Looking Genesys Developer, CT

Hi ,

 

Hope you are doing good, and we got your profile from monster for below position. If you are available to take new projects, please reach me at praveenk@xperttech.com  / 781-780-2939*3005

 

Job Title: Genesys Developer

Location: Hartford, CT

Duration: 6+ Months

Billing Rate: $DOE/Hour

 

 

EXPERIENCE SKILL MATRIX:

Total years of Experience in IT: years

Total years of US Experience: years

 

1. COMPLETE SKILL MATRIX (YEARS OF EXPERIENCE IN EACH SKILL):

1.       Genesys Developer: years

2.       Application Operation, Maintenance and Development: years

3.       Genesys Voice Portal (GVP) and Framework: years

4.       Genesys Inbound Voice Application 8.1, GVP,WFM, Infomart, Interaction Workspace: years

 

Job Description:

1.       Genesys Framework, Genesys Voice Platform(GVP) including SIP Server, URS, Config Server, DB Server, Routing Stat Servers, Reporting Stat Servers, Interaction Center

2.       Application Operation, Maintenance and Development

3.       Genesys Voice Portal (GVP) and Framework

4.       Genesys Inbound Voice Application 8.1, GVP,WFM, Infomart, Interaction Workspace

5.       Change, Incident/Service Request/ Release Management on Genesys, Upgrades of Genesys components

 

 

Thanks/Regards,

 

Praveen Kumar

 

400 W Cummings Park

Suite#2850

Woburn, MA-01801

Email: praveenk@xperttech.com

Phone: 781-780-2939 Ext 3005

Fax: 978-405-5040

www.xperttech.com

 

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Fwd: Urgent Client Need; Sr Java Developer; 6+Months Contract; Denver, CO (Cable Industry Experience is Must)

Please send your resumes at niranjan@niranjan.kumar@vision3solutions.com

Hi

 

This is Niranjan from Vision3solutions; hope you are doing well.

Please go through below description and reply with your resume, contact details and current location, if you feel comfortable

 

 

Job role: Sr Java Developer

Duration: 6+Months Contract

Location: Denver, CO

 

Cable Industry Experience is Must

 

Description

Consultant position in the Services group at Client  plays an active role in planning and implementing a web service infrastructure to support next generation products. Client is making a transition to Internet delivered video and content featuring cloud based services and highly interactive HTML5 and native UI's across a variety of consumer electronics devices and set top boxes.

Education/Experience

 

•       7+ years of experience demonstrating an in-depth capability as a software engineer

 

•       B.S. in Computer Science or related field

 

Qualifications

 

·         Demonstrate a thorough understanding of software engineering and object oriented design principles

·         Strong and current development experience in Java and web technologies

·         Ability to take ownership and make technical decisions on software development projects

·         Implement software proposals, estimate scope of work, create project documentation

·         Familiarity with dependency management and build systems like Maven and Ant.

·         Experience working with open source software such as Spring, Google Guice, Guava, Tomcat, and NoSQL solutions

 

•       Hands on approach a must

 

·         Agile experience a plus

·         Experience with AWS, micro services, and Netflix OSS tools is a plus

 

Technical Skill-set

Java, Spring, Google Guice, JPA/Hibernate, Databases (Relational and NoSQL), RESTful web services, AWS, Micro services, Netflix OSS, Jersey, SOAP web services, Axis, JMS, ESB's, XML, XSD, XSL, JSON, HTML5, JavaScript, Apache open source libraries, Maven, Git, Eclipse/NetBeans/IntelliJ, continuous integration testing, shell scripting, Ruby

 

Thanks& Regards

 

Niranjan Kumar

 

 

Vision3 Solutions Inc

8155 Cavendish Place,Suwanee, GA 30024
Contact No:
(678) 554-4785
Email: niranjan.kumar@vision3solutions.com

                 niruk5503@gmail.com


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Urgent Client Need; Sr Java Developer; 6+Months Contract; Denver, CO (Cable Industry Experience is Must)

Please send your resumes at niranjan@niranjan.kumar@vision3solutions.com

Hi

 

This is Niranjan from Vision3solutions; hope you are doing well.

