Re: [DIYbio] Re: free food

Teach them to sprout beans and lentils.
Then teach them to grow mushrooms.  Read books by Paul Stamets.
Problem solved.
Too bad welfare systems of the modern world don't also take this approach, it is not only a third-world limitation.

Forget algae, it does not have enough calories per dry kg with dense enough growing volume.  Too much water and too much ground space required - which is why it has failed to scale, except in land regions next to large lakes or oceans [Aztecs anyone?].


## Jonathan Cline
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On Monday, January 19, 2015 at 6:54:21 PM UTC-8, alex smith wrote:
first get cheap water for them
second help them grow more with less water


2015-01-20 10:40 GMT+08:00 Nathan McCorkle <nmz...@gmail.com>:
On Mon, Jan 19, 2015 at 5:40 PM, 'SC' via DIYbio
<diy...@googlegroups.com> wrote:
> If they have enough water to grow algae, they would have
> enough water to grow something else.

Unless they are growing in brackish water.
 

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Re: [DIYbio] DIY Composting toilets & California's worst drought ever

That's pretty cool.  I assume your water has been tested?  You could be getting some good nitrate liquid fertilizers out of the deal too with some upgrades.  The other aspect of hacking toilets of course is to add biomedical sensors.  Some Japanese vendors have had fancy smart-toilets in the market for years which attempt these features.  I'm not too familiar with the success rates of their technology.  It is definitely an area that is overlooked in the diy area and in the west in general.


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On Wednesday, March 25, 2015 at 3:46:54 PM UTC-7, Simon Field wrote:
I have a guilt-free flushing toilet.
I have a 310 foot deep well.
I pump the water up using solar power.
I have a leach field six feet deep, where the septic overflow goes.

So I pump water from 310 feet, and put it back into the ground under the meadow in front of my house. After a few years of percolating through rock and sand, it ends up in the Silicon Valley water supply, just like the water would have if I hadn't pumped it from the well.

:-)

 

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Re: [DIYbio] Completely new to DIY gene therapy

There's peeps working on it or at a minimum looking at it.   Some people clearly have no common sense as can be seen by the body modifier personalities already injecting useless semiconductor chips subdermally into their hands, then bragging about it.. others injecting even worse in the false name of biohacking.  Then again there are biologists who smoke cigarettes so science degrees and common sense do not always go together.


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On Thursday, March 26, 2015 at 1:43:24 PM UTC-7, Cathal Garvey wrote:
DIY Gene Therapy is not, to my knowledge, a "thing" right now, nor
should it be.

As someone who studied nonviral integrative gene therapy several years
back for cancer therapeutic uses, I can tell you that there is no clear
consensus on how to efficiently transfect DNA into live tissue without
incurring a *significant* risk of inducing either cancer or a
catastrophic immune backlash. There have been gene therapy trials that
ended in the sudden deaths of participants due to unforeseen cytokine
storms that *did not occur in prior animal trials*.

Gene therapy is a potentially amazing medical technology, but it was
invented and first trialled over a decade ago, and is not yet a
recommended or permitted therapy for anything that I am aware of, and
the primary reason is that it is not, at all, safe.
 

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Re: [DIYbio] diy MRSA antibiotic using garlic, reproduced in lab

I see what you're saying yet if it's so obvious or unsurprising then presumably it should have been published within the past couple decades already (or has it? Not that I remember.).  MRSA is important.   Raise your hand if you've been prescribed the typical metabolic-nuclear-assault antibiotics that you didn't want or possibly did not need. 

Oh right, a simple garlic combo mix wouldn't be investigated because:  "Let's not work on that, it's not novel enough", or, "There's no patent opportunities here, there's no new I.P.",  or, "That type of work is a dead end."   The same "lack of characterization" problem of biotech.   Not sure where the supposed billions of R&D dollars searching for new antibiotics is going, eh?   The video article mentions the key phrases from lab members:  "We didn't expect anything" ...  "This was a side project for the lab".  Of course this article could be headline fluff or university P. R. hype, won't know until refs are scrutinized so it's best not to take any of it too seriously until then.


(Though anyway, I'm a dude who chops my garlic and leaves it sitting on the counter for several minutes before cooking with it to increase magic and even then I add it at the end so as to not expose it to significant cooking heat, under auspices that it's reducing my statistical risk for typical bad things, so whatta I know.  -- Ref,  I think, maybe in here: Analytical Sciences Vol. 25 (2009) , No. 1 p.137, by Yanbei ZHU, Kazumi INAGAKI, Hiroki HARAGUCHI and Koichi CHIBA.)


A related factoid here is that I didn't know otherwise healthy people would want to regularly take bovine bile acid supplements.    Eww.  


## Jonathan Cline
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On Tuesday, March 31, 2015 at 5:04:58 PM UTC-7, alexander hollins wrote:
Yeah.. I'm not sure what the surprise is.  It reminds me of George Carlin bit about "happens to be black".   Allicin is one of the first sulfa antibiotics we used, and is a direct extract from Garlic.  With the salts and such that are in there, you're basically breaking down the fats and protiens and extracting it, and Sulfa drugs have already been shown to be amazingly effective on Mersa (and yet no one prescribes it... sigh)

The wonder of the whole thing is how detailed the process is for the time frame, not that it works.  

On Tue, Mar 31, 2015 at 4:46 PM, Jonathan Cline <jcl...@ieee.org> wrote:
Today's big pharma ain't got nuthin on 9th century Anglo-Saxon diybio?

 

""A one thousand year old Anglo-Saxon remedy for eye infections which originates from a manuscript in the British Library has been found to kill the modern-day superbug MRSA in an unusual research collaboration at The University of Nottingham.""

Bald's eye salve [as listed in BBC news article, see ref below]:

Equal amounts of garlic and another allium (onion or leek), finely chopped and crushed in a mortar for two minutes.

Add 25ml (0.87 fl oz) of English wine - taken from a historic vineyard near Glastonbury.

Dissolve bovine salts in distilled water, add and then keep chilled for nine days at 4C.



...
"" Early results on the 'potion', tested in vitro at Nottingham and backed up by mouse model tests at a university in the United States, are, in the words of the US collaborator, "astonishing". The solution has had remarkable effects on Methicillin-resistant Staphylococcus aureus (MRSA) which is one of the most antibiotic-resistant bugs costing modern health services billions. ""

 

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Re: [DIYbio] diy MRSA antibiotic using garlic, reproduced in lab

Yes, some people with sulfa allergies have issues with garlic.  

On Tue, Mar 31, 2015 at 5:15 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
On Tue, Mar 31, 2015 at 5:04 PM, leaking pen <itsatrap@gmail.com> wrote:
> Yeah.. I'm not sure what the surprise is.  It reminds me of George Carlin
> bit about "happens to be black".   Allicin is one of the first sulfa
> antibiotics we used, and is a direct extract from Garlic.

Does that mean people with sulfa drug allergies can't eat garlic, or
likely don't like to eat it?

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Re: [DIYbio] diy MRSA antibiotic using garlic, reproduced in lab

iirc it kills up to 90%.

> On Mar 31, 2015, at 6:17 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
>
>> On Tue, Mar 31, 2015 at 6:14 PM, Jonathan Cline <jcline@ieee.org> wrote:
>>
>> formal testing continues. Meanwhile, you might also like to try this recipe
>> as a tasty spread on a nice baguette with a bit of oil."
>
> Formal /tasting/ can also proceed.
>
> Not to mention all the marinades you could make...
>
>
> --
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>
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Re: [DIYbio] diy MRSA antibiotic using garlic, reproduced in lab

Of course, the way sulfa drugs work is that they mimic the B vitamin para amino benzoic acid (PABA). The bacteria take it up instead of the vitamin, and they can't live without PABA.

So the mix might not work unless you stop getting PABA. And if you eat a lot of the mix, you might want to make sure you are getting enough PABA (unless you have an infection...).

A similar antibiotic approach is used by chickens to prevent bacterial spoilage of eggs. Chicken eggs contain large amounts of avidin in the raw egg white. Avidin binds to the B vitamin biotin, so the bacteria can't get any. Cook your egg whites to denature the avidin.

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On Tue, Mar 31, 2015 at 6:17 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
On Tue, Mar 31, 2015 at 6:14 PM, Jonathan Cline <jcline@ieee.org> wrote:

> formal testing continues.  Meanwhile, you might also like to try this recipe
> as a tasty spread on a nice baguette with a bit of oil."

Formal /tasting/ can also proceed.

Not to mention all the marinades you could make...


