[DIYbio] Happy New Year!

I wish you all great success in your experiments, pursuits, and adventures! Lets make this one memorable and full of breakthroughs and most importantly have a ton of fun and learn something new! 

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

Very Urgent Requirement : Data Center Project Manager :: St. Paul, MN

 Hello,

 

Hope you doing well,

 

Please go through the requirement and let me know on dhruv@riderconsultinginc.com that you are comfortable.

 

Role : Data Center Project Manager

Location: St. Paul, MN

Duration: 1 year



Description:

Healthcare system has immediate openings for project manager to fill the role of Data Center Enhancement project manager. The project manager will lead the effort to determine current state and proposed state of the Fairview Data Center. This will include documentation of options/costs/etc., to present to senior leadership. Once path forward is determined (option chosen), the project manager will lead the implementation of the Data Center enhancement.

Skills:

·  Effectively establishes and maintains working relationships at all levels.

·  Excellent analytical and planning skills.

·  Demonstrated leadership.

·  Excellent communication and customer service skills.

·  Demonstrated flexibility and diplomacy.

·  Healthcare industry experience preferred.

·  Substantial 5-7 years Project Management experience.

 

 

Thanks,
Dhruv Soni

Phone : 218-656-0396
Email :
Dhruv@riderconsultinginc.com
Gtalk :
rider.dhruv1

 

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

Immediate Requirement : Front End Lead : NYC, NY

Hello,

 

Hope you doing well,

 

Please go through the requirement and let me know on dhruv@riderconsultinginc.com that you are comfortable.

 

Role : Front End Lead

Location : NYC,NY

Availability : Immediate

 

 

Job Description

 

Required Skills:

·         8+ years of industry experience, with a minimum of 3 years in user experience design

·         A deep understanding on UX design process and activities

·         Strong working knowledge on one/many RIA frameworks and JavaScript libraries (Angular JS, Bootstrap, Node.Js, Socket.io)

·         EXPERT in JavaScript,  HTML5 and CSS

·         Understanding of Java MVC frameworks (e.g. Spring MVC), RESTful Services will be a considered an advantage

·         Excellent communication and presentation skills

    • Ability to work independently and in a team 
    • Expertise with responsive web design 

 

 Thanks,
Dhruv Soni

Phone : 218-656-0396
Email :
Dhruv@riderconsultinginc.com
Gtalk :
rider.dhruv1

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

RE: Microfluidics Chat Thread WAS: [DIYbio] Paper request

Ah Sebastian, you rock. Thanks for the rigorous and methodical write-up. :)

On 31 December 2014 04:29:17 GMT+00:00, scocioba@gmail.com wrote:

See attached photo for an example of my crappy attempt at making pdms more hydrophilic using a UV sterilizer (the breadbox type). 30 minute exposure on both a piranha-treated microscope slide (15min in a solution of 3:1 h2so4:h2o2 *dangerous, corrosive, always use appropriate PPE*) and a freshly minted pdms chip. I dried the slide with a kimwipe and used scotch tape to remove residual dust from the feature side of the pdms chip. Then I just laid the slide and pdms chip (feature side up) into the chamber and set a timer for 30 minutes. Then quickly place the pdms chip feature side down onto the slide and apply gentle pressure around the features. Be sure to not squash the channels.

PDMS was the knock-off ML Solar brand from eBay. 10:1 mix by weight. Degassed in a desiccation chamber to 25+inHg twice and incubated at 60c for 1hr.

Pdms chip was casted in a plastic Petri dish on a shrunk shrinky dink printed using graffix brand shrinkable sheet (white not clear!). Something funky about the clear version. Sticks to everything when in the oven, got better results with white sheets. Printed with a standard brother monochrome laser printer (toner). Chip design taken from template PowerPoint slide from this site:

http://chemistry.beloit.edu/Edetc/nanolab/shrink/index.html

Its a simple gradient generator. Still not as fluid and wettable as I'd like it to be. I used a straw to mouth-vacuum from the outlet port. Aside from some funky phenomena on the rightmost channel, it did seem to separate decently. I used food coloring as the indicator.