Please go through below description and reply with your resume, contact details and current location, if you feel comfortable

 

 

Job role: Sr Java Developer

Duration: 6+Months Contract

Location: Denver, CO

 

Cable Industry Experience is Must

 

Description

Consultant position in the Services group at Client  plays an active role in planning and implementing a web service infrastructure to support next generation products. Client is making a transition to Internet delivered video and content featuring cloud based services and highly interactive HTML5 and native UI's across a variety of consumer electronics devices and set top boxes.

Education/Experience

 

•       7+ years of experience demonstrating an in-depth capability as a software engineer

 

•       B.S. in Computer Science or related field

 

Qualifications

 

·         Demonstrate a thorough understanding of software engineering and object oriented design principles

·         Strong and current development experience in Java and web technologies

·         Ability to take ownership and make technical decisions on software development projects

·         Implement software proposals, estimate scope of work, create project documentation

·         Familiarity with dependency management and build systems like Maven and Ant.

·         Experience working with open source software such as Spring, Google Guice, Guava, Tomcat, and NoSQL solutions

 

•       Hands on approach a must

 

·         Agile experience a plus

·         Experience with AWS, micro services, and Netflix OSS tools is a plus

 

Technical Skill-set

Java, Spring, Google Guice, JPA/Hibernate, Databases (Relational and NoSQL), RESTful web services, AWS, Micro services, Netflix OSS, Jersey, SOAP web services, Axis, JMS, ESB's, XML, XSD, XSL, JSON, HTML5, JavaScript, Apache open source libraries, Maven, Git, Eclipse/NetBeans/IntelliJ, continuous integration testing, shell scripting, Ruby

 

Thanks& Regards

 

Niranjan Kumar

 

 

Vision3 Solutions Inc

8155 Cavendish Place,Suwanee, GA 30024
Contact No:
(678) 554-4785
Email: niranjan.kumar@vision3solutions.com

                 niruk5503@gmail.com

 

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[DIYbio] Re: DNA synthesis at 10 cents per letter

Haven't heard of any DIY systems for DNA synthesis yet but I think DNA separation by silica adsorption is part of the method. It might be worth looking into for anyone interested in making such a system. I'm sure both community and industry labs would be very interested in something like that!

On Saturday, November 21, 2015 at 3:27:51 PM UTC-8, technologiclee wrote:
http://www.wired.com/2015/11/making-dna/
"When Twist launches its beta program in 2016, it will offer gene synthesis at 10 cents per letter with a guaranteed turnaround time of 10 days."

"Moreover, the silicon wafer is cleverly optimized for the second step of gene synthesis—the stitching of oligos together—because Twist's engineers figured out how to cut down on moving tiny volumes of liquid. Twist's proprietary machine, a small-car sized system that WIRED wasn't allowed to photograph, deposits one oligo into each of the 100 or so holes inside of a nanowell."

Is there any DIY system capable of this? What would it take to do that? What is the basic mechanism of moving the fluid in this system?

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Re: [DIYbio] Re: Book or Blog?

I'd say Simon has the most experience with this stuff and he has good advice.

Still, I always prefer a nice tangible hardcover or softcover book.  I stare at my screen long enough all day, and I have thousands of bookmarks I'll never go back to and forget about.  I love being able to pick up a book and turn to the page I need. 

On Nov 29, 2015 8:07 AM, "Jérôme Lutz" <jerome@synbio.info> wrote:
Hi Sebastian, Hi everyone, 

what about a wiki? 

I asked myself the same question back in April and decided to put everything I learn about SynBio on my now open and non-profit wiki at www.synbio.info

