Re: [DIYbio] Plant Tissue Culture - White growths?


It's callus.



On Thu, Jul 20, 2017 at 12:25 PM, MC <markyching@gmail.com> wrote:
I've been seeing this chunky, whitish growth on many of my shoot tip cultures. Does anyone know what it is? It doesn't seem to affect the culture and grows too slow to be a contaminant. My best guess is maybe chemical precipitate or odd callus growth of some sort?

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Exclusive Opening || Java Developer

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Java developer with 4+ year experience with at least on year of building Spring/Spring Boot application. Technologies and patterns used will be: Apigee, Spring Boot, Java, Object Oriented programming, JUnit, RESTful/SOAP services. Must have knowledge of test driven development, CI/CD processes and microservices.

 

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[DIYbio] DIY Lab Impact Survey

Calling all biohackers / DIYbiologists!

Thank you everyone for being so open and welcoming in this community. I've designed a little survey to gather data on the total impact of community labs as means of giving back. It should take no more than 30 minutes to complete.

This survey is meant to gather data on the community impact of the DIY science labs across the world as a means of showcasing the beneficial activities DIY science spaces impart upon their communities. This data will be collected and disseminated to the community labs that participate as a means of securing funding from local government, philanthropic donors, and other supporters of DIY science. One of the biggest problems facing these community science spaces is not demand, it is stable funding sources and skeptical thoughts surrounding what DIY science labs are able to do. We hope this data helps with that.

If you could fill it out and share it with as many people as possible I would be eternally grateful!

https://docs.google.com/forms/d/e/1FAIpQLSeCkmf7Wwo3Ho2UcVVEl1w9QmyuOXY1d0RMJuvaaWRK1BvZyw/viewform

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[DIYbio] Plant Tissue Culture - White growths?

I've been seeing this chunky, whitish growth on many of my shoot tip cultures. Does anyone know what it is? It doesn't seem to affect the culture and grows too slow to be a contaminant. My best guess is maybe chemical precipitate or odd callus growth of some sort?

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Exclusive Opening || IOS Developer|| Palo, Alto, CA

Dear Partner,

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iOS Position

Palo Alto, CA

6+ Months

 

Swift programming experience preferred 

Objective C also is OK. 

Experience with XCTest, XCUITest and XCode 

Experience building Mocks/Stubs based test architectures 

Hands on experience on iOS platform 

 

 

 

Best Regards,

 

Arpit Arora

Pyramid Consulting, Inc.


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[DIYbio] Re: 200$ Real-Time PCR Thermocycler

Definitely sounds interesting. I am doing RNASeq and checking via qPCR if DNAse digestion of genomic DNA was successful, so it is kust presence/absence of a molecule. I am a little bit concerned about sensitivity though

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[DIYbio] Re: 200$ Real-Time PCR Thermocycler

hi Gabriele,
I think as a "Go-no-Go" would be useful and for that price - awesome.
Sign me on.
Chiu


On Tuesday, July 4, 2017 at 5:13:59 PM UTC-4, Gabriele Borelli wrote:
Dear DIYbio users,

my name is Gabriele Borelli, I am a 25 years old passionate and crazy electronic engineer.
I am developing an easy-to-use real-time PCR thermocycler with four collegues.
Now that we have our first product, we would like to ask feedback to the greatest biohackers community in the world.

Here some features of the thermocycler:

- Easy-to-use and controllable through and App for smartphone and tablet.
- Real-time detection on your samples.
- 96 well.
- Not quantitatively accurate as the top-notch Roche thermocycler, but more than enough precise to reliably discriminate between "DNA has been amplified" and "DNA has NOT been amplified"
- SYBR green is not necessary.
- Price: 200$ App included.

Would you buy a thermocycler with the previous features?

We want to bring to everybody who has interest and passion in studying biology the instruments to do that. 
We had this chance in electronics, why should not Biohackers have it?

Thanks you in advance for sharing your opinion, it has a high value!

Ciao,

Gabriele.

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Re: [DIYbio] Microfluidics of single bacteria?

The joint genome institute does single bacterial cell isolation and growth with microchambers ... They are based in Walnut Creek CA and have very good community outreach programs.

On Jul 18, 2017 12:51 PM, "Ana Santos" <researcher.in.biology@gmail.com> wrote:
Hi DIYbio community!
I'm wondering if anyone is aware of a high-throughput microfluidic chip for long term time-lapse microscopy of individual bacteria.
Most chips I come across are intended to monitor the growth of bacterial communities. I want to monitor individual bacteria.
Thanks in advance for your assistance.
Best,
Ana

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Re: [DIYbio] Microfluidics of single bacteria?