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D

From: Nathan McCorkle
Sent: ‎12/‎29/‎2014 3:21 AM
To: diybio
Subject: Re: Microfluidics Chat Thread WAS: [DIYbio] Paper request

On Sun, Dec 28, 2014 at 8:47 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
> On Sun, Dec 28, 2014 at 8:45 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
>> https://raw.githubusercontent.com/nmz787/microfluidic-cad/master/implicitCAD/output/tobacco_mesophyll_protoplast_fusion_device.jpg
>
> and yeah I know I need to improve the funnel-of-posts angle so the
> edges follow the pitch of the posts, so i.e. cells don't get stuck.

Which is demonstrated on the macroscale with marbles:
https://www.youtube.com/watch?v=03tx4v0i7MA



--
Sent from my Android device with K-9 Mail. Please excuse my brevity.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

Re: [DIYbio] Digest for diybio@googlegroups.com - 10 updates in 3 topics

Haha! No methane please!

On Tue, Dec 30, 2014 at 9:19 PM, <diybio@googlegroups.com> wrote:
<scocioba@gmail.com>: Dec 30 11:29PM -0500

See attached photo for an example of my crappy attempt at making pdms more hydrophilic using a UV sterilizer (the breadbox type). 30 minute exposure on both a piranha-treated microscope slide (15min in a solution of 3:1 h2so4:h2o2 *dangerous, corrosive, always use appropriate PPE*) and a freshly minted pdms chip. I dried the slide with a kimwipe and used scotch tape to remove residual dust from the feature side of the pdms chip. Then I just laid the slide and pdms chip (feature side up) into the chamber and set a timer for 30 minutes. Then quickly place the pdms chip feature side down onto the slide and apply gentle pressure around the features. Be sure to not squash the channels.
 
PDMS was the knock-off ML Solar brand from eBay. 10:1 mix by weight. Degassed in a desiccation chamber to 25+inHg twice and incubated at 60c for 1hr.
 
Pdms chip was casted in a plastic Petri dish on a shrunk shrinky dink printed using graffix brand shrinkable sheet (white not clear!). Something funky about the clear version. Sticks to everything when in the oven, got better results with white sheets. Printed with a standard brother monochrome laser printer (toner). Chip design taken from template PowerPoint slide from this site:
 
http://chemistry.beloit.edu/Edetc/nanolab/shrink/index.html
 
Its a simple gradient generator. Still not as fluid and wettable as I'd like it to be. I used a straw to mouth-vacuum from the outlet port. Aside from some funky phenomena on the rightmost channel, it did seem to separate decently. I used food coloring as the indicator.
 
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D
 
-----Original Message-----
From: "Nathan McCorkle" <nmz787@gmail.com>
Sent: ‎12/‎29/‎2014 3:21 AM
To: "diybio" <diybio@googlegroups.com>
Subject: Re: Microfluidics Chat Thread WAS: [DIYbio] Paper request
 
>> https://raw.githubusercontent.com/nmz787/microfluidic-cad/master/implicitCAD/output/tobacco_mesophyll_protoplast_fusion_device.jpg
 
> and yeah I know I need to improve the funnel-of-posts angle so the
> edges follow the pitch of the posts, so i.e. cells don't get stuck.
 
Which is demonstrated on the macroscale with marbles:
https://www.youtube.com/watch?v=03tx4v0i7MA
 
 
 
--
-Nathan
 
--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CA%2B82U9KjSjhbOHkVr-cv3S-TnnNfP8mzvM19q%2BuARWXKEdKhtw%40mail.gmail.com.
For more options, visit https://groups.google.com/d/optout.
<scocioba@gmail.com>: Dec 30 02:08PM -0500

I'm lounging around at the museum of natural history and during some downtime I stumbled upon yet another paywalled article about plant protoplasts and microfluidics:
 
Developing microfluidics for rapid protoplasts collection and lysis from plant leaf
 
http://pin.sagepub.com/content/226/1/15.abstract
 
This one is interesting since it works with isolation, single cell sieving, and lysis for DNA extraction all in one chip. Would anyone care to help a broke plant biologist this holiday season? Thanks in advance!
 
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D
Tom Knight <tk@mit.edu>: Dec 30 02:14PM -0500

<scocioba@gmail.com>: Dec 30 02:15PM -0500

Great idea. Sure beats spamming the list for my needs.
 