The reasons for this were quite simple: 
  • In a blog, everything is structured according to the day you write the blog post. How should one find what he or she is looking for? In a wiki, you have to think about a way how to structure your knowledge so you can easily retrieve it. 
  • I am rather new to the field and don't have a biology background, and of course - you can never know everything. Meanwhile we are 33 users, out of which 5-10 are pretty active contributing their own field's of experience. They just start writing and then we talk together on Slack or within the wiki on how to improve the articles. Once we are happy, we share it to our 10.000+ Facebook fans at https://www.facebook.com/synbioinfo
  • In a book, you spend years of researching things and then you publish - but our field is advancing way too fast and stuff you write today and publish say in 2 years is often outdated already. Also as a ready, you don't have the chance to dig deeper into a topic when you are interested. In the wiki, we try to put as many links to a topic as relevant and people keep adding they find to the wiki page when they dig deeper. 
  • However, a great book I currently read is BioBuilder. I like this as a book, because it gives me all information from A-Z. But well, I read it and then I put it on the book shelf. On the wiki, I am working daily and use it as a "outsourced" brain memory, so when I forget a detail I find it with the search within seconds and that's much faster then skipping through the index of a book and rereading whole passages.  
So a wiki was for me the best choice. Also, I have been setting up knowledge management systems for pretty much all the companies I worked in so choosing Confluence from Atlassian was clear for me as well. The cool thing about this proprietary software is that it's from a pretty cool company and they offer free licenses for the software if you are a non-profit. Since I am a big fan of open knowledge, that just made sense as well. 

But how do we make money? 
Well, we found a very interesting business model for the non-profit: We are mostly software developers and we have to adapt the standard Confluence quite a bit to make this wiki happen the way we want it. So far, we have developed 45+ macros, little pieces of software that e.g. build a glossary for biological terms or manage the content creation process. We will soon start selling those on the Confluence Marketplace, and by this we can finance the servers, do some marketing and maybe even pay authors one day (however, not sure if that's the point of an open wiki.. what do you guys think? I am still uncertain about that point).

If you are interested, I would be happy to show you around the Wiki and we can see if we do something together! Basically, we could either set up a "menu point" on synbio.info for plant biology or even start an own wiki, as we recently did for bio art at http://art.synbio.info. What do you think? 

Talk to you soon and best regards from way too cold Munich, 

Jérôme



Am Samstag, 28. November 2015 19:21:57 UTC+1 schrieb Sebastian:
Hi Everyone,
I've been bouncing around the idea of compiling the 7+ years of plant tissue culture experience I've mustered into articles for my blog for a long time now. I have a ton of content as drafts and galleries of original pictures and whatnot and am stuck at a crossroads and would like some advice. My plan was to keep posting small articles to my blog about the little steps needed to make a transgenic plant in very newbie-friendly detail. A lot of my contacts recommended I just compile a book and publish it so that I can get a little compensation for the information that would be a little hard to come by in that specific format. I have always put teaching people first and tend to forget about supporting myself which is what led me to this intersection. 

Would people rather have a nice free blog that steadily outputs content with some tasteful adds pertaining to relevant companies and products, or pay a small price for a Julia Child-style cookbook of plant tissue culture and transformation recipes concocted by yours truly with plenty of pictures and commentary?

 What would be a good price point?

 Is it worth the delay to have a physical edition one can tote to their bench or is it more useful to have an organized blog website thingy that people can reference digitally?

What would be a good starting project? I was thinking a GUS assay since it covers a bunch of various topics and the plasmid is commercially available and off-patent. I'm not a fan of GFP since the stable ones require harmful UV and I have yet to transform the couple fluorescent plasmids I've optimized into tobacco to verify that long wave UV will work with decent effect. Another option would be anthocyanin knockouts/add-ins or variegation gene introduction but would require further testing. GUS requires a bit more chemicals but the result is a strong insoluble blue precipitate against a white background of ethanol-bleached plants. No fancy equipment necessary! I'm also not stepping foot anywhere near glowing plants for many reasons so please don't ask to make a book about making a glowing plant from scratch. That being said, if you had a plant biotech cookbook, what would be your desired "Hello World" project? Herbicide tolerance alone is boring, non-visual, and controversial/unethical in spread so that's out of the question. It's kinda funny that the perfect intro project would require a lot of research on my part into verifying chromoprotein stability in lower pH (plant cytosol is ~5.6 - 5.8) and shipping things to the vacuole, etc, etc. so any feedback on what you all want to have as the main project of the book (if you even want a book in the first place) would be awesome!

