[DIYbio] Re: New plant transformation silver bullet

Also not sure if it would work, the e coli would have to get into the zygote at the right time. I am trying to locate a conjugative e coli (S17) to do some tests with.

On Saturday, March 30, 2019 at 10:28:52 AM UTC-4, Andreas "Mega" Stuermer wrote:
That also sounds cool... However, not sure if it will be able to do real plants? Maybe worth a try?

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[DIYbio] Re: New plant transformation silver bullet

That also sounds cool... However, not sure if it will be able to do real plants? Maybe worth a try?

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Re: [DIYbio] Funded PhD position: Citizen Science & Peer Production

Thanks so much, we'll work openly and collaboratively with the community, so hopefully there'll be news much earlier than after the wrap up of a thesis! 


And the news of me joining the institute is so fresh that there's no lab page yet! For now the best way to keep up is through my own website at https://tzovar.as/ or on Twitter https://twitter.com/gedankenstuecke/. I'll update once we got a proper page set up. :-)

Cheers,
Bastian

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On 29. Mar 2019, at 09:47, Dakota Hamill <dkotes@gmail.com> wrote:

Pretty cool to see PhD positions offered for stuff like this.  Wish you the best in your research and hope you find an eager mind! Share the thesis in a few years!  Do you have a laboratory page where you post research updates?  I saw the institute and job posting but not a PI lablink. 

On Fri, Mar 29, 2019 at 12:45 PM 'Bastian Greshake Tzovaras' via DIYbio <diybio@googlegroups.com> wrote:
Thanks Jason! I definitely plan to take advantage of all of their great lab spaces, incl. the MOOC Factory! 

Thomas and I know each other, indeed: We just ran into each other last week at the CRI! :D 

Cheers,
Bastian 

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While I may be sending this email outside my normal office hours, I have no expectation to receive a reply outside yours.

On 29. Mar 2019, at 09:13, Jason Bobe <jasonbobe@gmail.com> wrote:

Congratulations Bastian -- CRI is an amazing place! Can't wait to see you do there! Hopefully you'll make at least one broadcast from their MOOC Factory :)

(You probably already know Thomas Landrain; he'll be a good contact for you in Paris. By chance if you don't know him, let me know offline and I'm happy to connect you.)

On Fri, Mar 29, 2019 at 11:29 AM 'Bastian Greshake Tzovaras' via DIYbio <diybio@googlegroups.com> wrote:
Hey everyone,
I will join the CRI in Paris (https://cri-paris.org/) this September as a long-term fellow to study how the principles of commons-based peer-production can be used to improve participant involvement and empowerment in citizen science projects. And the best part: I have funding for a PhD student to work on this alongside me! 

The position is funded for 3 years and will start in September 2019. The application deadline is already in mid-April, so things need to move rather fast. If you (or someone you know) might be interested in this: More information about the position can be found at https://tzovar.as/jobs/ 

Please feel free to get in touch if you have any questions regarding the position etc. :-) 

Cheers,
Bastian

--
mobile phone + 1 (510) 944-4298 πŸ‡ΊπŸ‡Έ
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Re: [DIYbio] Funded PhD position: Citizen Science & Peer Production

Pretty cool to see PhD positions offered for stuff like this.  Wish you the best in your research and hope you find an eager mind! Share the thesis in a few years!  Do you have a laboratory page where you post research updates?  I saw the institute and job posting but not a PI lablink. 

On Fri, Mar 29, 2019 at 12:45 PM 'Bastian Greshake Tzovaras' via DIYbio <diybio@googlegroups.com> wrote:
Thanks Jason! I definitely plan to take advantage of all of their great lab spaces, incl. the MOOC Factory! 

Thomas and I know each other, indeed: We just ran into each other last week at the CRI! :D 

Cheers,
Bastian 

--
mobile phone + 1 (510) 944-4298 πŸ‡ΊπŸ‡Έ
mobile phone +49 (176) 213 044 66 πŸ‡ͺπŸ‡Ί

While I may be sending this email outside my normal office hours, I have no expectation to receive a reply outside yours.

On 29. Mar 2019, at 09:13, Jason Bobe <jasonbobe@gmail.com> wrote:

Congratulations Bastian -- CRI is an amazing place! Can't wait to see you do there! Hopefully you'll make at least one broadcast from their MOOC Factory :)

(You probably already know Thomas Landrain; he'll be a good contact for you in Paris. By chance if you don't know him, let me know offline and I'm happy to connect you.)