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D
 
-----Original Message-----
From: "Tom Knight" <tk@mit.edu>
Sent: ‎12/‎30/‎2014 2:14 PM
To: "diybio@googlegroups.com" <diybio@googlegroups.com>
Cc: "Thomas Knight" <tk@mit.edu>
Subject: Re: [DIYbio] Yet another paper request
 
Here you are.
If you want a more predictable set of replies, then I'd recommend the Bioforum group at protocol-online.org. The "Papers" subgroup will find virtually anything, and has people with access to things I can't get. The group is friendly in general.
 
--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/5E30027A-8E1A-4C99-B20A-13103B6080BA%40mit.edu.
For more options, visit https://groups.google.com/d/optout.
Tom Hodder <tom@limepepper.co.uk>: Dec 30 07:34PM

try these 2
http://sci-hub.org/
http://gen.lib.rus.ec/
 
they are obviously as dodgy as a 3 pound note, but they usually have what
you want and can also be configured to transparently proxy most of the
journals web sites.
 
Tom Hodder <tom@limepepper.co.uk>: Dec 30 07:35PM

oops. that was meant to be off list. Sorry.
 
Nathan McCorkle <nmz787@gmail.com>: Dec 30 05:56PM -0800

> Developing microfluidics for rapid protoplasts collection and lysis from
> plant leaf
 
> http://pin.sagepub.com/content/226/1/15.abstract
 
This is another really cool design. Lots of great ideas!
Sebastian Cocioba <scocioba@gmail.com>: Dec 30 09:25PM -0500

Yeah the clever cell sorting array is what I'm super curious about. I want
to see if my toner printer can handle such small structures using shrinky
dinks. Just going to overlay my hemocytometer on the chip and make various
posts in a PDMS chip and see what works. Know of any high DPI laser
printers for cheap?
 
mostromundo <mzuorski@gmail.com>: Dec 29 09:50PM -0800

I work in wine production, we want yeast that will consume all of the
hexose sugars (especially fructose) and will do so while producing more
glycerol and less ethanol. If you can make a fructophilic wine yeast with
below-average ethanol production and acceptable sensory characters, you
will make a lot of winemakers very happy :)
 
On Friday, December 26, 2014 9:29:46 AM UTC-8, Nick M. wrote:
"Mega [Andreas Stuermer]" <masterstorm123@gmail.com>: Dec 30 12:17AM -0800

That actually seems doable, but the yeast would grow slower. All the enzymes have been known for a long time. Unless you add methane production for energy making
You received this digest because you're subscribed to updates for this group. You can change your settings on the group membership page.
To unsubscribe from this group and stop receiving emails from it send an email to diybio+unsubscribe@googlegroups.com.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CAJLS%2BSBeGgKGQFp0PHTfJ1m6_-39X7Whon4rZezAf5ckhDrrpw%40mail.gmail.com.
For more options, visit https://groups.google.com/d/optout.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

RE: Microfluidics Chat Thread WAS: [DIYbio] Paper request


See attached photo for an example of my crappy attempt at making pdms more hydrophilic using a UV sterilizer (the breadbox type). 30 minute exposure on both a piranha-treated microscope slide (15min in a solution of 3:1 h2so4:h2o2 *dangerous, corrosive, always use appropriate PPE*) and a freshly minted pdms chip. I dried the slide with a kimwipe and used scotch tape to remove residual dust from the feature side of the pdms chip. Then I just laid the slide and pdms chip (feature side up) into the chamber and set a timer for 30 minutes. Then quickly place the pdms chip feature side down onto the slide and apply gentle pressure around the features. Be sure to not squash the channels.

PDMS was the knock-off ML Solar brand from eBay. 10:1 mix by weight. Degassed in a desiccation chamber to 25+inHg twice and incubated at 60c for 1hr.

Pdms chip was casted in a plastic Petri dish on a shrunk shrinky dink printed using graffix brand shrinkable sheet (white not clear!). Something funky about the clear version. Sticks to everything when in the oven, got better results with white sheets. Printed with a standard brother monochrome laser printer (toner). Chip design taken from template PowerPoint slide from this site:

http://chemistry.beloit.edu/Edetc/nanolab/shrink/index.html

Its a simple gradient generator. Still not as fluid and wettable as I'd like it to be. I used a straw to mouth-vacuum from the outlet port. Aside from some funky phenomena on the rightmost channel, it did seem to separate decently. I used food coloring as the indicator.