I thought I would ask the community that would be most interested in learning more about the dark-green arts (you gals and guys) if it would even be worth the trouble. I've noticed an overwhelming lack of good plant biotech material out there and there are too many comments about plant culturing being "hard to do" and "unpredictable" which I think is just silly. Anyone can do science and everyone can do plant biotech. The hardest part seems to be aseptic technique and culture-to-culture media decisions but those are the most straight forward. I am a very visual learner so I would include a ton of pictures with various angles and explanations so anyone ages ~8 and up (maybe even earlier if fine motor skills are honed!) can make their own plants. Either way let me know what y'all think about the idea of Book vs Blog for introductory plant tissue culture. I would still maintain my blog for the day to day content and more advanced topics but for the newbie I think a desk reference at a fair price would be ideal. Thanks!

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[DIYbio] Re: Book or Blog?

Hi Sebastian, Hi everyone, 

what about a wiki? 

I asked myself the same question back in April and decided to put everything I learn about SynBio on my now open and non-profit wiki at www.synbio.info

The reasons for this were quite simple: 
  • In a blog, everything is structured according to the day you write the blog post. How should one find what he or she is looking for? In a wiki, you have to think about a way how to structure your knowledge so you can easily retrieve it. 
  • I am rather new to the field and don't have a biology background, and of course - you can never know everything. Meanwhile we are 33 users, out of which 5-10 are pretty active contributing their own field's of experience. They just start writing and then we talk together on Slack or within the wiki on how to improve the articles. Once we are happy, we share it to our 10.000+ Facebook fans at https://www.facebook.com/synbioinfo
  • In a book, you spend years of researching things and then you publish - but our field is advancing way too fast and stuff you write today and publish say in 2 years is often outdated already. Also as a ready, you don't have the chance to dig deeper into a topic when you are interested. In the wiki, we try to put as many links to a topic as relevant and people keep adding they find to the wiki page when they dig deeper. 
  • However, a great book I currently read is BioBuilder. I like this as a book, because it gives me all information from A-Z. But well, I read it and then I put it on the book shelf. On the wiki, I am working daily and use it as a "outsourced" brain memory, so when I forget a detail I find it with the search within seconds and that's much faster then skipping through the index of a book and rereading whole passages.  
So a wiki was for me the best choice. Also, I have been setting up knowledge management systems for pretty much all the companies I worked in so choosing Confluence from Atlassian was clear for me as well. The cool thing about this proprietary software is that it's from a pretty cool company and they offer free licenses for the software if you are a non-profit. Since I am a big fan of open knowledge, that just made sense as well. 

But how do we make money? 
Well, we found a very interesting business model for the non-profit: We are mostly software developers and we have to adapt the standard Confluence quite a bit to make this wiki happen the way we want it. So far, we have developed 45+ macros, little pieces of software that e.g. build a glossary for biological terms or manage the content creation process. We will soon start selling those on the Confluence Marketplace, and by this we can finance the servers, do some marketing and maybe even pay authors one day (however, not sure if that's the point of an open wiki.. what do you guys think? I am still uncertain about that point).

If you are interested, I would be happy to show you around the Wiki and we can see if we do something together! Basically, we could either set up a "menu point" on synbio.info for plant biology or even start an own wiki, as we recently did for bio art at http://art.synbio.info. What do you think? 

Talk to you soon and best regards from way too cold Munich, 

Jérôme



Am Samstag, 28. November 2015 19:21:57 UTC+1 schrieb Sebastian:
Hi Everyone,
I've been bouncing around the idea of compiling the 7+ years of plant tissue culture experience I've mustered into articles for my blog for a long time now. I have a ton of content as drafts and galleries of original pictures and whatnot and am stuck at a crossroads and would like some advice. My plan was to keep posting small articles to my blog about the little steps needed to make a transgenic plant in very newbie-friendly detail. A lot of my contacts recommended I just compile a book and publish it so that I can get a little compensation for the information that would be a little hard to come by in that specific format. I have always put teaching people first and tend to forget about supporting myself which is what led me to this intersection. 

Would people rather have a nice free blog that steadily outputs content with some tasteful adds pertaining to relevant companies and products, or pay a small price for a Julia Child-style cookbook of plant tissue culture and transformation recipes concocted by yours truly with plenty of pictures and commentary?

 What would be a good price point?

 Is it worth the delay to have a physical edition one can tote to their bench or is it more useful to have an organized blog website thingy that people can reference digitally?