On Fri, Mar 29, 2019 at 11:29 AM 'Bastian Greshake Tzovaras' via DIYbio <diybio@googlegroups.com> wrote:
Hey everyone,
I will join the CRI in Paris (https://cri-paris.org/) this September as a long-term fellow to study how the principles of commons-based peer-production can be used to improve participant involvement and empowerment in citizen science projects. And the best part: I have funding for a PhD student to work on this alongside me! 

The position is funded for 3 years and will start in September 2019. The application deadline is already in mid-April, so things need to move rather fast. If you (or someone you know) might be interested in this: More information about the position can be found at https://tzovar.as/jobs/ 

Please feel free to get in touch if you have any questions regarding the position etc. :-) 

Cheers,
Bastian

--
mobile phone + 1 (510) 944-4298 πŸ‡ΊπŸ‡Έ
mobile phone +49 (176) 213 044 66 πŸ‡ͺπŸ‡Ί

While I may be sending this email outside my normal office hours, I have no expectation to receive a reply outside yours.

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Re: [DIYbio] Funded PhD position: Citizen Science & Peer Production

Thanks Jason! I definitely plan to take advantage of all of their great lab spaces, incl. the MOOC Factory! 


Thomas and I know each other, indeed: We just ran into each other last week at the CRI! :D 

Cheers,
Bastian 

--
mobile phone + 1 (510) 944-4298 πŸ‡ΊπŸ‡Έ
mobile phone +49 (176) 213 044 66 πŸ‡ͺπŸ‡Ί

While I may be sending this email outside my normal office hours, I have no expectation to receive a reply outside yours.

On 29. Mar 2019, at 09:13, Jason Bobe <jasonbobe@gmail.com> wrote:

Congratulations Bastian -- CRI is an amazing place! Can't wait to see you do there! Hopefully you'll make at least one broadcast from their MOOC Factory :)

(You probably already know Thomas Landrain; he'll be a good contact for you in Paris. By chance if you don't know him, let me know offline and I'm happy to connect you.)

On Fri, Mar 29, 2019 at 11:29 AM 'Bastian Greshake Tzovaras' via DIYbio <diybio@googlegroups.com> wrote:
Hey everyone,
I will join the CRI in Paris (https://cri-paris.org/) this September as a long-term fellow to study how the principles of commons-based peer-production can be used to improve participant involvement and empowerment in citizen science projects. And the best part: I have funding for a PhD student to work on this alongside me! 

The position is funded for 3 years and will start in September 2019. The application deadline is already in mid-April, so things need to move rather fast. If you (or someone you know) might be interested in this: More information about the position can be found at https://tzovar.as/jobs/ 

Please feel free to get in touch if you have any questions regarding the position etc. :-) 

Cheers,
Bastian

--
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Re: [DIYbio] Funded PhD position: Citizen Science & Peer Production

Congratulations Bastian -- CRI is an amazing place! Can't wait to see you do there! Hopefully you'll make at least one broadcast from their MOOC Factory :)

(You probably already know Thomas Landrain; he'll be a good contact for you in Paris. By chance if you don't know him, let me know offline and I'm happy to connect you.)

On Fri, Mar 29, 2019 at 11:29 AM 'Bastian Greshake Tzovaras' via DIYbio <diybio@googlegroups.com> wrote:
Hey everyone,
I will join the CRI in Paris (https://cri-paris.org/) this September as a long-term fellow to study how the principles of commons-based peer-production can be used to improve participant involvement and empowerment in citizen science projects. And the best part: I have funding for a PhD student to work on this alongside me! 

The position is funded for 3 years and will start in September 2019. The application deadline is already in mid-April, so things need to move rather fast. If you (or someone you know) might be interested in this: More information about the position can be found at https://tzovar.as/jobs/ 

Please feel free to get in touch if you have any questions regarding the position etc. :-) 

Cheers,
Bastian

--
mobile phone + 1 (510) 944-4298 πŸ‡ΊπŸ‡Έ
mobile phone +49 (176) 213 044 66 πŸ‡ͺπŸ‡Ί

While I may be sending this email outside my normal office hours, I have no expectation to receive a reply outside yours.

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[DIYbio] Funded PhD position: Citizen Science & Peer Production

Hey everyone,

I will join the CRI in Paris (https://cri-paris.org/) this September as a long-term fellow to study how the principles of commons-based peer-production can be used to improve participant involvement and empowerment in citizen science projects. And the best part: I have funding for a PhD student to work on this alongside me! 