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D

From: Nathan McCorkle
Sent: ‎12/‎29/‎2014 3:21 AM
To: diybio
Subject: Re: Microfluidics Chat Thread WAS: [DIYbio] Paper request

On Sun, Dec 28, 2014 at 8:47 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
> On Sun, Dec 28, 2014 at 8:45 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
>> https://raw.githubusercontent.com/nmz787/microfluidic-cad/master/implicitCAD/output/tobacco_mesophyll_protoplast_fusion_device.jpg
>
> and yeah I know I need to improve the funnel-of-posts angle so the
> edges follow the pitch of the posts, so i.e. cells don't get stuck.

Which is demonstrated on the macroscale with marbles:
https://www.youtube.com/watch?v=03tx4v0i7MA



--
-Nathan

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CA%2B82U9KjSjhbOHkVr-cv3S-TnnNfP8mzvM19q%2BuARWXKEdKhtw%40mail.gmail.com.
For more options, visit https://groups.google.com/d/optout.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

Re: [DIYbio] Yet another paper request

Yeah the clever cell sorting array is what I'm super curious about. I want to see if my toner printer can handle such small structures using shrinky dinks. Just going to overlay my hemocytometer on the chip and make various posts in a PDMS chip and see what works. Know of any high DPI laser printers for cheap?

On Tue, Dec 30, 2014 at 8:56 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
On Tue, Dec 30, 2014 at 11:08 AM,  <scocioba@gmail.com> wrote:
> Developing microfluidics for rapid protoplasts collection and lysis from
> plant leaf
>
> http://pin.sagepub.com/content/226/1/15.abstract

This is another really cool design. Lots of great ideas!

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CA%2B82U9%2BA8THKs8n5sDsg%3Dn-8uL4BpW%3Dkqu9KWB9_ipyLQWPQPA%40mail.gmail.com.
For more options, visit https://groups.google.com/d/optout.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CANH1KJJv4Rvn04eh4xp26SrTN_UMqjA9f-3gkh_WKajEjwAhsA%40mail.gmail.com.
For more options, visit https://groups.google.com/d/optout.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

Re: [DIYbio] Yet another paper request

On Tue, Dec 30, 2014 at 11:08 AM, <scocioba@gmail.com> wrote:
> Developing microfluidics for rapid protoplasts collection and lysis from
> plant leaf
>
> http://pin.sagepub.com/content/226/1/15.abstract

This is another really cool design. Lots of great ideas!

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CA%2B82U9%2BA8THKs8n5sDsg%3Dn-8uL4BpW%3Dkqu9KWB9_ipyLQWPQPA%40mail.gmail.com.
For more options, visit https://groups.google.com/d/optout.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

Re: [DIYbio] Yet another paper request

oops. that was meant to be off list. Sorry.

On 30 December 2014 at 19:34, Tom Hodder <tom@limepepper.co.uk> wrote:

they are obviously as dodgy as a 3 pound note, but they usually have what you want and can also be configured to transparently proxy most of the journals web sites.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CAB%2BzPJAgrR%3De1kOWXSk2Wb5_d8da1bVfnaXCmY6bdxrcrBDf8g%40mail.gmail.com.
For more options, visit https://groups.google.com/d/optout.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

Re: [DIYbio] Yet another paper request

try these 2
http://gen.lib.rus.ec/

they are obviously as dodgy as a 3 pound note, but they usually have what you want and can also be configured to transparently proxy most of the journals web sites.

On 30 December 2014 at 19:15, <scocioba@gmail.com> wrote:
Great idea. Sure beats spamming the list for my needs.

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D

From: Tom Knight
Sent: ‎12/‎30/‎2014 2:14 PM
To: diybio@googlegroups.com
Cc: Thomas Knight
Subject: Re: [DIYbio] Yet another paper request

Here you are.
If you want a more predictable set of replies, then I'd recommend the Bioforum group at protocol-online.org. The "Papers" subgroup will find virtually anything, and has people with access to things I can't get. The group is friendly in general.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/5E30027A-8E1A-4C99-B20A-13103B6080BA%40mit.edu.
For more options, visit https://groups.google.com/d/optout.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/54a2f9f4.e1608c0a.4fee.0cbc%40mx.google.com.

For more options, visit https://groups.google.com/d/optout.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CAB%2BzPJC2%2BGMg1JLT1L46uHPurVw0fYTsyepAd%3D-DOMiTRPnGvA%40mail.gmail.com.
For more options, visit https://groups.google.com/d/optout.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

RE: [DIYbio] Yet another paper request

Great idea. Sure beats spamming the list for my needs.