What would be a good starting project? I was thinking a GUS assay since it covers a bunch of various topics and the plasmid is commercially available and off-patent. I'm not a fan of GFP since the stable ones require harmful UV and I have yet to transform the couple fluorescent plasmids I've optimized into tobacco to verify that long wave UV will work with decent effect. Another option would be anthocyanin knockouts/add-ins or variegation gene introduction but would require further testing. GUS requires a bit more chemicals but the result is a strong insoluble blue precipitate against a white background of ethanol-bleached plants. No fancy equipment necessary! I'm also not stepping foot anywhere near glowing plants for many reasons so please don't ask to make a book about making a glowing plant from scratch. That being said, if you had a plant biotech cookbook, what would be your desired "Hello World" project? Herbicide tolerance alone is boring, non-visual, and controversial/unethical in spread so that's out of the question. It's kinda funny that the perfect intro project would require a lot of research on my part into verifying chromoprotein stability in lower pH (plant cytosol is ~5.6 - 5.8) and shipping things to the vacuole, etc, etc. so any feedback on what you all want to have as the main project of the book (if you even want a book in the first place) would be awesome!

I thought I would ask the community that would be most interested in learning more about the dark-green arts (you gals and guys) if it would even be worth the trouble. I've noticed an overwhelming lack of good plant biotech material out there and there are too many comments about plant culturing being "hard to do" and "unpredictable" which I think is just silly. Anyone can do science and everyone can do plant biotech. The hardest part seems to be aseptic technique and culture-to-culture media decisions but those are the most straight forward. I am a very visual learner so I would include a ton of pictures with various angles and explanations so anyone ages ~8 and up (maybe even earlier if fine motor skills are honed!) can make their own plants. Either way let me know what y'all think about the idea of Book vs Blog for introductory plant tissue culture. I would still maintain my blog for the day to day content and more advanced topics but for the newbie I think a desk reference at a fair price would be ideal. Thanks!

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Re: [DIYbio] Book or Blog?

I have found that publishing first on the web, and then in a paper book, increases sales of the paper book considerably.
An earlier book came out of this web site.

I like to get paid several times for the same work.
I describe how to build something on the web, and I get advertising revenue.
I sell that description as an article to Make magazine, and get paid again.
When enough projects are ready, we collect them into a printed book, and I get paid yet again.
And I sell parts to build the projects on the web site.

All of this revenue goes back into inventing more projects for the web sites.

I recommend this strategy to everyone on the list. Getting paid helps to fund more projects, and helps to keep up enthusiasm for the work.
And web sites, printed articles and books help us all.


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On Sat, Nov 28, 2015 at 11:11 AM, Sebastian S Cocioba <scocioba@gmail.com> wrote:
Valid point Meredith. Thanks for the tip! I just want to make sure the content is as accessible as possible and if It could also pay for media, tips, and coffee that would be great aswell!

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC


On Nov 28, 2015, at 2:06 PM, Meredith L. Patterson <clonearmy@gmail.com> wrote:

You could do both. No Starch Press has published a couple of books that started out as blogs and still remain free online, e.g. http://learnyouahaskell.com/. Bill Pollock, who runs No Starch, is super friendly and approachable -- drop him an email and ask?

Cheers,
--mlp

On Sat, Nov 28, 2015 at 7:24 PM, Dakota Hamill <dkotes@gmail.com> wrote:

Get paid for your time, write a book.

On Nov 28, 2015 1:21 PM, "Sebastian Cocioba" <scocioba@gmail.com> wrote:
Hi Everyone,
I've been bouncing around the idea of compiling the 7+ years of plant tissue culture experience I've mustered into articles for my blog for a long time now. I have a ton of content as drafts and galleries of original pictures and whatnot and am stuck at a crossroads and would like some advice. My plan was to keep posting small articles to my blog about the little steps needed to make a transgenic plant in very newbie-friendly detail. A lot of my contacts recommended I just compile a book and publish it so that I can get a little compensation for the information that would be a little hard to come by in that specific format. I have always put teaching people first and tend to forget about supporting myself which is what led me to this intersection. 

Would people rather have a nice free blog that steadily outputs content with some tasteful adds pertaining to relevant companies and products, or pay a small price for a Julia Child-style cookbook of plant tissue culture and transformation recipes concocted by yours truly with plenty of pictures and commentary?

 What would be a good price point?

 Is it worth the delay to have a physical edition one can tote to their bench or is it more useful to have an organized blog website thingy that people can reference digitally?

What would be a good starting project? I was thinking a GUS assay since it covers a bunch of various topics and the plasmid is commercially available and off-patent. I'm not a fan of GFP since the stable ones require harmful UV and I have yet to transform the couple fluorescent plasmids I've optimized into tobacco to verify that long wave UV will work with decent effect. Another option would be anthocyanin knockouts/add-ins or variegation gene introduction but would require further testing. GUS requires a bit more chemicals but the result is a strong insoluble blue precipitate against a white background of ethanol-bleached plants. No fancy equipment necessary! I'm also not stepping foot anywhere near glowing plants for many reasons so please don't ask to make a book about making a glowing plant from scratch. That being said, if you had a plant biotech cookbook, what would be your desired "Hello World" project? Herbicide tolerance alone is boring, non-visual, and controversial/unethical in spread so that's out of the question. It's kinda funny that the perfect intro project would require a lot of research on my part into verifying chromoprotein stability in lower pH (plant cytosol is ~5.6 - 5.8) and shipping things to the vacuole, etc, etc. so any feedback on what you all want to have as the main project of the book (if you even want a book in the first place) would be awesome!

I thought I would ask the community that would be most interested in learning more about the dark-green arts (you gals and guys) if it would even be worth the trouble. I've noticed an overwhelming lack of good plant biotech material out there and there are too many comments about plant culturing being "hard to do" and "unpredictable" which I think is just silly. Anyone can do science and everyone can do plant biotech. The hardest part seems to be aseptic technique and culture-to-culture media decisions but those are the most straight forward. I am a very visual learner so I would include a ton of pictures with various angles and explanations so anyone ages ~8 and up (maybe even earlier if fine motor skills are honed!) can make their own plants. Either way let me know what y'all think about the idea of Book vs Blog for introductory plant tissue culture. I would still maintain my blog for the day to day content and more advanced topics but for the newbie I think a desk reference at a fair price would be ideal. Thanks!

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Re: [DIYbio] Book or Blog?

Valid point Meredith. Thanks for the tip! I just want to make sure the content is as accessible as possible and if It could also pay for media, tips, and coffee that would be great aswell!

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC


On Nov 28, 2015, at 2:06 PM, Meredith L. Patterson <clonearmy@gmail.com> wrote:

You could do both. No Starch Press has published a couple of books that started out as blogs and still remain free online, e.g. http://learnyouahaskell.com/. Bill Pollock, who runs No Starch, is super friendly and approachable -- drop him an email and ask?

Cheers,
--mlp

On Sat, Nov 28, 2015 at 7:24 PM, Dakota Hamill <dkotes@gmail.com> wrote:

Get paid for your time, write a book.

On Nov 28, 2015 1:21 PM, "Sebastian Cocioba" <scocioba@gmail.com> wrote:
Hi Everyone,
I've been bouncing around the idea of compiling the 7+ years of plant tissue culture experience I've mustered into articles for my blog for a long time now. I have a ton of content as drafts and galleries of original pictures and whatnot and am stuck at a crossroads and would like some advice. My plan was to keep posting small articles to my blog about the little steps needed to make a transgenic plant in very newbie-friendly detail. A lot of my contacts recommended I just compile a book and publish it so that I can get a little compensation for the information that would be a little hard to come by in that specific format. I have always put teaching people first and tend to forget about supporting myself which is what led me to this intersection. 

Would people rather have a nice free blog that steadily outputs content with some tasteful adds pertaining to relevant companies and products, or pay a small price for a Julia Child-style cookbook of plant tissue culture and transformation recipes concocted by yours truly with plenty of pictures and commentary?

 What would be a good price point?

 Is it worth the delay to have a physical edition one can tote to their bench or is it more useful to have an organized blog website thingy that people can reference digitally?

