Exclusive Opening || SAP SM Consultant

Dear Partner,

Hope you are doing great!!

Please go through the requirement and let me know if you are having any consultant for this position.

Please share me the profile asap as this is a very hot requirement.

SAP SM Consultant

Sunnyvale, CA

6+ months

 

 

PRIMARY SKILL: SAP Service Management
6-8 years on SAP Service Management or Customer service (at least 2-4 years of SAP SD experience).
Ability to read technical code is strongly preferred.
Candidate should be able to independently execute on designs and be able to provide alternatives for challenging business requirements.
Expected areas of knowledge include strong process knowledge of Repairs / Service notifications, Warranty and Contracts.
Integration capabilities and cross modular capability in SAP SD and MM/LE/PM
Should have worked in minimum of 2-4 rollouts/implementation projects
Experience and ability to communicate with various stake holders

 

 

 

 

Best Regards,

 

Arpit Arora

Pyramid Consulting, Inc.


Desk Phone: 415-943-9386 Email : Arpit.arora@pyramidci.com  |  Linked in: https://www.linkedin.com/in/arpit-arora-55793655  | Webhttp://www.pyramidci.com/staffing-home

cid:image009.jpg@01D02C05.4FFC5560

We Find Hidden Talent

 

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Re: [DIYbio] Gel Red questions

So are there cheaper sources?

http://www.sigmaaldrich.com/catalog/product/sial/390062?lang=de&region=AT 
Like this







On Sunday, April 23, 2017 at 10:03:09 PM UTC+2, John Ladasky wrote:


On Tuesday, March 12, 2013 at 12:03:42 PM UTC-7, Nathan McCorkle wrote:
Yeah, that's why big companies and universities don't switch.

EtBr is molecularly known (no one really knows exactly what the gelRed
and sybrSafe molecules are, we know what classes they're in or one
that was described in one patent, etc)

I'm pretty sure that this information cannot be kept secret.  To obtain a chemistry patent, and to publish an accurate MSDS, the structure of the novel active compound must be known and disclosed.

I remember that SybrSafe is in the cyanine dye family, a descendant of Thiazole Orange.  If you look at the Wikipedia article for SybrSafe, a structure is published, and it sure looks like a cyanine dye to me.

 
Wikipedia entries are also available for GelGreen and GelRed.  They look like acridine orange and ethidium dimers, respectively, with a peptide-ether linker between the individual fluorophores.


The idea of making ethidium dimers is not new.  An ethidium dimer was available in 1990.  It's still available.  The linker differs from the GelRed linker, but otherwise the two molecules are quite similar.



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[DIYbio] Re: Eppendorf 5415 centrifuge: How to remove the rotor?

I recently had this problem. I found that standard size sharpies fit perfectly in the 1.5ml microcentrifuge holes. There was no reverse thread. Standard righty-tighty, lefty-loosey.

Mine was really stuck on there, but after cranking it way harder than I should have to, it popped free.

I have another eppy that the rotor is totally stuck to the shaft. The screw is loose, but it WILL NOT come loose. Soaked it in WD-40 even. No dice.

On Friday, August 19, 2016 at 2:18:51 PM UTC-4, jarlemag wrote:
I recently bought an Eppendorf 5415D centrifuge, and have tried to remove the rotor for cleaning and inspection. I don't have a rotor key of the kind that is sold by Eppendorf, but the item description at http://www.capitolscientific.com/Eppendorf-022667181-Rotor-Key-for-Microcentrifuge-Models-5415-D-5415-R-10mm-Socket-Wrench seems to indicate it's just a standard 10 mm socket wrench, so I bough one of those. (https://www.amazon.com/gp/product/B0028N57SW). I attempted to use the socket wrench on the hexagonal part in the centre (see the picture), but that just makes the whole rotor turn. I tried to keep the rotor in place while turning the wrench, but that didn't work either (I couldn't keep the rotor still).
If I try to lift the rotor upwards, the centrifuge hangs from it and I'm worried about damagaging it so I didn't try that very hard.

The centrifuge manual (http://www.nist.gov/ncnr/upload/Eppendorf_5415R_Centrifuge_Manual.pdf) doesn't go into detail about how to remove the motor. It mentions bending the rotor to one side if it's stuck, but how do I know if it's stuck in the way which is solved by doing that? 

What do I need to do?


Best regards,
JP

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Re: [DIYbio] Anti-Tick Bio-Weapon?

Yeah because bacteria and virusses won't have point mutations that make the CRISPR gene drive's guides inactive... Nature isn't a rigid system

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Re: [DIYbio] Anti-Tick Bio-Weapon?