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Re: [DIYbio] diy MRSA antibiotic using garlic, reproduced in lab

On Tue, Mar 31, 2015 at 6:14 PM, Jonathan Cline <jcline@ieee.org> wrote:

> formal testing continues. Meanwhile, you might also like to try this recipe
> as a tasty spread on a nice baguette with a bit of oil."

Formal /tasting/ can also proceed.

Not to mention all the marinades you could make...


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Re: [DIYbio] diy MRSA antibiotic using garlic, reproduced in lab

Testing in the U.S. included skin infections on mice [ref video http://www.bbc.com/news/health-32124642 ]

Curiously the news report concludes: "One day, this could lead to a new drug" .. what?   I'm perfectly fine with putting the garlic, onion, a couple drops of  cholic acid, and a two-buck chuck mash on a skin infection.   It's vegan after all :-P  and cheaper than pharmacare.   At least it's the wrong conclusion for the news article in my opinion.  A truly wondrous conclusion would be: "We're releasing a simplified recipe on our web site which lists grocery store equivalents so others can experiment with the recipe, while formal testing continues.  Meanwhile, you might also like to try this recipe as a tasty spread on a nice baguette with a bit of oil."

## Jonathan Cline  ## jcline@ieee.org  ## Mobile: +1-805-617-0223  ########################    
On 3/31/15 5:52 PM, Simon Quellen Field wrote:
That will teach them to get eye infections.
Let's make a concoction out of garlic and onion juice, wine, and some cow digestive juices and put that in their eye. I bet they don't come back complaining about any eye infections anytime soon.

I'll also bet there are lots of other things that kill MRSA in vitro.
Sulfuric acid come to mind. Or just a lot of salt and alcohol.
:-)


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On Tue, Mar 31, 2015 at 4:46 PM, Jonathan Cline <jcline@ieee.org> wrote:
Today's big pharma ain't got nuthin on 9th century Anglo-Saxon diybio?

 

""A one thousand year old Anglo-Saxon remedy for eye infections which originates from a manuscript in the British Library has been found to kill the modern-day superbug MRSA in an unusual research collaboration at The University of Nottingham.""

Bald's eye salve [as listed in BBC news article, see ref below]:

Equal amounts of garlic and another allium (onion or leek), finely chopped and crushed in a mortar for two minutes.

Add 25ml (0.87 fl oz) of English wine - taken from a historic vineyard near Glastonbury.

Dissolve bovine salts in distilled water, add and then keep chilled for nine days at 4C.



...
"" Early results on the 'potion', tested in vitro at Nottingham and backed up by mouse model tests at a university in the United States, are, in the words of the US collaborator, "astonishing". The solution has had remarkable effects on Methicillin-resistant Staphylococcus aureus (MRSA) which is one of the most antibiotic-resistant bugs costing modern health services billions. ""

Ref:  University press release
http://www.nottingham.ac.uk/news/pressreleases/2015/march/ancientbiotics---a-medieval-remedy-for-modern-day-superbugs.aspx


BBC News article

http://www.bbc.com/news/uk-england-nottinghamshire-32117815

""" A 1,000-year-old treatment for eye infections could hold the key to killing antibiotic-resistant superbugs, experts have said. Scientists recreated a 9th Century Anglo-Saxon remedy using onion, garlic and part of a cow's stomach. They were "astonished" to find it almost completely wiped out methicillin-resistant staphylococcus aureus, otherwise known as MRSA. Their findings will be presented at a national microbiology conference. The remedy was found in Bald's Leechbook - an old English manuscript containing instructions on various treatments held in the British Library. Anglo-Saxon expert Dr Christina Lee, from the University of Nottingham, translated the recipe for an "eye salve", which includes garlic, onion or leeks, wine and cow bile."""
...

"" The team's findings will be presented at the Annual Conference of the Society for General Microbiology, in Birmingham."

 

## Jonathan Cline  ## jcline@ieee.org  ## Mobile: +1-805-617-0223  ########################    
 

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Re: [DIYbio] diy MRSA antibiotic using garlic, reproduced in lab

That will teach them to get eye infections.
Let's make a concoction out of garlic and onion juice, wine, and some cow digestive juices and put that in their eye. I bet they don't come back complaining about any eye infections anytime soon.