What would be a good starting project? I was thinking a GUS assay since it covers a bunch of various topics and the plasmid is commercially available and off-patent. I'm not a fan of GFP since the stable ones require harmful UV and I have yet to transform the couple fluorescent plasmids I've optimized into tobacco to verify that long wave UV will work with decent effect. Another option would be anthocyanin knockouts/add-ins or variegation gene introduction but would require further testing. GUS requires a bit more chemicals but the result is a strong insoluble blue precipitate against a white background of ethanol-bleached plants. No fancy equipment necessary! I'm also not stepping foot anywhere near glowing plants for many reasons so please don't ask to make a book about making a glowing plant from scratch. That being said, if you had a plant biotech cookbook, what would be your desired "Hello World" project? Herbicide tolerance alone is boring, non-visual, and controversial/unethical in spread so that's out of the question. It's kinda funny that the perfect intro project would require a lot of research on my part into verifying chromoprotein stability in lower pH (plant cytosol is ~5.6 - 5.8) and shipping things to the vacuole, etc, etc. so any feedback on what you all want to have as the main project of the book (if you even want a book in the first place) would be awesome!

I thought I would ask the community that would be most interested in learning more about the dark-green arts (you gals and guys) if it would even be worth the trouble. I've noticed an overwhelming lack of good plant biotech material out there and there are too many comments about plant culturing being "hard to do" and "unpredictable" which I think is just silly. Anyone can do science and everyone can do plant biotech. The hardest part seems to be aseptic technique and culture-to-culture media decisions but those are the most straight forward. I am a very visual learner so I would include a ton of pictures with various angles and explanations so anyone ages ~8 and up (maybe even earlier if fine motor skills are honed!) can make their own plants. Either way let me know what y'all think about the idea of Book vs Blog for introductory plant tissue culture. I would still maintain my blog for the day to day content and more advanced topics but for the newbie I think a desk reference at a fair price would be ideal. Thanks!

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Re: [DIYbio] Book or Blog?

You could do both. No Starch Press has published a couple of books that started out as blogs and still remain free online, e.g. http://learnyouahaskell.com/. Bill Pollock, who runs No Starch, is super friendly and approachable -- drop him an email and ask?

Cheers,
--mlp

On Sat, Nov 28, 2015 at 7:24 PM, Dakota Hamill <dkotes@gmail.com> wrote:

Get paid for your time, write a book.

On Nov 28, 2015 1:21 PM, "Sebastian Cocioba" <scocioba@gmail.com> wrote:
Hi Everyone,
I've been bouncing around the idea of compiling the 7+ years of plant tissue culture experience I've mustered into articles for my blog for a long time now. I have a ton of content as drafts and galleries of original pictures and whatnot and am stuck at a crossroads and would like some advice. My plan was to keep posting small articles to my blog about the little steps needed to make a transgenic plant in very newbie-friendly detail. A lot of my contacts recommended I just compile a book and publish it so that I can get a little compensation for the information that would be a little hard to come by in that specific format. I have always put teaching people first and tend to forget about supporting myself which is what led me to this intersection. 

Would people rather have a nice free blog that steadily outputs content with some tasteful adds pertaining to relevant companies and products, or pay a small price for a Julia Child-style cookbook of plant tissue culture and transformation recipes concocted by yours truly with plenty of pictures and commentary?

 What would be a good price point?

 Is it worth the delay to have a physical edition one can tote to their bench or is it more useful to have an organized blog website thingy that people can reference digitally?

What would be a good starting project? I was thinking a GUS assay since it covers a bunch of various topics and the plasmid is commercially available and off-patent. I'm not a fan of GFP since the stable ones require harmful UV and I have yet to transform the couple fluorescent plasmids I've optimized into tobacco to verify that long wave UV will work with decent effect. Another option would be anthocyanin knockouts/add-ins or variegation gene introduction but would require further testing. GUS requires a bit more chemicals but the result is a strong insoluble blue precipitate against a white background of ethanol-bleached plants. No fancy equipment necessary! I'm also not stepping foot anywhere near glowing plants for many reasons so please don't ask to make a book about making a glowing plant from scratch. That being said, if you had a plant biotech cookbook, what would be your desired "Hello World" project? Herbicide tolerance alone is boring, non-visual, and controversial/unethical in spread so that's out of the question. It's kinda funny that the perfect intro project would require a lot of research on my part into verifying chromoprotein stability in lower pH (plant cytosol is ~5.6 - 5.8) and shipping things to the vacuole, etc, etc. so any feedback on what you all want to have as the main project of the book (if you even want a book in the first place) would be awesome!