Ticks and other parasites perform an ecological service, by creating a route for nutrients back down the foodchain from top-level consumers (these days, mostly mammals). Kind of like ecological taxation: parasites pull eco-"wealth" down where lower tiers can access it, so a nutrient monopoly doesn't occur.

Are they essential in all/most/any ecosystems in this role? I'm not an ecologist, so I can't accurately say. But it's lower risk to kill a disease than a carrier parasite, IMO.

When humans are involved, I know where my loyalties lie: kill the bugs (go Oxytech!), but if a parallel strategy like "immunise the ticks against Lyme with a gene drive!" exists, I'd err in that direction instead.

On 25 April 2017 01:42:17 GMT+01:00, Cory Geesaman <cory@geesaman.com> wrote:
Interesting article, but it doesn't really seem capable of eradicating Lyme disease across the mainland US without a massive undertaking.  Is there anything which could act like an infectious form of CRISPR where it spreads harmlessly through mice but rewrites tick DNA with some critical flaw like sterility?

On Monday, April 24, 2017 at 4:45:10 PM UTC-4, balduino wrote:
There is the fairly famous idea talked about by Kevin Esvelt on the use of gene drives on the white-footed mouse,  This would appently eradicate Lyme disease

http://www.newyorker.com/magazine/2017/01/02/rewriting-the-code-of-life

On Mon, Apr 24, 2017 at 4:26 PM, Cory Geesaman <co...@geesaman.com> wrote:
I was reading this article discussing how this year is expected to have above average numbers of ticks with associated Lyme infections.  It got me wondering if there is any basis for a virus or bacterial infection which might be used to wipe out an entire species, namely ticks.  It seems like a benign mouse STD which either sterilizes or kills ticks which infect it could work.  Is there already research being done into such things?  Are there mechanisms to ensure a virus will only adversely impact a specific species?  Would they work at the DNA level (i.e. seeking out a specific sequence) or require that a specific protein exist in the target species?  I'm aware this could be really dangerous if it mutated the wrong way and have no intention of attempting it, just curious.

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Re: [DIYbio] Anti-Tick Bio-Weapon?

Interesting article, but it doesn't really seem capable of eradicating Lyme disease across the mainland US without a massive undertaking.  Is there anything which could act like an infectious form of CRISPR where it spreads harmlessly through mice but rewrites tick DNA with some critical flaw like sterility?

On Monday, April 24, 2017 at 4:45:10 PM UTC-4, balduino wrote:
There is the fairly famous idea talked about by Kevin Esvelt on the use of gene drives on the white-footed mouse,  This would appently eradicate Lyme disease

http://www.newyorker.com/magazine/2017/01/02/rewriting-the-code-of-life

On Mon, Apr 24, 2017 at 4:26 PM, Cory Geesaman <co...@geesaman.com> wrote:
I was reading this article discussing how this year is expected to have above average numbers of ticks with associated Lyme infections.  It got me wondering if there is any basis for a virus or bacterial infection which might be used to wipe out an entire species, namely ticks.  It seems like a benign mouse STD which either sterilizes or kills ticks which infect it could work.  Is there already research being done into such things?  Are there mechanisms to ensure a virus will only adversely impact a specific species?  Would they work at the DNA level (i.e. seeking out a specific sequence) or require that a specific protein exist in the target species?  I'm aware this could be really dangerous if it mutated the wrong way and have no intention of attempting it, just curious.

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Re: [DIYbio] Anti-Tick Bio-Weapon?

Why would you aim for a disease carried by a parasite instead of the parasite itself?

On Monday, April 24, 2017 at 5:05:14 PM UTC-4, Maria Chavez wrote:
Also of note there IS a vaccine for Lyme disease.  Too bad you cant
have it *sigh*.

http://legacy.wbur.org/2012/06/27/lyme-vaccine

On Mon, Apr 24, 2017 at 1:45 PM, Steven Stowell <scst...@gmail.com> wrote:
> There is the fairly famous idea talked about by Kevin Esvelt on the use of
> gene drives on the white-footed mouse,  This would appently eradicate Lyme
> disease
>
> http://www.newyorker.com/magazine/2017/01/02/rewriting-the-code-of-life
>
> On Mon, Apr 24, 2017 at 4:26 PM, Cory Geesaman <co...@geesaman.com> wrote:
>>
>> I was reading this article discussing how this year is expected to have
>> above average numbers of ticks with associated Lyme infections.  It got me
>> wondering if there is any basis for a virus or bacterial infection which
>> might be used to wipe out an entire species, namely ticks.  It seems like a
>> benign mouse STD which either sterilizes or kills ticks which infect it
>> could work.  Is there already research being done into such things?  Are
>> there mechanisms to ensure a virus will only adversely impact a specific
>> species?  Would they work at the DNA level (i.e. seeking out a specific
>> sequence) or require that a specific protein exist in the target species?
>> I'm aware this could be really dangerous if it mutated the wrong way and
>> have no intention of attempting it, just curious.
>>
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Re: [DIYbio] Anti-Tick Bio-Weapon?