I'll also bet there are lots of other things that kill MRSA in vitro.
Sulfuric acid come to mind. Or just a lot of salt and alcohol.
:-)


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On Tue, Mar 31, 2015 at 4:46 PM, Jonathan Cline <jcline@ieee.org> wrote:
Today's big pharma ain't got nuthin on 9th century Anglo-Saxon diybio?

 

""A one thousand year old Anglo-Saxon remedy for eye infections which originates from a manuscript in the British Library has been found to kill the modern-day superbug MRSA in an unusual research collaboration at The University of Nottingham.""

Bald's eye salve [as listed in BBC news article, see ref below]:

Equal amounts of garlic and another allium (onion or leek), finely chopped and crushed in a mortar for two minutes.

Add 25ml (0.87 fl oz) of English wine - taken from a historic vineyard near Glastonbury.

Dissolve bovine salts in distilled water, add and then keep chilled for nine days at 4C.



...
"" Early results on the 'potion', tested in vitro at Nottingham and backed up by mouse model tests at a university in the United States, are, in the words of the US collaborator, "astonishing". The solution has had remarkable effects on Methicillin-resistant Staphylococcus aureus (MRSA) which is one of the most antibiotic-resistant bugs costing modern health services billions. ""

Ref:  University press release
http://www.nottingham.ac.uk/news/pressreleases/2015/march/ancientbiotics---a-medieval-remedy-for-modern-day-superbugs.aspx


BBC News article

http://www.bbc.com/news/uk-england-nottinghamshire-32117815

""" A 1,000-year-old treatment for eye infections could hold the key to killing antibiotic-resistant superbugs, experts have said. Scientists recreated a 9th Century Anglo-Saxon remedy using onion, garlic and part of a cow's stomach. They were "astonished" to find it almost completely wiped out methicillin-resistant staphylococcus aureus, otherwise known as MRSA. Their findings will be presented at a national microbiology conference. The remedy was found in Bald's Leechbook - an old English manuscript containing instructions on various treatments held in the British Library. Anglo-Saxon expert Dr Christina Lee, from the University of Nottingham, translated the recipe for an "eye salve", which includes garlic, onion or leeks, wine and cow bile."""
...

"" The team's findings will be presented at the Annual Conference of the Society for General Microbiology, in Birmingham."

 

## Jonathan Cline  ## jcline@ieee.org  ## Mobile: +1-805-617-0223  ########################       

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Re: [DIYbio] diy MRSA antibiotic using garlic, reproduced in lab

On Tue, Mar 31, 2015 at 5:04 PM, leaking pen <itsatrap@gmail.com> wrote:
> Yeah.. I'm not sure what the surprise is. It reminds me of George Carlin
> bit about "happens to be black". Allicin is one of the first sulfa
> antibiotics we used, and is a direct extract from Garlic.

Does that mean people with sulfa drug allergies can't eat garlic, or
likely don't like to eat it?

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Re: [DIYbio] diy MRSA antibiotic using garlic, reproduced in lab

Yeah.. I'm not sure what the surprise is.  It reminds me of George Carlin bit about "happens to be black".   Allicin is one of the first sulfa antibiotics we used, and is a direct extract from Garlic.  With the salts and such that are in there, you're basically breaking down the fats and protiens and extracting it, and Sulfa drugs have already been shown to be amazingly effective on Mersa (and yet no one prescribes it... sigh)

The wonder of the whole thing is how detailed the process is for the time frame, not that it works.  

On Tue, Mar 31, 2015 at 4:46 PM, Jonathan Cline <jcline@ieee.org> wrote:
Today's big pharma ain't got nuthin on 9th century Anglo-Saxon diybio?

 

""A one thousand year old Anglo-Saxon remedy for eye infections which originates from a manuscript in the British Library has been found to kill the modern-day superbug MRSA in an unusual research collaboration at The University of Nottingham.""

Bald's eye salve [as listed in BBC news article, see ref below]:

Equal amounts of garlic and another allium (onion or leek), finely chopped and crushed in a mortar for two minutes.

Add 25ml (0.87 fl oz) of English wine - taken from a historic vineyard near Glastonbury.

Dissolve bovine salts in distilled water, add and then keep chilled for nine days at 4C.