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D

From: Tom Knight
Sent: ‎12/‎30/‎2014 2:14 PM
To: diybio@googlegroups.com
Cc: Thomas Knight
Subject: Re: [DIYbio] Yet another paper request

Here you are.
If you want a more predictable set of replies, then I'd recommend the Bioforum group at protocol-online.org. The "Papers" subgroup will find virtually anything, and has people with access to things I can't get. The group is friendly in general.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/5E30027A-8E1A-4C99-B20A-13103B6080BA%40mit.edu.
For more options, visit https://groups.google.com/d/optout.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

Re: [DIYbio] Yet another paper request

Here you are.
If you want a more predictable set of replies, then I'd recommend the Bioforum group at protocol-online.org. The "Papers" subgroup will find virtually anything, and has people with access to things I can't get. The group is friendly in general.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/5E30027A-8E1A-4C99-B20A-13103B6080BA%40mit.edu.
For more options, visit https://groups.google.com/d/optout.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

[DIYbio] Yet another paper request

I'm lounging around at the museum of natural history and during some downtime I stumbled upon yet another paywalled article about plant protoplasts and microfluidics:

Developing microfluidics for rapid protoplasts collection and lysis from plant leaf

http://pin.sagepub.com/content/226/1/15.abstract

This one is interesting since it works with isolation, single cell sieving, and lysis for DNA extraction all in one chip. Would anyone care to help a broke plant biologist this holiday season? Thanks in advance!

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

[DIYbio] Re: saccharomyces cerevisiae potentialities

That actually seems doable, but the yeast would grow slower. All the enzymes have been known for a long time. Unless you add methane production for energy making

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/6435776f-d47a-4411-8758-b59202c1c93b%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

[DIYbio] Re: saccharomyces cerevisiae potentialities

I work in wine production, we want yeast that will consume all of the hexose sugars (especially fructose) and will do so while producing more glycerol and less ethanol. If you can make  a fructophilic wine yeast with below-average ethanol production and acceptable sensory characters, you will make a lot of winemakers very happy :)

On Friday, December 26, 2014 9:29:46 AM UTC-8, Nick M. wrote:

All, 

Hope everyone had lovely and peaceful holidays. 

I've been lurking the group for ~16 months now. So far I've done a GFP plant, selective breeding on plants, selective breeding on brewer's yeast, and a few other random projects. I want to thank you all for the invaluable depth of knowledge that is available here.

I am a brewer by profession and have recently taken over a brewery with considerable corporate backing, corporate has told me 'Do what you want, don't break anything, and most importantly make great beer.' 

I take this to me I can build my own synbio lab, I am confident that is within my AOR. 

 I was hoping to receive some input from you all on interesting directions to go with S. cerevisiae. 

I have seen the iGEM projects on beer, caffeine, limonene(particularly interesting to me), and kumamolisin, and all of those are things I would like to work towards. 

Any ideas on what I can do in the interim whilst trial running my lab and knowledge base? Looking for projects that would be valuable to me in either an academic or practical regard that would build a good framework of experience to operate on in a professional standard. I also feel indebted to the community and must offer my position to the benefit to the diybio community once it becomes a position of value. 

Cheers,
Nick 

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/1a0f8241-1a42-4a75-a863-b48f7cdfc84a%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

[DIYbio] shRNA construct template

Hi everyone. 
Just wondering if anyone could share an .ape file or .dna file of a well annotated silencer construct. 

In papers they always give the sequence of the actual silencing part, but never the entire sequence like 


ATATTTTATATATATAgagtctgcgctagtATATATATATATAAAATATAAAAAA

hairpin-forming sequence
silencing RNA part (complementary to target gene)
vector backbone

The silencing constructs I designed so far all rely on a tutorial, but I'd rather have one sequence that was shown to work and just replace the silencing part 
 e.g. 
ATATTTTATATATATAnnnnnnnnnnATATATATATATAAAATATAAAAAA

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/61cb42dc-c473-495f-aacc-ceb60d9bce1b%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

RE: Microfluidics Chat Thread WAS: [DIYbio] Paper request

At the moment, my main strife is with my vacuum pump. Its a single stage that can kinda almost not really go to 29"Hg. The MIT nanomaker video on pdms oxidation via microwave oxygen plasma said we need to pull a vacuum of 6 torr or less (<29.8inHg) I don't really trust my vacuum gauge but after some fitting tightening and Teflon taping, I got it to go to 29"Hg. Most two stage pumps state they can down to 6 torr but I really don't feel like buying another pump albeit for the low price of $114.