I thought I would ask the community that would be most interested in learning more about the dark-green arts (you gals and guys) if it would even be worth the trouble. I've noticed an overwhelming lack of good plant biotech material out there and there are too many comments about plant culturing being "hard to do" and "unpredictable" which I think is just silly. Anyone can do science and everyone can do plant biotech. The hardest part seems to be aseptic technique and culture-to-culture media decisions but those are the most straight forward. I am a very visual learner so I would include a ton of pictures with various angles and explanations so anyone ages ~8 and up (maybe even earlier if fine motor skills are honed!) can make their own plants. Either way let me know what y'all think about the idea of Book vs Blog for introductory plant tissue culture. I would still maintain my blog for the day to day content and more advanced topics but for the newbie I think a desk reference at a fair price would be ideal. Thanks!

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Re: [DIYbio] Book or Blog?

Get paid for your time, write a book.

On Nov 28, 2015 1:21 PM, "Sebastian Cocioba" <scocioba@gmail.com> wrote:
Hi Everyone,
I've been bouncing around the idea of compiling the 7+ years of plant tissue culture experience I've mustered into articles for my blog for a long time now. I have a ton of content as drafts and galleries of original pictures and whatnot and am stuck at a crossroads and would like some advice. My plan was to keep posting small articles to my blog about the little steps needed to make a transgenic plant in very newbie-friendly detail. A lot of my contacts recommended I just compile a book and publish it so that I can get a little compensation for the information that would be a little hard to come by in that specific format. I have always put teaching people first and tend to forget about supporting myself which is what led me to this intersection. 

Would people rather have a nice free blog that steadily outputs content with some tasteful adds pertaining to relevant companies and products, or pay a small price for a Julia Child-style cookbook of plant tissue culture and transformation recipes concocted by yours truly with plenty of pictures and commentary?

 What would be a good price point?

 Is it worth the delay to have a physical edition one can tote to their bench or is it more useful to have an organized blog website thingy that people can reference digitally?

What would be a good starting project? I was thinking a GUS assay since it covers a bunch of various topics and the plasmid is commercially available and off-patent. I'm not a fan of GFP since the stable ones require harmful UV and I have yet to transform the couple fluorescent plasmids I've optimized into tobacco to verify that long wave UV will work with decent effect. Another option would be anthocyanin knockouts/add-ins or variegation gene introduction but would require further testing. GUS requires a bit more chemicals but the result is a strong insoluble blue precipitate against a white background of ethanol-bleached plants. No fancy equipment necessary! I'm also not stepping foot anywhere near glowing plants for many reasons so please don't ask to make a book about making a glowing plant from scratch. That being said, if you had a plant biotech cookbook, what would be your desired "Hello World" project? Herbicide tolerance alone is boring, non-visual, and controversial/unethical in spread so that's out of the question. It's kinda funny that the perfect intro project would require a lot of research on my part into verifying chromoprotein stability in lower pH (plant cytosol is ~5.6 - 5.8) and shipping things to the vacuole, etc, etc. so any feedback on what you all want to have as the main project of the book (if you even want a book in the first place) would be awesome!

I thought I would ask the community that would be most interested in learning more about the dark-green arts (you gals and guys) if it would even be worth the trouble. I've noticed an overwhelming lack of good plant biotech material out there and there are too many comments about plant culturing being "hard to do" and "unpredictable" which I think is just silly. Anyone can do science and everyone can do plant biotech. The hardest part seems to be aseptic technique and culture-to-culture media decisions but those are the most straight forward. I am a very visual learner so I would include a ton of pictures with various angles and explanations so anyone ages ~8 and up (maybe even earlier if fine motor skills are honed!) can make their own plants. Either way let me know what y'all think about the idea of Book vs Blog for introductory plant tissue culture. I would still maintain my blog for the day to day content and more advanced topics but for the newbie I think a desk reference at a fair price would be ideal. Thanks!

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