...
"" Early results on the 'potion', tested in vitro at Nottingham and backed up by mouse model tests at a university in the United States, are, in the words of the US collaborator, "astonishing". The solution has had remarkable effects on Methicillin-resistant Staphylococcus aureus (MRSA) which is one of the most antibiotic-resistant bugs costing modern health services billions. ""

Ref:  University press release
http://www.nottingham.ac.uk/news/pressreleases/2015/march/ancientbiotics---a-medieval-remedy-for-modern-day-superbugs.aspx


BBC News article

http://www.bbc.com/news/uk-england-nottinghamshire-32117815

""" A 1,000-year-old treatment for eye infections could hold the key to killing antibiotic-resistant superbugs, experts have said. Scientists recreated a 9th Century Anglo-Saxon remedy using onion, garlic and part of a cow's stomach. They were "astonished" to find it almost completely wiped out methicillin-resistant staphylococcus aureus, otherwise known as MRSA. Their findings will be presented at a national microbiology conference. The remedy was found in Bald's Leechbook - an old English manuscript containing instructions on various treatments held in the British Library. Anglo-Saxon expert Dr Christina Lee, from the University of Nottingham, translated the recipe for an "eye salve", which includes garlic, onion or leeks, wine and cow bile."""
...

"" The team's findings will be presented at the Annual Conference of the Society for General Microbiology, in Birmingham."

 

## Jonathan Cline  ## jcline@ieee.org  ## Mobile: +1-805-617-0223  ########################       

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[DIYbio] Re: Biohacking gray hair

You geniuses do know that some people just never go grey. Right?

On Tuesday, March 10, 2015 at 12:40:56 PM UTC-4, Mike Petersen wrote:
Hi DIYbio community,

I was wondering if there could be any "system-biological" approach to "cure gray hair" and prevent the hair from losing its colour.
What is known about the genetic and the epigenetic mechanisms that cause the colour loss of our hair?
There are some interesting patterns, for example the fact that usually the hair around the temples starts to lose its colors first.
Which expression patterns can be linked to these observations?
And what would be promising ideas to interact with these mechanisms?

Thank you for your ideas

Mike

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[DIYbio] diy MRSA antibiotic using garlic, reproduced in lab

Today's big pharma ain't got nuthin on 9th century Anglo-Saxon diybio?

 

""A one thousand year old Anglo-Saxon remedy for eye infections which originates from a manuscript in the British Library has been found to kill the modern-day superbug MRSA in an unusual research collaboration at The University of Nottingham.""

Bald's eye salve [as listed in BBC news article, see ref below]:

Equal amounts of garlic and another allium (onion or leek), finely chopped and crushed in a mortar for two minutes.

Add 25ml (0.87 fl oz) of English wine - taken from a historic vineyard near Glastonbury.

Dissolve bovine salts in distilled water, add and then keep chilled for nine days at 4C.



...
"" Early results on the 'potion', tested in vitro at Nottingham and backed up by mouse model tests at a university in the United States, are, in the words of the US collaborator, "astonishing". The solution has had remarkable effects on Methicillin-resistant Staphylococcus aureus (MRSA) which is one of the most antibiotic-resistant bugs costing modern health services billions. ""

Ref:  University press release
http://www.nottingham.ac.uk/news/pressreleases/2015/march/ancientbiotics---a-medieval-remedy-for-modern-day-superbugs.aspx


BBC News article

http://www.bbc.com/news/uk-england-nottinghamshire-32117815

""" A 1,000-year-old treatment for eye infections could hold the key to killing antibiotic-resistant superbugs, experts have said. Scientists recreated a 9th Century Anglo-Saxon remedy using onion, garlic and part of a cow's stomach. They were "astonished" to find it almost completely wiped out methicillin-resistant staphylococcus aureus, otherwise known as MRSA. Their findings will be presented at a national microbiology conference. The remedy was found in Bald's Leechbook - an old English manuscript containing instructions on various treatments held in the British Library. Anglo-Saxon expert Dr Christina Lee, from the University of Nottingham, translated the recipe for an "eye salve", which includes garlic, onion or leeks, wine and cow bile."""
...

"" The team's findings will be presented at the Annual Conference of the Society for General Microbiology, in Birmingham."

 

## Jonathan Cline  ## jcline@ieee.org  ## Mobile: +1-805-617-0223  ########################       

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Urgent Client Need......... Java UI Developer

Hi ,

 

Need GC/GC-EAD/TN/US Citizens Only

 

Position: Java UI Developer

Location: Wilmington, DE

Duration: 21 months Contract

 

Preferred Qualifications:

·         HTML5, AngularJS

·         Java, Spring, Web Services

 

Job Description: 

5+ years experience with Financial Services clients.

