extending Lifespan in C. elegans.

This article might be of interest to some DIYbio'ers who are also interested in life extension experiments.


Dror Sagi and Stuart Kim, professors at Stanford Medical School wrote: We have taken an engineering approach to extending the lifespan of Caenorhabditis elegans. Aging stands out as a complex trait, because events that occur in old animals are not under strong natural selection. As a result, lifespan can be lengthened rationally using bioengineering to modulate gene expression or to add exogenous components. Here, we engineered longer lifespan by expressing genes from zebrafish encoding molecular functions not normally present in worms. Additionally, we extended lifespan by increasing the activity of four endogenous worm aging pathways. Next, we used a modular approach to extend lifespan by combining components. Finally, we used cell- and worm-based assays to analyze changes in cell physiology and as a rapid means to evaluate whether multi-component transgenic lines were likely to have extended longevity. Using engineering to add novel functions and to tune endogenous functions provides a new framework for lifespan extension that goes beyond the constraints of the worm genome. 

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Re: [DIYbio] Re: Miniprep without EDTA

230nm is used for phenol and related organic detection, it doesn't factor into the 260:280 number

On Jul 31, 2012 7:31 AM, "Slangtry" <sarah.langtry@gmail.com> wrote:
The 260:280 ratio should  actually be as close to 1.8 as possible.  Lower is indicative of too much protein and higher can indicate RNA.  (Could always tell when the RNase was going bad when all the preps were 2.1 or higher.)  Anything (approximately) outside the 1.7 to 2.0 range and it becomes difficult to trust the 230nm reading used to calculate the concentration. 





On Saturday, July 28, 2012 7:09:44 AM UTC-7, Avery wrote:

Iirc as close to 2 as possible.

On Jul 28, 2012 5:38 AM, "Mega" <masterstorm123@gmail.com> wrote:
So, the values are:

For the 209.39 ng/uL plasmid  the 260:280 ratio is 1.46
176.51 ng/uL   -> 1.91
54.17 ng/uL  -> 1.74



I think 1.91 is the best? Should it be high or low? ;)





Am Sonntag, 27. Mai 2012 17:54:23 UTC+2 schrieb Mega:
Hello @all,

I was wondering if I could do a dirty miniprep without EDTA.

What I have: weak centrifuge, SLS (sodium lauryl sulfate), NaOH, vinegar, ethanol (I have access to both wodka and pure ethanol), water.


Can you do a mp with this limited resources?
What I want: Some ~60 to 70 % plasmids, some remaining proteins, RNA, mabe some fragments of chromosomal DNA.


I think with this percentage you can still do a transformation with E.Coli? Because Proteins will either be used or digested, RNA my do it's job inside the cell, chromosomal DNA has no origin of replication.

Only the plasmids will be able to replicate and thus you will get some transformants. These you select with ampicillin anyway. (Clearly, you won't get so much transformants, but who cares? )

Will DNAses inside the solution possibly destroy the plasmids??

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[**Virtual University Of Pakistan**Student Cafe] .::VULMSIT::.eNoxel BURMA KE MUSALMAN HAMERY BEHN BAHI APNI DUAOO MAIN YAD RKHAIN



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Re: [DIYbio] Re: Needing some things


And the ''no delivery to residental adresses'' is *often* not meant so seariously... I'd just  try it  ;) 
Don't mention you're doing diy bio, just write it as you were a lab and  would every day try out samples from companies.


Worst case: they don't answer your mail or  even worse, some conservative may tell you you shouldn't do experiments at home (one of the most stupid attitudes ever!). So you just don't answer back and never mind :)
 

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Re: [DIYbio] Re: Needing some things

Maybe I can try to send you a lab strain from our university....

But I fear, without them frozen they won't survive until the letter'd have arrived... Anyway, is it legal to send (genetically unmodified) Colis via mail?? 

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Re: [DIYbio] (transfected) Insect cell -> entire organism

Microinjection?

http://www.youtube.com/watch?v=h-Bfc1GPWpE

Looks cool... But, it will be hard to build it in a diy way... 




2012/7/31 Cathal Garvey <cathalgarvey@gmail.com>
Not so much complicated as extremely difficult. You need a
micromanipulator and microneedle for one thing, and then you need the
manual skill to use one effectively. Finally, you need lots and lots of
insect ova! :)

This is routine for making transgenic fruit flies, for example. I was
interested in trying it with sea monkies (Artemia salina) for a while
until I discovered how hard it is to get microneedles/micromanipulators.
I'm leaving it for later!

On 30/07/12 15:56, Andreas Sturm wrote:
>> theoretically you could also do somatic cell nuclear transfer (SCNT)
>>from a modified cell to an embryo
>
>
> Of course, but this seems even more complicated ;)
>
>
>
> 2012/7/30 Nathan McCorkle <nmz787@gmail.com>
>
>> yes you want to hit the germline to get expression in the whole
>> organism (or at least to get the new DNA in the whole organism, you
>> may add DNA that only expresses in one body area)
>>
>> theoretically you could also do somatic cell nuclear transfer (SCNT)
>> from a modified cell to an embryo
>>
>> On Sun, Jul 29, 2012 at 8:25 AM, Mega <masterstorm123@gmail.com> wrote:
>>> Hey,
>>>
>>> I read that it would be quite easy to add genes to insect cells using the
>>> piggyBac transposon. Frequently used for recombinant protein expression
>> in
>>> insect cell lines.
>>> So I was wondering how you could get an insect out of those cells. Do you
>>> have to transfect insect sperm? Insects are not plants, so you probably
>>> can't use any cell for reproducing an entire organism?
>>>
>>>
>>>
>>>
>>>
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>>
>>
>>
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Email: niranjank@xperttech.com 

Phone: 781-926-0781

Fax: 978-405-5040

www.XpertTech.com

Delivering the POWER of Technology

 

 



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Direct Client: .Net Developer; 12+ months; Camp Hill, PA

Please send your resumes at niranjank@xperttech.com

This is Niranjan from XpertTech; hope you are doing well.

Please go through below description and reply with your resume, contact details and current location, if you feel comfortable

 

ROLE:.Net Developer

Duration: 12+ months

Location: Camp Hill, PA

Interview: Must be able to do skype interview.

 

Required Skills:

·         .NET 3.5

·         Excellent SQL development skills

·         Excellent understanding of Object Oriented Design and Programming

·         Knowledge of SQL DB administration a plus

·         Ability to write use cases

Preferred Skills

 

Role Title

- Developer

Responsibilities

- Converts a design into a complete information system. Includes acquiring and installing systems environment; creating and testing databases; preparing test case procedures; preparing test files; coding, compiling, and refining programs.

EXPECTED DELIVERABLES: Converts a design into a complete information system.

 

 

 

Thanks&Regards,

Niranjan Kumar Chikkala

Technical Recruiter

400 W Cummings Park

Suite#2850

Woburn, MA-01801

Email: niranjank@xperttech.com 

Phone: 781-926-0781

Fax: 978-405-5040

www.XpertTech.com

Delivering the POWER of Technology

 

 


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[**Virtual University Of Pakistan**Student Cafe] .::VULMSIT::.eNoxel CS610 & MGT301 Solved ppr, Attempt Today

Aslamoalakum All,
                           Please find the attachment for Solved CS610 & MGT301 ppr's Attempt Today.


One at 11:00 Am and other One at 2:30PM


Wish you All Best of luck for your future plans ...  Hopefully I am Done with VU  - InnShaaAllah

Stay Bless

Allah Hafiz

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Posted By Sheroo to **Virtual University Of Pakistan**Student Cafe at 7/31/2012 04:48:00 AM

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Tue, 31 Jul 2012 - Today Jobs on all Leading Newspapers of Pakistan

Pakistan Newspaper Jobs on Jang, Nawa e Waqt, Express, Dawn, The Nation, The News Newspapers for date Tue, 31 Jul 2012.

Note: If you use Digest Email Option then HTML formatting will not work well, browse jobs manually from our webiste directly: http://www.paperparho.com/

Allama Iqbal Open University Islamabad Job Correction Notice
Published on Daily Jang Newspaper

Assistant Controller of Examinations and Examination Officer Required for Lahore Institution
Published on Daily Jang Newspaper

BSc Civil Engineer Required for Rawalpindi
Published on Daily Jang Newspaper

Bum Disposal Comander and Bum Disposal Technicinas Required for District Office Civil Deffence Bahawalnagar
Published on Daily Jang Newspaper

Cheif Executive Officer, Assistant Manager Clinical Pharmacology and Other Different Staff Required for Punjab Industrial Estate Development and Management Company Lahore
Published on Daily Jang Newspaper

Different Staff Required from Islamabad and Adjacent Cities
Published on Daily Express Newspaper

Different Staff Required from Lahore and Adjacent Cities
Published on Daily Express Newspaper

Different Staff Required from Lahore and Adjacent Cities 1
Published on Daily Jang Newspaper

Different Staff Required from Lahore and Adjacent Cities 2
Published on Daily Jang Newspaper

Different Staff Required from Lahore and Adjacent Cities 3
Published on Daily Jang Newspaper

Different Staff Required from Rawalpindi and Adjacent Cities 1
Published on Daily Jang Newspaper

Different Staff Required from Rawalpindi and Adjacent Cities 2
Published on Daily Jang Newspaper

Different Staff Required from Rawalpindi and Adjacent Cities 3
Published on Daily Jang Newspaper

Different Technical Staff Required for Abu Dehbi / UAE
Published on Daily Express Newspaper

Different Technical Staff Required for Saudi Arabia 1
Published on Daily Express Newspaper

Different Technical Staff Required for Saudi Arabia 2
Published on Daily Express Newspaper

Different Technical Staff Required for Saudi Arabia 3
Published on Daily Express Newspaper

Different Technical Staff Required for Saudi Arabia 4
Published on Daily Express Newspaper

Different Technical Staff Required for Saudi Arabia 5
Published on Daily Express Newspaper

Different Technical Staff Required for UAE 1
Published on Daily Express Newspaper

Different Technical Staff Required for UAE 2
Published on Daily Express Newspaper

Female Librarian Required for Islamabad Institution Islamabad
Published on Daily Jang Newspaper

Finance Director / Cheif Financial Officer Required for Lahore Electric Supply Company (LESCO)
Published on Daily Express Newspaper

Finance Director / Cheif Financial Officer Required for Lahore Electric Supply Company (LESCO)
Published on Daily Dawn Newspaper

Headmaster / Headmistress Required for Cadet College Larkana
Published on Daily Express Newspaper

Male, Female IT Engineers and Fresh HR, MBA Management Trainee Officers and Other Different Staff Required for Multinational Firm
Published on Daily Jang Newspaper

Managing Director Required for Gilgit Baltistan Council Secretariat Islamabad
Published on Daily Jang Newspaper

Naib Qasid and Security Guards Required for Government College Township Lahore
Published on Daily Express Newspaper

Professors, Associate Profeesors, Assistant Professors, Lecturers, Principal and Other Different Staff Required for Pakistan Institute of Fashion and Design Lahore
Published on Daily Jang Newspaper

Resident Engineer Required for Park View Villas Lahore
Published on Daily Jang Newspaper

Secretary General Required for Gujranwala
Published on Daily Express Newspaper

Senitary Worker and Loader Required for City District Government Rawalpindi
Published on Daily Express Newspaper


Jobs/Study Opportunities in Saudi Arabia, UAE, Bahrain, Canada, UK, Denmark, Australia, Italy, Cyprus, Germany and Other Countries on Sunday Newspapers
Published on Daily Jang Newspaper: Click here to View
Published on Daily Express Newspaper: Click here to View
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Published on The News Newspaper: Click here to View
Published on The Nation Newspaper: Click here to View

You can browse jobs from previous dates from all newspapers by selecting your required date and newspaper:
Click here to View All Old Jobs

Published on All Newspaper


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Re: [DIYbio] (transfected) Insect cell -> entire organism

Not so much complicated as extremely difficult. You need a
micromanipulator and microneedle for one thing, and then you need the
manual skill to use one effectively. Finally, you need lots and lots of
insect ova! :)

This is routine for making transgenic fruit flies, for example. I was
interested in trying it with sea monkies (Artemia salina) for a while
until I discovered how hard it is to get microneedles/micromanipulators.
I'm leaving it for later!

On 30/07/12 15:56, Andreas Sturm wrote:
>> theoretically you could also do somatic cell nuclear transfer (SCNT)
>>from a modified cell to an embryo
>
>
> Of course, but this seems even more complicated ;)
>
>
>
> 2012/7/30 Nathan McCorkle <nmz787@gmail.com>
>
>> yes you want to hit the germline to get expression in the whole
>> organism (or at least to get the new DNA in the whole organism, you
>> may add DNA that only expresses in one body area)
>>
>> theoretically you could also do somatic cell nuclear transfer (SCNT)
>> from a modified cell to an embryo
>>
>> On Sun, Jul 29, 2012 at 8:25 AM, Mega <masterstorm123@gmail.com> wrote:
>>> Hey,
>>>
>>> I read that it would be quite easy to add genes to insect cells using the
>>> piggyBac transposon. Frequently used for recombinant protein expression
>> in
>>> insect cell lines.
>>> So I was wondering how you could get an insect out of those cells. Do you
>>> have to transfect insect sperm? Insects are not plants, so you probably
>>> can't use any cell for reproducing an entire organism?
>>>
>>>
>>>
>>>
>>>
>>> --
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>>> https://groups.google.com/d/msg/diybio/-/90J9--BtBqYJ.
>>> For more options, visit https://groups.google.com/groups/opt_out.
>>>
>>>
>>
>>
>>
>> --
>> Nathan McCorkle
>> Rochester Institute of Technology
>> College of Science, Biotechnology/Bioinformatics
>>
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>>
>>
>>
>