On 07/18/2017 11:33 AM, Ana Santos wrote:
> wondering if anyone is aware of a high-throughput microfluidic chip for long term time-lapse microscopy of individual bacteria.

So, by high throughput, do you mean individuals are tracked and snapshotted, and 1000 of them are in a pipeline?

Sounds difficult to keep them fed. Like a job for an AI cell herder...keeping them separated by air bubbles, stirred, aerated,
food injected, illuminated and snapshot every time interval.

Do you have a budget to develop it if not found for sale?

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Re: [DIYbio] Microfluidics of single bacteria?

Ana Santos <researcher.in.biology@gmail.com> writes:

> Hi DIYbio community!
> I'm wondering if anyone is aware of a high-throughput microfluidic chip for
> long term time-lapse microscopy of individual bacteria.
> Most chips I come across are intended to monitor the growth of bacterial
> communities. I want to monitor individual bacteria.
> Thanks in advance for your assistance.
> Best,
> Ana
>

Ana,

https://www.youtube.com/watch?v=y2rNLRv2t5c

They seem to do cell trapping here.


J.

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Exclusive Opening || ETL Developer with Vertica ||

Dear Partner,

Hope you are doing great!!

Please go through the requirement and let me know if you are having any consultant for this position.

Please share me the profile asap as this is a very hot requirement.

 

Vertica Performance Engineer

Sunnyvale CA

6+ Months

 

Description
Primary Skill: VERTICA, BI and Data Warehousing
5 years + experience
Good Knowledge of Vertica Architecture
Hands-on Vertica Implementations
Experience in Performance Tuning in Vertica.
BI and Data Warehousing
Strong Analytical skills

 

 

 

Best Regards,

 

Arpit Arora

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Re: [DIYbio] Microfluidics of single bacteria?

Hi Ana,

I know someone who did this to watch some Xylella fastidiosa swimming.
This bacteria have a veeeery slow life cycle - and have a low motility as well. So this guy acomplished doing this for long periods of time, in the order of days. He built a single channel mimicking the phloem of trees - this bacteria is phytopathogenic.

If your are interested I could put you in contact.

But first, what you are planning to do? Bacteria swimming competition? (kidding)
Share a bit more what's the big plan!

Best,
Otto


2017-07-18 13:33 GMT-03:00 Ana Santos <researcher.in.biology@gmail.com>:
Hi DIYbio community!
I'm wondering if anyone is aware of a high-throughput microfluidic chip for long term time-lapse microscopy of individual bacteria.
Most chips I come across are intended to monitor the growth of bacterial communities. I want to monitor individual bacteria.
Thanks in advance for your assistance.
Best,
Ana

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[DIYbio] Microfluidics of single bacteria?

Hi DIYbio community!
I'm wondering if anyone is aware of a high-throughput microfluidic chip for long term time-lapse microscopy of individual bacteria.
Most chips I come across are intended to monitor the growth of bacterial communities. I want to monitor individual bacteria.
Thanks in advance for your assistance.
Best,
Ana

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Rate $40-45/hr on C2C Exclusive Opening || Android Developer & IOS Developer

Greetings!!

 

I represent Pyramid Consulting Inc., a leading IT staffing organization. I am part of a team responsible for servicing a major client of ours that is a world leader in IT services. I came across your resume on a job board and feel you would be a good fit for a role I have with one of my direct clients. Please see the Job description below and let me know if you would be interested.

 

 

Android Developer

Palo Alto, CA

6+ Months

 

Java language programming 

Android platform experience 

Building automation on Android platform 

Experience with UI Automator and/or Espresso 

 

 

iOS Position

Palo Alto, CA

6+ Months

 

 

Swift programming experience preferred 

Objective C also is OK. 

Experience with XCTest, XCUITest and XCode 

Experience building Mocks/Stubs based test architectures 

Hands on experience on iOS platform 

 

 

 

Best Regards,

 

Arpit Arora

Pyramid Consulting, Inc.


Desk Phone: 415-943-9386 Email : Arpit.arora@pyramidci.com  |  Linked in: https://www.linkedin.com/in/arpit-arora-55793655  | Webhttp://www.pyramidci.com/staffing-home

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[DIYbio] Re: Has anybody done a scoby project similar to The Thought Emporium

Nice work!

I think that the growth rates you quoted are a little on the low side, but great job showing how simple the process really is.

For keeping the scoby supple .. have you tried silicone oils? Hair polish is usually cyclopentasiloxane and dimethicone. They seem to penetrate the material well, and seem to last a long time without any noticeable drying.

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[DIYbio] Re: Has anybody done a scoby project similar to The Thought Emporium


https://www.youtube.com/watch?v=Ds8ZFzOwGeI

https://www.youtube.com/watch?v=PsvbUNTcUoo


https://www.youtube.com/watch?v=q9W98zgMsrE

Here is an update

On Thursday, June 8, 2017 at 7:55:23 PM UTC-7, Kabing wrote:
https://www.youtube.com/watch?v=Ds8ZFzOwGeI

So far I am doing my own batch and I just started the growth process. 






Heres my Project so far though. This Scoby Project was for Benicia Makerspace. 


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Urgent Client Need: Release Engineer; Boston MA; Long Term (Locals Only)

Please send your resumes at niranjan.kumar@vision3solutions.com

 

Hi

 

This is Niranjan from Vision3solutions; hope you are doing well.

Please go through below description and reply with your resume, contact details and current location, if you feel comfortable

 

 

Title: Release Engineer (TFS)

Location; Boston MA

Duration: Long Term

 

Interview: Telephonic AND Face to Face

 

Locals Only

 

Job Responsibilities:

  • Programmatically automate server configuration, software builds, testing and deployments
  • Work with development teams providing deployment services from initial development through production deployments
  • Continuously improve and refactor the tools and code our team builds and maintains
  • Participate in regular after-hours and weekend production deployments
  • Document work for cross-training purposes within the team and craft partner documentation and training
  • Work with various teams (DEV, QA, MIS, PMO, Product) in a cross-functional manner
  • Regularly attend operations team meetings and project scrum meetings


Experience and proficiency with a variety of System tools including:

  • 2+ years experience in automation scripting/programming (PowerShell, Bash, Groovy, Python)
  • Experience using version control (SVN, TFS,) in a collaborative code environment
  • Experience with server configuration automation (Salt, Chef, Puppet)
  • Experience with continuous integration toolsets (Jenkins, TFS,)
  • Experience troubleshooting code compile issues and exceptions (often unfamiliar code)

 

Thanks& Regards

 

Niranjan Kumar

 

 

Vision3 Solutions Inc

8155 Cavendish Place,Suwanee, GA 30024
Contact No:
(678) 554-4785
Email: niranjan.kumar@vision3solutions.com


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Urgent Client Need: Release Engineer; Boston MA; Long Term (Locals Only)

Please send your resumes at niranjan.kumar@vision3solutions.com

 

Hi

 

This is Niranjan from Vision3solutions; hope you are doing well.

Please go through below description and reply with your resume, contact details and current location, if you feel comfortable

 

 

Title: Release Engineer (TFS)

Location; Boston MA

Duration: Long Term

 

Interview: Telephonic AND Face to Face

 

Locals Only

 

Job Responsibilities:

  • Programmatically automate server configuration, software builds, testing and deployments
  • Work with development teams providing deployment services from initial development through production deployments
  • Continuously improve and refactor the tools and code our team builds and maintains
  • Participate in regular after-hours and weekend production deployments
  • Document work for cross-training purposes within the team and craft partner documentation and training
  • Work with various teams (DEV, QA, MIS, PMO, Product) in a cross-functional manner
  • Regularly attend operations team meetings and project scrum meetings


Experience and proficiency with a variety of System tools including:

  • 2+ years experience in automation scripting/programming (PowerShell, Bash, Groovy, Python)
  • Experience using version control (SVN, TFS,) in a collaborative code environment
  • Experience with server configuration automation (Salt, Chef, Puppet)
  • Experience with continuous integration toolsets (Jenkins, TFS,)
  • Experience troubleshooting code compile issues and exceptions (often unfamiliar code)

 

Thanks& Regards

 

Niranjan Kumar

 

 

Vision3 Solutions Inc

8155 Cavendish Place,Suwanee, GA 30024
Contact No:
(678) 554-4785
Email: niranjan.kumar@vision3solutions.com

                 niruk5503@gmail.com

 


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Re: [DIYbio] DIY Laminar flow hood

On 07/13/2017 01:34 AM, Matt Brown wrote:
> I was wondering if anyone had some advice on putting together a laminar flow hood for sterile work?