Oversight.

Coordinating others work while involved with multiple work streams.

 

Related experience:

·         3-5 years prior application development experience.

·         Client experience desired.

·         Demonstrated experience with large scale application integration efforts.

·         Have participated in more than 5 Agile projects.

·         Communication - Ability to communicate strategies and processes around data modeling and architecture to cross functional groups and senior levels.

·         Ability to influence multiple levels on highly technical issues and challenges.

·         Demonstrated experience to influence and coordinate third parties and suppliers.

 


If you are interested, please send resume to sarat@saisoftsol.com

 

Thanks & Regards,

 

Sarat Kumar

Technical Recruiter

Sai Software Solutions, LLC

2942 N 24th Street,

Phoenix, AZ - 85016

Off       :  602-666-6356

e-mail : sarat@saisoftsol.com

web     : www.saisoftsol.com

 


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[DIYbio] Re: Chemical Structure Generation for Drug Simulation

AFAIK there's no API for that. If there were, I suspect it would be forced to make a lot of assumptions that would sacrifice a significant deal of accuracy for speed and ease-of-use. Typically this type of experiment requires a good deal of hand-holding, as there are decisions that need to be made wisely at many steps of the project.

For instance, even once you have the PDB file for HIV Protease, you need to make decisions about how to represent Hydrogens on some of the more volatile amino acids, based on such considerations as PH.

Also, you mention having the PDB file for HIV. Do you mean to say a specific HIV protein? I guessed protease because that's very common. Simulating an entire HIV capsid is a massive task (see: http://www.isgtw.org/feature/64-million-atom-simulation-%E2%80%93-new-weapon-against-hiv). Additionally, performing a binding affinity experiment in the context of an entire capsid is unnecessary - generally these simulations only include the protein and small molecule in question. Although it's possible that interactions from other proteins may affect the binding affinity, performing this sort of simulation across an entire capsid would be impossible, because you'd need an extremely long simulation at a massive scale to account for the flexibility and size of the system.

Best,
Tom Weingarten

On Monday, March 30, 2015 at 10:39:25 PM UTC-4, Gokula Krishna wrote:
I have a Molecule in SMILES format, eg., Zidovudine: Cc1cn(c(=O)[nH]c1=O)[C@H]2C[C@@H]([C@H](O2)CO)N=[N+]=[N-]
And the PDB file for HIV. How to calculate binding affinity computationally? Is there any API?

On Friday, March 13, 2015 at 11:13:56 PM UTC+5:30, Gokula Krishna wrote:
Hello there,

I am a python programmer who is interested in automating the drug simulation process. I taught myself about the basics of:

1. Molecular Dynamics
2. Pharmaco Kinetics
3. Pharmaco Dynamics
4. PyMOL

I am planning to create a program that generates all possible combinations of chemicals for the small molecule drug for a given protein. I could not find any source related to the following:

1. How to generate all possible combination of chemicals of drug for the protein? And how the generated chemicals should be represented for the protein-chemical Interaction (chemical structure like CH4 or other format)? 
2. How to determine whether the given chemical is an agonist or antagonist computationally? And how to determine whether the viral protein is deactivated for the particular chemical?
3. An example on interaction between a simple viral protein and a chemical drug which deactivates it.

Thanks,

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Re: [DIYbio] Biohacking gray hair

Experimental treatment for Lupus, was a genetic therapy.  aftermath included his grey hair turning black again and several titanium implants becoming absorbed by his body, leading to heavy metal issues. 

On Mon, Mar 30, 2015 at 7:54 PM, Katherine Gordon <kthrngordon@gmail.com> wrote:
Hi  Andalib Samandari,

I would enjoy learning more about your comment you added to a a fellow DIY bio concerning the death of hair folliciles:

....."but I only know one method thats ever regenerated the dead cels, and it wasn't supposed to."  Really? Do tell!

Kate 

On Sat, Mar 28, 2015 at 9:26 AM, Andalib Samandari <amsamandari@gmail.com> wrote:
Hey Alexander!

Also, Mike's right about the melanocytes, but theoretically we could control how late the melanocytes die off through genetic engineering (so they produce the colored melanin longer). This is supported by the fact that twins almost always have similar hair greying patterns, suggesting a link to genetics.

