Urgent Need :: Sr. Python Developer , WA

HI ,

Urgent requirement

Title   : Sr. Python Developer

Location  : Bellevue , WA

Duration   : 12 months contract

Skills Required:

·                   Must have 8+ years of IT experience.

·                   Extensive coding skills

·                   Experience in coding for large enterprise products using good Object Oriented programming practices and Design Patterns.

·                   Must have experience with Java/JavaScript/.NET, REST API

·                   Hands-on experience in Python, Open source framework

·                   Should have experience with Linux

 

Thanks & Regards

Praveen Kumar

SRI Tech Solutions Inc.

praveen@sritechsolutions.com

(T) 1-813-423-6500 X Ext: 149 (M) 248-462 -7719  (F) 813.423.6520

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[DIYbio] Re: Freedom Evo Tecan Troubleshooting - HELP!

Hi Ana,

I think I know what the problem is,

Email me and I can work with you to resolve the situation

Thanks,

Daniel

On Monday, June 5, 2017 at 1:30:19 PM UTC-4, Ana Santos wrote:
Hi diybio community!

I was wondering if anyone out there has experience with the Freedom Evo Tecan Robot. 

We have ours for a few years (>5) and no one has good memories of working with it. And basically, we're just trying to use it to read 96 well plates (between 8 and 12) for 24h.

Everything starts out OK, it runs for about 6h and afterward it just stops working showing messages like "Your run has been stopped" or "Your run was aborted". No red light blinking. No alarm. 

However, if we hit the recover bottom, it re-starts working, as if nothing had happened. If we're really lucky, the run then proceeds until the end, but normally it keeps crashing systematically afterwards.

The only pattern we've been able to detect is that the robot normally stops working when the plate is inside the plate reader.

The technicians from Tecan are now almost part of the lab, because of all the problems we have with the Robot, but they never manage to tell us exactly is the problem. Whatever they do (switch a cable, tweak the software, plug/unplug all machinery) works for a couple of hours but we then go back to the same original problem.

We're just desperate to know if someone else has faced the same problem or if it is a problem with our machine.

I look forward to your feedback.

Best regards and DO GREAT SCIENCE!

Ana

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Exclusive Opening || Pentaho/Data Modeling Delivery Lead

Dear Partner,

Hope you are doing great!!

Please go through the requirement and let me know if you are having any consultant for this position.

Please share me the profile asap as this is a very hot requirement.

 

Pentaho/Data Modeling Delivery Lead

San Jose, CA

6+ Months

Primary Skill: Pentaho ETL Tool (Kettle) 
Develop quality, reliable and fault tolerant data solutions based on internal and external customers/users' requirements, applying best practices and new trends/technologies all along the solution lifecycle. ETL tool of preference is Pentaho Kettle (PDI) accompanied by Pentaho Report Designer, Pentaho Business Analytics and other open source tools, integrating them with local and cloud based resources (AWS EC2, Redshift, Lambda, DynamoDB, etc.).
Integration of external data into our data warehouse using REST API and web services among others.
Delivery of data to internal customers and external vendors through different data formats and delivery methods.
Provide daily monitoring, management, troubleshooting and issue resolution to existing and new data solutions and systems' interfaces affected by them.
Follow and implement Agile Best Practices for Data Warehousing, including solution versioning through git.
Support and develop BI solutions through Pentaho Business Analytics

 

Best Regards,

 

Arpit Arora

Pyramid Consulting, Inc.


Desk Phone: 415-943-9386 Email : Arpit.arora@pyramidci.com  |  Linked in: https://www.linkedin.com/in/arpit-arora-55793655  | Web http://www.pyramidci.com/staffing-home

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Re: [DIYbio] Re: 4-tube incubator and optical density meter arduino shield


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On Wed, Jun 28, 2017 at 5:34 PM, Evgeny Vlasov <vlasov.ca@gmail.com> wrote:
Hi!
That is one very interesting link, exactly what I was looking about. Thank you so much.
Link itself is broken but .pdf is searchable. Tomorrow I am going to nag lab's bacteria guy with this project. 

Cheers

Evgeny


On Thursday, October 7, 2010 at 4:34:43 PM UTC-6, ByoWired wrote:


On Oct 7, 5:46 pm, Mackenzie Cowell <m...@diybio.org> wrote:
> I'd like to build a simple incubator+OD meter for up to 4 microcentrifuge
> tubes....

My first quick comment is that you might have a look at the following:

http://soslab.ee.washington.edu/mw/images/e/ef/EE449_SP10_Group6_MS5_Report.pdf

In that, they used LEDs and a Light-to-Frequency Converter called a
TSL230 which I think they interfaced with the Arduino.  They seem
confident in their results.
I'm in the process now of testing an OD system using a similar set-up,
but it's taking OD readings and some other optical properties through
a syringe, not microcentrifuge tubes.  Also, I'm not familiar with the
Arduino and have built my system around the Propeller microcontroller
- sorry.  I have an incubator built around the syringes using non-
inductive resistors, but I haven't yet tested the incubator portion
yet.  I have digital thermometers using DS18B20's.  Although I've
heard of people using PCB traces for heating, it seems to me it might
be easier to use resistors because they can be positioned around the
microtubes without dramas.

Anyway that's it for now.
hope that helps get you started,
Mark

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[DIYbio] Re: 4-tube incubator and optical density meter arduino shield

Hi!
That is one very interesting link, exactly what I was looking about. Thank you so much.
Link itself is broken but .pdf is searchable. Tomorrow I am going to nag lab's bacteria guy with this project. 

Cheers

Evgeny


On Thursday, October 7, 2010 at 4:34:43 PM UTC-6, ByoWired wrote:


On Oct 7, 5:46 pm, Mackenzie Cowell <m...@diybio.org> wrote:
> I'd like to build a simple incubator+OD meter for up to 4 microcentrifuge
> tubes....

My first quick comment is that you might have a look at the following:

http://soslab.ee.washington.edu/mw/images/e/ef/EE449_SP10_Group6_MS5_Report.pdf

In that, they used LEDs and a Light-to-Frequency Converter called a
TSL230 which I think they interfaced with the Arduino.  They seem
confident in their results.
I'm in the process now of testing an OD system using a similar set-up,
but it's taking OD readings and some other optical properties through
a syringe, not microcentrifuge tubes.  Also, I'm not familiar with the
Arduino and have built my system around the Propeller microcontroller
- sorry.  I have an incubator built around the syringes using non-
inductive resistors, but I haven't yet tested the incubator portion
yet.  I have digital thermometers using DS18B20's.  Although I've
heard of people using PCB traces for heating, it seems to me it might
be easier to use resistors because they can be positioned around the
microtubes without dramas.

Anyway that's it for now.
hope that helps get you started,
Mark

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Exclusive Opening || Technical Business Analyst

Dear Partner,

Hope you are doing great!!

Please go through the requirement and let me know if you are having any consultant for this position.

Please share me the profile asap as this is a very hot requirement.

 

Technical Business Analyst

San Ramon, CA

6+ Months

 

Primary Skill: SQL

Excellent communication and analytical skills.

Understand business requirement and work with business users.

Excellent troubleshooting skills.

experience on UNIX platform.

Experience in writing SQL queries and related tools.

 

 

Best Regards,

 

Arpit Arora

Pyramid Consulting, Inc.


Desk Phone: 415-943-9386 Email : Arpit.arora@pyramidci.com  |  Linked in: https://www.linkedin.com/in/arpit-arora-55793655  | Webhttp://www.pyramidci.com/staffing-home

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We Find Hidden Talent

 

 

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Exclusive Opening || IOS Developer || USC / GC / H1B / EAD-GC

Dear Partner,

Hope you are doing great!!

Please go through the requirement and let me know if you are having any consultant for this position.

Please share me the profile asap as this is a very hot requirement.

 

IOS Developer

Piscataway, NJ

6+ Months

 

Primary Skills: Objective C, COCOA, SWIFT, X-code, experience on java in past would be preferred

 

Best Regards,

 

Arpit Arora

Pyramid Consulting, Inc.


Desk Phone: 415-943-9386 Email : Arpit.arora@pyramidci.com  |  Linked in: https://www.linkedin.com/in/arpit-arora-55793655  | Web http://www.pyramidci.com/staffing-home

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We Find Hidden Talent

 

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Exclusive Opening || Data Scientist with Big Data Experience

Dear Partner,

Hope you are doing great!!

Please go through the requirement and let me know if you are having any consultant for this position.

Please share me the profile asap as this is a very hot requirement.

 

Data Scientist with Big Data Experience

Sunnyvale, CA

6+ Months

 

 

PRIMARY SKILL: Big Data Expert

FOR THIS ROLE: Concentrate on Strategic/Consulting + Technology type of profiles, i.e., Big Data Experience along with statistical background.

Hands on Big Data Expert with hands on implementation experience in Data Streaming platforms.

Organize and "wrangle" large datasets

8 yrs experience is preferred

Building real-time data pipelines

Cluster analysis

Segmentation and churning of real-time datasets

Experience with Apache Kafka

Experience with other Distributed Streaming data platforms – to evaluate the current ecosystem the problem in hand and suggest the best platform to implement with ability to do quick POC and show the benefits.

 

Best Regards,

 

Arpit Arora

Pyramid Consulting, Inc.


Desk Phone: 415-943-9386 Email : Arpit.arora@pyramidci.com  |  Linked in: https://www.linkedin.com/in/arpit-arora-55793655  | Webhttp://www.pyramidci.com/staffing-home

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[DIYbio] Re: I'm a beginner and interested in learning Biohacking but don't know how to and where to

https://www.youtube.com/watch?v=Ds8ZFzOwGeI

Try replicating the Thought Emporium's project on the Scoby Project that's the place I started but that's because I have some lab expierence in my belt as a Biology major.

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Exclusive Opening || Apriso FlexNet Consultant

Dear Partner,

Hope you are doing great!!

Please go through the requirement and let me know if you are having any consultant for this position.

Please share me the profile asap as this is a very hot requirement.


Apriso Flex Net Consultant

Sunnyvale, CA

6+ Months

 

Primary Skill: Apriso FlexNet

Experience on Apriso FlexNet MES Experience in developing screens on Process Builder Knowledge of standard Apriso FlexNet modules like Production, Warehousing and Monitoring. Experience in PL/SQL programming Knowledge of MES Design, Development, Testing, Support & Implementation process Manufacturing Domain Expertise preference in Manufacturing Processes Understanding of Manufacturing IT systems (ERP/MES) will be an added advantage Creating Technical Design Document Development - Following standard coding practices Good communication skills and team player.

 

Best Regards,

 

Arpit Arora

Pyramid Consulting, Inc.


Desk Phone: 415-943-9386 Email : Arpit.arora@pyramidci.com  |  Linked in: https://www.linkedin.com/in/arpit-arora-55793655  | Webhttp://www.pyramidci.com/staffing-home

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We Find Hidden Talent

 

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Title : Senior Python Developer / Lead in Bellevue , WA

Hi,

 

Please find the below position and respond back to me with your consultants profiles

 

Title : Senior Python Developer 

Location : Bellevue , WA

Duration : 6-12 + months contract

 

Skills Required:

  • Must have 8+ years of IT experience.
  • Extensive coding skills
  • Experience in coding for large enterprise products using good Object Oriented programming practices and Design Patterns.
  • Must have experience with Java/JavaScript/.NET, REST API
  • Hands-on experience in Python, Open source framework
  • Should have experience with Linux

 

 

--

Thanks and Best Regards,

Praveen Kumar

SRI Tech Solutions Inc.

praveen@sritechsolutions.com

(T) 1-813-423-6500 X Ext: 149 (M) 248-462 -7719 (F) 813.423.6520

__________________________________________________________________________________________________

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Re: [DIYbio] Paralens fluorescence microscope objective for Hoechst and DAPI?

Hey,

DAPI/Hoechst excitation require UV light, so if there's a UV filter in front of your camera, you'll see only the fluorescence signal.
However, common glass is not transparent to UV, so it might be that if you're using cheap glassware in the beam path you'll have a problem.

The 100X objective does not let much light go through it, but Hoechst and DAPI have a very high signal-to-noise ratio, they're very bright, so even in an hacked system there shouldn't be much of a problem.

You can find microscope cameras around, but does your microscope allow for a camera? Is it a trinocular? You can spend additional money for a camera and a C-Mount and hack light source and filters, but probably at that point the cost becomes too high.
If you wanna go DIY, then I would hack the system completely. You can just stick a raspberry Pi camera in front of the ocular (possibly with a 20X magnification if you have it) and it should come with a UV filter (I don't know how much useful). 

There are UV leds that you could use for illumination, you have to make sure that they would provide enough light.
Filters in your case is easy, you can find UV filters.

The solution with the UV light source in one ocular makes sense and it's probably the only one available to you (beside the paralens): you need the light to point away from your eyes and have the fluorescence reflected back, differently to what you are probably used to in brightfield.

To get more info on how to setup something like this, you can have a look at this paper here: http://biorxiv.org/content/biorxiv/early/2017/03/31/122812.full.pdf

If you're going to experiment, please keep in mind that you don't want UV light in your eyes. Be careful.

Best,

ukitel

On Saturday, 24 June 2017 09:36:52 UTC+2, Mike wrote:
Abizar,

Thank you - yes I'd be very interested in trying the setup you suggest - please can you post an image? I have Zeiss Standard 25 binocular microscope. The key thing is that I need a sharp fluorescence image with a 100x oil objective.

- Is the setup you suggest compatible with this type of objective?
- Where can I get the required parts and how do I make them fit my microscope (LED, filters, camera)?
- Can I acquire LED lighting that will provide for UV illumination (for use with DAPI, Hoechst)?

Roninlaw,

The price of the core Paralens Advance system with a single objective lens type is approximately £900 including VAT.

Thanks guys,

Mike

On Friday, June 23, 2017 at 11:15:21 PM UTC+1, Roninlaw wrote:
Price?

On Fri, Jun 23, 2017 at 2:25 PM, Mike <alanmb...@gmail.com> wrote:
Hello all,

As a cheap alternative to a fluorescence microscope for my home lab, I am considering the Paralens Advance screw-on objective lens.

(Please see page 9 of the PDF for an illustration of light spectra)

The Paralens produces excitation at 385-480nm and emission at 480nm (as far as I can tell, please see PDF above!). [b]Question[/b]: Will this device work with Hoechst 33258 and DAPI stain (spectra as below)?

- Hoechst 33258: excitation: 352, emission: 461
- DAPI: excitation: 358, emission: 461

Thank you,

Mike

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Re: [DIYbio] Paralens fluorescence microscope objective for Hoechst and DAPI?

Abizar,

Thank you - yes I'd be very interested in trying the setup you suggest - please can you post an image? I have Zeiss Standard 25 binocular microscope. The key thing is that I need a sharp fluorescence image with a 100x oil objective.

- Is the setup you suggest compatible with this type of objective?
- Where can I get the required parts and how do I make them fit my microscope (LED, filters, camera)?
- Can I acquire LED lighting that will provide for UV illumination (for use with DAPI, Hoechst)?

Roninlaw,

The price of the core Paralens Advance system with a single objective lens type is approximately £900 including VAT.

Thanks guys,

Mike

On Friday, June 23, 2017 at 11:15:21 PM UTC+1, Roninlaw wrote:
Price?