Sebastian has some opinions on this.

https://groups.google.com/forum/?utm_medium=email&utm_source=footer#!searchin/diybio/hepa|sort:relevance/diybio/9-A-EHhJxN4/PYVnNiUrMK8J

There's lots discussed here that you find by searching
https://groups.google.com/forum/?utm_medium=email&utm_source=footer#!searchin/diybio/laminar$20flow%7Csort:relevance

https://groups.google.com/forum/?utm_medium=email&utm_source=footer#!searchin/diybio/hepa%7Csort:relevance

I'll be developing a UPS shippable low cost positive pressure HEPA flow bench to sell in a while -- too busy with the
culture shock electroporator right now though. (blog.kitmatic.com and https://cibolo.us/mailman/listinfo/open_electroporator)

The most important things seem to be:

1. Avoid vortices in air flow by using squirrel cage fans, baffles, perforated flow source walls,
straight path out the front door.

2. Use a front screen that raises and lowers to reduce the air flow needed, and that is clear see through and cleanable.

3. Make a control for air flow that adjusts to a good low flow that won't blow on your subjects,
dry them out, blow them around, etc.

4. Make a perfect seal to your HEPA filter element edges.

5. Have decent light sources built in because you cannot snake them in and have good cleanliness.

6. Add other niceties like indicators, carry handles

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[DIYbio] DIY Laminar flow hood

I'm trying to put together the equipment I need to start on a DIYBio project, and I was wondering if anyone had some advice on putting together a laminar flow hood for sterile work?  I have plenty of space to set it up, but I not sure what's the best way to construct it or what kind of filters to use.  Any help would be appreciated.

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Position : FullStack Developer (Website Developer) , Hayward, CA

Hi,

 

Please find the below position and respond back to me with your consultants Profiles.

 

Position : FullStack Developer (Website Developer)

Location: Hayward, CA

Duration : 6+ Months

 

Qualification :

 

We need a website developer with

  • FullStack experience including SQL and Java in depth on the backend.
  • Experience including Angular/backbone/bootstrap on the frontend.
  • Bachelors in Computer science or relevant education / experience

 

-


Thanks and Best Regards,

 

Praveen Kumar

SRI Tech Solutions Inc.

praveen@sritechsolutions.com

(T) 1-813-423-6500 X Ext: 149 (M) 248-462 -7719 (F) 813.423.6520

 

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Re: [DIYbio] Re: A simple and cheap >100V DIY electrophoresis power supply

Awesome documentation. Congratz!

2017-07-11 19:43 GMT-03:00 Abizar Lakdawalla <abizarl@gmail.com>:
Old discussion, but I finally built an electrophoresis power supply similar in concept to Josh's idea. Details on the build at instructables.
https://www.instructables.com/id/DIY-Power-Supply-for-Gel-Electrophoresis/

On Wednesday, July 3, 2013 at 9:58:26 AM UTC-7, Josiah Zayner wrote:
Using a bridge rectifier($0.30) one can basically use AC mains voltage to run your gel electrophoresis. You can buy all the components for under $5.
I tested it out and it works great. Can run a really good looking gel in around 30 minutes.

I made a blog post here about it:
http://doitourselfscience.blogspot.com/2013/07/a-cheap-simple-diy-electrophoresis.html


Any questions let me know.


Josiah Zayner

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[DIYbio] Re: A simple and cheap >100V DIY electrophoresis power supply

Old discussion, but I finally built an electrophoresis power supply similar in concept to Josh's idea. Details on the build at instructables.
https://www.instructables.com/id/DIY-Power-Supply-for-Gel-Electrophoresis/

On Wednesday, July 3, 2013 at 9:58:26 AM UTC-7, Josiah Zayner wrote:
Using a bridge rectifier($0.30) one can basically use AC mains voltage to run your gel electrophoresis. You can buy all the components for under $5.
I tested it out and it works great. Can run a really good looking gel in around 30 minutes.

I made a blog post here about it:
http://doitourselfscience.blogspot.com/2013/07/a-cheap-simple-diy-electrophoresis.html


Any questions let me know.


Josiah Zayner

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Position : FullStack Developer (Website Developer) , Hayward, CA

Hi,

 

Please find the below position and respond back to me with your consultants Profiles.

 

Position : FullStack Developer (Website Developer)

Location: Hayward, CA

Duration : 6+ Months

 

Qualification :

 

We need a website developer with

  • FullStack experience including SQL and Java in depth on the backend.
  • Experience including Angular/backbone/bootstrap on the frontend.
  • Bachelors in Computer science or relevant education / experience

 

 

 

Thanks and Best Regards,

 

Praveen Kumar

SRI Tech Solutions Inc.

praveen@sritechsolutions.com

(T) 1-813-423-6500 X Ext: 149 (M) 248-462 -7719 (F) 813.423.6520

 

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