Also of note there IS a vaccine for Lyme disease. Too bad you cant
have it *sigh*.

http://legacy.wbur.org/2012/06/27/lyme-vaccine

On Mon, Apr 24, 2017 at 1:45 PM, Steven Stowell <scstowell@gmail.com> wrote:
> There is the fairly famous idea talked about by Kevin Esvelt on the use of
> gene drives on the white-footed mouse, This would appently eradicate Lyme
> disease
>
> http://www.newyorker.com/magazine/2017/01/02/rewriting-the-code-of-life
>
> On Mon, Apr 24, 2017 at 4:26 PM, Cory Geesaman <cory@geesaman.com> wrote:
>>
>> I was reading this article discussing how this year is expected to have
>> above average numbers of ticks with associated Lyme infections. It got me
>> wondering if there is any basis for a virus or bacterial infection which
>> might be used to wipe out an entire species, namely ticks. It seems like a
>> benign mouse STD which either sterilizes or kills ticks which infect it
>> could work. Is there already research being done into such things? Are
>> there mechanisms to ensure a virus will only adversely impact a specific
>> species? Would they work at the DNA level (i.e. seeking out a specific
>> sequence) or require that a specific protein exist in the target species?
>> I'm aware this could be really dangerous if it mutated the wrong way and
>> have no intention of attempting it, just curious.
>>
>> --
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>> https://groups.google.com/d/forum/diybio?hl=en
>> Learn more at www.diybio.org
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Re: [DIYbio] Anti-Tick Bio-Weapon?

There is the fairly famous idea talked about by Kevin Esvelt on the use of gene drives on the white-footed mouse,  This would appently eradicate Lyme disease

http://www.newyorker.com/magazine/2017/01/02/rewriting-the-code-of-life

On Mon, Apr 24, 2017 at 4:26 PM, Cory Geesaman <cory@geesaman.com> wrote:
I was reading this article discussing how this year is expected to have above average numbers of ticks with associated Lyme infections.  It got me wondering if there is any basis for a virus or bacterial infection which might be used to wipe out an entire species, namely ticks.  It seems like a benign mouse STD which either sterilizes or kills ticks which infect it could work.  Is there already research being done into such things?  Are there mechanisms to ensure a virus will only adversely impact a specific species?  Would they work at the DNA level (i.e. seeking out a specific sequence) or require that a specific protein exist in the target species?  I'm aware this could be really dangerous if it mutated the wrong way and have no intention of attempting it, just curious.

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Re: [DIYbio] Anti-Tick Bio-Weapon?

Google Gene Drive technology.

On Mon, Apr 24, 2017 at 4:26 PM Cory Geesaman <cory@geesaman.com> wrote:
I was reading this article discussing how this year is expected to have above average numbers of ticks with associated Lyme infections.  It got me wondering if there is any basis for a virus or bacterial infection which might be used to wipe out an entire species, namely ticks.  It seems like a benign mouse STD which either sterilizes or kills ticks which infect it could work.  Is there already research being done into such things?  Are there mechanisms to ensure a virus will only adversely impact a specific species?  Would they work at the DNA level (i.e. seeking out a specific sequence) or require that a specific protein exist in the target species?  I'm aware this could be really dangerous if it mutated the wrong way and have no intention of attempting it, just curious.

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[DIYbio] Anti-Tick Bio-Weapon?

I was reading this article discussing how this year is expected to have above average numbers of ticks with associated Lyme infections.  It got me wondering if there is any basis for a virus or bacterial infection which might be used to wipe out an entire species, namely ticks.  It seems like a benign mouse STD which either sterilizes or kills ticks which infect it could work.  Is there already research being done into such things?  Are there mechanisms to ensure a virus will only adversely impact a specific species?  Would they work at the DNA level (i.e. seeking out a specific sequence) or require that a specific protein exist in the target species?  I'm aware this could be really dangerous if it mutated the wrong way and have no intention of attempting it, just curious.

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Re: [DIYbio] Gel Red questions



On Tuesday, March 12, 2013 at 12:03:42 PM UTC-7, Nathan McCorkle wrote:
Yeah, that's why big companies and universities don't switch.

EtBr is molecularly known (no one really knows exactly what the gelRed
and sybrSafe molecules are, we know what classes they're in or one
that was described in one patent, etc)

I'm pretty sure that this information cannot be kept secret.  To obtain a chemistry patent, and to publish an accurate MSDS, the structure of the novel active compound must be known and disclosed.

I remember that SybrSafe is in the cyanine dye family, a descendant of Thiazole Orange.  If you look at the Wikipedia article for SybrSafe, a structure is published, and it sure looks like a cyanine dye to me.

https://en.wikipedia.org/wiki/SYBR_Safe
 
Wikipedia entries are also available for GelGreen and GelRed.  They look like acridine orange and ethidium dimers, respectively, with a peptide-ether linker between the individual fluorophores.

https://en.wikipedia.org/wiki/GelGreen
https://en.wikipedia.org/wiki/GelRed

The idea of making ethidium dimers is not new.  An ethidium dimer was available in 1990.  It's still available.  The linker differs from the GelRed linker, but otherwise the two molecules are quite similar.

https://www.thermofisher.com/order/catalog/product/E1169


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Re: [DIYbio] Why are PCR machines and biotech equipment in general so expensive

Dennis said it perfectly on all 3 counts.

Ujjwal I thought you were going to develop lower cost PCR. What has stopped your efforts from succeeding?

-Josh


-Josh

On Fri, Apr 21, 2017 at 8:47 AM, Dennis Oleksyuk <dennis@oleksyuk.me> wrote:
Because building hardware is harder than building software. It requires more skills, time, and money. That's the main reason which applies to hardware development as a whole.

Because the number of customers who buy scientific equipment is small. Therefore the manufacturer has to divide the development cost between a smaller number of customers.

Because the cost of consumables and labor for solving a particular problem is usually higher than the cost of the machine. Therefore the buyers are more interested in saving in labor and consumable cost rather than hardware prices.


On Fri, Apr 21, 2017 at 8:42 AM Gerald Trost <gerald.trost@mail.com> wrote:

this is only my honest opinion from my experience:

I bought a open source 3d printer - in industry they had
such things for 3 decades - but they costed
several hundred thousands

the open source thing works fine but for 90% of the time
its in maintenance and I am the machine engineer

I think the open source things are not yet reliable enough.
 
Sent: Friday, April 21, 2017 at 1:57 PM
From: "Ujjwal Thaakar" <ujjwalthaakar@gmail.com>
To: DIYbio <diybio@googlegroups.com>
Subject: [DIYbio] Why are PCR machines and biotech equipment in general so expensive
How do the economics work out and why have we not seen bigger companies bring down the process in the advent of open source equipment as well as new startups building low-cost equipment?

 

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Re: [DIYbio] Why are PCR machines and biotech equipment in general so expensive

Anyone here has an example of how results differ on different PCR machines? Like how much and on what parameter because so many researchers have told me that they don't want to disturb their setup!

On Sun, Apr 23, 2017 at 12:26 AM Gerald Trost <gerald.trost@mail.com> wrote:

Hi all!

Andreas, did you build your own thermocycler ?
I only ask because I wonder how many of us have successfully tried it.

In my chest there beat 2 hearts:

one says
"I am DIY enthusiast, I want to be able to do anything on
my own - at least I want to have the know-how"
 
the other heart says:
"I tried to build so many things and always did I end up
as machine engineer and problem solver"

I have seen OpenPCR and many similar projects on youtube
and I still think these are too expensive.

I dont think the peltier and the heat block are enough -
I suspect that rapid cooling and distributing heat
evenly might be the major issues.

Once I find the time I will bend a thin copper pipe to rings
in order to take up the reaction tubes and let some cooling
liquid run through these pipes in order to heat and to cool.
(Arduino drives peltier and a pump ...)

Maybe I am off topic because I am not really that
familiar with lab technology, if so, please let me know.

The basic idea of my design will be to spread heat and
the cooling evenly - as good as possible.

if somebody is intersted then please, suggest a
discussion forum where DIY-builders can discuss
the "Easy LiquidThermoCycler"

Gerald





 
 
Sent: Saturday, April 22, 2017 at 8:11 AM
From: "Mega [Andreas Stuermer]" <masterstorm123@gmail.com>
To: DIYbio <diybio@googlegroups.com>
Subject: Re: [DIYbio] Why are PCR machines and biotech equipment in general so expensive
Demand and supply. Everybody of 8 billion humans wants a smartphone and there is enourmous competition and pressure to make it as cheap as possible.