I went to Brooklyn Glass a while back and the neon guy showed me air plasma in a neon tube but I forgot what pressure he pulled. The experiment here:

https://m.youtube.com/watch?v=fMUemBZ0k5Q

Is so simple but a few setbacks have made me a bit frustrated. The two all-plastic vales I installed into my Pyrex bottle cap had some fun quirks. That ended up causing a leak. I fixed that problem with some JB Weld but now it seems my vacuum pump can't pull low enough. I found a corona treater on eBay for cheap but only has the needle probe not the wide angle spreader. Worst case ill try that. Also found an interesting article on UV based pdms oxidation via ozone but its paywalled for me:

http://www.sciencedirect.com/science/article/pii/S0925400503007482

I'm getting the hang of draftsight but I don't trust the hatching it does to fill in the spaces to make a printable negative. It does a "low-poly" type fill that leaves these secant gaps around curves which may interfere with the channels. I guess I could just reflow the toner ink. I'm using shrinkydinks at the moment....

Why is it so complex to make simple shapes using CAD programs?!?! I actually found LibreCAD to be quicker to use. Seems like it has the same functionality and works fine for building fluidics. Just make sure to use closed polylines, not lines, and join them as you go. Kinda like compiling code often to avoid bugs later on.

In DraftSight, once u lay a polyline, just right click and hit arc to make curves. I always snap to grid and Ortho since my first circuits are just gradient generators and are more or less square with the grid. If the arc doesn't form in the direction you want, just right click and hit direction and draw a line in the direction you want and that should fix the arc issue. Once your chip circuit is done, highlight all the points and right click, editPolyline, type join, hit enter. That should make everything one big polygon if all is closed.

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D

From: Nathan McCorkle
Sent: ‎12/‎29/‎2014 3:21 AM
To: diybio
Subject: Re: Microfluidics Chat Thread WAS: [DIYbio] Paper request

On Sun, Dec 28, 2014 at 8:47 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
> On Sun, Dec 28, 2014 at 8:45 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
>> https://raw.githubusercontent.com/nmz787/microfluidic-cad/master/implicitCAD/output/tobacco_mesophyll_protoplast_fusion_device.jpg
>
> and yeah I know I need to improve the funnel-of-posts angle so the
> edges follow the pitch of the posts, so i.e. cells don't get stuck.

Which is demonstrated on the macroscale with marbles:
https://www.youtube.com/watch?v=03tx4v0i7MA



--
-Nathan

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CA%2B82U9KjSjhbOHkVr-cv3S-TnnNfP8mzvM19q%2BuARWXKEdKhtw%40mail.gmail.com.
For more options, visit https://groups.google.com/d/optout.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

[DIYbio] Re: saccharomyces cerevisiae potentialities

Ah, I think I came across a glucanase which destroys gram+ bacteria in literature research in my bee project. I didn't look closer at it, because bacteria seem to be much much  lesser a problem to bees than fungi and viruses and pesticides. 

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/57a6fdc4-a3c6-4821-afb7-90f511d52e44%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

[DIYbio] Re: saccharomyces cerevisiae potentialities

Make the yeast produce nisin, so you get ride of lactobacilli which may destroy beer quality? Or some vinegar bacterium-antimicrobial peptide?

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/a3740fbe-d88d-4ceb-9e17-fbc1ec0a59a6%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

Re: [DIYbio] Any DIYBio/Biohacking Youtube Channels or Anything

Is there any channels that are active though?

On Monday, December 29, 2014 2:45:08 AM UTC-5, Tom Hodder wrote:
On 28 December 2014 at 19:37, Rome Robinson <rxxpr...@gmail.com> wrote:

Are there any DIYBio/Biohacking Youtube Channels? The reason I want to know is because I would like to see what other people in the DIYBio community are doing and also gain information and knowledge.

The London Biohackers have a channel but its a bit neglected atm. 

There are a few things from a while ago;


 

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/22f472c5-da7a-4ca3-8442-c6a4d62afee3%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS