[DIYbio] Re: DIYbio events for the week of July 30

Hello,

I'd like to add the Orbella Fragrant Moss launch which I'm hosting on Thursday in San Francisco.We are going to have a showcase/retrospective of biotech/bio-designed consumer products, some 'grown' furniture and of course our Orbella Fragrant Moss, as well as what might be the worlds first autonomous robot DJ (DJ Human), soylent based cocktails, snacks etc.

 

If you are in San Francisco this week please come along. Details:

If you are working on a consumer biotech/bio-design product and want to showcase it as well get in touch. 

 

Antony

 

PS If you want to try the product you can use discount code FRIENDSANDFAMILY


On Thursday, July 27, 2017 at 8:48:12 PM UTC-7, balduino wrote:

Late edits can be found at https://diybio.org/category/events/

Here are your DIYbio events for the week of July 30

Sunday, July 30

Brooklyn, NY, USA – Summer Ferments   Cheryl Paswater guides exploration and craft of two delicious types of vegetable ferments that are easily made at home without any special ingredients or equipment.

Brooklyn, NY, USA – Fungi Fabrication: Mycelium Materials.   Work with mycelium as material for experimental design applications. Grow a variety of samples, starting from a store bought mushroom, to mycelium grown using experimental techniques, to shaping molds to form mycelium composite materials grown to shapee.  In this 3-session course,  Ali Schachtschneider helps you gain skills and insight into potential applications for fungi based materials—walking away with new knowledge, mycelium tissue-culture skills, and grown material samples.

Oakland, CA, USA – Open Insulin Lab Day.  The Open Insulin Project continues.

Ottowa, ON, CAN – Open House! See Ottowa's biohackerspace

Tuesday, August 1

Cambridge, UK Biomakespace Introduction & Tour (for prospective members)

Longwood, FL, USA – Open House

Oakland, CA, USA – Art of Science, Science of Art   Show, tell and share anything that you are interested in relating to the intersection of art with science, math and engineering.

San Diego, CA, USA – Shooting Stars from Mars-Solving the Mystery With Oxygen Isotope Forensics   Learn about how Dr. Robina Shaheen is using chemistry to investigate one of the biggest mysteries of nature- the origin and history of the Red Planet.

Santa Clara, CA, USA – Quantum Biology-Community Project  Work continues on a quantum salinity detector  (Surface Plasmon Salinity Detector)

Wednesday, August 2

Charlottesville, VA, USA – Biohack Meeting  Work on egg fertilization outside the shell, as well as Green Wall and Virtual Reality teaching programs continues

Oakland, CA, USA – Open Source Insulin Project The Open Insulin Project continues

Oakland, CA, USA – FermentationStation MycoFermentoOmniMondo Various fun fermentations of foodstuffs to produce beverages, etc continues.

Santa Clara, CA, USA – Microfluidics: 'Lab on a Chip'    Eric Harness guides development of lab analysis automation hardware at the micro and nano level.

Somerville, MA, USA – Open House

Thursday, August 3

Denver, CO, USA – Guests Speaker: Charlie Kirkham – An Introduction to Ecology! A tour through the powerful world of ecology: its features, its influence on our lives, and the benefits understanding it offers.

Santa Clara, CA, USA – Bioprinter Community Project Night.  The project to develop live cell printing continues.

Seattle, WA, USA – Lightning Science Talks at SoundBio  Six scientists from University of Washington will share stories about how a glowing jellyfish led to a Nobel Prize, why personal data collection is changing the way we view ourselves, how recent technical advances are allowing us to ask new questions in biology, and more.

Friday, August 4

Baltimore, MD, USA – Free Seminar Night! "Black Fungi Getting us to Space" Dr. Cordero!   Radames J. B. Cordero discusses his studies of fungal melanin, and how it might help provide relief from some of the environmental stressors associated with Mars, and perhaps our future planet, as well as other properties and technologies.

Singapore, Singapore – Let's meet to share about improvised lab equipment and home experiments

Saturday, August 5

Brooklyn, NY, USA – Genome Editing with CRISPR-Cas9  Intense hands-on 4-session course to edit the genome of brewers yeast, led by Will Shindel

Cambridge, UK – Water Monitoring (build a 3-d printed microscope)

Seattle, WA, USA – Heart & Blood Workshop  Learn about blood, some of its functions, how it is moved around in different organisms, and cardiac electrophysiology, with a focus on our own system.  Lecture and lab material are provided to learn blood and transport systems, how to determine your hematocrit, typing your own blood, microscopic evaluation of our white blood cells, origin of heart sounds, use of a stethoscope, dissection of a mammalian four-chambered heart, cardiac electrophysiology, and performing an ECG.

San Diego, CA, USA – Under the Sea and 'Scope: Workshop at the La Jolla Riford Library  A look at sea urchin development, with scientists from the Scripps Institution of Oceanography: You'll get to handle live sea stars and urchins, observe fertilization and development under the microscope, and learn about the research activities at SIO.

Santa Clara, CA, USA – Lab Skills 101: Intro to the Lab  Get introduced to the basics of the working in the Sunnyvale lab.  This is required before taking on lab work there.

Santa Clara, CA, USA – Lecture on Advanced Molecular Detection at the CDC The chief science officer for the CDC's Office of Advanced Molecular Detection, Duncan MacCannel gives what should be quite a talk.

Other events coming up

Sunday, August 6
Brooklyn, NY, USA

Monday, August 7
Brooklyn, NY, USA
Oakland, CA, USA
San Francisco, CA, USA
Santa Clara, CA, USA

Tuesday, August 8
Brooklyn, NY, USA
San Francisco, CA, USA
Santa Clara, CA, USA
Seattle, WA, USA

Saturday, August 12
Brooklyn, NY, USA

Monday, August 14
Brooklyn, NY, USA
Oakland CA, USA

Saturday, August 19
Brooklyn, NY, USA
San Diego, CA, USA

Tuesday, August 22
Brooklyn, NY, USA

Thursday, August 24
Brooklyn, NY, USA
Somerville, MA, USA

Friday August 25 – Saturday August 26
Oakland, CA, USA

Sunday, August 27
Brooklyn, NY, USA
Brooklyn, NY, USA
Cambridge, MA, USA

Friday, September 22 – Sunday, September 24
Cambridge, MA, USA

Tuesday, September 26 (13 weeks) APPLICATION DEADLINE SEPTEMBER 1 Amsterdam, Netherlands

To see when the next event is in your area, check the full list of usual suspects. Don't see one near you? Why not have your own and get it posted here? Tell us about it by emailing eve...@diybio.org. It would be great to post some photos of your event somewhere. We'd love to see them. We do have some guidelines for what is posted, and are not capturing every event in the community.


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Re: [DIYbio] Plant Tissue Culture - White growths?

Just curious. What growth hormones are you using?

-SG

On Sun, Jul 30, 2017 at 2:20 PM Sebastian Cocioba <scocioba@gmail.com> wrote:
Firable callus with some browning due to oxidation

On Sat, Jul 22, 2017 at 2:31 AM, Jeff Backstrom <jbackstrom2@gmail.com> wrote:

It's callus.



On Thu, Jul 20, 2017 at 12:25 PM, MC <markyching@gmail.com> wrote:
I've been seeing this chunky, whitish growth on many of my shoot tip cultures. Does anyone know what it is? It doesn't seem to affect the culture and grows too slow to be a contaminant. My best guess is maybe chemical precipitate or odd callus growth of some sort?

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Re: [DIYbio] E. coli with pVIB not glowing, why?

Did you grow it at 37C or 30C? 37 deactivates the enzyme

On Sunday, February 26, 2017 at 12:35:16 PM UTC-5, Nathan McCorkle wrote:


On Feb 25, 2017 9:56 AM, "MC" <marky...@gmail.com> wrote:
>
> That's really interesting, I'm going to have to do some reading on recombination. Both my cultures were DH10B.

Oh, ok, I guess the-odin changed how the sell pVIB... their product page showed this week that they ship pVIB in BL21.

Hard to say what could've happened from my end, then.

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Re: [DIYbio] Plant Tissue Culture - White growths?

Firable callus with some browning due to oxidation

On Sat, Jul 22, 2017 at 2:31 AM, Jeff Backstrom <jbackstrom2@gmail.com> wrote:

It's callus.



On Thu, Jul 20, 2017 at 12:25 PM, MC <markyching@gmail.com> wrote:
I've been seeing this chunky, whitish growth on many of my shoot tip cultures. Does anyone know what it is? It doesn't seem to affect the culture and grows too slow to be a contaminant. My best guess is maybe chemical precipitate or odd callus growth of some sort?

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Rate : $50/hr || Exclusive Job Opportunity || Techno-Functional business analyst

Dear Partner,

Hope you are doing great!!

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Looking for an onshore TFL for GBI Reporting with excellent communication skills, excellent business analyst skills, and moderate experience with database technologies (Oracle, Teradata). Tableau experience a plus.

 

Best Regards,

 

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[DIYbio] DIYbio events for the week of July 30

Late edits can be found at https://diybio.org/category/events/

Here are your DIYbio events for the week of July 30

Sunday, July 30

Brooklyn, NY, USA – Summer Ferments   Cheryl Paswater guides exploration and craft of two delicious types of vegetable ferments that are easily made at home without any special ingredients or equipment.

Brooklyn, NY, USA – Fungi Fabrication: Mycelium Materials.   Work with mycelium as material for experimental design applications. Grow a variety of samples, starting from a store bought mushroom, to mycelium grown using experimental techniques, to shaping molds to form mycelium composite materials grown to shapee.  In this 3-session course,  Ali Schachtschneider helps you gain skills and insight into potential applications for fungi based materials—walking away with new knowledge, mycelium tissue-culture skills, and grown material samples.

Oakland, CA, USA – Open Insulin Lab Day.  The Open Insulin Project continues.

Ottowa, ON, CAN – Open House! See Ottowa's biohackerspace

Tuesday, August 1

Cambridge, UK Biomakespace Introduction & Tour (for prospective members)

Longwood, FL, USA – Open House

Oakland, CA, USA – Art of Science, Science of Art   Show, tell and share anything that you are interested in relating to the intersection of art with science, math and engineering.

San Diego, CA, USA – Shooting Stars from Mars-Solving the Mystery With Oxygen Isotope Forensics   Learn about how Dr. Robina Shaheen is using chemistry to investigate one of the biggest mysteries of nature- the origin and history of the Red Planet.

Santa Clara, CA, USA – Quantum Biology-Community Project  Work continues on a quantum salinity detector  (Surface Plasmon Salinity Detector)

Wednesday, August 2

Charlottesville, VA, USA – Biohack Meeting  Work on egg fertilization outside the shell, as well as Green Wall and Virtual Reality teaching programs continues

Oakland, CA, USA – Open Source Insulin Project The Open Insulin Project continues

Oakland, CA, USA – FermentationStation MycoFermentoOmniMondo Various fun fermentations of foodstuffs to produce beverages, etc continues.

Santa Clara, CA, USA – Microfluidics: 'Lab on a Chip'    Eric Harness guides development of lab analysis automation hardware at the micro and nano level.

Somerville, MA, USA – Open House

Thursday, August 3

Denver, CO, USA – Guests Speaker: Charlie Kirkham – An Introduction to Ecology! A tour through the powerful world of ecology: its features, its influence on our lives, and the benefits understanding it offers.

Santa Clara, CA, USA – Bioprinter Community Project Night.  The project to develop live cell printing continues.

Seattle, WA, USA – Lightning Science Talks at SoundBio  Six scientists from University of Washington will share stories about how a glowing jellyfish led to a Nobel Prize, why personal data collection is changing the way we view ourselves, how recent technical advances are allowing us to ask new questions in biology, and more.

Friday, August 4

Baltimore, MD, USA – Free Seminar Night! "Black Fungi Getting us to Space" Dr. Cordero!   Radames J. B. Cordero discusses his studies of fungal melanin, and how it might help provide relief from some of the environmental stressors associated with Mars, and perhaps our future planet, as well as other properties and technologies.

Singapore, Singapore – Let's meet to share about improvised lab equipment and home experiments

Saturday, August 5

Brooklyn, NY, USA – Genome Editing with CRISPR-Cas9  Intense hands-on 4-session course to edit the genome of brewers yeast, led by Will Shindel

Cambridge, UK – Water Monitoring (build a 3-d printed microscope)

Seattle, WA, USA – Heart & Blood Workshop  Learn about blood, some of its functions, how it is moved around in different organisms, and cardiac electrophysiology, with a focus on our own system.  Lecture and lab material are provided to learn blood and transport systems, how to determine your hematocrit, typing your own blood, microscopic evaluation of our white blood cells, origin of heart sounds, use of a stethoscope, dissection of a mammalian four-chambered heart, cardiac electrophysiology, and performing an ECG.

San Diego, CA, USA – Under the Sea and 'Scope: Workshop at the La Jolla Riford Library  A look at sea urchin development, with scientists from the Scripps Institution of Oceanography: You'll get to handle live sea stars and urchins, observe fertilization and development under the microscope, and learn about the research activities at SIO.

Santa Clara, CA, USA – Lab Skills 101: Intro to the Lab  Get introduced to the basics of the working in the Sunnyvale lab.  This is required before taking on lab work there.

Santa Clara, CA, USA – Lecture on Advanced Molecular Detection at the CDC The chief science officer for the CDC's Office of Advanced Molecular Detection, Duncan MacCannel gives what should be quite a talk.

Other events coming up

Sunday, August 6
Brooklyn, NY, USA

Monday, August 7
Brooklyn, NY, USA
Oakland, CA, USA
San Francisco, CA, USA
Santa Clara, CA, USA

Tuesday, August 8
Brooklyn, NY, USA
San Francisco, CA, USA
Santa Clara, CA, USA
Seattle, WA, USA

Saturday, August 12
Brooklyn, NY, USA

Monday, August 14
Brooklyn, NY, USA
Oakland CA, USA

Saturday, August 19
Brooklyn, NY, USA
San Diego, CA, USA

Tuesday, August 22
Brooklyn, NY, USA

Thursday, August 24
Brooklyn, NY, USA
Somerville, MA, USA

Friday August 25 – Saturday August 26
Oakland, CA, USA

Sunday, August 27
Brooklyn, NY, USA
Brooklyn, NY, USA
Cambridge, MA, USA

Friday, September 22 – Sunday, September 24
Cambridge, MA, USA

Tuesday, September 26 (13 weeks) APPLICATION DEADLINE SEPTEMBER 1 Amsterdam, Netherlands

To see when the next event is in your area, check the full list of usual suspects. Don't see one near you? Why not have your own and get it posted here? Tell us about it by emailing events@diybio.org. It would be great to post some photos of your event somewhere. We'd love to see them. We do have some guidelines for what is posted, and are not capturing every event in the community.


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Re: [DIYbio] New To DIYBio

The gene itself is only about 240 bp, so if you can't find it for sale you might be able to build it yourself from long primers.

On Thu, Jul 27, 2017 at 6:17 PM Skyler Gordon <skgor1@gmail.com> wrote:
If you have the capability to buy plasmids, you can most likely buy one that already has GFP cut into it. Other than that I would ask some educational facilities if you can get a sample of the GFP gene to express yourself. If you can't find a plasmid that has GFP and the expression system you want, you may be able to buy the gene from ThermoFisher.

Many companies that sell plasmids won't let you purchase anything under a 'John-Q Public' account so you'll need a business account, non-profit would be the best way to go.

-SG
On Thu, Jul 27, 2017 at 6:01 PM Matt Tucker <mathew.tucker.1@gmail.com> wrote:


On Thursday, July 27, 2017 at 5:11:56 PM UTC-4, Skyler Gordon wrote:
Have you already obtained Agro. spp.? I would look into that before you start trying to get all the other materials.

-SG
On Thu, Jul 27, 2017 at 12:38 PM Matt Tucker <mathew....@gmail.com> wrote:
After endless research and reading about genetics, I have finally decided to yield to my curiosity, and put some time into the lab. As such, I'm trying to find an affordable source for GFP to be used in a project where, agrobactarium- t. is used to deliver GFP into a plant. Thoughts?

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Re: [DIYbio] New To DIYBio

If you have the capability to buy plasmids, you can most likely buy one that already has GFP cut into it. Other than that I would ask some educational facilities if you can get a sample of the GFP gene to express yourself. If you can't find a plasmid that has GFP and the expression system you want, you may be able to buy the gene from ThermoFisher.

Many companies that sell plasmids won't let you purchase anything under a 'John-Q Public' account so you'll need a business account, non-profit would be the best way to go.

-SG

On Thu, Jul 27, 2017 at 6:01 PM Matt Tucker <mathew.tucker.1@gmail.com> wrote:


On Thursday, July 27, 2017 at 5:11:56 PM UTC-4, Skyler Gordon wrote:
Have you already obtained Agro. spp.? I would look into that before you start trying to get all the other materials.

-SG
On Thu, Jul 27, 2017 at 12:38 PM Matt Tucker <mathew....@gmail.com> wrote:
After endless research and reading about genetics, I have finally decided to yield to my curiosity, and put some time into the lab. As such, I'm trying to find an affordable source for GFP to be used in a project where, agrobactarium- t. is used to deliver GFP into a plant. Thoughts?

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