Hi everyone.
-- Just wondering if anyone could share an .ape file or .dna file of a well annotated silencer construct.
In papers they always give the sequence of the actual silencing part, but never the entire sequence like
ATATTTTATATATATAgagtctgcgctagtATATATATATATAAAATATAAAAAA
hairpin-forming sequence
silencing RNA part (complementary to target gene)
vector backbone
The silencing constructs I designed so far all rely on a tutorial, but I'd rather have one sequence that was shown to work and just replace the silencing part
e.g.
ATATTTTATATATATAnnnnnnnnnnATATATATATATAAAATATAAAAAA
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