On Tuesday, March 10, 2015 at 1:44:26 PM UTC-4, alexander hollins wrote:
I believe it generally ties into the death of the cells responsible for pigment development, and has a pattern similar to death of follicles in male pattern.   There are some prevention methods to keep those cells alive longer, but I only know one method thats ever regenerated the dead cels, and it wasn't supposed to. 


On Tue, Mar 10, 2015 at 9:40 AM, Mike Petersen <mike...@googlemail.com> wrote:
Hi DIYbio community,

I was wondering if there could be any "system-biological" approach to "cure gray hair" and prevent the hair from losing its colour.
What is known about the genetic and the epigenetic mechanisms that cause the colour loss of our hair?
There are some interesting patterns, for example the fact that usually the hair around the temples starts to lose its colors first.
Which expression patterns can be linked to these observations?
And what would be promising ideas to interact with these mechanisms?

Thank you for your ideas

Mike

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Re: [DIYbio] Biohacking gray hair

Hi  Andalib Samandari,

I would enjoy learning more about your comment you added to a a fellow DIY bio concerning the death of hair folliciles:

....."but I only know one method thats ever regenerated the dead cels, and it wasn't supposed to."  Really? Do tell!

Kate 

On Sat, Mar 28, 2015 at 9:26 AM, Andalib Samandari <amsamandari@gmail.com> wrote:
Hey Alexander!

Also, Mike's right about the melanocytes, but theoretically we could control how late the melanocytes die off through genetic engineering (so they produce the colored melanin longer). This is supported by the fact that twins almost always have similar hair greying patterns, suggesting a link to genetics.

On Tuesday, March 10, 2015 at 1:44:26 PM UTC-4, alexander hollins wrote:
I believe it generally ties into the death of the cells responsible for pigment development, and has a pattern similar to death of follicles in male pattern.   There are some prevention methods to keep those cells alive longer, but I only know one method thats ever regenerated the dead cels, and it wasn't supposed to. 


On Tue, Mar 10, 2015 at 9:40 AM, Mike Petersen <mike...@googlemail.com> wrote:
Hi DIYbio community,

I was wondering if there could be any "system-biological" approach to "cure gray hair" and prevent the hair from losing its colour.
What is known about the genetic and the epigenetic mechanisms that cause the colour loss of our hair?
There are some interesting patterns, for example the fact that usually the hair around the temples starts to lose its colors first.
Which expression patterns can be linked to these observations?
And what would be promising ideas to interact with these mechanisms?

Thank you for your ideas

Mike

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Required || Business Analyst || Jacksonville, FL || 6+ Months || Phone And F2F/Skype

Dear Partner

Hope you are doing great!!

Please go through the requirement and let me know if you have any consultant for the same position.

Position:  Business Analyst
Location: Jacksonville, FL/local

6+ Months Contract

Phone And F2F/Skype

 

·         The Analyst must be able to develop using Visual Basic for Application (VBA).

·         As a successful Business Analyst you will need to develop an in-depth knowledge of your business area in order to assess and manage the effect of requested business changes.

·         You will also liaise with business leaders and frontline staff to understand, document and prioritize business requirements across affected business areas.

·         Success will be measured by your ability to develop complete and accurate business models including process, data and organization changes.

 

Principal Duties & Responsibilities:

Specific tasks associated with performing this job As a Analyst, you will work directly with customers to collect and evaluate data using MS Access, Excel, VBA development and SQL querying.

The Analyst will also leverage an existing SQL Server Database and multiple Access databases to enter, update, and mine existing data points.

The Analyst must be able to develop using Visual Basic for Application (VBA). This is a hard requirement. Candidates without VBA experience cannot be considered. Develop project initiation documents defining high-level scope.

Develop business requirements document describing the project's objectives and listing the requirements for each business area prioritized into mandatory, desirable and optional categories. Requirements will have an associated business case which will allow the project leaders to make informed decisions about delivery of the project. Create business cases defining the costs and benefits associated with requested changes. Create user Acceptance test plans.

Thanks and Regards,

Arpit Arora | Technical Recruiter

Technology Resource Group Inc. 
3736 Hillsdale Court Santa Clara, CA 95051

Office:408-709-1760 Ext : 961, Cell: 909-859-1312

Gtalk: Arpittechwire | Fax: 408-884-2409

Arpit@tresourceinc.com | www.tresourceinc.com

 

 

 

 

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