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twitter.com/onetruecathal
joindiaspora.com/u/cathalgarvey
PGP Public Key: http://bit.ly/CathalGKey

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[**Virtual University Of Pakistan**Student Cafe] .::VULMSIT::.eNoxel Yeh Pichlay Ishq Ki Baatein Hein

Yeh Pichlay Ishq Ki Baatein Hein 
Jab Aankh Mein Khuwaab Chamakte The 

Jab Dil Mein Daag Damakte The 
Jab Palken Shaher Ke Raston Par 

Ashkon Ka Noor Luta Ti Thin 
Jab Chand Ki Rim Jhim Kirno Se 

Sochon Mein Bhawanr Parr Jaate The 
Jab Aik Talatum Rehta Tha 

Apne Be Ant Khayalon Mein 
Har Ahad Nibhane Ki Qsmein 

Khat Khoon Se Likhne Ki Rasmein 
Jab Aam Thi Ham Dil Walon Mein 

Yeh Pichley Ishq Ki Baatein Hein

 


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Perhne Ko Phir Wo QURAN Deta Hay
Bakhsne Pe Ata Hai Jab Umaat K Gunhaon Ko
Tofah Main Gunhangaron Ko RAMADAN Deta Hay



Apply Group MemberShip

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Posted By Sheroo to **Virtual University Of Pakistan**Student Cafe at 7/31/2012 03:40:00 AM

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[**Virtual University Of Pakistan**Student Cafe] [~>VU-P!nD!<~] Yeh Pichlay Ishq Ki Baatein Hein

Yeh Pichlay Ishq Ki Baatein Hein 
Jab Aankh Mein Khuwaab Chamakte The 

Jab Dil Mein Daag Damakte The 
Jab Palken Shaher Ke Raston Par 

Ashkon Ka Noor Luta Ti Thin 
Jab Chand Ki Rim Jhim Kirno Se 

Sochon Mein Bhawanr Parr Jaate The 
Jab Aik Talatum Rehta Tha 

Apne Be Ant Khayalon Mein 
Har Ahad Nibhane Ki Qsmein 

Khat Khoon Se Likhne Ki Rasmein 
Jab Aam Thi Ham Dil Walon Mein 

Yeh Pichley Ishq Ki Baatein Hein

 


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Bezabon Ko Jab Wo Zaban Deta Hay
Perhne Ko Phir Wo QURAN Deta Hay
Bakhsne Pe Ata Hai Jab Umaat K Gunhaon Ko
Tofah Main Gunhangaron Ko RAMADAN Deta Hay



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Posted By Sheroo to **Virtual University Of Pakistan**Student Cafe at 7/31/2012 03:40:00 AM

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[**Virtual University Of Pakistan**Student Cafe] [~>VU-P!nD!<~] لمحہ بھر کو ہی سہی


اب تو خواہش ہے کہ یہ زخم بھی کھا کر دیکھیں
لمحہ بھر کو ہی سہی اُس کو بھُلا کر دیکھیں

شہر میں جشنِ شبِ قدر کی ساعتِ آئی
آج ہم بھی تیرے ملنے کی دعا کر دیکھیں

آندھیوں سے جو اُلجھنے کی کسک رکھتے ہیں
اِک دیا تیز ہَوا میں بھی جلا کر دیکھیں

کچھ تو آوارہ ہواؤں کی تھکن ختم کریں
اپنے قدموں کے نشاں آپ مٹا کر دیکھیں

زندگی اب تجھے سوچیں بھی تو دم گُھٹتا ہے
ہم نے چاہا تھا کبھی تجھ سے وفا کر دیکھیں

جن کے ذرّوں میں خزاں ہانپ کے سو جاتی ہے
ایسی قبروں پہ کوئی پھول سجا کر دیکھیں

دیکھنا ہو تو محبت کے عزا داروں کو
ناشناسائی کی دیوار گِرا کر دیکھیں

یوں بھی دنیا ہمیں مقروض کیے رکھتی ہے
دستِ قاتل تیرا احساں بھی اُٹھا کر دیکھیں

رونے والوں کے تو ہمدرد بہت ہیں محسنؔ
ہنستے ہنستے کبھی دنیا کو رُلا کر دیکھیں

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Bakhsne Pe Ata Hai Jab Umaat K Gunhaon Ko
Tofah Main Gunhangaron Ko RAMADAN Deta Hay



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Posted By Sheroo to **Virtual University Of Pakistan**Student Cafe at 7/31/2012 03:36:00 AM

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