Way less demand for thermocyclers, so less companies building them. Then theres the grant system so it's not the researchers personal money and nobody says "are you stupid? I'm not gonna pay you 5000$ for a heat block and a peltier" and builds his own

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Chief Everything Officer

Kesar | Linkedin | Twitter

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Re: [DIYbio] Why are PCR machines and biotech equipment in general so expensive


Hi all!

Andreas, did you build your own thermocycler ?
I only ask because I wonder how many of us have successfully tried it.

In my chest there beat 2 hearts:

one says
"I am DIY enthusiast, I want to be able to do anything on
my own - at least I want to have the know-how"
 
the other heart says:
"I tried to build so many things and always did I end up
as machine engineer and problem solver"

I have seen OpenPCR and many similar projects on youtube
and I still think these are too expensive.

I dont think the peltier and the heat block are enough -
I suspect that rapid cooling and distributing heat
evenly might be the major issues.

Once I find the time I will bend a thin copper pipe to rings
in order to take up the reaction tubes and let some cooling
liquid run through these pipes in order to heat and to cool.
(Arduino drives peltier and a pump ...)

Maybe I am off topic because I am not really that
familiar with lab technology, if so, please let me know.

The basic idea of my design will be to spread heat and
the cooling evenly - as good as possible.

if somebody is intersted then please, suggest a
discussion forum where DIY-builders can discuss
the "Easy LiquidThermoCycler"

Gerald





 
 
Sent: Saturday, April 22, 2017 at 8:11 AM
From: "Mega [Andreas Stuermer]" <masterstorm123@gmail.com>
To: DIYbio <diybio@googlegroups.com>
Subject: Re: [DIYbio] Why are PCR machines and biotech equipment in general so expensive
Demand and supply. Everybody of 8 billion humans wants a smartphone and there is enourmous competition and pressure to make it as cheap as possible.

Way less demand for thermocyclers, so less companies building them. Then theres the grant system so it's not the researchers personal money and nobody says "are you stupid? I'm not gonna pay you 5000$ for a heat block and a peltier" and builds his own

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Re: [DIYbio] Glowing plant project fails

Classic Philosophers dilemma "The less you know, the more confident you are" you will be able to deliver ...

On Sat, Apr 22, 2017 at 3:19 AM, David Murphy <murphy.david@gmail.com> wrote:
I'm honestly surprised it failed so totally. I was under the impression that they were basically replicating earlier work that made some very faintly glowing plants.

I expected them to end up shipping something you might just about be able to see glowing in a dark room and end up stuck on the problem of getting it brighter, not utterly bombing.

On Fri, Apr 21, 2017 at 9:20 PM, Cas Smith <charles.cas.smith@gmail.com> wrote:
Kickstarter's recent 'wish list' (Our Design & Tech Team's 'Request for Projects', see: Boundary Pushers section) gives me hope that biotech/synbio/etc. will still be welcomed on their platform. They also recently hosted an event for the Biodesign Challenge. So...think positive thoughts (and advocate), I guess?



On Thursday, April 20, 2017 at 1:00:37 PM UTC-4, Cathal Garvey wrote:
I feel that this project should never have even started, because it's
implausible even on physical grounds. But, having said that.. they
could have just declared it a "fail" and walked away, at least they're
still trying to deliver something that would interest their backers.

I'm not super hopeful, but it's better than nothing. I've got no "skin
in the game" though as I never backed the project to begin with.

Whatever hopes we ever had of getting Kickstarter to stop banning
bioscience projects is slimmer now, though; the credibility is burned
and there hasn't been as much advocacy to lift the ban as there was to
instate it.

For my part, I'll still never back something on Kickstarter as long as
the ban remains. They only introduced it at the behest of the
anti-science swarm, there is no rational basis to it.

--
@onetruecathal / @cat...@quitter.is


On Thu, Apr 20, 2017 at 5:49 PM, John Griessen <jo...@industromatic.com>
wrote:
> On 04/18/2017 08:29 PM, Koeng wrote:
>> I think we all saw this coming.
>
> Oh, I didn't think they would bomb that bad -- maybe just have plants
> that faded after a few sexual reproduction cycles.
>
> $484K spent without money to ship the main reward is pretty bad
> though.
> At least they are going to lay off someone and ship something before
> the money is gone.
>
> If only they had located their project down the street from Sebastian.
> Probably would have had glowing moss graffiti last October and
> shipping glowing
> leaf plants today.
>
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