The position is funded for 3 years and will start in September 2019. The application deadline is already in mid-April, so things need to move rather fast. If you (or someone you know) might be interested in this: More information about the position can be found at https://tzovar.as/jobs/ 

Please feel free to get in touch if you have any questions regarding the position etc. :-) 

Cheers,
Bastian

--
mobile phone + 1 (510) 944-4298 πŸ‡ΊπŸ‡Έ
mobile phone +49 (176) 213 044 66 πŸ‡ͺπŸ‡Ί

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Urgent Client Need- Java Developer with Micro Services- Plano TX 12+ Months

Please send your resumes at harish.bioinfosystems@gmail.com

 

Hi

 

This is Harish from BioinfoSystems; hope you are doing well.

Please go through below description and reply with your resume, contact details and current location, if you feel comfortable


Title:Java Developer
with Micro Services

Location:Plano TX

Duration:12+ Months

 

Responsibilities:

·         This role requires a wide variety of strengths and capabilities, including:

·         BS/BA degree or equivalent experience

·         Expertise in application, data and infrastructure architecture disciplines

·         Advanced knowledge of architecture, design and business processes

·         Experience in Java, Gaia Deployments, Microservice design, API Design and Deployment, Kafka or Event architecture likeMQ, Cassandra, Hystrix (circuit breaker patterns), Dynatrace, Splunk and Zipkin and Security pattern implementations

·         Ability to work collaboratively in teams and develop meaningful relationships to achieve common goals

·         Hands on experience with Automation development using Selenium, Cucumber, Gherkin, SOAP/Rest services, DB2, Cassandra and Hadoop Stack (Hive, Spark), CI tools such as Jenkins / Bamboo

·         Can build and understand requirements and business modeling such as class diagram and activity/sequence diagram

·         Translate functional and technical Stories Tech / Use Cases / Acceptance Criteria (corresponding to requirements) into Gherkin Test Scenarios and re-factor the same as needed.

 

Thanks & Regards

Rokkam Harish

Bioinfo Systems LLC

Hartford CT

harish.bioinfosystems@gmail.com

Phone: 860-967-0614


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Urgent Client Need- Java Developer with Micro Services- Plano TX 12+ Months

Please send your resumes at harish.bioinfosystems@gmail.com

 

Hi

 

This is Harish from BioinfoSystems; hope you are doing well.

Please go through below description and reply with your resume, contact details and current location, if you feel comfortable


Title:Java Developer
with Micro Services

Location:Plano TX

Duration:12+ Months

 

Responsibilities:

·         This role requires a wide variety of strengths and capabilities, including:

·         BS/BA degree or equivalent experience

·         Expertise in application, data and infrastructure architecture disciplines

·         Advanced knowledge of architecture, design and business processes

·         Experience in Java, Gaia Deployments, Microservice design, API Design and Deployment, Kafka or Event architecture likeMQ, Cassandra, Hystrix (circuit breaker patterns), Dynatrace, Splunk and Zipkin and Security pattern implementations

·         Ability to work collaboratively in teams and develop meaningful relationships to achieve common goals

·         Hands on experience with Automation development using Selenium, Cucumber, Gherkin, SOAP/Rest services, DB2, Cassandra and Hadoop Stack (Hive, Spark), CI tools such as Jenkins / Bamboo

·         Can build and understand requirements and business modeling such as class diagram and activity/sequence diagram

·         Translate functional and technical Stories Tech / Use Cases / Acceptance Criteria (corresponding to requirements) into Gherkin Test Scenarios and re-factor the same as needed.

 

Thanks & Regards

Rokkam Harish

Bioinfo Systems LLC

Hartford CT

harish.bioinfosystems@gmail.com

Phone: 860-967-0614

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[DIYbio] Re: New plant transformation silver bullet

I am working on getting E. coli conjugation going with unicellular eukaryotes such as  and https://www.sciencedirect.com/science/article/pii/S2211926418310919. Any interest in this approach?

On Sunday, March 24, 2019 at 2:05:23 PM UTC-4, Andreas "Mega" Stuermer wrote:
Hi guys, 

Seems there are new plant transformation kids on the block. It seems that all you have to do in order to get transgenic plants, you can decoat the seeds and put agrobacterium onto it. Then select on kanamycin and you get your transgenic plant. 


Agrobacterium tumefaciens-mediated in planta seed transformation strategy in sugarcane.



Now the products of agrobacterium are regulated in the US. To try out an alternative route that doesn't fall under the plant pest regulation, I'm gathering community labs in the US (and any other nation where this is legal) to try out a new method based on this. 

The basic idea is to use carbon-nanotube+PEI complexes to get DNA into plant cells as described in the paper "High aspect ratio nanomaterials enable delivery of functional genetic material without DNA integration in mature plants" by Demirer et al. 2019.

The DNA gets into the seed cells, as it would with agrobacterium.  But it doesn't integrate, so we employ a transposon to make the gene-of-interest jump into a plant chromosome. 


This is like a Independent-Bio-crowdsourcing project. I guess pretty open source, because maybe life should be about more than making products. 

Everybody who wants to participate, lmk. We are group-ordering PEI and CNT (pretty affordably) and will get the plasmids synthesized together too. If we get a few more people, PEI will be 50% off in bulk. It's 95$ for 5mL (way too much mL to ever use probably) without bulk discount.

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[DIYbio] Re: DiY Plasmid construction and yeast tranformation

The ODIN sells a yeast transformation kit http://www.the-odin.com/ge-yeast/, they are a DIYbio focused company . If you use my coupon code learn there is a 10% discount.

On Wednesday, March 6, 2019 at 2:25:57 PM UTC-5, Jim Wool wrote:
Hello I am starting a new project and it involves taking plasmids with genes that are not present in the yeast genome and transforming them inside. I've been doing some reading and I have the sequences that I intend on using but I was curious if there is a better place to purchase plasmids than the ones on the top of Google results. Also if you folks have any input I would gladly appreciate it.

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Re: [DIYbio] Re: Question from an amateur about laminar flow hood

What questions do you have about laminar flow hoods?

On Mar 28, 2019, at 3:40 PM, MikeCZ <ondra.majer@gmail.com> wrote:

Hello, do you have experience with this laminar flow cabinets?

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<FBB120.jpg>

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[DIYbio] Re: Question from an amateur about laminar flow hood

Hello, do you have experience with this laminar flow cabinets?

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[DIYbio] Invitation to Participate in Research

Sent on behalf of the researcher

Dear Prospective Participants, 

This is an invitation to participate in a study concerning how "expertise" is defined and identified in citizen science projects. We are looking to interview citizen scientists, and researchers running citizen science projects. 

The purpose of the research is to understand how individuals involved in citizen science assess their expertise and the competencies of their peers in terms. The goal of this research is to better understand the implicit and explicit assessment of expertise that STEM researchers use in multidisciplinary collaboration. Understanding these mechanisms has significance to training initiatives at local and national levels. 

Participation in this study is voluntary. If you choose to participate in this study, you will be asked to participate in a 30-minute interview with Dr. Mehlenbacher or a member of her Networked Expertise research team. For your time, you will receive a $5.00 Amazon gift card. 


This study has been reviewed and received ethics clearance through a University of Waterloo Research Ethics Committee (ORE# 21110). If you have questions for the Committee contact the Office of Research Ethics, at 1-519-888-4567 ext. 36005 or ore-ceo@uwaterloo.ca

For all other questions please contact the project PI, Dr. Ashley Rose Mehlenbacher, at ashley.mehlenbacher@uwaterloo.ca

Thank you in advance for your consideration and assistance. 

Cordially, 
Ashley 

Dr. Ashley Rose Mehlenbacher, Assistant Professor, 
Department of English Language and Literature, 
Hagey Hall of the Humanities, room 146 
University of Waterloo, 200 University Ave. West, 
Waterloo, Ontario, Canada N2L 3G1 
+1 519 888 4567 (ext. 39135)

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Re: [DIYbio] Re: What is your biggest problem?

Yes, I agree. That is one aspect of many different issues of DNA-related work that are prohibitive to amateurs (others I've seen are sequencing and synthesis...)

On Wed, Mar 27, 2019 at 2:25 AM Koeng <koeng101@gmail.com> wrote:
My biggest problem is that the cost to clone plasmids is too damn high.

I'm not sure if there is a difference between wanting to change the world and having fun, for me it's sort of the same thing. By the time I'm done, the cost to clone plasmids (relative to now) will not be too damn high.

-Koeng

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Re: [DIYbio] Re: Engineering..a Sustainable Bioeconomy (Video, U.S. House Science, Space, and Technology Committee)

Oh yeah I miss not talking to the author of the green new deal. In one of the latest interviews she paints herself as the decider in charge. I have to redistrict myself. 

On Wednesday, March 27, 2019 at 1:28:16 AM UTC-4, Jonathan Cline wrote:
If you have something interesting to tell a congress person, you look
up their phone number by name in the directory and call them on the
phone.  That simple.   They will happily take opinions (at minimum,
their office person will).  I wouldnt want an office holder's time or
our citizen tax $$'s spent on reading or responding to ridiculous
trolling youtube comments anyway.  The lack might be google's fault
too- if google couldn't guarantee an accurate archivable digital
stream of all comments for government records then recording some
comments but not others would not be proper administration.

On 3/26/19, Yuriy <yuriy...@gmail.com> wrote:
> Funny how their youtube channel does not allow comments. Goes to show they
> know better and there is no room for difference of opinion.
>
> On Thursday, March 21, 2019 at 11:06:37 PM UTC-4, Jonathan Cline wrote:
>>
>> Below is the link to the excellent U.S. congressional hearing last month
>> on emerging biotech which fans of synbio should watch.  Starring Rob
>> Carlson.  Who incidentally, is the only one on either side of the table to
>>
>> quote real numbers  (plus, those numbers are amazing).
>>
>> Also a good reminder that the 2 'required reading' texts for synbio are,
>>
>> 1. Regenesis: How Synthetic Biology Will Reinvent Nature and Ourselves by
>>
>> George M. Church, ISBN 0465075703
>>
>> 2. Biology Is Technology: The Promise, Peril, and New Business of
>> Engineering Life by Robert H. Carlson,  ISBN 0674060156


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Re: [DIYbio] Re: Engineering..a Sustainable Bioeconomy (Video, U.S. House Science, Space, and Technology Committee)

That's an absolutist statement designed to discourage than to look for an unorthodox idea. 
They can afford to put an intern in charge of nonsense. That's what social media is for, Public Outreach and Relations.


On Tuesday, March 26, 2019 at 6:25:58 PM UTC-4, Cathal Garvey wrote:
To be fair, Youtube comments are a toilet of idiots, trolls, and angry teenagers. Nobody's got time for that nonsense.

On 26 March 2019 17:51:05 GMT+00:00, Yuriy <yuriy...@gmail.com> wrote:
Funny how their youtube channel does not allow comments. Goes to show they know better and there is no room for difference of opinion. 

On Thursday, March 21, 2019 at 11:06:37 PM UTC-4, Jonathan Cline wrote:
Below is the link to the excellent U.S. congressional hearing last month on emerging biotech which fans of synbio should watch.  Starring Rob Carlson.  Who incidentally, is the only one on either side of the table to quote real numbers  (plus, those numbers are amazing).

Also a good reminder that the 2 'required reading' texts for synbio are,

1. Regenesis: How Synthetic Biology Will Reinvent Nature and Ourselves by George M. Church, ISBN 0465075703

2. Biology Is Technology: The Promise, Peril, and New Business of Engineering Life by Robert H. Carlson,  ISBN 0674060156


Note I recommend watching at 2x speed or faster to make best use of time.  Recent misuse of genetic engineering is discussed throughout and iGEM is also mentioned.   "People doing bad things" is mentioned in vague, general, mostly idealistic terms.

HOWEVER: 

1. No mention of the massive fraud perpetrated by Theranos employees and exec's under the inflated umbrella hype created by MIT around synbio /nor/ any mention of the lack of scrutiny by government (even though Theranos's Board of Directors was populated with ex-government yes-men).  Hey government, where is the oversight?   Hey Rob Carlson, why not mention this massive government failure and lack of accountability in that same government? (As well as slip in the fact that those in congress are technologically out of their element and can't keep up because they don't have proper scientific staff or education themselves?)

2. No mention of the massive amount of intellectual property theft using "a hundred cut & pastes" regularly going on in emerging biotech, even though iGEM was mentioned (openwetware was not mentioned).   Hey government, where is the oversight?


--Citation--

Engineering Our Way to a Sustainable Bioeconomy https://www.youtube.com/watch?v=WYSfjDSxKK0
Date: Tuesday, March 12, 2019 - 10:00am
Location: 2318 Rayburn House Office Building
Subcommittees: 
Research and Technology (116th Congress)
Opening Statements
Chairwoman Haley Stevens (D-MI) of the Subcommittee on Research and Technology

Chairwoman Eddie Bernice Johnson (D-TX)

Witnesses
Dr. Rob Carlson, Managing Director of Bioeconomy Capital 

Dr. Kevin Solomon, Assistant Professor of Agricultural & Biological Engineering at Purdue University  

Dr. Eric Hegg, Professor of Biochemistry and Molecular Biology, Michigan State University; Michigan State University Subcontract Lead, Great Lakes Bioenergy Research Center  

Dr. Sean Simpson, Chief Scientific Officer and Co-Founder of LanzaTech 

Dr. Laurie Zoloth, Margaret E. Burton Professor of Religion and Ethics, and Senior Advisor to the Provost for Programs in Social Ethics at the University of Chicago 


House Science, Space, and Technology Committee
Streamed live on Feb 13, 2019
2318 Rayburn House Office Building
Washington, DC 20515


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