On Fri, Jun 23, 2017 at 2:25 PM, Mike <alanmb...@gmail.com> wrote:
Hello all,

As a cheap alternative to a fluorescence microscope for my home lab, I am considering the Paralens Advance screw-on objective lens.

(Please see page 9 of the PDF for an illustration of light spectra)

The Paralens produces excitation at 385-480nm and emission at 480nm (as far as I can tell, please see PDF above!). [b]Question[/b]: Will this device work with Hoechst 33258 and DAPI stain (spectra as below)?

- Hoechst 33258: excitation: 352, emission: 461
- DAPI: excitation: 358, emission: 461

Thank you,

Mike

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Re: [DIYbio] Paralens fluorescence microscope objective for Hoechst and DAPI?

An alternative is to place your illuminating led and filter on one eyepiece of a binocular head. And a filter and camera on the other eyepiece. The led beam is semi focused on the specimen through the objective. Fluorescence light goes up the optical path and is divided equally into the eyepiece though only the camera sees the fluorescence image.
Can send you an image of this setup if it helps.

On Jun 23, 2017 2:25 PM, "Mike" <alanmbeattie@gmail.com> wrote:
Hello all,

As a cheap alternative to a fluorescence microscope for my home lab, I am considering the Paralens Advance screw-on objective lens.

(Please see page 9 of the PDF for an illustration of light spectra)

The Paralens produces excitation at 385-480nm and emission at 480nm (as far as I can tell, please see PDF above!). [b]Question[/b]: Will this device work with Hoechst 33258 and DAPI stain (spectra as below)?

- Hoechst 33258: excitation: 352, emission: 461
- DAPI: excitation: 358, emission: 461

Thank you,

Mike

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Re: [DIYbio] Paralens fluorescence microscope objective for Hoechst and DAPI?

Price?

On Fri, Jun 23, 2017 at 2:25 PM, Mike <alanmbeattie@gmail.com> wrote:
Hello all,

As a cheap alternative to a fluorescence microscope for my home lab, I am considering the Paralens Advance screw-on objective lens.

(Please see page 9 of the PDF for an illustration of light spectra)

The Paralens produces excitation at 385-480nm and emission at 480nm (as far as I can tell, please see PDF above!). [b]Question[/b]: Will this device work with Hoechst 33258 and DAPI stain (spectra as below)?

- Hoechst 33258: excitation: 352, emission: 461
- DAPI: excitation: 358, emission: 461

Thank you,

Mike

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[DIYbio] Paralens fluorescence microscope objective for DAPI and Hoechst?

Hello all,

As a cheap alternative to a fluorescence microscope for my home lab, I am considering the Paralens Advance screw-on objective lens.

http://www.druckerdiagnostics.com/media/wysiwyg/paralens-advance/PLA_Manual_No_Spreads_.pdf
(Please see page 9 of the PDF for an illustration of light spectra)

The Paralens produces excitation at 385-480nm and emission at 480nm (as far as I can tell, please see PDF above!). [b]Question[/b]: Will this device work with Hoechst 33258 and DAPI stain (spectra as below)?

- Hoechst 33258: excitation: 352, emission: 461
- DAPI: excitation: 358, emission: 461

Thank you,

Mike

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[DIYbio] Paralens fluorescence microscope objective for Hoechst and DAPI?

Hello all,

As a cheap alternative to a fluorescence microscope for my home lab, I am considering the Paralens Advance screw-on objective lens.

http://www.druckerdiagnostics.com/media/wysiwyg/paralens-advance/PLA_Manual_No_Spreads_.pdf
(Please see page 9 of the PDF for an illustration of light spectra)

The Paralens produces excitation at 385-480nm and emission at 480nm (as far as I can tell, please see PDF above!). [b]Question[/b]: Will this device work with Hoechst 33258 and DAPI stain (spectra as below)?

- Hoechst 33258: excitation: 352, emission: 461
- DAPI: excitation: 358, emission: 461

Thank you,

Mike

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